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a novel c-type lysozyme from mytilus galloprovincialis: insight into innate immunity and molecular evolution of invertebrate c-type lysozymes.a c-type lysozyme (named as mgclyz) gene was cloned from the mussel mytilus galloprovincialis. blast analysis indicated that mgclyz was a salivary c-type lysozyme which was mainly found in insects. the nucleotide sequence of mgclyz was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. the deduced mature peptide of mgclyz was of a calculated molecular weight of 14.4 kd and a theoretical isoelectric point (pi) of 8.08. evolution ...201323818979
biodegradation of the allelopathic chemical m-tyrosine by bacillus aquimaris ssc5 involves the homogentisate central pathway.m-tyrosine is an amino acid analogue, exuded from the roots of fescue grasses, which acts as a potent allelopathic and a broad spectrum herbicidal chemical. although the production and toxic effects of m-tyrosine are known, its microbial degradation has not been documented yet. a soil microcosm study showed efficient degradation of m-tyrosine by the inhabitant microorganisms. a bacterial strain designated ssc5, that was able to utilize m-tyrosine as the sole source of carbon, nitrogen, and energ ...201324098407
bacillus enclensis sp. nov., isolated from sediment sample.a novel bacterial strain, designated sgd-1123(t) was isolated from chorao island, in goa province, india. the strain was found to be able to grow at 15-42 °c, ph 5-12 and 0-12 % (w/v) nacl. the whole cell hydrolysates were found to contain meso-diaminopimelic acid, galactose and arabinose. the major fatty acids were identified as iso-c15:0 and anteiso-c15:0, mk-7 was identified as the predominant menaquinone and the predominant polar lipids were identified as diphosphatidylglycerol, phosphatidyl ...201424174310
metabolomic characterization of halophilic bacterial isolates reveals strains synthesizing rare diaminoacids under salt stress.metabolomics-based approaches to study stress responses in bacteria have received much attention in recent years. in the present study, a metabolomic analysis of the representative halophilic bacterial isolates (halomonas hydrothermalis vitp9, bacillus aquimaris vitp4, planococcus maritimus vitp21 and virgibacillus dokdonensis vitp14) from a saltern region in india was performed using nuclear magnetic resonance spectroscopy. chemometric analysis of (1)h nmr spectra revealed salt-dependent increa ...201424636996
bacillus oryzaecorticis sp. nov., a moderately halophilic bacterium isolated from rice husks.a gram-stain-positive, aerobic, endospore-forming, moderately halophilic rod, designated strain r1(t), was isolated from rice husks and subjected to a taxonomic study using a polyphasic approach. strain r1(t) produced spherical or ellipsoidal endospores at a subterminal position in swollen sporangia, and was catalase- and oxidase-positive. the isolate grew optimally at 37 °c and ph 6.0-7.0, and could grow in the presence of up to 9% (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequen ...201424860111
retraction: biodegradation of the allelopathic chemical m-tyrosine by bacillus aquimaris ssc5 involves the homogentisate central pathway. 201425007283
protein engineering of selected residues from conserved sequence regions of a novel anoxybacillus α-amylase.the α-amylases from anoxybacillus species (aska and adta), bacillus aquimaris (baqa) and geobacillus thermoleovorans (gta, pizzo and gtamyii) were proposed as a novel group of the α-amylase family gh13. an aska yielding a high percentage of maltose upon its reaction on starch was chosen as a model to study the residues responsible for the biochemical properties. four residues from conserved sequence regions (csrs) were thus selected, and the mutants f113v (csr-i), y187f and l189i (csr-ii) and a1 ...201425069018
bacillus haikouensis sp. nov., a facultatively anaerobic halotolerant bacterium isolated from a paddy soil.a gram-stain positive, rod-shaped, endospore-forming and facultatively anaerobic halotolerant bacterium, designated as c-89(t), was isolated from a paddy field soil in haikou, hainan province, people's republic of china. optimal growth was observed at 37 °c and ph 7.0 in the presence of 4% nacl (w/v). the predominant menaquinone was identified as mk-7, the major cellular fatty acids were identified as anteiso-c(15:0) and iso-c(15:0), and the major cellular polar lipids were identified as phospha ...201425100188
