Publications

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characterization of the type i secretion system of the rtx toxin apxii in "actinobacillus porcitonsillarum".strains of actinobacillus porcitonsillarum are regularly isolated from the tonsils of healthy pigs. a. porcitonsillarum is non pathogenic but phenotypically it strongly resembles the pathogenic species actinobacillus pleuropneumoniae, thereby interfering with the diagnosis of the latter. a. porcitonsillarum is hemolytic but unlike a. pleuropneumoniae, it contains only apxii genes and not apxi or apxiii genes. in contrast to the truncated apxii operon of a. pleuropneumoniae, which lacks the type ...200515863281
pulmonary isolation of actinobacillus porcitonsillarum in pigs in europe. 200617088304
antimicrobial resistance profile of actinobacillus pleuropneumoniae and actinobacillus porcitonsillarum.a total of 83 actinobacillus pleuropneumoniae and 58 actinobacillus porcitonsillarum strains collected from slaughtered pigs in switzerland were screened for susceptibility to 20 antimicrobial agents by mic determinations. resistance to sulfamethoxazole, the combination sulfamethoxazole-trimethoprim, tiamulin, tilmicosin, tetracycline, penicillin and ampicillin were found. a few a. porcitonsillarum isolates displayed decreased susceptibility to enrofloxacin. pcr analysis revealed the presence of ...200717314014
prevalence of "actinobacillus porcitonsillarum" in porcine tonsils and development of a diagnosis duplex pcr differentiating between "actinobacillus porcitonsillarum" and actinobacillus pleuropneumoniae."actinobacillus porcitonsillarum" is a newly suggested commensal species colonizing porcine tonsils. in the diagnostic laboratory the sole difference to the porcine lung pathogen actinobacillus pleuropneumoniae is a negative mannitol reaction. in order to substantiate and improve this important differentiation a pcr test was developed using the relevant reference strains including actinobacillus minor. the practicability of the test was confirmed on 20 clinical isolates of actinobacillus spp. cu ...200717320315
granulomatous lymphadenitis and pneumonia associated with actinobacillus porcitonsillarum in a slaughter pig.multiple coalescing granulomatous foci were detected in the pulmonary hilar and mediastinal lymph nodes and lung of a slaughtered pig aged 6 months. haemolytic, gram-negative bacilli were isolated from the lymph nodes. the isolate (strain to17214) strongly cross-reacted with sera against actinobacillus pleuropneumoniae serotype 12 in slide agglutination tests. comparative 16s rdna gene sequencing analysis identified strain to17214 as actinobacillus porcitonsillarum. histologically, extensive inf ...200717629969
small multidrug resistance plasmids in actinobacillus porcitonsillarum.the complete nucleotide sequences of six actinobacillus porcitonsillarum plasmids pkma202 (13.425-kb), pkma1467 (11.115-kb), pkma5 (9.549-kb), pimd50 (8.751-kb), pkma505 (8.632-kb) and pkma757 (4.556-kb) and three actinobacillus pleuropneumoniae plasmids ppsas1522 (4.244-kb), pard3079 (3.884-kb) and pkma2425 (3.156-kb) were determined. all the plasmids contain the sulfonamide resistance gene sul2. one a. pleuropneumoniae plasmid and five a. porcitonsillarum plasmids also have the streptomycin re ...200818190962
prevalence of granulomatous pleuropneumonia associated with actinobacillus pleuropneumoniae serotype 2 in slaughter pigs.a total of 14,818 slaughtered pigs were examined macroscopically. of these, 25 pigs with porcine pleuropneumonia were collected and the relations among actinobacillus spp. and granulomatous lesions in organs (lungs and tonsils) were evaluated. in the lungs, only actinobacillus pleuropneumoniae serotype 2 was isolated from 20 of the pigs. histologically, granulomatous pneumonia with a. pleuropneumoniae antigen was detected in 8 of the pigs. the antigen was visible in the centers of the lesions al ...200919721364
production of haemolysins by strains of the actinobacillus minor/"porcitonsillarum" complex.actinobacillus minor and "actinobacillus porcitonsillarum" are distinguished by their haemolytic activities, the latter organism being haemolytic and the former, non-haemolytic. analysis of a whole genome shotgun sequence, however, revealed that a. minor strain 202, like "a. porcitonsillarum", possesses a haemolysin-encoding apxii operon. the purpose of this study was therefore to investigate haemolysin production by this organism and also by three additional members of the a. minor/"porcitonsil ...201019819087
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