| assembly of g1 and g2 glycoprotein oligomers in punta toro virus-infected cells. | we have studied the oligomerization of the membrane glycoproteins of punta toro virus (ptv), a member of the phlebovirus genus of the family bunyaviridae, and the effect of glycosylation on protein stability and transport. by using sucrose gradient centrifugation, the g1 and g2 glycoproteins in ptv-infected or recombinant-transfected cells were found to sediment as dimers after dsp cross-linking, suggesting that the g 1 and g2 proteins are associated as dimers by non-covalent interactions. pulse ... | 1992 | 1320791 |
| effect of macrophage source and activation on susceptibility in an age-dependent model of murine hepatitis caused by a phlebovirus, punta toro. | the adames strain of a bunyavirus, punta toro virus (ptv), is an hepatotrophic virus that has been described to produce an age-dependent lethal hepatic necrosis in 3-4 week old c57bl/6 mice, but 8 week old mice survive with minimal necrosis. the course of ptv infection in vitro in macrophages derived from these mice served as a model to study the pathogenesis of phlebovirus infection. peripheral blood monocytes, resident or elicited peritoneal macrophages, and kupffer cell liver macrophages, as ... | 1992 | 1370368 |
| potential role of immunomodulators for treatment of phlebovirus infections of animals. | rift valley fever (rvfv) is a major phlebovirus-induced epizootic disease of domestic animals (primarily cattle and sheep) in africa. no therapies for the disease are known. a related phlebovirus, punta toro virus (ptv), has been adapted to induce an rvfv-like disease in c57bl/6 mice. this ptv infection has been used as a model for rvfv because it is reasonably safe and does not require high-level biologic containment. the infection model has been used to study the potential role of immunomodula ... | 1992 | 1385686 |
| effect of human, recombinant interleukin 2 on punta toro virus infections in c57bl/6 mice. | the effect of human recombinant interleukin-2 (ril-2) on punta toro virus (ptv) infection was investigated in c57bl/6 mice. immunologic and viral parameters were assessed after mice were treated i.p. with ril-2 for 5 days. treatment of mice with 25000 and 12500 units/mouse of ril-2 resulted in significant inhibition of the disease as indicated by increases in survival of mice as well as decreases in liver and serum virus titers. serum glutamic oxalic acid and pyruvic acid transaminase levels wer ... | 1991 | 1719936 |
| assembly and polarized release of punta toro virus and effects of brefeldin a. | punta toro virus (ptv), a member of the sandfly fever group of bunyaviruses, is assembled by budding at intracellular membranes of the golgi complex. we have examined ptv glycoprotein transport, assembly, and release and the effects of brefeldin a (bfa) on these processes. both the g1 and g2 proteins were transported out of the endoplasmic reticulum (er) and retained in the golgi complex in a stable structure, either during ptv infection or when expressed from a vaccinia virus recombinant. bfa t ... | 1991 | 1847462 |
| role of hepatocytes and kupffer cells in age-dependent murine hepatitis caused by a phlebovirus, punta toro. | punta toro virus (ptv) infection of c57bl/6 mice results in fulminant hepatic necrosis and death in 3-week-old susceptible mice, but survival with minimal hepatocellular necrosis in 8-week-old resistant mice. susceptibility in 3-week-old mice is associated with an earlier rise of viral titers in liver and serum than that occurring in 8-week-old resistant mice. there is also an earlier and more rapid accumulation of infectious progeny in serum vs. liver after ptv infection in both age groups, sug ... | 1991 | 1901906 |
| golgi complex localization of the punta toro virus g2 protein requires its association with the g1 protein. | the glycoproteins of bunyaviruses accumulate in membranes of the golgi complex, where virus maturation occurs by budding. in this study we have constructed a series of full length or truncated mutants of the g2 glycoprotein of punta toro virus (ptv), a member of the phlebovirus genus of the bunyaviridae, and investigated their transport properties. the results indicate that the hydrophobic domain preceding the g2 glycoprotein can function as a translocational signal peptide, and that the hydroph ... | 1991 | 1905078 |
| pathogenesis of a phleboviral infection (punta toro virus) in golden syrian hamsters. | the hamster, mesocricetus auratus, was examined as a possible model for investigating the poorly defined pathogenesis of the family bunyaviridae, genus phlebovirus. punta toro virus (ptv) isolates from eastern panama were highly virulent for two outbred and five inbred hamster strains, while isolates from western panama were of low virulence. the adames strain (eastern panama) of ptv (ld50 approximately 1 pfu, sc) caused an acute fatal disease (average survival time, 3.8 days) in 10-week-old lak ... | 1990 | 2122875 |
| antigenic analysis of punta toro virus and identification of protective determinants with monoclonal antibodies. | hybridomas producing monoclonal antibodies to the three major structural proteins of punta toro virus (ptv) were established by fusion of spleen cells with sp2/0-ag-14 mouse plasmacytoma cells. thirty-six independently derived monoclonal antibodies were evaluated in neutralization, hemagglutination inhibition, and elisa assays and the isotype, antigen specificities, and cross-reactivities were determined. these antibodies were also assessed for their ability to provide protection in a murine mod ... | 1988 | 2462308 |
| antiviral and cytotoxicity evaluation of 3-nitro-3-deazauridine. | 3-nitro-3-deazauridine (3n-3du) is a new synthetic nucleoside having activity against members of 5 rna virus families including: paramyxoviruses (parainfluenza, piv), picornaviruses (rhino-, rv), rhabdoviruses (vesicular stomatitis, vsv), togaviruses (semliki forest, sfv) and bunyaviruses (punta toro, ptv). in this report, we evaluate and compare its activity with the parent nucleoside, 3-deazauridine (3du) and ribavirin as drug standards. comparison of drug activities utilizes observations of a ... | 1989 | 2634363 |
| mechanism of selection of class ii recombinant murine leukemia viruses in the highly leukemic strain cwd. | the development of spontaneous lymphomas in cwd mice is associated with the expression of endogenous ecotropic murine leukemia viruses (mulv) and the formation of recombinant viruses. however, the pattern of substitution of nonecotropic sequences within the envelope genes of the cwd class ii recombinant viruses differs from that seen in class i recombinant mulvs of akr, c58, and hrs mice. to determine how cwd host genes might influence the envelope gene structure of the recombinant viruses, we c ... | 1988 | 2831378 |
| effects of ribamidine, a 3-carboxamidine derivative of ribavirin, on experimentally induced phlebovirus infections. | ribamidine (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamidine) was inhibitory in rhesus monkey kidney (llc-mk2 derivative) cells to adames and balliet strains of punta toro virus (ptv), a phlebovirus related to rift valley fever and sandfly fever viruses. the 50% effective dose was 8 and 12 micrograms/ml against each respective virus strain; the 50% cytotoxic dose was 320 micrograms/ml, giving selectivity indices of 40 and 27 against each virus strain. the virus ratings were 1.2 and 1.0, res ... | 1988 | 2976263 |
