immunofluorescence, bacteriological and patho-morphological studies of the organs of respiration of children who died of acute pneumonia. 19751919
associated seroconversions to respiratory viruses in volunteers with experimental influenza infection.serological examinations of 573 volunteers with mild experimental influenza infection and 86 volunteers of a control group hospitalized in a special clinic revealed a significant rise in the titre of antibodies (seroconversion) not only to influenza a or b viruses used for the experimental infection but in 23.3 to 29.8% of cases also to other respiratory viruses. based on a number of arguments, associated seroconversions are interpreted as due to mixed or sequential infections of different aetio ...19765868
effect of streptovirudin on parainfluenza 3 virus multiplication.streptovirudin (sv) in a concentration of 100 microng/ml reduced the multiplication of parainfluenza 3 virus by about 3.5 log units. when uninfected wish cells were treated for 2 and 12 hours with 100 mug/ml of sv, 3h-thymidineincorporation was decreased, but no marked differences were observed in the incorporation of 3h-uridine. the level of intracellular cyclic adenosine monophosphate (c-amp) was found to decrease in sv-treated cells.197715435
exacerbations of chronic bronchitis: exogenous or endogenous infection?six male patients with chronic bronchitis, who were known previously to have excreted streptococcus pneumoniae and/or haemophilus influenzae, both at the times of exacerbations and during remission, were studied for 43 to 52 months. sputum was examined fortnightly and at the time of exacerbations. strains of strep. pneumoniae were serotyped and those of haemophilus species were typed by antibiograms along with other supporting methods. sera collected before or at the time of an exacerbation and ...197825664
haemagglutination inhibition antibodies in nasal secretions of persons after natural parainfluenza virus 7 adults and 7 children with upper respiratory illness of parainfluenza aetiology, the virus was isolated in the acute phase from nasal washings or nasal smears in spite of high titres of haemagglutination inhibition (hi) antibodies in serum. by contrast, secretory hi antibodies were not demonstrated at the onset of illness in any of the patients, but their formation started early and the antibodies reached maximal levels about 10 days after onset of illness. the individual patients showed co ...197827967
morphological characteristics of acute respiratory tract diseases of mixed etiology in children.the analysis of 30 etiologically deciphered (by the direct coons method) autopsy observations of acute respiratory diseases in children under 8 is presented. monoinfections were established only in 6 cases. morphological examinations of respiratory organs in 24 cases of mixed acute respiratory diseases (associations of virus-bacteria and virus-mycoplasma pneumoniae-diplostreptococcus) revealed marked circulatory disorders and desquamative, dystrophic, and inflammatory processes in segmental bron ...197828114
viruses and bacteria associated with acute respiratory illnesses in young children in general practice.the results obtained and the laboratory methods used for the isolation of viruses and bacteria from malaysian children with acute respiratory illnesses seen in a private clinic are described. of the 65 children studied virus isolations were obtained from 26 children, bacteria from 10 and both virus and bacteria from another 5. the agents isolated were influenza viruses, parainfluenza viruses, adenoviruses, bordetella pertussis, streptococcus pneumoniae, haemophilus influenzae and staphylococcus ...197830173
effect of microwaves on cell function and virus replication in cell cultures irradiated in vitro. 197546732
immunologic and virologic aspects of secretory immune system in human respiratory tract.many external mucosal surfaces in man are replete with immunoglobulin containing plasma cells and thymic dependent (t) lymphocytes. immunization with viral vaccines administered via different routes have indicated that viral specific secretory immunoglobulins particularly of gamma a class are synthesized locally in the external mucosal surfaces. local availability of viral antigens especially after local mucosal immunization appears to be the most effective means of inducing viral specific secre ...197547823
studies on parainfluenza-3 virus neuraminidase.various parainfluenza-3 virus (piv-3) strains differ in neuraminidase (na) activity, some being neuraminidase strong (nas) and some neuraminidase weak (naw). no difference in the ability of these strains to elicit antibody activity to hemagglutinin (ha) or na of piv-3 was observed. samples of formalin-treated piv-3 released more n-acteylneuraminic acid (nana) than samples of piv-3 not treated with formalin when these samples were incubated with the substrate sialolactose for 16 h at 37 degrees c ...197547826
letter: cellular immunity in multiple sclerosis. 197548155
lactoglobulins (goat) in the immunofluorescence staining of parainfluenza virus type 2 in l-132 cells.instillation of parainfluenza virus type 2 in the mammary gland of the goat resulted in the appearance of specific and high titered antibodies in the milk. the globulin fraction of the milk was separated and conjugated with fluorescein isothiocyanate. these conjugated antibodies could be successfully used in the specific staining of the homologous virus in infected l-132 cells. therefore, milk from such immunized animals represents a rich and convenient source of specific antibodies for use in i ...197549216
letter: lymphocyte transformation in vitro to paramyxovirus antigens in multiple sclerosis. 197553681
