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incrimination of lutzomyia cruzi as a vector of american visceral leishmaniasis. 19989737605
isolation and identification of 9-methylgermacrene-b as the putative sex pheromone of lutzomyia cruzi (mangabeira, 1938) (diptera: psychodidae).lutzomyia (lutzomyia) cruzi has been named as a probable vector of leishmania chagasi in corumbá, mato grosso do sul, brazil. taxonomically l. cruzi is closely related to the l. longipalpis species complex. females of l. cruzi and l. longipalpis are morphologically indistinguishable and associated males must be examined carefully to confirm identifications. chemical analysis hexane extracts of male l. cruzi has revealed the presence of a 9-methylgermacrene-b (c16), a homosesquiterpene (mw 218) p ...200212048580
canine leishmaniosis in south america.abstract : canine leishmaniosis is widespread in south america, where a number of leishmania species have been isolated or molecularly characterised from dogs. most cases of canine leishmaniosis are caused by leishmania infantum (syn. leishmania chagasi) and leishmania braziliensis. the only well-established vector of leishmania parasites to dogs in south america is lutzomyia longipalpis, the main vector of l. infantum, but many other phlebotomine sandfly species might be involved. for quite som ...200919426440
[evidence of transmission of visceral leishmaniasis by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil].the municipality of jaciara was classified in 2003 as a transmission area for visceral leishmaniasis in outbreak situations. this study aimed to establish evidence of transmission of leishmania (leishmania) infantum chagasi by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil.201121340413
proceedings of the ix international symposium on phlebotomine sandflies (isops ix), reims, france, june 28th-july 1st, 2016. 201627677701
dna barcoding of neotropical sand flies (diptera, psychodidae, phlebotominae): species identification and discovery within brazil.dna barcoding has been an effective tool for species identification in several animal groups. here, we used dna barcoding to discriminate between 47 morphologically distinct species of brazilian sand flies. dna barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23-19.04%), indicating cryptic diversity. ...201526506007
leishmania amazonensis dna in wild females of lutzomyia cruzi (diptera: psychodidae) in the state of mato grosso do sul, brazil.studies on natural infection by leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. this study sought to investigate the natural infection by leishmaniain wild female sandflies. the specimens were caught in the city of corumbá, state of mato grosso do sul (brazil) between october 2012-march 2014, and dissected to investigate flagellates and/or submitted to molecular analy ...201526602870
molecular detection of leishmania in phlebotomine sand flies (diptera: psychodidae) from a cutaneous leishmaniasis focus atxakriabá indigenous reserve, brazil.autochthonous cases of american cutaneous leishmaniasis (acl) have been reported since 2001 in the xakriabá indigenous reserve located in the municipality of são joão das missões in northern minas gerais state, brazil. in order to study the presence of leishmania dna in phlebotomine sand flies, six entomological collections were carried out from july 2008 through july 2009, using 40 light traps placed in peridomicile areas of 20 randomly selected houses. from october 2011 through august 2012, an ...201525853254
abundance of lutzomyia longipalpis in urban households as risk factor of transmission of visceral leishmaniasis.urban occurrence of human and canine visceral leishmaniasis (vl) is linked to households with characteristics conducive to the presence of sand flies. this study proposes an ad hoc classification of households according to the environmental characteristics of receptivity to phlebotominae and an entomological study to validate the proposal. here we describe the phlebotominae population found in intra- and peridomiciliary environments and analyse the spatiotemporal distribution of the vl vector lu ...027223866
acoustic signals in the sand fly lutzomyia (nyssomyia) intermedia (diptera: psychodidae).acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. here we report the courtship songs of the sand fly lutzomyia (nyssomyia) intermedia, one of the main vectors of cutaneous leishmaniasis in brazil.201121569534