| in vitro and in vivo phlebovirus inhibition by ribavirin. | ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) was markedly inhibitory in vitro to adames and balliet strains of punta toro virus (ptv), a phlebovirus related to rift valley fever and sandfly fever viruses. by using inhibition of viral cytopathic effect in llc-mk2 cells with both virus strains, the 50% effective dose was 4 to 10 micrograms/ml and the virus rating was 1.3. the adames strain of ptv infection in mice was established for evaluation of the in vivo antiviral efficacy ... | 1988 | 3129991 |
| intracellular accumulation of punta toro virus glycoproteins expressed from cloned cdna. | the punta toro virus (ptv) middle size (m) rna encodes two glycoproteins, g1 and g2, and possibly a nonstructural protein, nsm. a partial cdna clone of the m segment which contains g1 and g2 glycoprotein coding sequences but lacks most of the nsm sequences was inserted into the genome of vaccinia virus under the control of an early vaccinia promoter. cells infected with the recombinant virus were found to synthesize two polypeptides with molecular weights of 65,000 (g1) and 55,000 (g2) that reac ... | 1988 | 3142146 |
| [electrical properties of the foot-and-mouth disease virus]. | it has been shown that the electron microscopy method can be used for characteristics of the electric properties of foot-and-mouth disease virus. the appearance of simultaneous positive and negative staining during the negative staining of virus preparations with 3-4% ptv solution shows the presence of full virions of constant poles designated as positively and negatively stained areas of protein coat surface. the lateral orientation of virions on the film at routine conditions of preparation an ... | 1984 | 6087939 |
| a single dominant gene determines susceptibility to a leukaemogenic recombinant retrovirus. | the production of recombinant retroviruses is an important episode in the natural history of thymic leukaemia in akr and hrs/j (hr/hr) mices. these viruses apparently originate from ecotropic and xenotropic precursors in the late preleukaemic stage of the disease. analyses of their structural proteins and genomic oligonucleotides indicate that they arise by recombination of env genes of the precursor viruses. this event leads to a viral envelope glycoprotein (gp70) with some peptides that have f ... | 1981 | 7254316 |
| antiviral activity of an immunomodulatory lipophilic desmuramyl dipeptide analog. | bch-527, the lipophilic hydrochloride salt of octadecyl d-alanyl l-glutamine, was evaluated for efficacy against experimentally induced murine cytomegalovirus (mcmv), influenza a (h1n1) (iv-a), and punta toro virus (ptv) infections in mice. the compound was administered i.p. every other day for a total of 4 injections commencing 24 h previrus exposure. doses ranged from 12.5 to 200 mg/kg per injection in the various experiments. the mcmv infection was significantly inhibited in two experiments b ... | 1995 | 7605113 |
| antiviral and immunomodulating inhibitors of experimentally-induced punta toro virus infections. | a major component of a us army medical research and development command-supported program to discover and develop new drugs for the treatment of rift valley fever, sandfly fever, and crimean-congo hemorrhagic fever has been to study candidate test materials against hepatotropic infections of c57bl/6 mice induced by the related but less biohazardous punta toro virus (ptv). the effects of 75 compounds, some of which were considered immunomodulators in their primary mechanism of activity, were stud ... | 1994 | 7847873 |
| antiviral activities of tragacanthin polysaccharides on punta toro virus infections in mice. | tragacanthin polysaccharides from astragalus brachycentrus (av208) and astragalus echidnaeformis (av212) plants, which are devoid of in vitro antiviral activity, were evaluated in a mouse model of punta toro virus (ptv) infection. the ptv (a phlebovirus member of the bunyaviridae family of viruses) is a model for studying the treatment of rift valley fever and hantavirus infections. single intraperitoneal treatments with 12.5-200 mg/kg/day doses of av212 given 24 h before or 4 and 24 h after vir ... | 1996 | 8804797 |
| molecular bases of tropism in the pur46 cluster of transmissible gastroenteritis coronaviruses. | transmissible gastroenteritis coronavirus (tgev) infects both, the enteric and the respiratory tract of swine. s protein, that is recognized by the cellular receptor, has been proposed that plays an essential role in controlling the dominant tropism. the genetic relationship of s gene from different enteric strains and non-enteropathogenic porcine respiratory coronaviruses (prcvs) was determined. a correlation between tropism and the genetic structure of the s gene was established. prcvs, derive ... | 1995 | 8830541 |
| enhanced cellular immunity to hepatitis c virus nonstructural proteins by codelivery of granulocyte macrophage-colony stimulating factor gene in intramuscular dna immunization. | hepatitis c virus (hcv) nonstructural (ns) proteins appeared to be important targets for hcv vaccine development, since ns-specific t-helper-cell responses are associated with clearance from acute hcv infection. in this report, we have constructed a plasmid, ptv-ns345, that encodes the hcv ns3, ns4 and ns5 proteins (ns345) and a bicistronic plasmid, ptv-ns345/gmcsf, in which the hcv ns345 polyprotein and gmcsf are translated independently. intramuscular inoculation with ptv-ns345 plasmid dna int ... | 1999 | 10195625 |
| self-inactivating lentiviral vectors with u3 and u5 modifications. | lentiviral vectors have gained much attention in recent years mainly because they integrate into nondividing host-cell genomes. for clinical applications, a safe and efficient lentiviral vector system is required. previously, we have established a human immunodeficiency virus type 1 (hiv-1)-derived three-plasmid lentiviral vector system for viral vector production which includes a packaging vector php, a transducing vector ptv, and an envelope-encoding plasmid phef-vsvg. cotransfection of these ... | 1999 | 10441560 |
| efficacy and safety analyses of a recombinant human immunodeficiency virus type 1 derived vector system. | lentiviruses infect both dividing and nondividing cells. in this study we characterized a lentiviral vector system consisting of a packaging vector (php) and a transducing vector (ptv) derived from a recombinant human immunodeficiency virus type 1 (hiv-1). in php, the long terminal repeats (ltrs), the 5' untranslated leader and portions of the env and nef genes were deleted. the leader sequence of php was substituted with a modified rous sarcoma virus (rsv) 59 bp leader containing a mutated rsv ... | 1999 | 10505094 |
| porcine teschoviruses comprise at least eleven distinct serotypes: molecular and evolutionary aspects. | nucleotide sequencing and phylogenetic analysis of 10 recognized prototype strains of the porcine enterovirus (pev) cytopathic effect (cpe) group i reveals a close relationship of the viral genomes to the previously sequenced strain f65, supporting the concept of a reclassification of this virus group into a new picornavirus genus. also, nucleotide sequences of the polyprotein-encoding genome region or the p1 region of 28 historic strains and recent field isolates were determined. the data sugge ... | 2001 | 11160660 |
