| [apoptosis resistance and its reversal in harringtonine resistant cell line]. | harringtonine (ht), a domestic antitumor drug extracted from cephalotaxus hainanensis li showed high chemotherapeutic efficacy on human acute granulocytic leukemia and acute myelocytic leukemia in clinics. apoptosis of hl-60 cells can be induced by ht effectively; but for cells resistant to harringtonine, apoptosis can not be induced, even if the drug (ht) concentration is over 100 times of ic50 value. although apoptosis occurred when its multidrugs resistance had been reversed by verapamil, com ... | 1994 | 7709741 |
| [isoharringtonine induces apoptosis in human leukemia hl-60 cell]. | harringtonine (ht), homoharringtonine (hht) and isoharringtonine (iht) are cephalotaxine alkaloids with anticancer activities which were isolated from cephalotaxus hainanensis indigenous to china. since the 1970s, ht and hht have been developed as effective anticancer drugs in china and have been used widely in the treatment of acute nonlymphoid leukemia and chronic granulocyte leukemia. although iht has the advantage of low toxicity, it has not been developed as an anticancer drug because of it ... | 1998 | 12016908 |
| molecular modes of action of cephalotaxine and homoharringtonine from the coniferous tree cephalotaxus hainanensis in human tumor cell lines. | homoharringtonine (hht) is an ester of cephalotaxine (cet), both of which derive from the chinese coniferous tree cephalotaxus hainanensis. hht inhibited tumor cell growth at molar ranges comparable to established cytostatic drugs, whereas cet was 3-4 orders of magnitude less active. inhibition concentration 50% (ic50) values of cet and hht were significantly correlated to doxorubicin, vincristine, methotrexate, cisplatin, or camptothecin in 55 cell lines of the developmental therapeutics progra ... | 2003 | 12616342 |
| a new 5-acyl-2-methylpyrrole from the endophytic fungus s20 of cephalotaxus hainanensis. | a new 5-acyl-2-methylpyrrole, isolated from the endophytic fungus s20 of cephalotaxus hainanensis, was elucidated as 1-(5-methyl-1h-pyrrol-2-yl)-2-((2s*,3r*)-3-((e)-prop-1-enyl)oxiran-2-yl)ethanone (1) on the basis of spectroscopic evidence, including 1d- and 2d-nmr (hmqc, 1h-1h cosy, hmbc and roesy) and ms analysis. compound 1 showed inhibitory effects on staphylococcus aureus and methicillin-resistant s. aureus by the filter paper disc agar diffusion method. | 2009 | 19967980 |
| metabolites from endophytic fungus s20 of cephalotaxus hainanensis. | three new c-methylated acetogenins, (2e,6z)-9,10-dihydroxy-4-hydroxymethyl-2,6-decadiene (1), (2e,6z)-8,9,10-trihydroxy-4-hydroxymethyl-2,6-decadiene (2), and (2e,6z)-9-hydroxy-4-hydroxymethyl-2,6-nonadiene (3), together with two known compounds, p-hydroxybenzyl alcohol (4) and indolyl-3-carboxylic acid (5), were isolated from endophytic fungus s20 of cephalotaxus hainanensis li. their structures were determined based on hr-esi-ms and spectroscopic techniques (ir, uv, 1d, and 2d nmr). compound 5 ... | 2009 | 20183311 |
| homoharringtonine production by endophytic fungus isolated from cephalotaxus hainanensis li. | homoharringtonine (hht), a natural plant alkaloid derived from cephalotaxus, has demonstrated to have a broad antitumor activity and efficacy in treating human chronic myeloid leukemia. an alternative source is required to substitute for the slow-growing and scarce cephalotaxus to meet the increasing demand of the drug market. the objective of this study was to screen hht-producing endophytic fungi from cephalotaxus hainanensis li. by screening 213 fungal isolates obtained from the bark parts of ... | 2016 | 27263005 |
| diversity and antimicrobial activity of endophytic fungi isolated from cephalotaxus hainanensis li, a well-known medicinal plant in china. | about 1051 endophytic fungi were isolated from leaves, branches, barks and stems of cephalotaxus hainanensis li from four sites in hainan, china. the fungi were identified as 21 genera by morphology and its sequences. one dominant species was phomopsis quercella in hainan tropical botanical garden and bawangling nature reserve, with relative frequency of 42·06 and 34·88% respectively. another dominant species was colletotrichum boninense in wuzhishan and jianfengling nature reserves, with relati ... | 2015 | 26280451 |
| antimicrobial and antitumor activity and diversity of endophytic fungi from traditional chinese medicinal plant cephalotaxus hainanensis li. | endophytes from cephalotaxus hainanensis li, an important source of anti-leukemia drugs, have not been widely explored. in this study, 265 endophytic fungal isolates from c. hainanensis li were screened for antimicrobial activities against tilapia, banana, rice, and rape and for antitumor activities against human leukemia cell lines (k562, nb4, and hl-60). diversity was also analyzed. the results showed that 17.7% of the endophytic fungi had antimicrobial activities against at least three differ ... | 2016 | 27323030 |
