| establishment of a cell line derived from embryos of the potato tuber moth phthorimaea operculella (zeller). | a cell line from the main insect pest of potatoes in tropical and subtropical areas, phthorimaea operculella (zeller), was obtained from embryoculture. these cells were cultured in grace's modified medium. the cell line, designated ors-pop-93, had a heterogeneous population consisting of spherical and spindle cells with great capacity to adhere and a doubling time of 40 h. they were subcultured for more than 60 passages. their polypeptidic profile was different from profiles of other lepidoptera ... | 1995 | 8826086 |
| laboratory screening of different bacillus thuringiensis strains against certain lepidopteran pests and subsequent field evaluation on the pod boring pest complex of pigeonpea (cajanus cajan). | laboratory evaluation of different bacillus thuringiensis subspecies revealed that b. thuringiensis subsp. kurstaki (ncim 2514) at 10(8) spores/ml concentration caused more than 85% mortality to the neonate larvae of the lepidopteran insects spodoptera litura (f.) and phthorimaea operculella (z.). this strain at 10(10) spores/ml concentration was effective against the major lepidopteran pests comprising the pod boring complex of pigeonpea (cajanus cajan), viz. helicoverpa armigera (h.) and exela ... | 1997 | 9195006 |
| multiplication of a granulosis virus isolated from the potato tuber moth in a new established cell line of phthorimaea operculella. | a newly established cell line was obtained from the culture of embryonic cells of the potato tuber moth phthorimaea operculella in low temperature conditions (19 degrees c) using modified grace's medium supplemented with 10% fetal bovine serum. the population doubling time was about 80 h when cells were cultivated at 19 degrees c and 38 h at 27 degrees c. the cell line had a relatively homogeneous population consisting of various sized spherical cells. the cells were cultivated for more than 25 ... | 1997 | 9338147 |
| occurrence of a common binding site in mamestra brassicae, phthorimaea operculella, and spodoptera exigua for the insecticidal crystal proteins cryia from bacillus thuringiensis. | specific binding to midgut membrane proteins is required for the toxicity of insecticidal crystal proteins (icp) from bacillus thuringiensis. a direct relationship between toxicity and binding has been proposed. it has been hypothesized that sharing of a single receptor by more than one icp could lead to the occurrence of multiple resistance in the event of an alteration in the common receptor. binding of cryia(a), cryia(b) and cryia(c), three structurally related icps, has been studied in phtho ... | 1997 | 9404010 |
| evaluation of potato tuber moth (lepidoptera: gelechiidae) resistance in tubers of bt-cry5 transgenic potato lines. | the potato tuber moth, phthorimaea operculella (zeller), in tropical and subtropical countries, is the most destructive pest of potato, solanum tuberosum l. the larvae attack foliage and tubers in the field and in storage. the purpose of this study was to evaluate the efficacy of a bt-cry5 transgene to control the potato tuber moth in tuber tissues. tuber bioassays using stored (11-12 mo old) and newly harvested tubers of bt-cry5-lemhi russet and bt-cry5-atlantic potato lines showed up to 100% m ... | 2000 | 10826202 |
| population biology of the potato tuber moth, phthorimaea operculella (lepidoptera: gelechiidae), in two potato cropping systems in israel. | the life cycle, within-field distribution, crop damage and impact of natural enemies of the potato tuber moth, phthorimaea operculella (zeller) were investigated in two potato cropping systems. the two systems differed in soil type (sandy vs. loess) which in turn affected the choice of cultivars, irrigation programme, insecticide application method (ground vs. aerial), and planting and harvest times. from mid-april to the end of may, almost twice as many moths were caught in pheromone traps in s ... | 2000 | 11020789 |
| comparative analysis of the granulin regions of the phthorimaea operculella and spodoptera littoralis granuloviruses. | the nucleotide sequence of two cloned restriction fragments encompassing the granulin genes from the granuloviruses of the potato tuber moth, phthorimaea operculella, phopgv, and the egyptian cotton leaf worm, spodoptera littoralis, spligv, have been determined. although both viruses are able to infect the same ph. operculella cell line, their granulins do not cluster in the same phylogenetic branches. phopgv ganulin is closely related to cydia pomonella gv (cpgv) and cryptophlebia leucotreta gv ... | 2000 | 11129630 |