| detection of porcine teschovirus and enterovirus type ii by reverse transcription-polymerase chain reaction. | porcine enteroviruses (pevs) have been recognized as the causative agents of various clinical manifestations such as fertility disorders, neurological defects, and dermal lesions in pigs. currently, the diagnosis of pev infection is carried out by virus isolation, which although useful, is labor- and time-intensive. the present investigation describes the development of a reverse transcription-polymerase chain reaction (rt-pcr) assay for the rapid and sensitive detection of pevs of cytopathic ef ... | 2002 | 12423029 |
| dosimetric and technical considerations for interstitial adenoviral gene therapy as applied to prostate cancer. | to introduce a simple and specific dosimetric model and an adenoviral delivery technique for interstitial adenoviral gene therapy of prostate cancer. | 2003 | 12504055 |
| molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in peru. | peru is a centre of origin and domestication of the potato, pepper and tomato (family solanaceae). many potyviruses (genus potyvirus) that infect these crops were described 20-30 years ago. however, definitive classification of these viruses as distinct species remains unresolved for several reasons, including their close serological relationships, similar symptomatology in test plants and lack of genomic sequence data. using samples collected from peru, we have determined the complete genomic s ... | 2003 | 12917478 |
| functional and structural similarities between the internal ribosome entry sites of hepatitis c virus and porcine teschovirus, a picornavirus. | initiation of protein synthesis on picornavirus rna requires an internal ribosome entry site (ires). typically, picornavirus ires elements contain about 450 nucleotides (nt) and use most of the cellular translation initiation factors. however, it is now shown that just 280 nt of the porcine teschovirus type 1 talfan (ptv-1) 5' untranslated region direct the efficient internal initiation of translation in vitro and within cells. in toeprinting assays, assembly of 48s preinitiation complexes from ... | 2004 | 15078929 |
| strains of peru tomato virus infecting cocona (solanum sessiliflorum), tomato and pepper in peru with reference to genome evolution in genus potyvirus. | two isolates (sl1 and sl6) of peru tomato virus (ptv, genus potyvirus) were obtained from cocona plants (solanum sessiliflorum) growing in tingo maría, the jungle of the amazon basin in peru. one ptv isolate (tm) was isolated from a tomato plant (lycopersicon esculentum) growing in huaral at the peruvian coast. the three ptv isolates were readily transmissible by myzus persicae. isolate sl1, but not sl6, caused chlorotic lesions in inoculated leaves of chenopodium amaranticolor and c. quinoa. is ... | 2004 | 15290372 |
| apoptosis of hepatocytes caused by punta toro virus (bunyaviridae: phlebovirus) and its implication for phlebovirus pathogenesis. | experimental infection of hamsters with punta toro virus (ptv) produces a disease with clinical and pathological similarities to the severe human hemorrhagic fever caused by rift valley fever virus (rvfv), thus providing an animal model for rvfv pathogenesis. in this model, hepatocytic apoptosis is the main pathological component of liver injuries that are responsible for severe hemorrhagic manifestations. to further elucidate whether viral replication in hepatocytes directly causes apoptosis, w ... | 2005 | 16192639 |
| functional analyses of rna structures shared between the internal ribosome entry sites of hepatitis c virus and the picornavirus porcine teschovirus 1 talfan. | the internal ribosome entry site (ires) of porcine teschovirus 1 (ptv-1), a member of the picornaviridae family, is quite distinct from other well-characterized picornavirus ires elements, but it displays functional similarities to the ires from hepatitis c virus (hcv), a member of the flaviviridae family. in particular, a dominant negative mutant form of eif4a does not inhibit the activity of the ptv-1 ires. furthermore, there is a high level (ca. 50%) of identity between the ptv-1 and hcv ires ... | 2006 | 16415004 |
| protective immunity against acute phleboviral infection elicited through immunostimulatory cationic liposome-dna complexes. | cationic liposome-dna complexes (cldc) have been demonstrated to induce potent antitumor activities. the ability of these complexes to elicit protective immunity against viral infections has not been fully explored. here we report findings on the use of cldc as an antiviral agent in a mouse model of acute phleboviral (punta toro virus) disease. cldc treatment of mice challenged with punta toro virus (ptv) resulted in dramatic increases in survival and reduced viral burden and other parameters in ... | 2006 | 16430975 |
| [liver damage caused by hepatitis c viral infection and ethyl alcohol consumption]. | hepatitis c virus infection (hcv) is a complex disease, most commonly chronicle (80-85%). the aim of this research was to determinate the level of the liver damage in the patients cansed by hcv in conjunction with consuming ethyl alcohol. | 2006 | 16683409 |
| recombinant eimeria protozoan protein elicits resistance to acute phlebovirus infection in mice but not hamsters. | a protein antigen from an eimeria protozoan has recently been reported to induce antitumor activity in mice. this activity most likely results from the strong induction of interkeukin-12 (il-12) and gamma interferon (ifn-gamma), which are also essential factors in the establishment of protective immunity against viral infection. we evaluated recombinant eimeria antigen (rea) as a potential immunotherapeutic agent in mouse and hamster models of acute phleboviral disease. punta toro virus (ptv) wa ... | 2006 | 16723561 |
| the s segment of punta toro virus (bunyaviridae, phlebovirus) is a major determinant of lethality in the syrian hamster and codes for a type i interferon antagonist. | two strains of punta toro virus (ptv), isolated from febrile humans in panama, cause a differential pathogenesis in syrian hamsters, which could be a useful model for understanding the virulence characteristics and differential outcomes in other phleboviral infections such as rift valley fever virus. genetic reassortants produced between the lethal adames (a/a/a) and nonlethal balliet (b/b/b) strains were used in this study to investigate viral genetic determinants for pathogenesis and lethality ... | 2007 | 17050607 |
| tlr3 deletion limits mortality and disease severity due to phlebovirus infection. | tlr3 was the first member of the tlr family of pattern recognition receptors found to detect a conserved viral molecular pattern, dsrna, yet supporting evidence for a major role in host defense against viral pathogens is limited. punta toro virus (ptv) has been shown to produce severe infection in mice, modeling disease caused by the related highly pathogenic rift valley fever phlebovirus in humans and domesticated ungulates. using tlr3-deficient mice, we investigated the involvement of tlr3 in ... | 2006 | 17056560 |
