the dnaj gene as a novel phylogenetic marker for identification of vibrio species.the utility of the dnaj gene for identifying vibrio species was investigated by analyzing dnaj sequences of 57 type strains and 22 clinical strains and comparing sequence homologies with those of the 16s rdna gene and other housekeeping genes (reca, rpoa, hsp60). among the 57 vibrio species, the mean sequence similarity of the dnaj gene (77.9%) was significantly less than that of the 16s rdna gene (97.2%), indicating a high discriminatory power of the dnaj gene. most vibrio species were, therefo ...200717207598
vibrio rotiferianus sp. nov., isolated from cultures of the rotifer brachionus plicatilis.five gram-negative bacterial strains, oxidase-positive, motile by means of more than one polar flagella, facultative anaerobe, arginine dihydrolase-negative, lysine- and omithine decarboxylase-positive, sensitive to the vibriostatic agent o/129, were isolated from a flow-through rotifer culture system in gent, belgium, and previously characterized by fluorescent amplified fragment length polymorphism. comparison of the 16s rdna sequence of strain lmg 21460t indicated close relationships (approxi ...200312656179
integration of a laterally acquired gene into a cell network important for growth in a strain of vibrio rotiferianus.abstract:201122093957
molecular identification of vibrio harveyi-related isolates associated with diseased aquatic organisms.fifty strains belonging to vibrio harveyi, vibrio campbellii, and the recently described vibrio rotiferianus, were analysed using phenotypic and genomic techniques with the aim of analysing the usefulness of the different techniques for the identification of v. harveyi-related species. the species v. harveyi and v. campbellii were phenotypically indistinguishable by more than 100 phenotypic features. thirty-nine experimental strains were phenotypically identified as v. harveyi, but faflp, rep-pc ...200415184563
the vibrio core group induces yellow band disease in caribbean and indo-pacific reef-building determine the relationship between yellow band disease (ybd)-associated pathogenic bacteria found in both caribbean and indo-pacific reefs, and the virulence of these pathogens. ybd is one of the most significant coral diseases of the tropics.200818798767
impact of environmental bacterial communities on fish health in marine recirculating aquaculture systems.marine cultured fish diseases caused by bacteria in recirculating aquaculture systems (rass) greatly threaten fish aquaculture. to date, the dynamics of bacterial populations in ras and their impacts to fish health remain largely unknown. in the present study, the bacterial communities in the water from two different marine rass were analyzed using pyrosequencing technique. fish disease syndromes and mortality had been reported from one ras (ras-d) while the fish in the other ras remained health ...201728619164
interspecific transmission and recovery of tcbs-induced disease between acanthaster planci and linckia guildingi.the susceptibility of the coral-feeding crown-of-thorns starfish acanthaster planci to disease may provide an avenue with which to effectively control population outbreaks that have caused severe and widespread coral loss in the indo-pacific. injecting thiosulfate-citrate-bile-sucrose (tcbs) agar into a. planci tissues induced a disease characterized by dermal lesions, loss of skin turgor, collapsed spines, and accumulation of mucus on spine tips. moreover, the symptoms (and presumably the agent ...201222968793
detection of acylated homoserine lactones produced by vibrio spp. and related species isolated from water and aquatic organisms.aims:  to assess the diversity in production of acylated homoserine lactones (ahls) among vibrio spp and related species. methods and results:  a total of 106 isolates, with representatives of 28 vibrio spp and related species, were investigated for the production of ahls. for this, a rapid method for the screening of ahls was developed based on the use of bacterial biosensors using a double-layer microplate assay. at least one bacterial biosensor was activated in 20 species, agrobacterium tumef ...201122098208
the function of integron-associated gene cassettes in vibrio species: the tip of the iceberg.the integron is a genetic element that incorporates mobile genes termed gene cassettes into a reserved genetic site via site-specific recombination. it is best known for its role in antibiotic resistance with one type of integron, the class 1 integron, a major player in the dissemination of antibiotic resistance genes across gram negative pathogens and commensals. however, integrons are ancient structures with over 100 classes (including class 1) present in bacteria from the broader environment. ...201324367362
deletion of integron-associated gene cassettes impact on the surface properties of vibrio rotiferianus dat722.the integron is a genetic recombination system that catalyses the acquisition of genes on mobilisable elements called gene cassettes. in vibrio species, multiple acquired gene cassettes form a cassette array that can comprise 1-3% of the bacterial genome. since 75% of these gene cassettes contain genes encoding proteins of uncharacterised function, how the integron has driven adaptation and evolution in vibrio species remains largely unknown. a feature of cassette arrays is the presence of large ...201323484028
vibrios of the spotted rose snapper lutjanus guttatus steindachner, 1869 from northwestern characterize and identify vibrios present in wild and cultured juvenile snappers (lutjanus guttatus) in northwestern mexico.200717578416
vibrio crosai sp. nov., isolated from a cultured oyster crassostrea gigas.a motile, facultative anaerobic, marine bacterial isolate (caim 1437(t)) was obtained from a cultured oyster (crassostrea gigas) in sonora, méxico. the strain was studied by a phylogenetic analysis based on sequences of the 16s rrna and five housekeeping genes, i.e. ftsz, gapa, pyrh, reca, and topa. comparison of the almost-complete 16s rrna gene sequence with those of other type strains of the genus vibrio showed a close relationship with the type strains of vibrio orientalis and vibrio rotifer ...201424973134
