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use of cross-reactive antigens of the microsporidian glugea atherinae for the possible detection of enterocytozoon bieneusi by western blot.the microsporidia enterocytozoon bieneusi is reported in 10-30% of those infected with the human immunodeficiency virus. the parasite appears to be a cause of gastralgia, malabsorption, and diarrhea. a western blot technique using another microsporidian species, glugea atherinae, has demonstrated an antigenic similarity between this parasite and e. bieneusi. preliminary results show the variability of the antigenic profiles obtained from the sera of immunodeficient patients infected with e. bien ...19957856832
in vitro culture of glugea sp.recently the low host specificity of some microsporidians has been demonstrated and it has been indicated that many of these micro-organisms could be transmitted from invertebrates to mammals and adapt to changes in temperature. in this work, we demonstrate the first successful in vitro culture of a fish microsporidia of the genus glugea on larval cells of the mosquito aedes albopictus at 28 degrees c, and we show ultrastructural aspects of the different life cycle stages. it was impossible on s ...200312591194
long-term trends in the prevalence of cancer and other major diseases among flatfish in the southeastern north sea as indicators of changing ecosystem health.this paper analyses and discusses spatial and temporal patterns in the prevalence of major skin diseases (lymphocystis, epidermal hyperplasia/papilloma, ulcers), intestinal parasite glugea sp., and liver cancer in dab (limanda limanda) and flounder (platichthys flesus) in the dutch section of the north sea since the mid-1980s. we have attempted to relate disease prevalence trends in both species to chemical contaminant exposure and other relevant environmental factors including fish condition fa ...200919368228
Luna stain, an improved selective stain for detection of microsporidian spores in histologic sections.Microsporidia in histologic sections are most often diagnosed by observing spores in host tissues. Spores are easy to identify if they occur in large aggregates or xenomas when sections are stained with hematoxylin and eosin (H&E). However, individual spores are not frequently detected in host tissues with conventional H&E staining, particularly if spores are scattered within the tissues, areas of inflammation, or small spores in nuclei (i.e. Nucleospora salmonis). Hence, a variety of selective ...201121848126
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