bacterial diversity of the american sand fly lutzomyia intermedia using high-throughput metagenomic sequencing.parasites of the genus leishmania cause a broad spectrum of diseases, collectively known as leishmaniasis, in humans worldwide. american cutaneous leishmaniasis is a neglected disease transmitted by sand fly vectors including lutzomyia intermedia, a proven vector. the female sand fly can acquire or deliver leishmania spp. parasites while feeding on a blood meal, which is required for nutrition, egg development and survival. the microbiota composition and abundance varies by food source, life sta ...201627581188
acoustic communication in insect disease vectors.acoustic signalling has been extensively studied in insect species, which has led to a better understanding of sexual communication, sexual selection and modes of speciation. the significance of acoustic signals for a blood-sucking insect was first reported in the xix century by christopher johnston, studying the hearing organs of mosquitoes, but has received relatively little attention in other disease vectors until recently. acoustic signals are often associated with mating behaviour and sexua ...024473800
geographic distribution of phlebotomine sandfly species (diptera: psychodidae) in central-west brazil.this study updates the geographic distributions of phlebotomine species in central-west brazil and analyses the climatic factors associated with their occurrence. the data were obtained from the entomology services of the state departments of health in central-west brazil, scientific collections and a literature review of articles from 1962-2014. ecological niche models were produced for sandfly species with more than 20 occurrences using the maxent algorithm and eight climate variables. in all, ...201526018450
multi-modal analysis of courtship behaviour in the old world leishmaniasis vector phlebotomus argentipes.the sand fly phlebotomus argentipes is arguably the most important vector of leishmaniasis worldwide. as there is no vaccine against the parasites that cause leishmaniasis, disease prevention focuses on control of the insect vector. understanding reproductive behaviour will be essential to controlling populations of p. argentipes, and developing new strategies for reducing leishmaniasis transmission. through statistical analysis of male-female interactions, this study provides a detailed descrip ...201425474027
investigation of the bacterial communities associated with females of lutzomyia sand fly species from south america.phlebotomine sand flies are vectors of leishmania that are acquired by the female sand fly during blood feeding on an infected mammal. leishmania parasites develop exclusively in the gut lumen during their residence in the insect before transmission to a suitable host during the next blood feed. female phlebotomine sand flies are blood feeding insects but their life style of visiting plants as well as animals, and the propensity for larvae to feed on detritus including animal faeces means that t ...201222880020
sybr green-based real-time pcr targeting kinetoplast dna can be used to discriminate between the main etiologic agents of brazilian cutaneous and visceral leishmaniases.abstract: background: leishmaniases control has been hampered by the unavailability of rapid detection methods and the lack of suitable therapeutic and prophylactic measures. accurate diagnosis, which can distinguish between leishmania isolates, is essential for conducting appropriate prognosis, therapy and epidemiology. molecular methods are currently being employed to detect leishmania infection and categorize the parasites up to genus, complex or species level. real-time pcr offers several a ...201222240199
phlebotomine sand flies and canine infection in areas of human visceral leishmaniasis, cuiabá, mato grosso.visceral leishmaniasis is a systemic infectious disease that can cause to a severe, potentially life-threatening chronic condition in humans. risk factors for infection in urban areas have been associated with poor living conditions, the presence of sand fly vectors and infected pets. this study aimed to describe sand fly and canine infection in the neighborhoods of human visceral leishmaniasis occurrence in the city of cuiabá, mato grosso state, central-western brazil, reported between january ...201121961754
lovesongs and period gene polymorphisms indicate lutzomyia cruzi (mangabeira, 1938) as a sibling species of the lutzomyia longipalpis (lutz and neiva, 1912) complex.the sand fly lutzomyia cruzi (mangabeira, 1938) is implicated as a vector of american visceral leishmaniasis (avl) in some areas of brazil. lutzomyia cruzi is closely related to lutzomyia longipalpis s.l. (lutz and neiva, 1912) the main latin american vector of avl and a species complex. although females of the two species are identical, the males can be distinguished by differences in the genitalia. nevertheless, pheromone analysis shows that lu. cruzi males produce 9-methyl-germacrene-b, which ...201020478408