epidemic mortality of the sponge ircinia variabilis (schmidt, 1862) associated to proliferation of a vibrio recent years, several episodes of mass mortality of sessile epibenthic invertebrates, including sponges, have been recorded worldwide. in the present study, we report a disease event on ircinia variabilis recorded in september 2009 along the southern adriatic and ionian seas (apulian coast), with the aim to quantify the mortality incidence on the sponge population, to investigate the effect of the disease on the sponge tissues and to assess whether the disease is associated with vibrios proli ...201222573240
the first genome sequence of vibrio rotiferianus: strain dat722.vibrio rotiferianus is a marine pathogen capable of causing disease in various aquatic organisms. we announce the genome sequence of v. rotiferianus dat722, which has a large chromosomal integron containing 116 gene cassettes and is a model organism for studying the role of this system in vibrio evolution.201121551292
Vibrio jasicida sp. nov., a member of the Harveyi clade, from marine animals (packhorse lobster, abalone, and Atlantic salmon).Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar) between 1994 and 2002. The bacteria are Gram-negative, rod shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow between 0.5-8.0% NaCl (optimum 3.0-6.0%) and at temperatures of 10-37 °C (optimum 25-30 °C). On the ba ...201121984666
unique and conserved genome regions in vibrio harveyi and related species in comparison with the shrimp pathogen vibrio harveyi caim 1792.vibrio harveyi caim 1792 is a marine bacterial strain that causes mortality in farmed shrimp in north-west mexico, and the identification of virulence genes in this strain is important for understanding its pathogenicity. the aim of this work was to compare the v. harveyi caim 1792 genome with related genome sequences to determine their phylogenic relationship and explore unique regions in silico that differentiate this strain from other v. harveyi strains. twenty-one newly sequenced genomes wer ...201526198743
taxonomic revision of harveyi clade bacteria (family vibrionaceae) based on analysis of whole genome sequences.use of inadequate methods for classification of bacteria in the so-called harveyi clade (family vibrionaceae, gammaproteobacteria) has led to incorrect assignment of strains and proliferation of synonymous species. in order to resolve taxonomic ambiguities within the harveyi clade and to test usefulness of whole genome sequence data for classification of vibrionaceae, draft genome sequences of 12 strains were determined and analysed. the sequencing included type strains of seven species: vibrio ...201323710045
complete genome sequence of a giant vibrio bacteriophage vh7d.a vibrio sp. lytic phage vh7d was isolated from seawater of an abalone farm in xiamen, china. the phage was capable of lysing vibrio rotiferianus dsm 17186(t) and vibrio harveyi dsm 19623(t). the complete genome of this phage consists of 246,964 nucleotides with a gc content of 41.31%, which characterized it as a giant vibriophage. here we report the complete genome sequence and major findings from the genomic annotation.201526476690
vibrio inhibens sp. nov., a novel bacterium with inhibitory activity against vibrio species.strain bflp-10(t), isolated from faeces of wild long-snouted seahorses (hippocampus guttulatus), is a gram-negative, motile and facultatively anaerobic rod. this bacterium produces inhibitory activity against vibrio species. phylogenetic analysis based on 16s rrna gene sequences showed that strain bflp-10(t) was a member of the genus vibrio and was most closely related to vibrio owensii (99%), vibrio communis (98.9%), vibrio sagamiensis (98.9%) and vibrio rotiferianus (98.4%). however, multilocu ...201222472571
vibrio owensii sp. nov., isolated from cultured crustaceans in australia.two bacterial strains (dy05(t) and 47666-1) were isolated in queensland, australia, from diseased cultured crustaceans panulirus ornatus and penaeus monodon, respectively. on the basis of 16s rrna gene sequence identity, the strains were shown to belong to the harveyi clade of the genus vibrio. multilocus sequence analysis using five housekeeping genes (rpoa, pyrh, topa, ftsz and mreb) showed that the strains form a monophyletic group with 94.4% concatenated sequence identity to the closest spec ...201020002183
vibrio communis sp. nov., isolated from the marine animals mussismilia hispida, phyllogorgia dilatata, palythoa caribaeorum, palythoa variabilis and litopenaeus vannamei.eight vibrio isolates originating from the marine corals mussismilia hispida and phyllogorgia dilatata and the zoanthids palythoa caribaeorum and palythoa variabilis in brazil and the pacific white shrimp (litopenaeus vannamei) in ecuador were studied by means of a polyphasic approach. the novel isolates formed a tight monophyletic group in the genus vibrio and were closely related to species of the vibrio harveyi group, to which they showed more than 99 % 16s rrna gene sequence similarity. anal ...201120305064
isolation of lytic bacteriophage against vibrio harveyi.the isolation of lytic bacteriophage of vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster panulirus ornatus.201019886890
genomic diversity of vibrios associated with the brazilian coral mussismilia hispida and its sympatric zoanthids (palythoa caribaeorum, palythoa variabilis and zoanthus solanderi).a taxonomic survey of the vibrios associated with the brazilian endemic coral mussismilia hispida and the sympatric zoanthids (i.e. palythoa caribaeorum, palythoa variabilis and zoanthus solanderi).200919291243
development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals.rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ...201424954661
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