| tlr3 is essential for the induction of protective immunity against punta toro virus infection by the double-stranded rna (dsrna), poly(i:c12u), but not poly(i:c): differential recognition of synthetic dsrna molecules. | in the wake of rna virus infections, dsrna intermediates are often generated. these viral pathogen-associated molecular patterns can be sensed by a growing number of host cell cytosolic proteins and tlr3, which contribute to the induction of antiviral defenses. recent evidence indicates that melanoma differentiation-associated gene-5 is the prominent host component mediating ifn production after exposure to the dsrna analog, poly(i:c). we have previously reported that punta toro virus (ptv) infe ... | 2007 | 17404303 |
| isolation and molecular characterization of a porcine teschovirus 1 isolate from china. | porcine teschovirus 1 (ptv-1) (swine/ch/imh/03) was isolated from piglets in a farm in inner mongolia province, p.r. china. it was confirmed by electron microscopy, rt-pcr, and sequencing. comparison of the sequences of the amino acid and nucleotides and phylogenetic analysis of the polyprotein showed that ptv swine/ch/imh/03 strain is ptv-1. the isolated virus has closest relationship with talfan strain, they shared 98.9% and 99.5% homology of amino acids and nucleotides, respectively, in the o ... | 2007 | 17432938 |
| the picornavirus avian encephalomyelitis virus possesses a hepatitis c virus-like internal ribosome entry site element. | avian encephalomyelitis virus (aev) is a picornavirus that causes disease in poultry worldwide, and flocks must be vaccinated for protection. aev is currently classified within the hepatovirus genus, since its proteins are most closely related to those of hepatitis a virus (hav). we now provide evidence that the 494-nucleotide-long 5' untranslated region of the aev genome contains an internal ribosome entry site (ires) element that functions efficiently in vitro and in mammalian cells. unlike th ... | 2008 | 18077729 |
| expression of heterologous genes in oncolytic adenoviruses using picornaviral 2a sequences that trigger ribosome skipping. | insertion of picornaviral 2a sequences into mrnas causes ribosomes to skip formation of a peptide bond at the junction of the 2a and downstream sequences, leading to the production of two proteins from a single open reading frame. adenoviral protein ix is a minor capsid protein that has been used to display foreign peptides on the surface of the capsid. we have used 2a sequences from the foot-and-mouth disease virus (fmdv) and porcine teschovirus 1 (ptv-1) to express protein ix (pix) and green f ... | 2008 | 18198369 |
| prophylactic and therapeutic intervention of punta toro virus (phlebovirus, bunyaviridae) infection in hamsters with interferon alfacon-1. | punta toro virus (ptv) is a member of the bunyaviridae family, genus phlebovirus, related to the highly pathogenic rift valley fever virus (rvfv). it produces a disease in hamsters that models severe rift valley fever (rvf) in humans. the recent outbreak of rvf in kenya stresses the need to identify prophylactic and therapeutic measures for preventing and treating severe forms of disease. to this end, interferon (ifn) alfacon-1 (consensus ifn-alpha) was evaluated in cell culture against rvfv and ... | 2008 | 18222548 |
| immunoprophylaxis of punta toro virus (phlebovirus, bunyaviridae) infection in hamsters with recombinant eimeria profilin-like antigen. | recombinant eimeria antigen (rea) has been shown to have potent anticancer and antiviral activity in respective mouse disease models, presumably through robust immune stimulation that occurs via tlr11, a pattern recognition receptor that recognizes profilin-like proteins expressed on apicomplexan protozoans. comparable immunostimulatory activity in other species has yet to be demonstrated. since rea is known to be highly effective in treating punta toro virus (ptv) infection in mice, its ability ... | 2008 | 18550012 |
| characterization of a new potyvirus infecting pepper crops in ecuador. | sequencing 2,951 nucleotides of the 3' proximal region of the genome of a potyvirus isolate collected from capsicum pubescens (rocoto) pepper in ecuador revealed that this was the first representative of a new species tentatively named ecuadorian rocoto virus (erv). phylogeny reconstruction showed that this isolate clustered with potato virus v (pvv), peru tomato virus and wild potato mosaic virus into a monophyletic group, and was closest to pvv. the isolate was shown to be infectious in tobacc ... | 2008 | 18553171 |
| characterization of cell-death pathways in punta toro virus-induced hepatocyte injury. | punta toro virus (ptv; genus phlebovirus, family bunyaviridae) causes apoptosis of hepatocytes in vivo in experimentally infected hamsters and in vitro in cultured hepg2 cells. screening for expression of apoptosis-related genes has shown alterations in the genes for tumour necrosis factor-alpha (tnf-alpha) and the tnf receptor family. this study examined the roles of the tnf receptor-related extrinsic pathway and the bcl-2 family-associated mitochondrial pathway in ptv-induced cell death. the e ... | 2008 | 18753227 |
| prophylaxis with cationic liposome-dna complexes protects hamsters from phleboviral disease: importance of liposomal delivery and cpg motifs. | cationic liposome-dna complexes (cldc) are cationic/neutral lipid carriers complexed with plasmid dna that when administered systemically results in a robust t(h)1 cytokine response. cldc have been shown to be effective in prophylaxis and therapeutic treatment of animal models of viral disease. to determine the contribution of liposomal delivery and cpg content of the plasmid dna to the efficacy of cldc; plasmid, cpg-free plasmid dna, or cpg-containing oligodeoxynucleotides (odn) with and withou ... | 2009 | 18840471 |
| the complete nucleotide sequence of potato virus t. | potato virus t (pvt), a member of an unassigned species in the family flexiviridae, has a genome 6,539 nt in size with three orfs coding for replication-associated proteins (185 kda, orf 1), movement protein (40 kda, orf 2) and coat protein (24 kda, orf 3), respectively. pvt differs from the type members of all genera of the family flexiviridae with a 30k-type movement protein and is phylogenetically distant from all of these viruses, least so from grapevine virus a (gva, genus vitivirus), with ... | 2009 | 19115033 |
| conserved functional domains and a novel tertiary interaction near the pseudoknot drive translational activity of hepatitis c virus and hepatitis c virus-like internal ribosome entry sites. | the translational activity of the hepatitis c virus (hcv) internal ribosome entry site (ires) and other hcv-like ires rnas depends on structured rna elements in domains ii and iii, which serve to recruit the ribosomal 40s subunit, eukaryotic initiation factor (eif) 3 and the ternary eif2/met-trna(i)(met)/gtp complex and subsequently domain ii assists subunit joining. porcine teschovirus-1 talfan (ptv-1) is a member of the picornaviridae family, with a predicted hcv-like secondary structure, but ... | 2009 | 19596815 |
| punta toro virus (bunyaviridae, phlebovirus) infection in mice: strain differences in pathogenesis and host interferon response. | the adames strain of punta toro virus (ptv-a, bunyaviridae, phlebovirus) causes an acute lethal disease in hamsters and mice. the balliet strain of the virus (ptv-b) is generally considered to be avirulent. the difference in hamster susceptibility is likely due to the ability of ptv-a to suppress interferon (ifn)-beta similarly to that described for rift valley fever virus. here we investigated strain differences in ptv pathogenesis and the ifn response in mice. although ptv-b infection in mice ... | 2009 | 19783024 |