| cloning and characterization of an insecticidal crystal protein gene from bacillus thuringiensis subspecies kenyae. | a sporulating culture of bacillus thuringiensis subsp. kenyae strain hd549 is toxic to larvae of lepidopteran insect species such as spodoptera litura, helicoverpa armigera and phthorimaea operculella, and a dipteran insect, culex fatigans. a 1.9-kb dna fragment, pcr-amplified from hd549 using cryii-gene-specific primers, was cloned and expressed in e. coli. the recombinant protein produced 92% mortality in first-instar larvae of spodoptera litura and 86% inhibition of adult emergence in phthori ... | 2002 | 12357073 |
| choristoneura fumiferana granulovirus p74 protein, a highly conserved baculoviral envelope protein. | a gene that encodes a homologue to baculoviral p74, an envelope-associated viral structural protein, has been identified and sequenced on the genome of choristoneura fumiferana granulovirus (chfugv). a part of the chfugv p74 gene was located on an 8.9 kb bamhi subgenomic fragment using different sets of degenerated primers. these were designed using the results of the protein sequencing of a major 74 kda structural protein that is associated with the occlusion-derived virus (odv). the gene has a ... | 2003 | 14536031 |
| transgenic tobacco and apple plants expressing biotin-binding proteins are resistant to two cosmopolitan insect pests, potato tuber moth and lightbrown apple moth, respectively. | tobacco (nicotiana tabacum cv. samsun) and apple (malus x domestica cv. royal gala) plants expressing avidin or strepavidin were produced using agrobacterium tumefaciens-mediated transformation. elisa assays showed that avidin expression ranged from 3.1 to 4.6 microm in tobacco and from 1.9 to 11.2 microm in apple and streptavidin expression ranged from 11.4 to 24.5 microm in tobacco and from 0.4 to 14.6 microm in apple. expressed at these levels, both biotin-binding proteins conferred a high le ... | 2003 | 14713196 |
| field and storage testing bt potatoes for resistance to potato tuberworm (lepidoptera: gelichiidae). | potato tuberworm, phthorimaea operculella (zeller), is the most serious insect pest of potatoes worldwide. the introduction of the bacillus thuringiensis (bt) toxin gene through genetic engineering offers host plant resistance for the management of potato tuberworm. we report on the field and storage studies to evaluate bt-cry5 potato lines for resistance to potato tuberworm in egypt under natural infestations and their agronomic performance in both egypt and michigan. from 1997 to 2001, field e ... | 2004 | 15384356 |
| identification, characterization and phylogenic analysis of conserved genes within the odvp-6e/odv-e56 gene region of choristoneura fumiferana granulovirus. | the genes that are located within the odvp-6e/odv-e56 region of the choristoneura fumiferana granulovirus (chfugv) were identified by sequencing the 11 kb bamhi restriction fragment on the chfugv genome. the global gc content that was calculated from the data obtained from this genomic region was 34.96%. the open-reading frames (orfs), located within the odvp-6e/odv-e56 region, are presented and compared to the equivalent orfs that are located at the same region in other gvs. this region is comp ... | 2004 | 15469697 |
| promoter and piggybac activities within embryos of the potato tuber moth, phthorimaea operculella, zeller (lepidoptera: gelechiidae). | potato production in tropical and subtropical countries suffers from damage caused by the potato tuber moth (ptm), phthorimaea operculella. the aim of this research was the development of the components required for a germline transformation system for the ptm. we tested three components that are critical to genetic transformation systems for insects: promoter activity, marker gene expression, and transposable element function. we compared the transcriptional activities of five different promote ... | 2004 | 15527988 |
