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[absence of mutagenic effect of pseudomonas syringae pv. atrofaciens 9400 and pantoea agglomerans p324 culture liquids].the mutagenic activity of the culture liquids of phytopathogenic strain pseudomonas syringae pv. atrofaciens 9400 and epiphytic strain pantoea agglomerans p324 was studied in the ames test and allium cepa-test. in pro- and eucariotic test-systems no effect of the culture liquids of these bacteria on spontaneous mutations of salmonella typhimurium ta98 and ta100 or chromosome aberrations in the cells of allium cepa root apical meristem was found.201020812509
[composition, structure, and biological properties of lipopolysaccharides from different strains of pseudomonas syringae pv. atrofaciens].the composition, structure, and certain biological properties of lipopolysaccharides (lps) isolated from six strains of bacteria pseudomonas syringae pv. atrofaciens pathogenic for grain-crops (wheat, rye) are presented. the lps-protein complexes were isolated by a sparing procedure (extraction from microbial cells with a weak salt solution). they reacted with the homologous o sera and contained one to three antigenic determinants. against the cells of warm-blooded animals (mice, humans) they ex ...200718297868
[interaction of pantoea agglomerans with the agent of basal bacteriosis of wheat].interactions between the agent of basal bacteriosis pseudomonas syringae pv. atrofaciens and epiphyte pantoea agglomerans have been studied in the laboratory and field conditions. it is shown the p. agglomerans affects the infectious process caused by p. s. pv. atrofaciens that leads to intensification of symptoms of the disease development. the epiphyte cells accumulation predominates under mutual artificial infection in the infected tissue. the higher competitive ability of the epiphyte p. agg ...200515765881
[serological heterogeneity of pseudomonas syringae pv. atrofaciens strains and their ecological niches].the paper deals with a comparative analysis of the serological and ecological properties of pseudomonas syringae pv. atrofaciens strains from the collections of microbial cultures at the malkov institute for plant genetic resources and zabolotny institute of microbiology and virology. all of the strains from the bulgarian collection, except for one, fall into five serogroups (ii through vi) of the classification system of pastushenko and simonovich. the p. syringae pv. atrofaciens strains isolat ...200314768551
[effect of the lipopolysaccharide-protein complex of pseudomonas syringae pv. atrofaciens on the process of tumor formation caused by agrobacterium tumefaciens].it has been found that the agent of bacterial spotting of rye p. syringae pv. atrofaciens 8281 has no antagonistic action on the strains 9052 and 9054 of agrobacterium tumefaciens--the agent of crown gall tumor. lipopolysaccharide-protein complex of p. syringae pv. atrofaciens 8281 when added to the culture medium does not affect the growth and development of a. tumefaciens bacteria. it has been experimentally established that the lipopolysaccharide-protein complex of p. syringae pv. atrofaciens ...200312945188
characterization of the lipopolysaccharide and structure of the o-specific polysaccharide of the bacterium pseudomonas syringae pv. atrofaciens imv 948.lipopolysaccharide (lps) was isolated from the phytopathogenic bacterium pseudomonas syringae pv. atrofaciens imv 948 by mild extraction of the microbial cells with saline, and the properties, composition, and structure of the lps were studied. the lps showed low toxicity in d- galactosamine-sensitized mice and low biological activity in plants. structural components of lps--lipid a, core oligosaccharide, and o-specific polysaccharide (ops)--were obtained by mild acid degradation and characteriz ...200111403642
