| genomic analysis of clostridium perfringens bacteriophage phi3626, which integrates into guaa and possibly affects sporulation. | two temperate viruses, phi3626 and phi8533, have been isolated from lysogenic clostridium perfringens strains. phage phi3626 was chosen for detailed analysis and was inspected by electron microscopy, protein profiling, and host range determination. for the first time, the nucleotide sequence of a bacteriophage infecting clostridium species was determined. the virus belongs to the siphoviridae family of the tailed phages, the order caudovirales. its genome consists of a linear double-stranded dna ... | 2002 | 12142405 |
| the murein hydrolase of the bacteriophage phi3626 dual lysis system is active against all tested clostridium perfringens strains. | clostridium perfringens commonly occurs in food and feed, can produce an enterotoxin frequently implicated in food-borne disease, and has a substantial negative impact on the poultry industry. as a step towards new approaches for control of this organism, we investigated the cell wall lysis system of c. perfringens bacteriophage phi3626, whose dual lysis gene cassette consists of a holin gene and an endolysin gene. hol3626 has two membrane-spanning domains (msds) and is a group ii holin. a posit ... | 2002 | 12406719 |