| identification, characterization and phylogenic analysis of conserved genes within the p74 gene region of choristoneura fumiferana granulovirus genome. | the genes located within the p74 gene region of the choristoneura fumiferana granulovirus (chfugv) were identified by sequencing an 8.9 kb bamhi restriction fragment on the chfugv genome. the global guanine-cytosine (gc) content of this region of the genome was 33.02%. this paper presents the orfs within the p74 gene region along with their transcriptional orientations. this region contains a total of 15 open reading frames (orfs). among those, 8 orfs were found to be homologues to the baculovir ... | 2004 | 15607029 |
| isolation and toxicity of bacillus thuringiensis from potato-growing areas in bolivia. | bacillus thuringiensis was isolated from 116 samples collected in high altitude potato-growing areas in bolivia. in these regions, main potato pests are the potato tuberworm phthorimaea operculella, and the andean weevils premnotrypes latithorax and rhigopsidius tucumanus. b. thuringiensis was found in 60% of the samples. the main percentage of samples with b. thuringiensis was found in larvae of r. tucumanus (78%). bioassays were performed with 112 isolates. none resulted toxic to either larvae ... | 2005 | 15707864 |
| characterization of a newly established potato tuber moth (phthorimaea operculella zeller) cell line. | potato tuber moth (ptm), phthorimaea operculella zeller is a widely distributed, devastating pest of potatoes attacking the foliage and infest the tubers in both field and store causing serious economic damage. as application of ptm granulovirus (ptm-gv) has shown significant reduction in damage, attempts were made to develop a new cell line from this insect to grow ptm-gv for use as a biopesticide. | 2005 | 15802757 |
| evaluation of transgenic approaches for controlling tuber moth in potatoes. | a wide spectrum of strategies to genetically engineer potato plants resistant to potato tuber moth, phthorimaea operculella (zeller), have been investigated. the potato cv iwa was transformed with a range of genes under the transcriptional control of the camv 35s promoter using agrobacterium-mediated gene transfer. the transferred genes encode protease inhibitors (spleen inhibitor and alpha1-antitrypsin inhibitor), biotin-binding proteins (avidin and streptavidin) and cry proteins (crylac9, cry1 ... | 2005 | 16628898 |
| expression of the cry9aa2 b.t. gene in tobacco chloroplasts confers resistance to potato tuber moth. | we report here the control of potato tuber moth (phthorimaea operculella) by incorporating a truncated bacillus thuringiensis cry9aa2 gene in the plastid genome. plasmids pskc84 and pskc85 are derivatives of a new polycistronic plastid transformation vector, pprv312l, that carries spectinomycin resistance (aada) as a selective marker and targets insertions in the trni-trna intergenic region. the cry9aa2 n-terminal region (82.1 kda; 734 amino acids) was expressed in a cassette, which consists of ... | 2006 | 16906448 |
| [trichogramma species (hymenoptera: trichogrammatidae) parasitoids of lepidopteran eggs in lara state, venezuela]. | this work was conducted to determine the occurrence of trichogramma wasp species in lara state, venezuela. lepidopteran egg samples collected from various crop leaves were observed daily under laboratory conditions for emergence of trichogramma species adult wasps. trichogramma were also obtained from traps containing eggs from the hosts sitotroga cerealella (olivier) (lepidoptera: gelechiidae) and spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae). in the laboratory, specimens of tric ... | 2007 | 17934619 |
| temperature as a key driver of ecological sorting among invasive pest species in the tropical andes. | invasive species are a major threat to the sustainable provision of ecosystem products and services, both in natural and agricultural ecosystems. to understand the spatial arrangement of species successively introduced into the same ecosystem, we examined the tolerance to temperature and analyzed the field distribution of three potato tuber moths (ptm, lepidoptera: gelechiidae), that were introduced in ecuador since the 1980s. we studied physiological responses to constant temperatures of the th ... | 2008 | 18839773 |