detection of natural infection in lutzomyia cruzi and lutzomyia forattinii (diptera: psychodidae: phlebotominae) by leishmania infantum chagasi in an endemic area of visceral leishmaniasis in brazil using a pcr multiplex assay.in order to identify lutzomyia spp. naturally infected by leishmania parasites a pcr multiplex assay coupled to non-isotopic hybridization was used for the analysis of insect samples collected by cdc light traps in an endemic area of visceral leishmaniasis (vl) in the municipality of corumbá, mato grosso do sul state, brazil in may/june 2006. wild sand flies were identified and grouped into pools of 10 female specimens and 27 groups in total were collected. positive results were obtained from lu ...200818502392
[the spread of the visceral leishmaniasis epidemic in the state of mato grosso, 1998-2005].an epidemic of visceral leishmaniasis began in 1998, in the metropolitan region of cuiabá, the capital of the state of mato grosso, brazil. today, it has reached 34 (24.1%) of the 141 municipalities in the state. between january 1998 and december 2005, 138 autochthonous cases were notified, mainly in males (58%), children aged 0-9 years (51.5%) and inhabitants of urban areas (66.7%). canine visceral leishmaniasis has been detected in 41 municipalities, with positive serum in 9% of the 40,000 dog ...200717486252
[spatial distribution of lutzomyia longipalpis (lutz & neiva, 1912) and lutzomyia cruzi (mangabeira, 1938) in the state of mato grosso].visceral leishmaniasis is currently considered an emergent and reemergent disease, in agricultural and urban zones, both in domestic and peridomestic areas. the objective of this work was to verify the spatial distribution of lutzomyia longipalpis and lutzomyia cruzi in mato grosso state. data from 1996 to 2004 was collected by the entomology laboratory, whose captures were carried with a cdc light trap. sixty-eight of the 139 cities in the state have been studied. lutzomyia longipalpis and lutz ...200617119747
the presence of lutzomyia longipalpis in a focus of american visceral leishmaniasis where the only proven vector is lutzomyia cruzi. corumbá, mato grosso do sul state.the present communication reports the presence of lutzomyia longipalpis in corumbá, mato grosso do sul, where the principal vector is lutzomyia cruzi.200314576882
[phlebotomines (diptera: psychodidae) focusing visceral leishmaniasis in the state of mato grosso do sul, brazil].in the americas, lutzomyia longipalpis has been incriminated as the vector of visceral leishmaniasis in almost all the areas in which this disease has been reported. the notification of human cases of visceral leishmaniasis and the presence of dogs with an appearance suggestive of the disease in the country of corumbá, mato grosso do sul state, brazil, led us to undertake an entomological investigation in this area, for the purpose of identifying the phlebotomine vector.19979595767
participation of ticks in the infectious cycle of canine visceral leishmaniasis, in teresina, piauí, brazil.in this study, we detected leishmania spp. infection in r. sanguineus collected from dogs that were naturally infected with l. (l.) infantum. we examined 35 dogs of both sexes and unknown ages. the infected dogs were serologically positive by the immunofluorescence antibody test (ifat), enzyme-linked immunosorbent assay (elisa), and quick test-dpp (dual path platform), as well as parasitological examination of a positive skin biopsy or sternal bone marrow aspiration. ten negative dogs were inclu ...025076429
a comparison of molecular markers to detect lutzomyia longipalpis naturally infected with leishmania (leishmania) infantum.the aim of the present study was to detect natural infection by leishmania (leishmania) infantum in lutzomyia longipalpis captured in barcarena, state of pará, brazil, through the use of three primer sets. with this approach, it is unnecessary to previously dissect the sandfly specimens. dna of 280 lu. longipalpis female specimens were extracted from the whole insects. pcr primers for kinetoplast minicircle dna (kdna), the mini-exon gene and the small subunit ribosomal rna (ssu-rrna) gene of lei ...201425004147