| efficacy of favipiravir (t-705) and t-1106 pyrazine derivatives in phlebovirus disease models. | several studies have reported favipiravir (t-705) to be effective in treating a number of viral diseases modeled in rodent systems. notably, the related pyrazine derivative, t-1106, was found to be more effective than t-705 in treating yellow fever virus infection in hamsters. based on these findings, we hypothesized that t-1106 may be more effective in treating hepatotropic punta toro virus (ptv, phlebovirus) infection in rodents. in cell culture, the inhibitory concentrations of the compounds ... | 2010 | 19874853 |
| non-structural and nucleocapsid proteins of punta toro virus induce apoptosis of hepatocytes through both intrinsic and extrinsic pathways. | punta toro virus (ptv; family bunyaviridae, genus phlebovirus) causes severe hepatic damage through brisk apoptosis of hepatocytes. in the present study, two viral proteins encoded by the s segment of the viral genome, non-structural (nss) and nucleocapsid protein (n), were examined for their roles in apoptosis. expression of nss in hepg2 cells led to apoptosis in 45% of transfected cells, and with n, 28%, on average. these levels represent a four- to an eightfold increase over cells transfected ... | 2010 | 20055939 |
| influence of the hepatitis c virus 3'-untranslated region on ires-dependent and cap-dependent translation initiation. | translation of hepatitis c virus (hcv) genomic rna is directed by an internal ribosome entry site (ires) in the 5'-untranslated region (5'-utr), and the hcv 3'-utr enhances ires activity. since the hcv 3'-utr has a unique structure among 3'-utrs, we checked possible communication between the 5'- and the 3'-utr of hcv during translation using chimeric reporter rnas. we show that translation directed by the hcv ires and by the hcv-like ires of porcine teschovirus (ptv) which belongs to a quite dis ... | 2010 | 20079737 |
| isolation and characterization of the first chinese strain of porcine teschovirus-8. | investigations were carried out to identify the causal agent of acute diarrhea, respiratory distress, and death of pigs on a swine farm in jilin province, northern china. only porcine teschovirus (ptv, designated as ptv-8 jilin/2003) was isolated from samples of organs. the presence of ptv was confirmed by the production of a specific cytopathic effect on susceptible cells and by the results of the immunoperoxidase monolayer assay (ipma), polymerase chain reaction, and electron microscopy. other ... | 2010 | 20362007 |
| genotyping of porcine teschovirus from nervous tissue of pigs with and without polioencephalomyelitis in indiana. | porcine teschovirus (ptv) was isolated in cell culture and/or demonstrated by polymerase chain reaction in samples of brain and/or spinal cord in pigs in indiana during the 2002-2007 period. testing was initiated on pigs originating from populations exhibiting nervous clinical disease and/or pigs with microscopic lesions in central nervous tissues, indicating viral encephalitis and/or myelitis. virus was demonstrated in pigs with and without lesions as well as with and without nervous clinical d ... | 2010 | 20622231 |
| a multiplex rt-pcr for rapid and simultaneous detection of porcine teschovirus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. | a multiplex rt-pcr (mrt-pcr) assay was developed and evaluated for its ability to detect multiple viral infections of swine simultaneously. one pair of primers was selected carefully for each of the following three rna viruses: porcine reproductive and respiratory syndrome virus (prrsv), classical swine fever virus (csfv), and porcine teschovirus (ptv). each target produced a specific amplicon with a size of 451bp (prrsv), 343bp (csfv), or 163bp (ptv). the sensitivity of the mrt-pcr using purifi ... | 2010 | 21192983 |
| host genetic variation in susceptibility to punta toro virus. | infection of small laboratory animals by punta toro virus (ptv), family bunyaviridae, genus phlebovirus, is a model for the study of the human pathogen rift valley fever virus (rvfv). we have identified inbred mouse strains with significant differences in host response to the adames strain of ptv. nine inbred strains of mice representing major branches in the mus musculus phylogeny were inoculated subcutaneously with a high dose of ptv in survival experiments. two inbred strains of mice, nzw/lac ... | 2011 | 21320557 |
| Duck Hepatitis A Virus Possesses a Distinct Type IV Internal Ribosome Entry Site Element of Picornavirus. | Sequence analysis of duck hepatitis virus type 1 (DHV-1) led to its classification as the only member of a new genus, Avihepatovirus, of the family Picornaviridae, and so was renamed duck hepatitis A virus (DHAV). The 5' untranslated region (5' UTR) plays an important role in translation initiation and RNA synthesis of the picornavirus. Here, we provide evidence that the 651-nucleotide (nt)-long 5' UTR of DHAV genome contains an internal ribosome entry site (IRES) element that functions efficien ... | 2012 | 22090106 |
| teschoviruses and sapeloviruses in faecal samples from wild boar in spain. | teschovirus and sapelovirus are two genera of the picornaviridae family, comprising highly variable and heterogeneous enteric viruses, commonly found in faecal samples from domestic pigs. although both of them are also known to infect wild boar, studies on their presence in these wild suids are scarce. the present study aimed at determining the presence of porcine teschovirus (ptv) and sapelovirus (psv) in free-living wild boar populations, as well as to study their relationships with similar vi ... | 2012 | 23265238 |
| development of real-time reverse transcriptase qpcr assays for the detection of punta toro virus and pichinde virus. | research with high biocontainment pathogens such as rift valley fever virus (rvfv) and lassa virus (lasv) is expensive, potentially hazardous, and limited to select institutions. surrogate pathogens such as punta toro virus (ptv) for rvfv infection and pichinde virus (picv) for lasv infection allow research to be performed under more permissive bsl-2 conditions. although used as infection models, ptv and picv have no standard real-time rt-qpcr assays to detect and quantify pathogenesis. ptv is a ... | 2016 | 27029488 |
| phleboviruses and the type i interferon response. | the genus phlebovirus of the family bunyaviridae contains a number of emerging virus species which pose a threat to both human and animal health. most prominent members include rift valley fever virus (rvfv), sandfly fever naples virus (sfnv), sandfly fever sicilian virus (sfsv), toscana virus (tosv), punta toro virus (ptv), and the two new members severe fever with thrombocytopenia syndrome virus (sftsv) and heartland virus (hrtv). the nonstructural protein nss is well established as the main p ... | 2016 | 27338447 |
| the survey of porcine teschoviruses in field samples in china with a universal rapid probe real-time rt-pcr assay. | a real-time reverse transcription polymerase chain reaction (rt-pcr) based on taqman was established and evaluated for quantitative detection of porcine teschoviruses (ptvs). a pair of primers and a taqman probe targeting on the highly conserved sequence of the 5'-untranslated region (5'-utr) of one to 11 serotypes of ptv were designed. standard plasmid dna containing pcr amplification of the 5'-utr were constructed and used to develop the real-time rt-pcr. the results indicated that the real-ti ... | 2013 | 23138413 |
