| the use of an automated assay to assess phage survival after a biocidal treatment. | the coliphage k1-5 has been used in an automated assay to monitor the viricidal activity of various disinfectants. this indirect assay based on the spectrophotometric reading of the lysis of the host cell (escherichia coli d837) produced encouraging results and was faster than the overlay counting method (previously studied) which relies on plaque formation. however, differences in sensitivity towards some disinfectants were observed between the two methods. | 1996 | 8698661 |
| bacteriophage k1-5 encodes two different tail fiber proteins, allowing it to infect and replicate on both k1 and k5 strains of escherichia coli. | a virulent double-stranded dna bacteriophage, phi k1-5, has been isolated and found to be capable of infecting escherichia coli strains that possess either the k1 or the k5 polysaccharide capsule. electron micrographs show that the virion consists of a small icosohedral head with short tail spikes, similar to members of the podoviridae family. dna sequence analysis of the region encoding the tail fiber protein showed two open reading frames encoding previously characterized hydrolytic phage tail ... | 2001 | 11222673 |
| the genome of bacteriophage k1f, a t7-like phage that has acquired the ability to replicate on k1 strains of escherichia coli. | bacteriophage k1f specifically infects escherichia coli strains that produce the k1 polysaccharide capsule. like several other k1 capsule-specific phages, k1f encodes an endo-neuraminidase (endosialidase) that is part of the tail structure which allows the phage to recognize and degrade the polysaccharide capsule. the complete nucleotide sequence of the k1f genome reveals that it is closely related to bacteriophage t7 in both genome organization and sequence similarity. the most striking differe ... | 2005 | 16321955 |