letter: cellular immunity in multiple sclerosis. 197655641
[comparative study of the complement-binding activity of antigens from para-influenza viruses of types 1, 2 and 3 on continuous hela cells]. 197656484
glycoproteins of sendai virus: purification and antigenic analysis.solubilized envelope antigens (glycoproteins) of sendai virus were purified in a deae-bio-gel a column. monospecific antisera were prepared against the antigens, designated hn and f glycoproteins. these glycoproteins are antigenically distinct. immunodiffusion analyses of the envelope antigens of three representative paramyxoviruses (sendai, yucaipa, and newcastle disease virus)did not reveal any cross-reactions among them with antisera specific for sendai virus glycoproteins.197558850
[thomsen antigen--a new receptor arising in cells under the influence of myxoviruses and several bacteria]. 197662465
skin hypersensitivity to parainfluenza-3 virus in cattle. ii. induction by vaccination and specificity. 197665073
persistent sv5 virus infection in continuous cell cultures.a continuous line of guinea pig kidney cells (cgpk/h) and a continuous line of mouse fibroblasts (l/h) spontaneously infected with parainfluenza virus sv5 were found. these cultures showed no enhanced cell degeneration or symplast formation, nor was haemagglutinin accumulation or infectious virus demonstrated in them. however, regular reproduction of ribonucleoprotein (rnp) characteristic of parainfluenza viruses, morphologically complete virions and antigens producing antibody to sv5 virus were ...197665119
coxsackie virus infection in acute myocardial infarction.of 38 patients with a clinical diagnosis of acute myocardial infarction 10 had serologial evidence of recent coxsackie b virus infection.197767289
serological response of multiple sclerosis patients and controls to 6/94-parainfluenza virus.the serological responses of 195 multiple sclerosis (ms) patients and 251 controls were tested against 6/94-parainfluenza virus, which was previously isolated from brain tissue of two patients with ms. the hemagglutination-inhibition titers of greater than or equal to 1: 128 were found more frequently in ms patients (21.5%) than in controls (14.0%). however, the geometric mean titers did not differ between these two groups. the present study concludes that a causal relationship of 6/94-virus to ...197772793
serological relationships between certain avian and animal avian paramyxovirus designated robin/hiddensee/19/75 proved to be related to the yucaipa virus group in cross haemagglutination inhibition test (hit). whereas the prototype parainfluenza viruses did not react with the antiserum raised against the robin virus, a newly isolated bovine parainfluenza 3 virus was shown to be inhibited up to half the homologous titre. possible antigenic relationships between avian, mammalian and human paramyxoviruses are discussed.197774944
contamination of primary embryonic bovine kidney cell cultures with parainfluenza type 2 simian virus 5 and infectious bovine rhinotracheitis virus.two different viruses were isolated from bovine embryonic cell cultures after two subcultures from the primary cells. one virus was identified as parainfluenza type 2 simian virus 5 (sv-5), and the other was identified as infectious bovine rhinotracheitis virus. six months later, stock cultures of pig kidney (pk-15) cells were found to be contaminated with sv-5 virus. we believe that the source of the sv-5 virus in the bovine cells was a cross-contamination from monkey kidneys during preparation ...197875889
epidemiology of respiratory viral infection among paediatric inpatients over a six-year period in north-east england.during a 6-year, hospital-based study at newcastle upon tyne five consecutive winter epidemics of respiratory syncytial (r.s.) virus infection occurred; the virus was identified in 1428 cases, showing that 1 in 50 live-births were admitted to hospital with r.s. virus infection. epidemics were inversely related to temperature and to number of hours of sunshine. parainfluenzaviruses, the second largest group of pathogens, were identified in 543 cases; most infections by this group were due to para ...197882045
male oscuro--r.s.v.? 197985878
[persistent infection in murine l cells infected with the parainfluenza virus sv5].persistent infection of mouse l cells was achieved by serial passages of undiluted parainfluenza sv5 virus. at the level of the 10--12th passage a stationary noncytocidal type of persistent infection was established. a low level of the infectious virus produced by the persistently infected cells was observed; the number of cells synthesizing the virus-specific antigen detectable by immunofluorescence was high. persistently infected cells were resistant to the infection with the homologous virus ...197988818
etiological and clinical investigation of bronchiolitis. 197895930
evaluation of five temperature-sensitive mutants of respiratory syncytial virus in primates: ii. genetic analysis of virus recovered during infection.five temperature-sensitive (ts) mutants of respiratory syncytial (rs) virus (ts-1, ts-1 ng-1, ts-1 ng-16, ts-2, and ts-7), previously evaluated forinfectivity and virulence in chimpanzees and owl monkeys, were also assayed for in vivo genetic stability. none of the five mutants tested was completely stable genetically. thus, virus which had lost some or all of the ts property was recovered from each infected chimpanzee. significantly, each ts-1 ng-1 isolate retained some degree of temperature se ...1978104003
evaluation of five temperature-sensitive mutants of respiratory syncytial virus in primates: i. viral shedding, immunologic response, and associated illness. 1978104004
