the use of collagenase to improve the detection of plant viruses in vector nematodes by rt-pcr. | tomato ringspot virus (torsv), tobacco ringspot virus (trsv) and tobacco rattle virus (trv) are transmitted to healthy plants by viruliferous nematodes in the soil. a method was developed for extraction of genomic viral rna from virus particles carried within nematodes and a sensitive nested rt-pcr detection assay. the procedure has been adapted to microscale for handling multiple samples. this assay is effective for detection of torsv or trsv in xiphinema americanum or trv in paratrichodorus al ... | 2009 | 18992280 |
control of paratrichodorus allius and corky ringspot disease in potato with shank-injected metam sodium. | corky ringspot disease (crs) of potato produces necrotic areas in tubers that are considered quality defects that can lead to crop rejection. crs is caused by tobacco rattle virus that is vectored by stubby-root nematodes (paratrichodorus spp., trichodorus spp.) at very low population densities, making disease management difficult and expensive. fumigation with metam sodium (ms) is a common practice to control soil-borne fungi and increase potato yield. ms is generally applied in water via chemi ... | 2007 | 19259496 |
establishing a corky ringspot disease plot for research purposes. | a method to establish two experimental corky ringspot disease (crs) plots that had no prior crs history is described. crs is a serious disease of potato in the pacific northwest caused by tobacco rattle virus (trv) and transmitted primarily by paratrichodorus allius. 'samsun nn' tobacco seedlings were inoculated with viruliferous p. allius in the greenhouse before they were transplanted into the field soil at the rate of 3,000 plus seedlings/ha. care was taken to keep soil around plants in the g ... | 2007 | 19259504 |
eliminating tobacco rattle virus from viruliferous paratrichodorus allius and establishing a new virus-vector combination. | a reliable method to eliminate tobacco rattle virus (trv) from viruliferous paratrichodorus allius populations was developed. this virus is vectored by p. allius in the pacific northwest and causes corky ringspot disease (crs) of potato. the viruliferous nematodes that were reared on 'vernema' alfalfa or '770' scotch spearmint for at least 3 months did not transmit trv to 'samsun nn' tobacco, a suitable indicator plant, and did not cause crs symptoms on 'russet norkotah' tubers. a new isolate of ... | 2002 | 19265910 |
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994. | previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ... | 1999 | 19270925 |
control of paratrichodorus allius and corky ringspot disease of potato in the columbia basin of oregon. | corky ringspot disease (crs) of potato, caused by tobacco rattle virus that is vectored by stubby-root nematodes (paratrichodorus spp.), is often controlled by aldicarb. when use of aldicarb on potato was suspended in 1989, an increase in crops rejected due to crs in the columbia basin of the u.s. pacific northwest occurred. during 1992-94, several fumigant and nonfumigant nematicides were tested alone and in combination for control of p. allius and crs. aldicarb alone significantly reduced crs ... | 2000 | 19271011 |
the effects of brassica green manures on plant parasitic and free living nematodes used in combination with reduced rates of synthetic nematicides. | brassica plants once incorporated into soil as green manures have recently been shown to have biofumigant properties and have the potential of controlling plant-parasitic nematodes. in washington state, plant-parasitic nematodes are successfully managed with synthetic nematicides. however, some of the synthetic nematicides became unavailable recently or their supply is limited leaving growers with few choices to control plant-parasitic nematodes. the objective of this project was to evaluate the ... | 2011 | 22791922 |
effects of in-furrow and water-run oxamyl on paratrichodorus allius and corky ringspot disease of potato in the klamath basin. | corky ringspot disease (crs) of potato (solanum tuberosum) is caused by the tobacco rattle virus (trv), which is vectored by stubby-root nematodes, paratrichodorus spp. and trichodorus spp., and is a significant threat to potato quality and production in many areas of the western united states. between 2002 and 2005, fields with a history of crs were planted to potato and treated with various combinations of in-furrow (if) and chemigated (water run, wr) oxamyl [methyl n'n'-dimethyl-n-[(methyl ca ... | 2010 | 22736830 |
rapid detection of tobacco rattle tobravirus in viruliferous paratrichodorus allius from greenhouse and field specimens. | the stubby root nematode, paratrichodorus allius, is important to the potato industry in the pacific northwest of usa, because it vectors tobacco rattle virus (trv), the causal agent of corky ringspot disease. the current method for determining if nematodes are viruliferous for trv takes several weeks, requiring a glasshouse bioassay followed by a serological test. to overcome this drawback, a rapid and affordable molecular test was developed using reverse transcription polymerase chain reaction ... | 2009 | 22661778 |
management of paratrichodorus allius damage to onion in the columbia basin of oregon. | production of storage and dehydration (dehy) onions is increasing rapidly in the northcentral oregon region of the columbia basin. roughly circular patches of stunted onions have been observed in many fields and are often numerous and (or) extensive in area. onions in these patches often exhibit symptoms typical of stubby-root nematodes, and paratrichodorus allius has been collected from several damaged areas. this study examined the relationship of p. allius densities to onion yield reduction a ... | 1999 | 19270935 |