| the survey of porcine teschoviruses, sapeloviruses and enteroviruses b infecting domestic pigs and wild boars in the czech republic between 2005 and 2011. | this study presents results of epidemiological survey and genetic characterisation of porcine enteric picornaviruses belonging to the genera teschovirus, sapelovirus, and porcine enterovirus b. faecal or gut content samples from domestic pigs (sus scrofa f. domestica) and the cecal content of wild boars (sus scrofa) of different ages (collected between 2005 and 2011) were analysed by molecular methods. porcine enterovirus b was the most prevalent virus detected in both domestic pigs and wild boa ... | 2012 | 22579481 |
| epidemiological survey of enteric viruses in wild boars in the czech republic: first evidence of close relationship between wild boar and human rotavirus a strains. | population of wild boar is increasing in the whole europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. the aim of the study was to estimate in population of free-living wild boar in the czech republic the prevalence of enteric viral pathogens, namely rotavirus groups a and c (rva and rvc), porcine reproductive and respiratory syndrome virus (prrsv), and members of family coronavirid ... | 2016 | 27599927 |
| [new classification of enteroviruses of pigs: serological and molecular genetic aspects]. | due to changes in modern classification and nomenclature family picornaviridae made by the international committee of taxonomy of viruses, conducted genetic and serological reclassification of 36 industrial, typical and laboratory strains porcine enteroviruses isolated in ukraine. the results of molecular genetic studies of 34 strains assigned to the family picornaviridae, genus teschovirus, species porcine teschovirus, 2 strains of virus did not engage in polymerase chain reaction with species ... | 2014 | 25199345 |
| rearrangement of influenza virus spliced segments for the development of live-attenuated vaccines. | influenza viral infections represent a serious public health problem, with influenza virus causing a contagious respiratory disease which is most effectively prevented through vaccination. segments 7 (m) and 8 (ns) of the influenza virus genome encode mrna transcripts that are alternatively spliced to express two different viral proteins. this study describes the generation, using reverse genetics, of three different recombinant influenza a/puerto rico/8/1934 (pr8) h1n1 viruses containing m or n ... | 2016 | 27122587 |
| real-world safety and efficacy of ombitasvir/paritaprevir/ritonavir/+dasabuvir±ribavirin (obv/ptv/r/+dsv±rbv) therapy in recurrent hepatitis c virus (hcv) genotype 1 infection post-liver transplant: amber-cee study. | background the introduction of direct-acting antivirals (daas) has considerably improved therapeutic outcomes for patients with chronic hepatitis c virus (hcv) infections. the amber-cee study aimed to assess real-world efficacy and safety of ombitasvir/paritaprevir/ritonavir/+ dasabuvir ±ribavirin (obv/ptv/r/ +dsv±rbv) in the treatment of post-transplant recurrence of hcv infection. material and methods liver transplant recipients with recurrent hcv genotype 1 infection, scheduled for obv/ptv/r/ ... | 2017 | 28386057 |
| potential liver transplant recipients with hepatitis c: should they be treated before or after transplantation? | treatment of hepatitis c virus (hcv) with newer directly acting antivirals (daas) and lead to sustained viral response (svr) in majority of patients and svr has been documented to be associated with reversal of liver cirrhosis. the improved svr rates and safety profiles of daas have led to the treatment of patients with decompensated cirrhosis awaiting liver transplantation (lt). several clinical trials of daas in decompensated hcv patients have recently demonstrated svr rates above 80%, which h ... | 2017 | 28348470 |
| real-world experience with the all-oral, interferon-free regimen of ombitasvir/paritaprevir/ritonavir and dasabuvir for the treatment of chronic hepatitis c virus infection in the german hepatitis c registry. | real-world studies are relevant to complement clinical trials on novel antiviral therapies against chronic hepatitis c, however clinical practice data are currently limited. this study investigated effectiveness and safety of ombitasvir/paritaprevir/ritonavir (obv/ptv/r) ± dasabuvir (dsv) ± ribavirin (rbv) for treatment of hcv genotype (gt) 1 and gt4 infection in a large real-world cohort. the german hepatitis c registry is an observational cohort study prospectively collecting clinical practice ... | 2017 | 28342229 |
| turquoise-i part 1b: ombitasvir/paritaprevir/ritonavir and dasabuvir with ribavirin for hepatitis c virus infection in hiv-1 coinfected patients on darunavir. | ombitasvir/paritaprevir/ritonavir with dasabuvir (obv/ptv/r + dsv) ± ribavirin (rbv) is approved for hepatitis c virus (hcv) genotype 1 (gt1) treatment in hiv-1 coinfected patients. in healthy controls, coadministration of obv/ptv/r + dsv + darunavir (drv) lowered drv trough concentration (ctrough) levels. to assess the clinical significance of this change, turquoise-i, part 1b, evaluated the efficacy and safety of obv/ptv/r + dsv + rbv in coinfected patients on stable, drv-containing antiretrov ... | 2017 | 28329334 |
| optimizing hepatitis c virus treatment through pharmacist interventions: identification and management of drug-drug interactions. | to quantify drug-drug-interactions (ddis) encountered in patients prescribed hepatitis c virus (hcv) treatment, the interventions made, and the time spent in this process. | 2017 | 28321163 |
| effectiveness, safety and clinical outcomes of direct-acting antiviral therapy in hcv genotype 1 infection: results from a spanish real-world cohort. | clinical trials evaluating second-generation direct-acting antiviral agents (daas) have shown excellent rates of sustained virologic response (svr) and good safety profiles in patients with chronic hepatitis c virus (hcv) genotype 1 infection. we aimed to investigate the effectiveness and safety of two oral daa combination regimens, ombitasvir/paritaprevir/ritonavir plus dasabuvir (omv/ptv/r+dsv) and ledipasvir/sofosbuvir (ldv/sof), in a real-world clinical practice. | 2017 | 28189751 |
| combination therapy with ombitasvir/paritaprevir/ritonavir for dialysis patients infected with hepatitis c virus: a prospective multi-institutional study. | hepatitis c virus (hcv) infection is common in dialysis patients worldwide and nosocomial hcv spread within dialysis facilities continues to develop. combination therapy with daclatasvir and asunaprevir (dcv/asv) that has proven efficacy for dialysis patients infected with genotype 1b hcv (hcv/1b) has several concerns in japan. the recently available combination therapy with ombitasvir, paritaprevir, and ritonavir (obv/ptv/r) is not contraindicated in patients with chronic renal failure and has ... | 2017 | 28090038 |
