| isolation and characterization of a new ribosome inactivating protein, momorgrosvin, from seeds of the monk's fruit momordica grosvenorii. | momorgrosvin, a single-chained glycoprotein with a molecular weight of 27.7 kilodaltons and an isoelectric point of about 9 was isolated from the seeds of momordica grosvenorii (family cucurbitaceae). the isolation procedure entailed acetone precipitation, affinity chromatography on hi trap blue, cation exchange chromatography on resource s and size exclusion chromatography on superose 12. the sequence of the first eighteen n-terminal amino acid residues of momorgrosvin exhibited homology to tho ... | 2001 | 11205869 |
| cucurbitane glycosides from the fruits of siraitia gros venorii and their inhibitory effects on epstein-barr virus activation. | six new cucurbitane glycosides, mogroside ii b (2), 11-deoxymogroside iii (4), 7-oxomogroside ii e (5), 7-oxomogroside v (6), 11-oxomogroside ii a1 (7), and 11-oxomogroside iv a (8), and two known but new naturally occurring cucurbitane glycosides, mogroside ii a1 (1) and mogroside iii a2 (3), were isolated from an ethanol extract of the fruits of siraitia grosvenorii. upon evaluation of compounds 1-8 for inhibitory effects against the epstein-barr virus early antigen (ebv-ea) activation induced ... | 2007 | 17477572 |
| effect of siraitia grosvenorii polysaccharide on glucose and lipid of diabetic rabbits induced by feeding high fat/high sucrose chow. | the siraitia grosvenorii polysaccharide (sgp) from the siraitia grosvenorii (swingle) was isolated and purified. the therapeutic effects of sgp on diabetic rabbits induced by feeding high fat/high sucrose chow were studied. after administration of sgp for 4 weeks, the fasting blood glucose (fbg), plasma insulin levels (ins), plasma total cholesterol (tc), triglyceride (tg), and hdl-c were assayed. the results showed that administration of sgp can significantly decrease plasma total cholesterol, ... | 2007 | 18273381 |
| [study on quantitative assessment of siraitia grosvenorii germplasms by general index]. | to establish a weight-marking method and evaluate siraitia grosvenorii germplasms. | 2007 | 18330237 |
| suppressive effect of siraitia grosvenorii extract on dicyclanil-promoted hepatocellular proliferative lesions in male mice. | dicyclanil (dc) generates reactive oxygen species (ros) due to cyp1a1 induction, and dna damage caused by oxidative stress is probably involved in hepatocarcinogenesis in mice. to clarify the modifying effect of the siraitia grosvenorii extract (sge), which has antioxidative properties, we employed a 2-stage liver carcinogenesis model in partially hepatectomized male icr mice. mice maintained on diet containing dc at a concentration of 1,500 ppm for 9 weeks after a single intraperitoneal injecti ... | 2009 | 19182440 |
| antibacterial compounds from siraitia grosvenorii leaves. | luo han guo (lhg) fruits (siraitia grosvenorii swingle) have been used as traditional medicine in china for centuries to treat sore throats and coughs. however, lhg leaves are seldom used and minimal scientific information is available on them. in our recent study on the leaves of s. grosvenorii, the bioactive compounds β-amyrin (2), aloe emodin (5), aloe-emodin acetate (6), 5α,8α-epidioxy-24(r)-methylcholesta-6,22-dien-3β-ol (7) and p-hydroxyl benzyl acid (8), accompanied by n-hexadecaoic acid ... | 2011 | 21547839 |
| [mixed infection caused by meloidogyne and pathogenic fungi on siraitia grosvenorii]. | to study the mixed infection caused by meloidogyne and pathogenic fungi on siraitia grosvenorii,the result can provide basis for controltion. | 2011 | 21818963 |
| molecular and biochemical characterization of squalene synthase from siraitia grosvenorii. | to clone and characterize the squalene synthase from siraitia grosvenorii (sgsqs). | 2017 | 28353148 |
| functional characterization of cucurbitadienol synthase and triterpene glycosyltransferase involved in biosynthesis of mogrosides from siraitia grosvenorii. | mogrosides, the major bioactive components isolated from the fruits of siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (cbq), cyt p450s (p450s) and udp glycosyltransferases (ugts) in vivo. however, the relevant genes have not been ch ... | 2015 | 25759326 |
| oxidation of cucurbitadienol catalyzed by cyp87d18 in the biosynthesis of mogrosides from siraitia grosvenorii. | mogrosides, the principally bioactive compounds extracted from the fruits of siraitia grosvenorii, are a group of glycosylated cucurbitane-type tetracyclic triterpenoid saponins that exhibit a wide range of notable biological activities and are commercially available worldwide as natural sweeteners. the biosynthesis of mogrosides involves initial cyclization of 2,3-oxidosqualene to the triterpenoid skeleton of cucurbitadienol, followed by a series of oxidation reactions catalyzed by cyt p450s (p ... | 2016 | 26903528 |
| biotransformation of mogrosides from siraitia grosvenorii swingle by saccharomyces cerevisiae. | mogrosides are a group of triterpenoidal saponins from the fruit of siraitia grosvenorii swingle; they are intensely sweet and have consequently been used as a substitute for sugar by the food industry. the lack of efficient methods to produce specific mogrosides has hindered investigation of the relationship between their structure and bioactivity, e.g., down-regulation of blood glucose levels, anti-inflammation, and antiviral infection. here, we attempt to selectively convert the major saponin ... | 2013 | 23796186 |
| characterization of conserved micrornas from five different cucurbit species using computational and experimental analysis. | micrornas (mirnas) are ∼21 nt non-coding small rnas which regulate gene expression at the post-transcriptional level in plants and animals. until recently, only limited numbers of mirnas were identified in cucurbitaceae, a large flowering plant family. in this study, 220 potential mirna candidates were identified from five species of cucurbitaceae family using a comparative genome-based computational analysis. a comprehensive bioinformatic analysis of est (expressed sequence tag) and gss (genomi ... | 2014 | 24657600 |
| [extraction of total rna and cloning of sgdhar gene from siraitia grosvenorii]. | total rna was isolated from siraitia grosvenorii fruit by the method of modified trizol, according to s. grosvenorii fruit characteristics of rich phenols, polysaccharide, oil and proteins. the od260/280, od260/230, rna integrity (rin) and yield of the total rna with this method were 2.01, 2.02, 9.50 and 260 mirog.g-1, respectively. the open reading frame (orf) of dehydroascorbate reductase (dhar), named as sgdhar, was cloned by rapid amplification of cdna ends (race) and rt-pcr method from s. g ... | 2014 | 24783516 |
| determination of toxic metals by icp-ms in asiatic and european medicinal plants and dietary supplements. | the potentially toxic metals content was determined in selected plants, used in traditional chinese medicine (angelica sinensis, bacopa monnieri, bupleurum sinensis, curcuma longa, cola accuminata, emblica officinalis, garcinia cambogia, mucuna pruriens, ocimum sanctum, panax ginseng, pueraria lobata, salvia miltiorrhiza, schisandra sinensis, scutellaria baicalensis, siraitia grosvenorii, terminalia arjuna and terminalia chebula), and some european herbs (echinacea purpurea, hypericum perforatum ... | 2015 | 25467854 |