| molecular evolution and phylogeny of satellite rna associated with bamboo mosaic potexvirus. | satellite rna of bamboo mosaic potexvirus (satbamv) is a linear rna molecule which encodes a 20-kda nonstructural protein. sequences of seven different satbamv isolates from bamboo hosts in three genera showed 0.7% to 7.5% base variation which spanned the whole rna molecule. however, the putative 20-kda open reading frame was all preserved in these isolates. the phylogenetic relationship based on the nucleotide sequence did not show particular grouping of satbamv from the host in one genus; neit ... | 1997 | 9069181 |
| generation of subgenomic rna directed by a satellite rna associated with bamboo mosaic potexvirus: analyses of potexvirus subgenomic rna promoter. | satellite rna of bamboo mosaic potexvirus (satbamv), a single-stranded positive-sense rna encoding a nonstructural protein of 20 kda (p20), depends on bamboo mosaic potexvirus (bamv) for replication and encapsidation. a full-length cdna clone of satbamv was used to examine the sequences required for the synthesis of potexvirus subgenomic rnas (sgrnas). subgenomic promoter-like sequences (sgps), 107 nucleotides (nt) upstream from the capsid protein (cp) gene of bamv-v, were inserted upstream of t ... | 2000 | 11044078 |
| structural and mutational analyses of cis-acting sequences in the 5'-untranslated region of satellite rna of bamboo mosaic potexvirus. | the satellite rna of bamboo mosaic virus (satbamv) contains on open reading frame for a 20-kda protein that is flanked by a 5'-untranslated region (utr) of 159 nucleotides (nt) and a 3'-utr of 129 nt. a secondary structure was predicted for the 5'-utr of satbamv rna, which folds into a large stem-loop (lsl) and a small stem-loop. enzymatic probing confirmed the existence of lsl (nt 8-138) in the 5'-utr. the essential cis-acting sequences in the 5'-utr required for satbamv rna replication were de ... | 2003 | 12832220 |
| a conserved secondary structure in the hypervariable region at the 5' end of bamboo mosaic virus satellite rna is functionally interchangeable. | satellite rna (satrna) associated with bamboo mosaic virus (bamv) is dependent on bamv for replication and encapsidation. molecular analyses of total rna extracted from bamboo species collected worldwide revealed that 26 out of 61 bamv isolates harbored satbamv. among them, two phylogenetically distinguishable groups, a and b, with a genetic diversity of 6.9 +/- 0.7% were identified. greatest sequence diversity occurred in the 5' untranslated region (utr) that contained one hypervariable region ... | 2004 | 15527838 |
| crucial role of the 5' conserved structure of bamboo mosaic virus satellite rna in downregulation of helper viral rna replication. | satellite rna of bamboo mosaic virus (satbamv), a single-stranded mrna type satellite encoding a protein of 20 kda (p20), depends on the helper bamv for replication and encapsidation. two satbamv isolates, bsf4 and bsl6, exhibit distinctly differential phenotypes in nicotiana benthamiana plants when coinoculated with bamv rna. bsl6 significantly reduces bamv rna replication and suppresses the bamv-induced symptoms, whereas bsf4 does not. by studies with chimeric satbamvs generated by exchanging ... | 2006 | 16474162 |
| movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite rna-mediated expression system. | the intra- and intercellular transport of potexviruses require interactions among viral rna, coat protein and elements of the triple gene block proteins (tgbps). in this study, the requirement of bamboo mosaic virus (bamv) tgbps for movement functions and the compatibilities with those of two potexviruses, potato virus x (pvx) and foxtail mosaic virus (fomv), were examined using a satellite rna-mediated trans-complementation assay system. single or multiple tgbps of bamv, pvx and fomv were expre ... | 2006 | 16603539 |
| the arginine-rich motif of bamboo mosaic virus satellite rna-encoded p20 mediates self-interaction, intracellular targeting, and cell-to-cell movement. | satellite rna of bamboo mosaic virus (satbamv) has a single open reading frame for a nonstructural, rna-binding protein, p20, which facilitates the long-distance movement of satbamv in nicotiana benthamiana. here, we elucidate various biological properties of p20 and the involvement of a single domain in its activities. p20 displayed a strong self-interaction in vitro and in vivo, and cross-linking assays demonstrated its oligomerization. domain mapping, using the bacterial two-hybrid system, in ... | 2006 | 16838788 |
| in vitro replication of bamboo mosaic virus satellite rna. | an in vitro system was applied to analyze the replication of a satellite rna of bamboo mosaic virus (bamv), designated satbamv rna, using solubilized membrane-bound rna-dependent rna polymerase (rdrp) complexes isolated from bamv-infected nicotiana benthamiana. after removal of endogenous templates, the rdrp complexes of bamv catalyzed rna synthesis upon the addition of the full-length positive (+)- or negative (-)-strand satbamv rna transcripts used as templates. both (+)- and (-)-satbamv rna p ... | 2008 | 18538884 |
| subcellular localization and expression of bamboo mosaic virus satellite rna-encoded protein. | the satellite rna of bamboo mosaic virus (satbamv) has a single open reading frame encoding a non-structural protein, p20, which facilitates long-distance movement of satbamv in bamv and satbamv co-infected plants. immunohistochemistry and immunoelectron microscopy revealed that the p20 protein accumulated in the cytoplasm and nuclei in co-infected cells. p20 and the helper virus coat protein (cp) were highly similar in their subcellular localization, except that aggregates of bamv virions were ... | 2009 | 19141462 |
| structural and functional analyses of the 3' untranslated region of bamboo mosaic virus satellite rna. | the 3'-untranslated region (utr) of rna genomes of viruses and satellite rnas plays essential roles in viral replication and transcription. the structural features of the 3'-utr of the satellite rna of bamboo mosaic virus (satbamv) involved in its replication were analyzed in this study. by the use of enzymatic probing, the secondary structure of satbamv 3'-utr was confirmed to comprise two small stem-loops (sla and slb), one large stem-loop (slc), and a poly(a) tail of mainly 75-200 adenylate r ... | 2009 | 19201437 |
| phosphorylation of bamboo mosaic virus satellite rna (satbamv)-encoded protein p20 downregulates the formation of satbamv-p20 ribonucleoprotein complex. | bamboo mosaic virus (bamv) satellite rna (satbamv) depends on bamv for its replication and encapsidation. satbamv-encoded p20 protein is an rna-binding protein that facilitates satbamv systemic movement in co-infected plants. here, we examined phosphorylation of p20 and its regulatory functions. recombinant p20 (rp20) was phosphorylated by host cellular kinase(s) in vitro, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and mutational analyses revealed ser-11 as ... | 2011 | 21965537 |