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ageratum enation virus-a begomovirus of weeds with the potential to infect crops.samples of two ageratum conyzoides, one sonchus oleraceus and one turnip (brassica rapa var. rapa) exhibiting virus-like symptoms were collected from pakistan and nepal. full-length begomovirus clones were obtained from the four plant samples and betasatellite clones from three of these. the begomovirus sequences were shown to be isolates of ageratum enation virus (aev) with greater than 89.1% nucleotide sequence identity to the 26 aev sequences available in the databases. the three betasatellit ...201525674770
βc1 is a pathogenicity determinant: not only for begomoviruses but also for a mastrevirus.βc1 proteins, encoded by betasatellites, are known to be pathogenicity determinants, and they are responsible for symptom expression in many devastating diseases caused by begomoviruses. we report the involvement of βc1 in pathogenicity determination of a mastrevirus. analysis of field samples of wheat plants containing wheat dwarf india virus (wdiv) revealed the presence of a full-length and several defective betasatellite molecules. the detected betasatellite was identified as ageratum yellow ...201425000899
virus-induced gene silencing using a modified betasatellite: a potential candidate for functional genomics of crops.betasatellites are geminivirus-associated single-stranded dna molecules that play an important role in symptom modulation. a vigs vector was developed by modifying cotton leaf curl multan betasatellite (clcumb). clcumb dna was modified by replacing the βc1 gene with a multiple cloning site. the silencing ability of the modified clcumb was investigated by cloning a fragment of a host gene (su) or a reporter transgene (uida) into the modified clcumb and co-agroinoculation with cotton leaf curl mul ...201424610555
molecular characterization and pathogenicity of a carrot (daucus carota) infecting begomovirus and associated betasatellite from india.the yellow mosaic pattern and shortening of leaf petiole are common disease symptoms associated with begomovirus infection in carrot. dna from field infected carrot leaves was analyzed by rolling circle amplification and sequencing. the results established the presence of ageratum enation virus (aev), which is referred to here as ageratum enation virus-carrot (aev-car). symptomatic ageratum (ageratum conyzoides) plants, growing adjacent to the carrot fields, also showed the presence of aev (aev- ...201324512752
molecular identification of ageratum enation virus, betasatellite and alphasatellite molecules isolated from yellow vein diseased amaranthus cruentus in india.natural occurrence of yellow vein disease on amaranthus cruentus was observed at lucknow, india in the year 2008. the causal virus was successfully transmitted through whiteflies (bemisia tabaci) from diseased a. cruentus to healthy seedlings of a. cruentus and other test species which indicated begomovirus infection. the begomovirus dna-a, betasatellite, and alphasatellite components associated with yellow vein disease were amplified by rolling circle amplification using ø-29 dna polymerase fro ...201323963765
changes in the metabolome and histopathology of amaranthus hypochondriacus l. in response to ageratum enation virus infection.amaranthus hypochondriacus l. infected with ageratum enation virus (aev) was investigated for identifying alteration in the anatomical structures, sap translocation and metabolomic variations using light microscopy, magnetic resonance imaging, nmr spectroscopy and gc-ms, respectively. combination of gc-ms and nmr spectroscopy identified 68 polar and non-polar metabolites that were present in different levels in healthy and virus-infected a. hypochondriacus. contrast of t₁ and t₂ weighted mr imag ...201222683210
ageratum enation virus infection induces programmed cell death and alters metabolite biosynthesis in papaver somniferum.a previously unknown disease which causes severe vein thickening and inward leaf curl was observed in a number of opium poppy (papaver somniferum l.) plants. the sequence analysis of full-length viral genome and associated betasatellite reveals the occurrence of ageratum enation virus (aev) and ageratum leaf curl betasatellite (alcb), respectively. co-infiltration of cloned agroinfectious dnas of aev and alcb induces the leaf curl and vein thickening symptoms as were observed naturally. infectiv ...201728729873
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