| membrane eicosapentaenoic acid is involved in the hydrophobicity of bacterial cells and affects the entry of hydrophilic and hydrophobic compounds. | eicosapentaenoic acid (epa)-producing shewanella marinintestina ik-1 (ik-1) and its epa-deficient mutant ik-1delta8 (ik-1delta8) were grown on microtitre plates at 20 degrees c in a nutrient medium that contained various types of growth inhibitors. the minimal inhibitory concentrations of hydrogen peroxide and tert-butyl hydroxyl peroxide were 100 microm and 1 mm, respectively, for ik-1 and 10 and 100 microm, respectively, for ik-1delta8. ik-1 was much more resistant than ik-1delta8 to the four ... | 2010 | 20370838 |
| the antioxidative function of eicosapentaenoic acid in a marine bacterium, shewanella marinintestina ik-1. | when the eicosapentaenoic acid (epa)-deficient mutant strain ik-1delta8 of the marine epa-producing shewanella marinintestina ik-1 was treated with various concentrations of hydrogen peroxide (h(2)o(2)), its colony-forming ability decreased more than that of the wild type. protein carbonylation, induced by treating cells with 0.01 mm h(2)o(2) under bacteriostatic conditions, was enhanced only in cells lacking epa. the amount of cells recovered from the cultures was decreased more significantly b ... | 2007 | 17706200 |
| shewanella pneumatophori sp. nov., an eicosapentaenoic acid-producing marine bacterium isolated from the intestines of pacific mackerel (pneumatophorus japonicus). | an eicosapentaenoic acid-producing bacterium, previously described as shewanella sp. strain scrc-2738, was classified by phenotypic characterization, chemotaxonomic analysis, 16s rrna gene sequence analysis and dna-dna hybridization. the isolate was gram-negative, rod-shaped and motile by using polar flagella. the strain grew at 4-32 degrees c; the optimum growth temperature was 27 degrees c. nacl was required for growth. the major isoprenoid quinones were ubiquinone-7 and ubiquinone-8 and its d ... | 2005 | 16280496 |
| enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria. | when docosahexaenoic acid (dha)-producing moritella marina strain mp-1 was cultured in the medium containing 0.5 microg cerulenin ml-1, an inhibitor for fatty acid biosynthesis, the cells grew normally, but the content of dha in the total fatty acids increased from 5.9-19.4%. the dha yield of m. marina strain mp-1 cells also increased from 4 to 13.7 mg l-1 by cerulenin treatment. the same effect of cerulenin was observed in eicosapentaenoic acid (epa)-producing shewanella marinintestina strain i ... | 2005 | 15834803 |
| shewanella marinintestina sp. nov., shewanella schlegeliana sp. nov. and shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. | three novel shewanella species are described on the basis of phenotypic, chemotaxonomic and phylogenetic studies. a total of six novel halophilic, aerobic organisms with the ability to produce eicosapentaenoic acid (epa) were isolated from various sea animals in japan. cells of all six isolates were gram-negative, rod-shaped and motile by means of polar flagella. they were able to produce large amounts of epa (about 20% of the total fatty acids) and had isoprenoid quinones q-7 and q-8 as major c ... | 2003 | 12710618 |