spatiotemporal analysis of sandfly fauna (diptera: psychodidae) in an endemic area of visceral leishmaniasis at pantanal, central south america.environmental changes caused by urbanization can cause alterations in the ecology and behavior of sandflies and in the epidemiology of leishmaniasis. geotechnological tools allow the analysis and recognition of spatiotemporal patterns by monitoring and mapping risk areas of this vector-borne disease. this study aims to describe the sandfly fauna in the municipality of corumbá and to compare it with the data described in a three-year period from 1984 to 1986 by galati. a further aim was to analyz ...201425128480
geographical expansion of canine visceral leishmaniasis in rio de janeiro state, brazil.visceral leishmaniasis (vl) is a vector-borne disease that affects humans, and domestic and wild animals. it is caused by the protozoan leishmania (leishmania) infantum (syn = leishmania chagasi). the domestic dog (canis familiaris) is considered the main reservoir of the etiologic agent of vl in domestic and peridomestic environments. in the past three years, although control actions involving domestic dogs are routinely performed in endemic areas of the rio de janeiro state, new cases of canin ...026603233
sensitivity and specificity of parallel or serial serological testing for detection of canine leishmania infection.in brazil, human and canine visceral leishmaniasis (cvl) caused by leishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. until recently, the brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (elisa) and indirect immunofluorescence assays (ifa) as the screening and confirmatory methods, respectively, for the detection of canine infection. the purpose ...201626910354
performance of conventional pcrs based on primers directed to nuclear and mitochondrial genes for the detection and identification of leishmania spp.in visceral leishmaniasis, the detection of the agent is of paramount importance to identify reservoirs of infection. here, we evaluated the diagnostic attributes of pcrs based on primers directed to cytochrome-b (cytb), cytochrome-oxidase-subunit ii (coxii), cytochrome-c (cytc), and the minicircle-kdna. although pcrs directed to cytb, coxii, cytc were able to detect different species of leishmania, and the nucleotide sequence of their amplicons allowed the unequivocal differentiation of species ...201627253743
monthly distribution of phlebotomine sand flies, and biotic and abiotic factors related to their abundance, in an urban area to which visceral leishmaniasis is endemic in corumbá, brazil.the monthly distribution and abundance of sand flies are influenced by both biotic and abiotic factors. the present study aimed to evaluate the seasonal distribution of sand flies and the relation between their abundance and environmental parameters, including vegetation and climate. this study was conducted over a 2-year period (april 2012 to march 2014). monthly distribution was evaluated through the weekly deployment of cdc light traps in the peridomicile area of 5 residences in an urban area ...201627783667
the lutzomyia longipalpis complex: a brief natural history of aggregation-sex pheromone communication.in this paper we review the natural history of pheromone communication and the current diversity of aggregation-sex pheromones in the sand fly lutzomyia longipalpis. this species complex is the main vector of leishmania infantum, the agent of visceral leishmaniasis in the americas. the identification of variation in pheromone chemotypes combined with molecular and sound analyses have all contributed to our understanding of the extent of divergence among cryptic members of this complex. the impor ...201627842601
lutzomyia longipalpis urbanisation and control.since the description of lutzomyia longipalpis by lutz and neiva more than 100 years ago, much has been written in the scientific literature about this phlebotomine species. soares and turco (2003) and lainson and rangel (2005) have written extensive reviews focused on vector-host-parasite interactions and american visceral leishmaniasis ecology. however, during the last two decades, the success of lu. longipalpis in colonising urban environments and its simultaneous geographical spreading have ...201526517497
predicting the geographic distribution of lutzomyia longipalpis (diptera: psychodidae) and visceral leishmaniasis in the state of mato grosso do sul, brazil.to understand the geographic distribution of visceral leishmaniasis (vl) in the state of mato grosso do sul (ms), brazil, both the climatic niches of lutzomyia longipalpis and vl cases were analysed. distributional data were obtained from 55 of the 79 counties of ms between 2003-2012. ecological niche models (enm) of lu. longipalpis and vl cases were produced using the maximum entropy algorithm based on eight climatic variables. lu. longipalpis showed a wide distribution in ms. the highest clima ...024402151