immunochemical characterization and taxonomic evaluation of the o polysaccharides of the lipopolysaccharides of pseudomonas syringae serogroup o1 strains.the o polysaccharide (ops) of the lipopolysaccharide (lps) of pseudomonas syringae pv. atrofaciens imv 7836 and some other strains that are classified in serogroup o1 was shown to be a novel linear alpha-d-rhamnan with the tetrasaccharide o repeat -->3)-alpha-d-rhap-(1-->3)-alpha-d-rhap-(1-->2)-alpha-d-r hap-(1-->2)- alpha-d-rhap-(1--> (chemotype 1a). the same alpha-d-rhamnan serves as the backbone in branched opss with lateral (alpha1-->3)-linked d-rhap, (beta1-->4)-linked d-glcpnac, and (alpha ...199910559159
[antigenic bacterial polysaccharides. 28. the structure of the o-specific lipopolysaccharide chain of pseudomonas syringae pv. atrofaciens k-1025 and pseudomonas holci 90a (serogroup ii)].lipopolysaccharides of serologically related strains of pseudomonas syringae pv. atrofaciens k-1025 and pseudomonas holci 90a possess the identical o-specific polysaccharide chains, representing a homopolymer of d-rhamnose. on the basis of methylation, partial and complete smith degradation, and analysis by 1h- and 13c-nmr-spectroscopy, it was concluded that the repeating unit of the polysaccharide is a branched pentasaccharide of the following structure: (formula; see text)19882454627
[antigenic polysaccharides of bacteria. 27. structure of the o-specific polysaccharide chain of lipopolysaccharides from pseudomonas syringae pv. atrofaciens 2399, phaesolica 120a and pseudomonas holci 8299 belonging to serotype vi].lipopolysaccharides from pseudomonas syringae pvs atrofaciens 2399. phaseolicola 120a and pseudomonas holci 8299, belonging to serogroup vi. possess an identical polysaccharide chain composed of d-rhamnose and d-fucose. on the hasis of methylation, partial acid hydrolysis, 1h- and 13c-nmr data, it was concluded that the backbone of the polysaccharide represents d-rhamnan built up of tetrasaccharide repeating units and alpha-d-fucofuranose residues are attached to the backbone as the monosacchari ...19882454626
[isolation and partial characteristics of biopolymers in the outer membrane of pseudomonas syringae].conditions are developed for obtaining extracellular biopolymers (ebp), water (w), salt-edta (se) and salt-detergent (sd) extracts of pseudomonas syringae pv. atrofaciens imv k-1025. the fraction of unsoluble lps is effectively precipitated from samples by ultracentrifugation (uc). the fractions enriched by proteins and soluble lps are salted-out from the uc supernatants by ammonium sulphate, that is confirmed by the chemical analysis, electrophoresis in the paag-nds and in elisa with the monocl ...19902368185
[the polysaccharide glycocalyx of pseudomonas syringae pv. atrofaciens].it was shown by the method of electron microscopy that cells of virulent strain pseudomonas syringae rv. atrofaciens 4394 have extracellular, probably, polysaccharide glycocalix. it consists of acid components giving positive cytochemical reaction with ruthenium red, a specific reagent to polyanions.19902352506
[an analysis of the molecular forms of the lipopolysaccharide from pseudomonas syringae pv. atrofaciens imv k-1025].water extract and salt-edta extract of pseudomonas syringae, pv. atrofaciens cells were fractionated by ultracentrifugation with following salting out of ultracentrifugal supernatant by ammonium sulphate at 55% saturation (ph 4.5). the composition and distribution of lps molecular forms were studied in the obtained fractions by means of electrophoresis in 10% polyacrylamide gel with 1% sodium dodecylsulphate when staining gels by silver nitrate and cumassi. it is shown that ultracentrifugal supe ...19912067421
[mechanism of antimutagenic activity of pseudomonas syringae lipopolysaccharides].the antimutagenic activity of lipopolysaccharides of phytopathogenic bacteria,which are the agents of grain crops diseases, has been investigated using bacterial test-system with salmonella typhimurium. it has been established that the greatest antimutagen activity of pseudomonas syringae pv. atrofaciens and p. syringae pv. coronafaciens lipopolysaccharides was,if they and bichromate potassium be simultaneously added to cells test-strains of s. typhimurium. they decreased to a less degree quanti ...200516018214
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