| evaluation of a granulovirus (pogv) and bacillus thuringiensis subsp. kurstaki for control of the potato tuberworm (lepidoptera: gelechiidae) in stored tubers. | liquid suspensions and dry formulations of a granulovirus (family baculoviridae, genus granulovirus, pogv) derived from infected larvae and the bacterium bacillus thuringiensis subsp. kurstaki (berliner) (btk) were evaluated for control of the potato tuberworm, phthorimaea operculella (zeller) (lepidoptera: gelechiidae), in stored tubers. laboratory bioassays at 25 degrees c showed that both pogv and a wettable powder (wp) formulation of btk incorporated with carriers (water, talc, sand, diatoma ... | 2008 | 18950035 |
| green-tissue-specific, c(4)-pepc-promoter-driven expression of cry1ab makes transgenic potato plants resistant to tuber moth (phthorimaea operculella, zeller). | an important strategy for obtaining a safer transgenic plant may be the use of a spatial- or tissue-specific promoter, instead of a constitutive one. in this study, we have used a light-inducible maize pepc promoter to regulate the cry1ab gene, aiming to produce transgenic potatoes that are resistant to potato tuber moth (ptm) (phthorimaea operculella, zeller). out of 60 regenerated lines having normal phenotypes, 55 lines were pcr-positive for both the cry1ab and nptii genes. southern analysis ... | 2009 | 19882159 |
| expression of various biotin-binding proteins in transgenic tobacco confers resistance to potato tuber moth, phthorimaea operculella (zeller) (fam. gelechiidae). | the high affinity biotin-binding proteins (bbps) avidin and streptavidin are established insecticidal agents, effective against a range of insect pests. earlier work showed that, when expressed in planta, full length avidin and a truncated form of streptavidin are highly insecticidal. more recently, a wide range of bbps, found in diverse organisms or engineered for various biotechnological applications have been reported. however, their effectiveness as plant-based insecticides has not been esta ... | 2010 | 20217475 |
| population structure of phthorimaea operculella (lepidoptera: gelechiidae) in the united states. | the potato tuberworm, phthorimaea operculella zeller, is one of the most damaging pests of potatoes worldwide. although tuberworm was recorded in california as early as 1856, it was first reported in damaging numbers in oregon and washington in the early 2000s. the objective of this study was to provide baseline information on the population structure of potato tuberworm in the united states. potato tuberworm adults were collected from potato fields in the major production regions in the united ... | 2010 | 20550820 |
| regeneration of multiple shoots from transgenic potato events facilitates the recovery of phenotypically normal lines: assessing a cry9aa2 gene conferring insect resistance. | the recovery of high performing transgenic lines in clonal crops is limited by the occurrence of somaclonal variation during the tissue culture phase of transformation. this is usually circumvented by developing large populations of transgenic lines, each derived from the first shoot to regenerate from each transformation event. this study investigates a new strategy of assessing multiple shoots independently regenerated from different transformed cell colonies of potato (solanum tuberosum l.). | 2011 | 21995716 |
| biological effects of some natural and chemical compounds on the potato tuber moth, phthorimaea operculella zell. (lepidoptera:gelechiidae). | the olfactory reaction of larvae and moths was investigated towards 18 oils (6 natural oils and 12 commercial chemical oils). some of these oils such as peppermint and camphor (natural oils) and eugenol and camphene (commercial oils) were repellent to both larvae and moths. other oils such as strawberry and d-limonene were attractive to both larvae and moths. some of the repellent oils were, therefore, tested for their effect on certain biological aspects of the insects. eugenol and peppermint o ... | 0 | 23961036 |
| segregation and efficacy of the cry1ia1 gene for control of potato tuberworm in four populations of cultivated potato. | the potato (solanum tuberosum l.) cultivar 'spuntag2' contains a single copy of the bacillus thuringiensis (bt) cry1ia1 gene and controls potato tuberworm (phthorimaea operculella zeller, lepidoptera: gelechiidae). two potato cultivars and two breeding lines were crossed with spuntag2 creating four populations used to study cry1ia1 segregation and efficacy. the cry1ia1 gene segregated in each of the four populations with a 1:1 ratio. all progeny that were polymerase chain reaction positive for t ... | 2013 | 23786096 |