| aromatic compounds from endophytic fungus colletotrichum sp. l10 of cephalotaxus hainanensis li. | six aromatic compounds were isolated from the endophytic fungus colletotrichum sp. of cephalotaxus hainanensis li [cephalotaxus mannii hook. f.]. their structures were determined on the basis of chemical and spectroscopic methods. the compound 2 was the enantiomer of compound 1. the compound 4 was the epimeride of the compound 3. the compounds 1, 2, 4 and 5 were evaluated for cytostatic activity against hl-60 and pc-3 cells. | 2012 | 22417867 |
| two new compounds from the endophytic fungus colletotrichum sp. l10 of cephalotaxus hainanensis. | two new compounds, 3-(4-methoxy-3-methyl-2-oxo-2h-pyran-6-yl)butyl pyroglutamate (1) and 2,2'-(1,1'-methylenebis(1h-indole-3,1-diyl))diethanol (2), were isolated from the endophytic fungus of cephalotaxus hainanensis. their structures were determined by means of chemical and spectroscopic analysis. | 2011 | 21985614 |
| de novo characterization of a cephalotaxus hainanensis transcriptome and genes related to paclitaxel biosynthesis. | cephalotaxus hainanensis, an endangered plant, is known to contain several metabolites with anti-cancer activity. despite its clinical impact, the alkaloid metabolism of this species has remained largely uncharacterized. the potential of cephalotaxus for metabolic engineering of medically interesting compounds has, so far, not been exploited, due to the almost complete lack of molecular information. we have therefore performed a high throughput rna-seq analysis and assembled the transcriptome de ... | 2014 | 25203398 |
| two new sesquiterpenes from endophytic fungus s49 of cephalotaxus hainanensis. | two new sesquiterpenes, 10,11,12-guaianetriol (1) and 1,10,11,12-guaianetetrol (2), were isolated from endophytic fungus s49 of cephalotaxus hainanensis li. their structures were determined based on hr-esi-ms and spectroscopic techniques (1d and 2d nmr). | 2010 | 20628937 |
| three new compounds from endophytic fungus l10 of cephalotaxus hainanensis. | three new compounds, 4-hydroxyphenethyl-2'-hydroxypropanoate (1), 6-(1',2'-dimethyloxiran-1'-yl)-4-methoxy-3-methyl-2h-pyran-2-one (2), 6-(1-hydroxyethyl)-4-methoxy-3-methyl-2h-pyran-2-one (3), along with the two known compounds, nectriapyrone (4) and wermopyrone (5), have been isolated from the endophytic fungus of cephalotaxus hainanensis. their structures were determined on the basis of chemical and spectroscopic methods. | 2009 | 19504381 |
| simultaneous multi-parameter observation of harringtonine-treating hl-60 cells with both two-photon and confocal laser scanning microscopy. | harringtonine (ht), a kind of anticancer drug isolated from chinese herb-cephalotaxus hainanensis li, can induce apoptosis in promyelocytic leukemia hl-60 cells. with both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe hoechst 33342, tetramethyrhodamine ethyl ester (tmre) and fluo 3-am, we simultaneously observed ht-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ... | 2001 | 18726419 |
| [induction of apoptosis by harringtonine and homoharringtonine in hl-60 cells]. | harringtonine (ht) and homoharringtonine (hht) are two alkaloids isolated from the bark of the evergreen tree cephalotaxus hainanensis li in the 1970s. they were found to have activity against murine leukemia, lewis lung carcinoma and b16 melanoma, and used as anti-leukemia drugs clinically. apoptosis is an active process of programmed cell suicide and now is believed to be an important target for tumor chemotherapy. in this report, the apoptosis inducing effect of ht and hht in hl-60 cells were ... | 1994 | 7900538 |
| [antitumor activities and mechanisms of action of harringtonine and homoharringtonine]. | the antitumor activities and the mechanisms of action of harringtonine and homoharringtonine, alkaloids isolated from cephalotaxus hainanensis li, were compared to those of vincristine. the results obtained were as follows: harringtonine and homoharringtonine significantly inhibited the growth of l1210 cells in culture. the ic50 values were similar to that of vincristine. harringtonine and homoharringtonine had little effects on changes in the dna histograms of fl cells at any concentrations, wh ... | 1984 | 6497398 |
| [the antiviral effect of hainanolide, a compound isolated from cephalotaxus hainanensis (author's transl)]. | | 1981 | 7342682 |
| [studies on the minor alkaloids of cephalotaxus hainanensis li (author's transl)]. | | 1981 | 7336935 |
| [chemical, pharmacological and clinical studies on the antitumor active principle of cephalotaxus hainanensis li (author's transl)]. | | 1979 | 298845 |