| ombitasvir/paritaprevir/ritonavir and dasabuvir combination treatment in patients with hiv/hcv co-infection: results of an italian compassionate use program. | patients co-infected with hepatitis c virus (hcv) and human immunodeficiency virus (hiv) are at high risk of liver disease progression. we report a favorable safety profile and svr12 rates of 96.7% among hiv/hcv co-infected patients participating in an italian compassionate-use program of ombitasvir/paritaprevir/ritonavir + dasabuvir (obv/ptv/r + dsv) ± ribavirin (rbv). | 2016 | 28011605 |
| therapy with ombitasvir/paritaprevir/ritonavir plus dasabuvir is effective and safe for the treatment of genotypes 1 and 4 hepatitis c virus (hcv) infection in patients with severe renal impairment: a multicentre experience. | limited data are available on direct-acting antivirals for treating hepatitis c virus (hcv) infection in patients with severe renal impairment. the aim of this study was to evaluate the effectiveness and safety of ombitasvir/paritaprevir/ritonavir (obv/ptv/r) ± dasabuvir (dsv) ± ribavirin (rbv) in patients with stage 4 or 5 chronic kidney disease (ckd) and hcv genotype 1 or 4 infection in real clinical practice, and to investigate pharmacological interactions. this retrospective study included p ... | 2016 | 27976490 |
| time to viral suppression is not related to achievement of svr12 in hcv gt1-infected patients treated with ombitasvir/paritaprevir/ritonavir and dasabuvir with or without ribavirin. | high rates of sustained virologic response at post-treatment week 12 (svr12) were achieved in six phase 3 trials of ombitasvir (obv, an ns5a inhibitor), paritaprevir (an ns3/4a protease inhibitor) co-dosed with ritonavir (ptv/r) + dasabuvir (dsv, an ns5b rna polymerase inhibitor) (ie, 3d regimen) with or without ribavirin (rbv) in adults with chronic genotype (gt) 1 hepatitis c virus (hcv) infection. we assessed whether time to first hcv rna value below the lower limit of quantification in patie ... | 2017 | 27935166 |
| evaluation of a potential interaction between new regimens to treat hepatitis c and warfarin. | new regimens to treat hepatitis c virus infection have expanded the eligible patient population to include more patients receiving concurrent warfarin. the primary objective of this study was to assess whether a drug interaction occurs when these regimens are added to warfarin therapy. | 2016 | 27465881 |
| effectiveness and safety of ombitasvir-paritaprevir/ritonavir and dasabuvir with or without ribavirin for hcv genotype 1 infection for 12 weeks under routine clinical practice. | no previous studies exist examining the effectiveness and safety in real clinical practice of the combination of ombitasvir/paritaprevir/ritonavir and dasabuvir (obv/ptv/r+dsv). | 2016 | 27422641 |
| cost-effectiveness of direct-acting antiviral regimen ombitasvir/paritaprevir/ritonavir in treatment-naïve and treatment-experienced patients infected with chronic hepatitis c virus genotype 1b in japan. | this study compared the cost-effectiveness of chronic hepatitis c virus (hcv) genotype 1b (gt1b) therapy ombitasvir/paritaprevir/ritonavir (obv/ptv/r) vs daclatasvir + asunaprevir (dcv/asv) and no treatment in patients without cirrhosis. cost-effectiveness analyses (ceas) that compared obv/ptv/r against dcv/asv and sofosbuvir/ledipasvir (sof/ldv) in y93h mutation-negative, gt1b patients with and without cirrhosis were also included. | 2016 | 27348464 |
| economic evaluation of ombitasvir/paritaprevir/ritonavir and dasabuvir for the treatment of chronic genotype 1 hepatitis c virus infection. | to estimate clinical outcomes and cost-effectiveness of ombitasvir/paritaprevir/ritonavir and dasabuvir ± ribavirin (omb/ptv/r + dsv ± rbv) compared with treatment regimens including pegylated interferon (pegifn) for patients with chronic genotype 1 hepatitis c virus (hcv) infection. | 2016 | 27172133 |
| safety and efficacy of ombitasvir/paritaprevir/ritonavir plus dasabuvir with or without ribavirin in hcv-infected patients taking concomitant acid-reducing agents. | acid-reducing agents (aras) and proton-pump inhibitors (ppis) that increase gastric ph can alter the bioavailability of antiviral drugs, particularly relevant in patients with advanced liver disease caused by chronic hepatitis c virus (hcv) infection seeking therapy. using integrated data from six phase 3 studies, we report the safety and efficacy of the 3-direct-acting antiviral (daa) regimen containing ombitasvir (obv, an ns5a inhibitor), ritonavir-boosted paritaprevir (ptv/r, an ns3/4a protea ... | 2016 | 27045929 |
| efficacy and safety of ombitasvir/paritaprevir/r and dasabuvir compared to ifn-containing regimens in genotype 1 hcv patients: the malachite-i/ii trials. | telaprevir plus pegylated interferon/ribavirin (tpv+pegifn/rbv) remains a therapeutic option for chronic hepatitis c virus (hcv) genotype (gt) 1 infection in many regions. we conducted two open-label, phase iiib trials comparing safety and efficacy of all-oral ombitasvir/paritaprevir/ritonavir and dasabuvir±ribavirin (obv/ptv/r+dsv±rbv) and tpv+pegifn/rbv. | 2016 | 26321288 |
| randomized phase 3 trial of ombitasvir/paritaprevir/ritonavir for hepatitis c virus genotype 1b-infected japanese patients with or without cirrhosis. | gift-i is a phase 3 trial evaluating the efficacy and safety of a 12-week regimen of coformulated ombitasvir (obv)/paritaprevir (ptv)/ritonavir (r) for treatment of japanese hepatitis c virus genotype 1b-infected patients. it consists of a double-blind, placebo-controlled substudy of patients without cirrhosis and an open-label substudy of patients with compensated cirrhosis. patients without cirrhosis were randomized 2:1 to once-daily obv/ptv/r (25 mg/150 mg/100 mg; group a) or placebo (group b ... | 2015 | 26147154 |
| drug-drug interaction profile of the all-oral anti-hepatitis c virus regimen of paritaprevir/ritonavir, ombitasvir, and dasabuvir. | paritaprevir (administered with ritonavir, ptv/r), ombitasvir (obv), and dasabuvir (dsv) are direct-acting antiviral agents (daas) for the treatment of chronic hepatitis c virus (hcv) infection. thirteen studies were conducted to characterize drug-drug interactions for the 3d regimen of obv, ptv/r, and dsv and various medications in healthy volunteers to inform dosing recommendations in hcv-infected patients. | 2015 | 25646891 |
| survival of porcine teschovirus as a surrogate virus on pork chops during storage at 2°c. | due to the lack of an efficient cultivation system, little is known about the stability and inactivation of hepatitis e virus (hev). in addition, there is a lack of information on which cultivable virus(es) are suitable as model or surrogate viruses for hev. murine norovirus (mnv) and f-rna coliphage ms2 are potential surrogates and f-rna coliphages are a potential indicator for enteric viruses. however, the numbers of f-rna coliphages excreted by swine are relatively low. in contrast, porcine t ... | 2015 | 25461604 |
| extended protection against phlebovirus infection conferred by recombinant adenovirus expressing consensus interferon (def201). | punta toro virus (ptv; bunyaviridae, phlebovirus) is related to rift valley fever virus (rvfv), a pathogenic agent which causes severe disease in humans and livestock primarily in the sub-saharan region of africa. the recent range expansion of rvfv and the potential for its intentional release into naïve populations pose a significant threat to public health and agriculture. studies modeling disease in rodents and nonhuman primates have shown that ptv and rvfv are highly sensitive to the antivir ... | 2012 | 22615273 |