| betabaculovirus f proteins showed different efficiencies when rescuing the infectivity of gp64-null autographa californica nucleopolyhedrovirus. | the agrotis segetum granulovirus (agsegv) f protein was previously identified as the first betabaculovirus f protein with functional homology to autographa californica nucleopolyhedrovirus (acmnpv) gp64. in the current study, f proteins from xestia c-nigrum granulovirus (xecngv), cydia pomonella granulovirus (cpgv), phthorimaea operculella granulovirus (phopgv), choristoneura occidentalis granulovirus (chocgv) and plutella xylostella gv (plxygv) were studied for their ability to rescue the infec ... | 2013 | 23245471 |
| the comparative analysis of complete genome sequences from two south african betabaculoviruses: phthorimaea operculella granulovirus and plutella xylostella granulovirus. | the complete genomes of two novel south african betabaculovirus isolates, namely phthorimaea operculella granulovirus (phopgv-sa) and plutella xylostella granulovirus (plxygv-sa), were sequenced and compared to the respective reference isolates phopgv-1346 and plxygv-k1. for both isolates, the genome size and guanine-cytosine (gc) content were similar to those of the respective reference genomes. however, numerous-single nucleotide polymorphisms (snps) and several insertions/deletions were obser ... | 2016 | 27456426 |
| the isolation and genetic characterisation of a south african strain of phthorimaea operculella granulovirus, phopgv-sa. | the phthorimaea operculella granulovirus (phopgv) is considered a promising biopesticide that can be incorporated into integrated pest management programmes for sustainable control of the potato tuber moth, phthorimaea operculella (zeller) (lepidoptera: gelechiidae), a major pest of solanaceous crops in sub-tropical and tropical regions worldwide. several phopgv isolates recovered from geographically different insect populations have been genetically characterised, and the full genome of the tun ... | 2014 | 24503224 |
| development of a viral biopesticide for the control of the guatemala potato tuber moth tecia solanivora. | the guatemala potato tuber moth tecia solanivora (povolny) (lep. gelechiidae) is an invasive species from mesoamerica that has considerably extended its distribution area in recent decades. while this species is considered to be a major potato pest in venezuela, colombia, and ecuador, currently no specific control methods are available for farmers. to address this issue we developed a biopesticide formulation to be used in integrated pest management of t. solanivora, following three steps. first ... | 2013 | 23232473 |
| experimental mixtures of phthorimaea operculella granulovirus isolates provide high biological efficacy on both phthorimaea operculella and tecia solanivora (lepidoptera: gelechiidae). | the guatemalan potato moth tecia solanivora (povolny) recently invaded part of south america, colonizing zones where phthorimaea operculella (zeller), another potato moth species belonging to the same group, was previously established. t. solanivora is now the major insect pest of potato in this area encompassing venezuela, colombia and ecuador. p. operculella granulovirus (phopgv) (betabaculovirus) is a biocontrol agent to be considered for the simultaneous management of these two potato pests, ... | 2012 | 22575733 |
| genetic and biological analysis of colombian phthorimaea operculella granulovirus isolated from tecia solanivora (lepidoptera: gelechiidae). | tecia solanivora (lepidoptera: gelechiidae) is an invasive potato pest of the north of south america that recently colonized zones where phthorimaea operculella (lepidoptera: gelechiidae), a taxonomically related insect, was established. nowadays, both species can be found in most areas in different proportions. the phthorimaea operculella granulovirus (phopgv) was found to efficiently control p. operculella and was used as a biopesticide in storage conditions. however, no appropriate biological ... | 2010 | 20870793 |
