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paenibacillus graminis sp. nov. and paenibacillus odorifer sp. nov., isolated from plant roots, soil and food.sixteen gram-positive endospore-forming bacteria previously isolated from soil, plant rhizospheres, plant roots and pasteurized pureed vegetables were studied to determine their taxonomic positions. the isolates were formerly identified as bacillus circulans based on their biochemical characters using api galleries. two of these strains, rsa19t and tod45t, were recently assigned to the genus paenibacillus based on phylogenetic analysis of their 16s rrna (rrs) gene sequence. in the present work, ...200211931174
evaluation of the diversity of cyclodextrin-producing paenibacillus graminis strains isolated from roots and rhizospheres of different plants by molecular methods.to address the diversity of cyclodextrin-producing p. graminis strains isolated from wheat roots and rhizospheres of maize and sorghum sown in australia, brazil, and france, restriction fragment length polymorphism analysis of part of genes encoding rna polymerase (rpob-rflp) and dna gyrase subunit b (gyrb-rflp) was used to produce genetic fingerprints. a phylogenetic tree based on rpob gene sequences was also constructed. the isolates originated from brazil could be separated from those from au ...200617205036
cyclodextrin production and genetic characterization of cyclodextrin glucanotranferase of paenibacillus graminis.paenibacillus graminis strains were described recently as cyclodextrin (cd) producers. cyclodextrins are produced by cyclodextrin glucanotransferase (cgtase) which has not been characterized in p. graminis. similar amounts of alpha- and beta-cds were produced by p. graminis (mc22.13) and p. macerans (lmd24.10(t)). primers were designed to sequence the gene encoding cgtase from p. graminis. a phylogenetic tree was constructed and p. graminis cgtase protein showed to be closer (79.4% protein ident ...200818246304
genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern brazil.in this work, a total of 311 putative nitrogen-fixing bacilli were isolated from seven distinct wheat production zones of the rio grande do sul state, brazil. strains belonging to several species were grouped into 40 different nifh-rflp-pcr profiles. the genus paenibacillus was the most prominent group in both the rhizosphere (77.8%) and soil (79%). paenibacillus borealis was the most frequently identified species, followed by paenibacillus graminis. the remainder of the isolated bacteria belong ...200818490146
paenibacillus sonchi sp. nov., a nitrogen-fixing species isolated from the rhizosphere of sonchus oleraceus.a nitrogen-fixing bacterium, designated strain x19-5(t), was isolated from rhizosphere soil of sonchus oleraceus. phylogenetic analysis based on a fragment of the nifh gene and the full-length 16s rrna gene sequence revealed that strain x19-5(t) was a member of the genus paenibacillus. strain x19-5(t) showed the highest 16s rrna gene sequence similarity (98.8 %) with paenibacillus graminis rsa19(t) and below 97 % similarity with other recognized members of the genus. the level of dna-dna related ...200919625436
paenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of triticum aestivum.a bacterial strain designated sbr5(t) was isolated from the rhizosphere of triticum aestivum. a phylogenetic analysis based on the 16s rrna gene sequence placed the isolate within the genus paenibacillus, being most closely related to paenibacillus graminis rsa19(t) (98.1 % similarity). the isolate was a gram-reaction-variable, motile, facultatively anaerobic bacterium, with spores in a terminal position in cells. starch was utilized and dihydroxyacetone and catalase were produced. strain sbr5(t ...201019648317
paenibacillus jilunlii sp. nov., a nitrogen-fixing species isolated from the rhizosphere of begonia semperflorens.a nitrogen-fixing bacterium, designated strain be17(t), was isolated from rhizosphere soil of begonia semperflorens planted in beijing botanical garden, pr china. phylogenetic analyses based on a segment of the nifh gene sequence and a full-length 16s rrna gene sequence revealed that strain be17(t) was a member of the genus paenibacillus. high levels of 16s rrna gene sequence similarity were found between strain be17(t) and paenibacillus graminis rsa19(t) (97.9 %), paenibacillus sonchi lmg 24727 ...201020601486
identification and characterization of psychrotolerant sporeformers associated with fluid milk production and processing.psychrotolerant spore-forming bacteria represent a major challenge to the goal of extending the shelf life of pasteurized dairy products. the objective of this study was to identify prominent phylogenetic groups of dairy-associated aerobic sporeformers and to characterize representative isolates for phenotypes relevant to growth in milk. analysis of sequence data for a 632-nucleotide fragment of rpob showed that 1,288 dairy-associated isolates (obtained from raw and pasteurized milk and from dai ...201222247129
paenibacillus salinicaeni sp. nov., isolated from saline silt sample.a novel facultatively anaerobic bacterium, designated strain lam0a28(t), was isolated from a saline silt sample collected from the chinese sea of death located in suining city, sichuan province, china. cells of strain lam0a28(t) were observed to be gram-stain positive, motile, endospore-forming and straight-rod shaped. strain lam0a28(t) was found to be able to grow at 15-45 °c (optimum: 30-35 °c), ph 5.0-10.0 (optimum: 7.5) and 0-5 % nacl (w/v) (optimum: 0.5 %). the 16s rrna gene sequence simila ...201626979511
interrelationship of bradyrhizobium sp. and plant growth-promoting bacteria in cowpea: survival and symbiotic performance.the objective of this study was to evaluate the survival of cowpea during bacterial colonization and evaluate the interrelationship of the bradyrhizobium sp. and plant growth-promoting bacteria (pgpb) as a potential method for optimizing symbiotic performance and cowpea development. two experiments using the model legume cowpea cv. "ipa 206" were conducted. in the first experiment, cowpea seeds were disinfected, germinated and transferred to sterilized gibson tubes containing a nitrogen-free nut ...201323456712
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