| a manual and an automatic ters based virus discrimination. | rapid techniques for virus identification are more relevant today than ever. conventional virus detection and identification strategies generally rest upon various microbiological methods and genomic approaches, which are not suited for the analysis of single virus particles. in contrast, the highly sensitive spectroscopic technique tip-enhanced raman spectroscopy (ters) allows the characterisation of biological nano-structures like virions on a single-particle level. in this study, the feasibil ... | 2015 | 25686406 |
| failure patterns and survival in patients with nasopharyngeal carcinoma treated with intensity modulated radiation in northwest china: a pilot study. | to evaluate the clinical outcomes and patterns of failure in patients with nasopharyngeal carcinoma (npc) treated with intensity modulated radiotherapy (imrt) in northwest china. | 2012 | 22233756 |
| radiation dermatitis caused by a bolus effect from an abdominal compression device. | american association of physicists in medicine (aapm) task group 176 evaluated the dosimetric effects caused by couch tops and immobilization devices. the report analyzed the extensive physics-based literature on couch tops, stereotactic body radiation therapy (sbrt) frames, and body immobilization bags, while noting the scarcity of clinical reports of skin toxicity because of external devices. here, we present a clinical case report of grade 1 abdominal skin toxicity owing to an abdominal compr ... | 2016 | 27264694 |
| an unusual case of eyelid metastasis from a rectal primary. | a 26-year-old, human immunodeficiency virus. (hiv).negative male, a diagnosed case of locally advanced adenocarcinoma rectum underwent diversion colostomy and received preoperative radiotherapy with three-dimensional conformal radiotherapy. (3dcrt) to a dose of 25 gy/5 fx/8. days to 95% planning target volume. (ptv) with 3-field technique. (right and left lateral and posterior) by 6 and 15 mv photons. postradiotherapy contrast-enhanced computed tomography. (cect) chest and abdomen showed progres ... | 2016 | 26881622 |
| complete genome sequence of pepper yellow mosaic virus, a potyvirus, occurring in brazil. | the complete genomic sequence of pepper yellow mosaic virus (pepymv), a member of the genus potyvirus, was determined. the sequence was 9745 nucleotides long, excluding the 3' poly(a) tail. the genome contained a large open reading frame encoding a polyprotein of 3085 amino acids, which contained the typically conserved motifs found in members of the genus potyvirus and an additional p3-pipo (pretty interesting potyvirus orf). in a pairwise comparison with other potyvirus sequences, the full gen ... | 2012 | 22527869 |
| f-coliphages, porcine adenovirus and porcine teschovirus as potential indicator viruses of fecal contamination for pork carcass processing. | there are concerns about the zoonotic transmission of viruses through undercooked pork products. there is a lack of information on suitable indicator viruses for fecal contamination with pathogenic enteric viruses in the meat processing chain. the study compared the incidence and levels of contamination of hog carcasses with f-coliphages, porcine teschovirus (ptv), and porcine adenovirus (padv) at different stages of the dressing process to assess their potential as indicator viruses of fecal co ... | 2017 | 27810445 |
| significant symptoms alleviation and tumor volume reduction after combined simultaneously integrated inner-escalated boost and volumetric-modulated arc radiotherapy in a patient with unresectable bulky hepatocellular carcinoma: a care-compliant case report. | clinically, elderly patients with unresectable bulky hepatocellular carcinoma (hcc) are difficult to manage, especially in those with co-infections of hepatitis b and c virus. herein, we reported such a case treated with radiotherapy (rt) by using combined simultaneously integrated inner-escalated boost and volumetric-modulated arc radiotherapy (sieb-vmat). after rt, significant symptoms alleviation and durable tumor control were observed. | 2016 | 27559982 |
| analysis of full-length genomes of porcine teschovirus (ptv) and the effect of purifying selection on phylogenetic trees. | to study the outcome of natural selection using phylogenetic trees, we analyzed full-length genome sequences of porcine teschovirus (ptv). ptv belongs to the family picornaviridae and has a positive-stranded rna genome, the replication of which is carried out by the error-prone viral rna-dependent rna polymerase. the viral rna encodes a single polyprotein that is cleaved into structural (i.e., l, vp4, vp2, vp3 and vp1) and nonstructural proteins (i.e., 2a, 2b, 2c, 3a, 3b, and 3c). a high degree ... | 2016 | 26860913 |
| characterization of the punta toro species complex (genus phlebovirus, family bunyaviridae). | punta toro virus (ptv), a member of the ptv complex, is a relatively common causative agent of febrile illness in panama that is often misdiagnosed as 'dengue' or 'influenza'. currently, only two named members make up this species complex, ptv and buenaventura virus (buev). genomic and antigenic characterization of 17 members of the ptv complex, nine of which were isolated from human acute febrile illness cases, reveals that this species complex is composed of six distant viruses. we propose to ... | 2015 | 25934793 |
| comparison of zetaplus 60s and nitrocellulose membrane filters for the simultaneous concentration of f-rna coliphages, porcine teschovirus and porcine adenovirus from river water. | increasing attention is being paid to the impact of agricultural activities on water quality to understand the impact on public health. f-rna coliphages have been proposed as viral indicators of fecal contamination while porcine teschovirus (ptv) and porcine adenovirus (padv) are proposed indicators of fecal contamination of swine origin. viruses and coliphages are present in water in very low concentrations and must be concentrated to permit their detection. there is little information comparin ... | 2014 | 24880068 |
| experimental teschovirus encephalomyelitis in gnotobiotic pigs. | a central nervous system (cns) disorder characterized by non-suppurative encephalomyelitis with neurological signs was induced experimentally in gnotobiotic pigs by intravenous and oral or intranasal inoculation of the porcine teschovirus (ptv) toyama 2002 strain isolated from breeding pigs in japan. lesions consisting of perivascular cuffing of mononuclear cells, focal gliosis, neuronal necrosis and neuronophagia were observed in the brainstem, cerebellum and spinal cord. non-suppurative gangli ... | 2014 | 24650890 |
| the survey of porcine teschoviruses, porcine circovirus and porcine transmissible gastroenteritis virus infecting piglets in clinical specimens in china. | a multiplex pcr assay was developed and evaluated for its ability to simultaneously detect three viral infections of swine. specific primers were carefully selected from articles published for each of the following three viruses: porcine circovirus type ii (pcv2), porcine teschovirus (ptv) and porcine transmissible gastroenteritis virus (tgev). each target produced a specific amplicon with a size of 353 bp (pcv2), 168 bp (ptv) and 499 bp (tgev). the sensitivity of the multiplex pcr using purifie ... | 2013 | 23224864 |