| establishment and characterization of spodoptera littoralis cell line (sl 96), adapted at 19 degrees c permissive for different baculoviruses. | cells from ovarioule tissues of the egyptian cotton leaf worm, spodoptera littoralis (sl 96) were established, identified and characterized comparing with other lepidopteran cell lines using isozymes, karyotyping, rapd pcr, and polyacrylamide gel electrophoresis polypeptide page profile. comparison of the number of chromosomes and page profiles; also the isozymes profiles demonstrate the possibility to distinguish the cell lines by those analyses and having a distinguishable finger print for eve ... | 2005 | 16333885 |
| detection of phthorimaea operculella granulovirus in vivo and in vitro by a specific dna probe. | in analyzing populations of non-infected potato tuber moth (ptm) phthorimaea operculella, using a total dna probe from phthorimaea operculella granulovirus (phopgv), false positive reactions were obtained indicating homology between cellular and viral dnas. using a cloned 2.1 kbp fragment of phopgv dna, a specific digoxigenin-labeled probe was developed. this fragment did not show homology using both dot and southern blot hybridization with either the genome of the larvae or genomes of the cell ... | 1999 | 10757228 |
| genetic heterogeneity of phthorimaea operculella granulovirus: restriction analysis of wild-type isolates and clones obtained in vitro. | genetic heterogeneity of a wild-type granulovirus (tunisia isolate) of the potato tuber moth phthorimaea operculella (phthorimaea operculella granulovirus, phop gv) has been studied. the heterogeneity was indicated by the presence of several submolar fragments in the profiles obtained by use of several restriction endonucleases. it was also demonstrated by variations in the restriction profile of the wild-type tunisia isolate that had underwent since 1991 in our laboratory numerous passages in v ... | 1998 | 9645238 |
| the origins of replication of granuloviruses. | the genomes of eight granuloviruses (gvs), have been analyzed for the presence of homologous regions (hrs) that may act as origins of replication. thirteen 74-76-bp palindromes within 11 hrs have previously been identified in the cydia pomonella gv (cpgv) genome and found to replicate in an infection-dependent dna replication assay. we report a further palindrome within one of the hrs, which was found to replicate, bringing the total to 14 palindromes. we also report imperfect palindromes, with ... | 2008 | 18612587 |
| insecticidal activity of the granulosis virus in combination with neem products and talc powder against the potato tuberworm phthorimaea operculella (zeller) (lepidoptera: gelechiidae). | the potato tuberworm phthorimaea operculella (zeller) is an important agricultural pest that causes significant economic losses to potato growers worldwide. the addition of an effective method of biological control for the potato tuberworm is greatly needed, and is currently unavailable in brazil. the granulosis virus (baculoviridae) is a promising biological control agent to protect post-harvest potatoes and in storage from the potato tuberworm. however, the control measure must be economically ... | 2012 | 23950047 |
| seasonal population dynamics of three potato pests in washington state. | pest phenology models allow producers to anticipate pest outbreaks and deploy integrated pest management (ipm) strategies. phenology models are particularly useful for cropping systems with multiple economically damaging pests throughout a season. potato (solanum tuberosum l.) crops of washington state, usa, are attacked by many insect pests including the potato tuberworm (phthorimaea operculella zeller), the beet leafhopper (circulifer tenellus baker), and the green peach aphid (myzus persicae ... | 2016 | 27271946 |
| efficiency of guva and lemon grass fine dusts on the potato tuber moth phthorimaea operculella (zeller) (lepidoptera: gelechiidae). | the present study was initiated to elucidate the effect of potato tubers treatment using fine dusts of psidium guajava, cymbopogon citratus, talc powder and their progressive dilutions(w/w) on the fitness components of raised generations of the potato tuber moth (ptm), phthorimaea operculella. results showed a direct as well as cumulative delayed effect of the tested plants fine dusts, either alone or admixed with talc powder in progressive dilutions from 5% up to 50% w/w on the viability of dev ... | 2012 | 23885440 |