morphological changes in the bone marrow of the dogs with visceral leishmaniasis.the aim of this study was to evaluate the most frequent lesions in the bone marrow of dogs naturally infected by leishmania (leishmania) chagasi. thirty-three dogs sacrificed at the zoonosis control center of araçatuba, a municipality endemic for visceral leishmaniasis (vl), were used. the animals were classified as asymptomatic, oligosymptomatic, and symptomatic groups. at the necropsy, bone marrow samples were collected from the femur, fixed, processed, and stained with hematoxylin and eosin. ...201424744957
catalogue of the type material of phlebotominae (diptera, psychodidae) deposited in the instituto evandro chagas, brazil.the available type material of phlebotominae (diptera, psychodidae) deposited in the "coleção de flebotomíneos" of the instituto evandro chagas (colfleb iec) is now presented in an annotated catalogue comprising a total of 121 type specimens belonging to 12 species as follow: nyssomyia richardwardi (2 female paratypes), nyssomyia shawi (9 male and 25 female paratypes), nyssomyia umbratilis (female holotype and 1 female paratype), nyssomyia yuilli yuilli (1 male and 1 female paratypes), pintomyia ...201424715786
phlebotomine fauna, natural infection rate and feeding habits of lutzomyia cruzi in jaciara, state of mato grosso, brazil.visceral leishmaniasis (vl) in brazil is transmitted by the phlebotomine lutzomyia longipalpis and in some midwestern regions by lutzomyia cruzi. studies of the phlebotomine fauna, feeding habits and natural infection rate by leishmania contribute to increased understanding of the epidemiological chain of leishmaniases and their vectorial capacity. collections were performed in jaciara, state of mato grosso from 2010-2013, during which time 2,011 phlebotomines (23 species) were captured (68.70% ...201425410993
when population and evolutionary genetics met behaviour.in this review, we analyse the impact of a population and evolutionary genetics approach on the study of insect behaviour. our attention is focused on the model organism drosophila melanogaster and several other insect species. in particular, we explore the relationship between rhythmic behaviours and the molecular evolution of clock and ion channel genes.024473805
functional compounds from the integument of adult red palm weevil rhynchophorus ferrugineus.this study deals with the analysis of the integument of the red palm weevil rhynchophorus ferrugineus of both sexes using the gc-ms technique. the results of the study revealed many promising compounds. these include aspidofractinine-3-methanol (kopsinyl alcohol) which was found in the acetone extract of the sternum of females, and 3-buten-2-ol (32-b) which was found in the extracts of sternum and tergum of males. this compound the aggregation pheromone was secreted by males. additionally, compo ...201324955013
expression of the mevalonate pathway enzymes in the lutzomyia longipalpis (diptera: psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach.in latin america, lutzomyia longipalpis is the main vector of the protozoan parasite leishmania infantum, which is the causal agent of american visceral leishmaniasis. this insect uses male-produced pheromones for mate recognition. elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. motivated by our recent results of the transcriptomic characterization of the l. longipalpis pheromone gl ...201324185139
sensitive and specific serodiagnosis of leishmania infantum infection in dogs by using peptides selected from hypothetical proteins identified by an immunoproteomic approach.in brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (cvl) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. in addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of cvl have been reported. the present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the ...201323554466
fixed differences in the paralytic gene define two lineages within the lutzomyia longipalpis complex producing different types of courtship songs.the sand fly lutzomyia longipalpis (diptera: psychodidae: phlebotominae), the most important vector of american visceral leishmaniasis, is widely distributed in latin america. there is currently a consensus that it represents a species complex, however, the number and distribution of the different siblings is still uncertain. previous analyses have indicated that brazilian populations of this vector can be divided into two main groups according to the type of courtship song (burst vs. pulse) mal ...201222970200
