| marinilactibacillus psychrotolerans gen. nov., sp. nov., a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of japan. | a novel marine lactic acid rod bacterium has been described for eight strains isolated from living and decomposing marine organisms collected from temperate and subtropical areas of japan. the isolates were gram-positive, catalase-negative, non-sporulating and motile with peritrichous flagella. they were slightly halophilic, highly halotolerant and alkaliphilic; the optimum nacl concentration for growth was 2.0-3.75% (w/v) with a range from 0 to 17.0-20.5% (depending on the strain); the optimum ... | 2003 | 12807191 |
| temporal stability and biodiversity of two complex antilisterial cheese-ripening microbial consortia. | the temporal stability and diversity of bacterial species composition as well as the antilisterial potential of two different, complex, and undefined microbial consortia from red-smear soft cheeses were investigated. samples were collected twice, at 6-month intervals, from each of two food producers, and a total of 400 bacterial isolates were identified by fourier-transform infrared spectroscopy and 16s ribosomal dna sequence analysis. coryneform bacteria represented the majority of the isolates ... | 2003 | 12839776 |
| alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye. | a psychrotolerant, obligately alkaliphilic bacterium, idr2-2(t), which is able to reduce indigo dye was isolated from a fermented polygonum indigo (polygonum tinctorium lour.) produced in date, hokkaido, using a traditional japanese method. the isolate grew at ph 9-12 but not at ph 7-8. it was a gram-positive, facultatively anaerobic, straight rod-shaped bacterium with peritrichous flagella. the isolate grew in 0-17 % (w/v) nacl but not at nacl concentrations higher than 18 % (w/v). its major ce ... | 2004 | 15545487 |
| marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the nankai trough. | a piezotolerant, mesophilic, marine lactic acid bacterium (strain lt20t) was isolated from a deep sub-seafloor sediment core collected at nankai trough, off the coast of japan. cells were gram-positive, rod-shaped, non-sporulating and non-motile. the nacl concentration range for growth was 0-120 g l(-1), with the optimum at 10-20 g l(-1). the temperature range for growth at ph 7.0 was 4-50 degrees c, with the optimum at 37-40 degrees c. the optimum ph for growth was 7.0-8.0. the optimum pressure ... | 2005 | 15653899 |
| atopococcus tabaci gen. nov., sp. nov., a novel gram-positive, catalase-negative, coccus-shaped bacterium isolated from tobacco. | a novel gram-positive, aerobic, catalase-negative, coccus-shaped organism originating from tobacco was characterized using phenotypic and molecular taxonomic methods. the organism contained a cell wall murein based on l-lysine (variation a4alpha, type l-lysine-l-glutamic acid), synthesized long-chain cellular fatty acids of the straight-chain saturated and monounsaturated types (with c(16:1)omega9, c(16:0) and c(18:1)omega9 predominating) and possessed a dna g+c content of 46 mol%. based on morp ... | 2005 | 16014503 |
| presence of halophilic and alkaliphilic lactic acid bacteria in various cheeses. | we sought to confirm the presence of halophilic and alkaliphilic lactic acid bacteria (halab) of marine origin in cheeses and thus contribute to the understanding of the roles of lab flora in cheese ripening. | 2007 | 17309509 |
| culturable prokaryotic diversity of deep, gas hydrate sediments: first use of a continuous high-pressure, anaerobic, enrichment and isolation system for subseafloor sediments (deepisobug). | deep subseafloor sediments may contain depressurization-sensitive, anaerobic, piezophilic prokaryotes. to test this we developed the deepisobug system, which when coupled with the hyacinth pressure-retaining drilling and core storage system and the press core cutting and processing system, enables deep sediments to be handled without depressurization (up to 25 mpa) and anaerobic prokaryotic enrichments and isolation to be conducted up to 100 mpa. here, we describe the system and its first use wi ... | 2009 | 19694787 |
| molecular monitoring of culturable bacteria from deep-sea sediment of the nankai trough, leg 190 ocean drilling program. | culturable bacteria were detected in deep-sea sediment samples collected from the nankai trough site 1173 (ocean drilling program, odp, leg 190) at 4.15 m below the seafloor with 4791 m of overlying water. in this deep ocean near surface sediment, mainly fermentative heterotrophs, autotrophic acetogens and sulfate-reducing bacteria were enriched by using two different non-selective enrichment culture media. culturable bacterial population shifts within the deep marine sediment enrichments were m ... | 2004 | 19712305 |
| population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis. | surface contamination of smear cheese by listeria spp. is of major concern for the industry. complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties in situ were investigated by tem ... | 2010 | 20222967 |
| 16srrna and enzymatic diversity of culturable bacteria from the sediments of oxygen minimum zone in the arabian sea. | sediment underlying the oxygen minimum zone of the eastern arabian sea is rich in organic matter. bacteria in this sediment-water interface are of great ecological importance as they are responsible for decomposing, mineralizing and subsequent recycling of organic matter. this study has for the first time addressed the phylogenetic and functional description of culturable bacteria of this region. genotypic characterization of the isolates using amplified rdna restriction analysis (ardra) followe ... | 2010 | 20229220 |
| isolation and characterisation of bacteria from the haloalkaline lake elmenteita, kenya. | culture-independent studies show that soda lake environments harbour diverse groups of bacteria and archaea. in this study different enrichment and isolation media were used in an attempt to isolate novel groups of bacteria from lake elmenteita. different media were prepared using filter-sterilised water from the lake. the isolates recovered were purified on tryptic soy agar supplemented with 1% sodium carbonate and 4% sodium chloride. phylogenetic analysis of 181 partial 16s rrna gene sequences ... | 2010 | 20401502 |
| facultative anaerobic halophilic and alkaliphilic bacteria isolated from a natural smear ecosystem inhibit listeria growth in early ripening stages. | in vitro and in situ anti-listerial properties of 3 strains of facultative anaerobic halophilic and alkaliphilic (faha) species, i.e. alkalibacterium kapii alk 6, marinilactibacillus psychrotolerans alk 9 and facklamia tabacinasalis alk 1, were investigated. the 3 strains were isolated from a smear ecosystem originating from a commercial raclette type cheese and exhibiting strong anti-listerial activity in situ on cheese surface. in a first step, strains were tested in vitro for production of an ... | 2011 | 21440945 |
| identification and characterisation of halotolerant bacteria in spoiled dry-cured hams. | twenty bacterial strains isolated from italian dry-cured hams affected by the so-called 'vein defect', were gram positive, catalase and oxidase negative non-spore-forming rods. twelve strains were identified by molecular characterisation as marinilactibacillus psychrotolerans. these strains were demonstrated to survive at high salt concentrations (up to 25% w/w, with growth up to 12% w/w), low temperatures (0-3°c) and a ph range (6-7), which is encountered within the leg arterial vein. if strain ... | 2005 | 22063480 |