biochemical changes induced by salt stress in halotolerant bacterial isolates are media dependent as well as species specific.halophilic bacteria respond to salt stress by regulating the cytosolic pools of organic solutes to achieve osmotic equilibrium. in order to understand the metabolic regulation of these organic solutes, for the first time, we have investigated the effect of salt on growth and biochemical changes in four major moderately halophilic bacterial strains isolated from a saltern region of the kumta coast, india. the strains under study were halomonas hydrothermalis vitp9, bacillus aquimaris vitp4, plano ...201625286020
alkyl hydroperoxide reductase from bacillus aquimaris mksc 6.2 protects esherichia coli from oxidative stress.alkyl hydroperoxide reductase genes (ahpcf) from the soft coral associated bacillus aquimaris mksc6.2 have been isolated. the cloned 546 bp ahpc gene encodes a 181 amino acid residues polypeptide. the ahpc belongs to typical 2-cys peroxiredoxin (prx) containing conserved peroxidatic cysteine residue (c46 ) required for hydroperoxide reduction and conserved resolving cysteine (c166 ). the isolated 1530 bp ahpf gene encodes a polypeptide of 509 amino acid residues with two conserved c128 hnc131 an ...201626523844
crystal structure of anoxybacillus α-amylase provides insights into maltose binding of a new glycosyl hydrolase subclass.a new subfamily of glycosyl hydrolase family gh13 was recently proposed for α-amylases from anoxybacillus species (aska and adta), geobacillus thermoleovorans (gta, pizzo, and gtamyii), bacillus aquimaris (baqa), and 95 other putative protein homologues. to understand this new gh13 subfamily, we report crystal structures of truncated aska (taska). aska is a thermostable enzyme capable of producing high levels of maltose. unlike gta, biochemical analysis showed that ca(2+) ion supplementation enh ...201626975884
solvent tolerant marine bacterium bacillus aquimaris secreting organic solvent stable alkaline cellulase.the organic solvent tolerant bacteria with their physiological abilities to decontaminate the organic pollutants have potentials to secrete extracellular enzymes of commercial importance. of the 19 marine bacterial isolates examined for their solvent tolerance at 10vol.% concentration, one had the significant tolerance and showed a relative growth yield of 86% for acetone, 71% for methanol, 52% for benzene, 35% for heptane, 24% for toluene and 19% for ethylacetate. the phylogenetic analysis of t ...201121388656
[the diversity of alkane degrading bacteria in the enrichments with deep sea sediment of the south china sea].deep sea sediment samples of the south sea of china were used for isolation and biodiversity examination of hydrocarbon degrading bacterium. 48 isolates were obtained from the enrichments with hexadecane as the sole carbon sources. among them, 27 isolates were capable of degrading alkane; and 4 could produce biosurfactant significantly as determined by the surface tension measurement. 2 isolates belonging to dietzia maris lowered water surface tension to 33 mn/m. this is the first report about d ...200718062265
draft whole-genome sequence of the type strain bacillus aquimaris tf12t.bacillus aquimaris tf12 is a gram-positive bacteria isolated from a tidal flat of the yellow sea in south korea. we report the draft whole-genome sequence of bacillus aquimaris tf12, the type strain of a set of bacteria typically associated with marine habitats and with a potentially high biotechnology value.201627417832
screening of pigmented bacillus aquimaris sh6 from the intestinal tracts of shrimp to develop a novel feed supplement for shrimp.to develop a novel feed supplement for shrimp using pigmented spore-forming bacterial strains isolated from their gastrointestinal tracts.201627548588
induction of invertebrate larval settlement; different bacteria, different mechanisms?recruitment via settlement of pelagic larvae is critical for the persistence of benthic marine populations. for many benthic invertebrates, larval settlement occurs in response to surface microbial films. larvae of the serpulid polychaete hydroides elegans can be induced to settle by single bacterial species. until now, only pseudoalteromonas luteoviolacea had been subjected to detailed genetic and mechanistic studies. to determine if the complex structures, termed tailocins, derived from phage- ...201728195220
raw starch-degrading α-amylase from bacillus aquimaris mksc 6.2: isolation and expression of the gene, bioinformatics and biochemical characterization of the recombinant enzyme.the aims were to isolate a raw starch-degrading α-amylase gene baqa from bacillus aquimaris mksc 6.2, and to characterize the gene product through in silico study and its expression in escherichia coli.201323020612