the genetic structure of leishmania infantum populations in brazil and its possible association with the transmission cycle of visceral leishmaniasis.leishmania infantum is the etiologic agent of visceral leishmaniasis (vl) in the americas, mediterranean basin and west and central asia. although the geographic structure of l. infantum populations from the old world have been described, few studies have addressed the population structure of this parasite in the neotropical region. we employed 14 microsatellites to analyze the population structure of the l. infantum strains isolated from humans and dogs from most of the brazilian states endemic ...201222606248
vector competence of lutzomyia cruzi naturally demonstrated for leishmania infantum and suspected for leishmania amazonensis.corumbá city is one of the oldest visceral leishmaniasis-endemic foci in the state of mato grosso do sul, brazil, where the transmission of leishmania infantum has been attributed to lutzomyia cruzi aiming at investigating the parameters of the vectorial capacity of lu. cruzi for l. infantum, a project was undertaken in this city. among these parameters, vector competence was investigated and the results obtained are reported herein. of the 12 hamsters exposed to feed wild-caught female sandflie ...201728077746
experimental infection and transmission of leishmania by lutzomyia cruzi (diptera: psychodidae): aspects of the ecology of parasite-vector interactions.several parameters should be addressed before incriminating a vector for leishmania transmission. those may include its ability to become infected by the same leishmania species found in humans, the degree of attractiveness for reservoirs and humans and capacity to sustain parasite infection under laboratory conditions. this study evaluated the vectorial capacity of lutzomyia cruzi for leishmania infantum and gathered information on its ability to harbor l. amazonensis. laboratory-reared lu. cru ...201728234913
rapid identification of mitochondrial cytochrome b haplotypes by single strand conformation polymorphism in lutzomyia longipalpis (diptera: psychodidae) populations.newly designed primers amplified a 365-bp fragment including the 3' end of mitochondrial cytochromeb (cyt b). field collected specimens of the sand fly, lutzomyia longipalpis (lutz and neiva), from two brazil locations provided test dna. the primers, based on consensus sequences from a taxonomic spectrum of arthropods, have also amplified dna successfully from lutzomyia shannoni dyar, lutzomyia cruzi (mangabeira), and the mosquito aedes albopictus (skuse). single strand conformation polymorphism ...200212144306
sandfly fauna (diptera: psychodidae) in an urban area, central-west of brazil.biological and ecological relations among vectors and their pathogens are important to understand the epidemiology of vector-borne diseases. camapuã is an endemic area for visceral and tegumentary leishmaniasis. the aim of this study was to characterize the sandfly fauna present in camapuã , ms, brazil. sand flies were collected every fortnight from may 2014 to april 2015 using automatic light traps in the domicile and peridomicile of twelve neighborhoods and forest. the collected specimens were ...201728902295
morphometric analysis of longipalpis (diptera: psychodidae) complex populations in mato grosso do sul, brazil.owing to the existence of cryptic species that are difficult to distinguish morphologically, the search for new taxonomic characters and methods for identifying and classifying sand flies continues. lutzomyia longipalpis (lutz & neiva, 1912) and lutzomyia cruzi (mangabeira, 1938) (diptera: psychodidae) are two such species that occur in sympatry in some regions of mato grosso do sul state (ms). twenty females and twenty males from each of the five populations of lu. longipalpis and one populatio ...201526334809
phlebotomine fauna, natural infection rate and feeding habits of lutzomyia cruzi in jaciara, state of mato grosso, brazil.visceral leishmaniasis (vl) in brazil is transmitted by the phlebotomine lutzomyia longipalpis and in some midwestern regions by lutzomyia cruzi. studies of the phlebotomine fauna, feeding habits and natural infection rate by leishmania contribute to increased understanding of the epidemiological chain of leishmaniases and their vectorial capacity. collections were performed in jaciara, state of mato grosso from 2010-2013, during which time 2,011 phlebotomines (23 species) were captured (68.70% ...201425338156
phlebotomine sandflies fauna (diptera: psychodidae) at rural settlements in the municipality of cáceres, state of mato grosso, brazil.leishmaniasis is expanding across brazil, including the state of mato grosso (mt). the aim of this study was to characterize the phlebotomine sandfly fauna at threerural settlements located in the municipality of cáceres, mt, from august 2010 to july 2011.201222836661
