| methanogen community structure in the rumens of farmed sheep, cattle and red deer fed different diets. | development of inhibitors and vaccines that mitigate rumen-derived methane by targeting methanogens relies on knowledge of the methanogens present. we investigated the composition of archaeal communities in the rumens of farmed sheep (ovis aries), cattle (bos taurus) and red deer (cervus elaphus) using denaturing gradient gel electrophoresis (dgge) to generate fingerprints of archaeal 16s rrna genes. the total archaeal communities were relatively constant across species and diets, and were less ... | 2011 | 21255054 |
| methyl coenzyme m reductase (mcra) gene based phylogenetic analysis of methanogens population in murrah buffaloes (bubalus bubalis). | the aim of the present study was to decipher the diversity of methanogens in rumen of murrah buffaloes so that effective strategies can be made in order to mitigate methane emission from these methanogens. in the present study diversity of rumen methanogens in murrah buffaloes (bubalus bubalis) from north india was evaluated by using mcr-a gene library obtained from the pooled pcr product from four animals and by using mega4 software. a total of 104 clones were examined, revealing 26 different m ... | 2011 | 21887637 |
| Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive. | To investigate the relationship between ruminal methanogen community and host enteric methane (CH(4) ) production in lactating dairy cows fed diets supplemented with an exogenous fibrolytic enzyme additive. | 2011 | 21848695 |
| Diversity analysis of methanogens in rumen of Bubalus bubalis by 16S riboprinting and sequence analysis. | The molecular diversity of rumen methanogens was investigated by 16S rDNA gene library prepared from the rumen contents obtained from Murrah buffaloes in India. Genomic DNA was isolated from adult male fistulated buffaloes and PCR conditions were set up using specific primers. Amplified product was cloned into a suitable vector, and the positive clones were selected assuming based on blue-white screening and sequenced. Positive clones were reamplified and the resulting PCR products were further ... | 2012 | 22155312 |
| molecular profiling and identification of methanogenic archaeal species from rabbit caecum. | during a comparison of 16s rdna pcr-denaturant gradient gel electrophoresis (dgge) profiles of methanogenic archaea from rumen fluid, rabbit caecum and pig feces, a unique band common to all rabbit caecum samples was observed. dgge profiling also showed that the methanogen community from the new zealand white adult rabbits is different and less complex than the methanogen communities from the rumen and pig feces. small subunit ribosomal gene sequences of methanogenic archaea were subsequently re ... | 2010 | 20950344 |
| methanobrevibacter phylotypes are the dominant methanogens in sheep from venezuela. | rumen methanogens in sheep from venezuela were examined using 16s rrna gene libraries and denaturing gradient gel electrophoresis (dgge) profiles prepared from pooled and individual pcr products from the rumen contents from 10 animals. a total of 104 clones were examined, revealing 14 different 16s rrna gene sequences or phylotypes. of the 14 phylotypes, 13 (99 of 104 clones) belonged to the genus methanobrevibacter, indicating that the genus methanobrevibacter is the most dominant component of ... | 2008 | 18165875 |
| description of methanobrevibacter gottschalkii sp. nov., methanobrevibacter thaueri sp. nov., methanobrevibacter woesei sp. nov. and methanobrevibacter wolinii sp. nov.. | formal nomenclature is proposed for five methanogens, isolated from horse, pig, cow, goose and sheep faeces, that represent four novel species of the genus methanobrevibacter. the four species, methanobrevibacter gottschalkii sp. nov., methanobrevibacter thaueri sp. nov., methanobrevibacter woesei sp. nov. and methanobrevibacter wolinii sp. nov., are distinguished from each other by a lack of genomic dna reassociation and from previously described members of the genus on the basis of differences ... | 2002 | 12054244 |