nitrate treatment effects on bacterial community biofilm formed on carbon steel in produced water stirred tank bioreactor.to better understand the impact of nitrate in brazilian oil reservoirs under souring processes and corrosion, the goal of this study was to analyse the effect of nitrate on bacterial biofilms formed on carbon steel coupons using reactors containing produced water from a brazilian oil platform. three independent experiments were carried out (e1, e2 and e3) using the same experimental conditions and different incubation times (5, 45 and 80 days, respectively). in every experiment, two biofilm-reac ...201222806109
bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains l-lysine in its cell wall.a halotolerant, round-endospore-forming, aerobic, gram-positive bacterium, designated bh724(t), was isolated from a solar saltern at taean in korea. cells of this strain were rod-shaped and found to be non-motile. strain bh724(t) grew at salinities of 0-10 % (w/v) nacl with an optimum of 3 % (w/v) nacl and at temperatures of 15-50 degrees c with an optimum of 40 degrees c. phylogenetic analyses based on 16s rrna gene sequences showed that strain bh724(t) belonged to the genus bacillus and that b ...200616902027
stability and detergent compatibility of a predominantly β-sheet serine protease from halotolerant b. aquimaris vitp4 strain.the present study deals with the characterization of halotolerant protease produced by bacillus aquimaris vitp4 strain isolated from kumta coast, karnataka, india. the studies were performed at 40 °c and ph 8 in tris buffer. metal ions such as mn(2+) and ca(2+) increased the proteolytic activity of the enzyme by 34 and 30 %, respectively, at 10 mm concentration. cu(2+) at 1 mm concentration was found to enhance the enzyme activity by 16 %, whereas inhibition was observed at higher concentration ...201424114320
isolation and characterization of a metal ion-dependent alkaline protease from a halotolerant bacillus aquimaris vitp4.a halotolerant bacterium bacillus acquimaris vitp4 was used for the production of extracellular protease. fractional precipitation using ammonium chloride was used to obtain the enzyme. the protease exhibited optimum activity at ph 8.0 and 40 degrees c and retained 50% of its optimal proteolytic activity even in the presence of 4 m nacl, suggesting that it is halotolerant. the molecular mass of protease, as revealed by sds-page was found to be 34 kda and the homogeneity of the enzyme was confirm ...201121682140
bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the cuatro ciénegas valley in coahuila, mexico.a moderately halophilic, gram-positive and rod-shaped bacterium, strain m4-4t, was isolated from a chihuahuan desert lagoon in cuatro ciénegas, coahuila, mexico. strain m4-4t was found to grow optimally at 30-37 degrees c, ph 7.0-8.0 and 5 % nacl and to tolerate from 0.5 % to 10 % nacl. it was shown to be aerobic. the genomic dna g+c content was about 37 mol%. strain m4-4t exhibited minimal or no growth on most sugars tested. its major cellular fatty acids were c14 : 0, c16 : 0 and c18 : 1. base ...200818398195
bacillus marisflavi sp. nov. and bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the yellow sea in korea.two gram-positive or gram-variable, endospore-forming, moderately halophilic rods (strains tf-11(t) and tf-12(t)) were isolated from a tidal flat of the yellow sea in korea and were subjected to a polyphasic taxonomic study. strains tf-11(t) and tf-12(t) had cell-wall peptidoglycan based on meso-diaminopimelic acid. the predominant menaquinone found in the two strains was mk-7. the cellular fatty acid profiles of both strains contained large amounts of branched and saturated fatty acids. the maj ...200313130010
experimental evidence of chemical defence mechanisms in antarctic bryozoans.bryozoans are among the most abundant and diverse members of the antarctic benthos, however the role of bioactive metabolites in ecological interactions has been scarcely studied. to extend our knowledge about the chemical ecology of antarctic bryozoans, crude ether extracts (ee) and butanol extracts (be) obtained from two antarctic common species (cornucopina pectogemma and nematoflustra flagellata), were tested for antibacterial and repellent activities. the extracts were screened for quorum q ...201728487162
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