phlebotomine fauna (diptera: psychodidae: phlebotominae) in a residential area and in a fragment of savanna vegetation in the municipality of pontal do araguaia, mato grosso, brazil.identification of phlebotomine species in endemic areas is fundamental for analyzing the eco-epidemiological determinants of leishmaniasis. this study had the aim of investigating the phlebotomine fauna in an urban area and in a fragment of native savanna in the municipality of pontal do araguaia, state of mato grosso, brazil, using cdc light traps. one hundred and twenty-three phlebotomine specimens belonging to seventeen different species were caught. our results indicate synanthropic potentia ...201121722493
ultrastructural cytochemistry of the sex pheromone glands of lutzomyia cruzi male sand flies (diptera: psychodidae: phlebotominae).the sex pheromone glands of lutzomyia cruzi male sand flies (diptera: psychodidae) were analyzed by cytochemical techniques. in adult males, the epithelium at the fourth abdominal tergite is modified into a glandular epithelium, with large columnar gland cells located side by side. the gland cell cytoplasm contains a large number of mitochondria and peroxisomes, the latter with positive (electron-dense) reaction for catalase, a typical peroxisomal enzyme marker. the gland cell cytoplasm also con ...200418089046
patterns of exochorion ornaments on eggs of seven south american species of lutzomyia sand flies (diptera: psychodidae).the patterns of exochorion ornaments on eggs of seven south american lutzomyia sand fly species were analyzed by scanning electron microscopy (sem): lutzomyia (lutzomyia) cruzi (mangabeira 1938), lutzomyia (micropygomyia) evandroi (costa lima and antunes 1936), l. (nyssomyia) intermedia (lutz and neiva 1912), l. longipalpis (lutz and neiva 1912), l. migonei (franca 1920), l. (nyssomyia) neivai (pinto 1926), and l. renei (martins, falcao, and silva 1957). different patterns were observed, which s ...200415535608
alternative method for the mass rearing of lutzomyia (lutzomyia) cruzi (diptera: psychodidae) in a laboratory setting.the understanding of the transmission dynamics of leishmania spp. ross as well as the epidemiology and spread of leishmaniasis is related to parasite-vector-host interactions. these interactions can be studied using specimens of a sand fly population reared in the laboratory, exposing individuals to experimental infection for the investigation of vector competence and parameters of the vectorial capacity of the species. the present study sought to describe an alternative method for the implantat ...201526336242
occurrence and probability maps of lutzomyia longipalpis and lutzomyia cruzi (diptera: psychodidae: phlebotominae) in brazil.leishmaniases are serious diseases caused by trypanosomatid protozoans of the genus leishmania transmitted by the bite of phlebotomine sand flies. we analyzed records pertaining to lutzomyia longipalpis (lutz and neiva, 1912) and lutzomyia cruzi (mangabeira, 1938) in brazil from the following sources: the collection of phlebotomine sand flies of the centro de pesquisas rené rachou/fiocruz (fiocruz-colfleb), the "specieslink" (cria) database, from systematic surveys of scientific articles and gra ...201728472338
ultrastructure of male sex pheromone glands in abdominal tergites of five lutzomyia sandfly species (diptera: psychodidae).the fine structure of male sex pheromone disseminating structures on abdominal segments of five species of lutzomyia sandflies (diptera: psychodidae) was analyzed. scanning electron microscopy showed that in lutzomyia cruzi [mem. inst. oswaldo cruz 33 (1938) 349] and lutzomyia longipalpis [mem. inst. oswaldo cruz 4 (1912) 84], the disseminating structures are located in pale spots on abdominal tergites iv or iii and iv and are morphologically similar, appearing as small round cuticular elevation ...200218088957
the role of wing geometric morphometrics in the identification of sandflies within the subgenus lutzomyia.the lutzomyia subgenus (diptera: psychodidae) includes sibling species with morphologically indistinguishable females. the aims of this study were to analyse variations in the size and shape of wings of species within the lutzomyia subgenus and to assess whether these analyses might be useful in their identification. wings (nā€‰=ā€‰733) of 18 species deposited in brazilian collections were analysed by geometric morphometrics, using other genera and subgenera as outgroups. shape variation was summari ...201728707301
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