[role of heterogenized cellular antigens in cross immunity reactions in paramyxovirus infections].heterogenized antigens similar to the antigens of sheep and guinea pig red blood cells were revealed by indirect immunofluorescence in tissue cultures infected with parotitis virus. participation of these antigens in cross immunofluorescence reactions observed in tissue cultures infected with various paramyxoviruses and in a suspension of erythrocytes loaded with these viruses was established. it was shown that immunization of children with parotitis virus was accompanied by a specific anamnesti ...1978106603
respiratory syncytial virus infection in owl monkeys: viral shedding, immunological response, and associated illness caused by wild-type virus and two temperature-sensitive mutants.intranasal inoculation of owl monkeys with wild-type respiratory syncytial virus induced upper respiratory tract disease in each of seven animals. the response of owl monkeys to two highly defective, temperature-sensitive, multiple-lesion mutants was then compared to the pattern seen with wild-type respiratory syncytial virus. these mutants, ts-1 ng-1 and ts-1 ng-16, were derived from the ts-1 mutant that had been remutagenized with nitrosoguanidine (ng). previously the ts-1 ng-1 and ts-1 ng-16 ...1979118925
the longitudinal approach to the pathogenesis of respiratory disease.longitudinal observations were made of a well-defined population of children at a day care center in an investigation of the pathogenesis of infections due to respiratory syncytial virus (rsv) and mycoplasma pneumoniae. a single rsv infection induced a modest but significant degree of resistance to further rsv infection in these children. age and immunity seemed to interact to decrease the intensity of the clinical expression of illness associated with rsv infection. infants and young children h ...1979121783
[specific antibodies in the nasal secretum of children with rs viral affection of lower respiratory pathways (author's transl)]. 1979161514
inhibition of respiratory syncytial, parainfluenza 3 and measles viruses by 2-deoxy-d-glucose. 1975163047
role of respiratory viruses in childhood mortality.respiratory viruses have been identified at necropsy in the lungs of 13 out of 24 children who died with observed acute respiratory illness. the histological appearances of the lungs supported the association between virus and death in each of these 13 children and suggested an unidentified virus aetiology in a further five cases. histological appearances compatible with bacterial infection were found in the lungs of only two of the 24 children. similar virus and histological findings have been ...1975163114
further observations on the characteristics of a bovine parainfluenza-3 variant.calves were inoculated with a bovine para-influenza-3 variant to determine its pathogenicity and the stability of its cytopathic feature and its inability to agglutinate chicken erythrocytes. the inoculated calves and one contact animal developed an immune response without significant clinical illness. the clinical response in calves was similar to that induced by the parent virus strain. the variant was shown to retain its characteristic cytopathic effect for madin darby bovine kidney cells and ...1975163127
immunologic significance of the mumps virus skin test in infants, children and adults.the biologic validity of cell-mediated immunity to mumps virus was evaluated in 395 children, adolescents and adults. the study protocol included the determination of cutaneous delayed hypersensitivity to viral and avian control antigens and in 79% of the subjects an essential double bleeding was performed before and after mumps virus skin test for assay of neutralizing antibody. seven per cent of subjects expressed sufficient delayed hypersentitivity to the control antigen to erase an apparentl ...1975163575
the use of vaccines in respiratory virus infections. 1975163955
serologic response of lambs to live virus vaccines containing infectious bovine rhinotracheitis, bovine virus diarrhea and bovine myxovirus parainfluenza-3. 1975164079
viral infection and psychiatric disorders.a psychiatric population of 94 inpatients and 12 outpatients was investigated on referral to a psychiatric unit in a general hospital for serum antibody titres to several viruses by a complement fixation technique. of the total population studied, only eight were considered to have antibody titres of possible significance. this result would appear to indicate that viral infection does not play a major part in the causation or precipitation of psychiatric disorders.1975164099
[influence of calves' age on a program of intranasal vaccination against infectious bovine rhinotracheitis and para-influenza 3]. 1975164272
bovine parainfluenza type 3 virus infection: virus replication in bovine embryonic cell cultures and virion separation by rate-zonal centrifugation.replicative sequences of a bovine strain of parainfluenza type 3 virus in bovine embryonic kidney and spleen cell cultures were investigated by light and fluorescence microscopy and by ultrathin section and negative-contrast electron microscopy. observations from light and fluorescence microscopy showed that intracytoplasmic inclusions were detected as small granules surrounding the nuclei of more than 90 percent of the cell population by day 2 postinoculation. with the increase of postexposure ...1975164407
bovine parainfluenza type 3 virus infection: ultrastructural aspects of viral pathogenesis in the bovine respiratory tract.after aerosolization of a bovine strain of parainfluenza type 3 virus, the pathogenesis of the virus was followed from the trachea to the bronchioalveolar compartments of the lung of colostrum-free calves and of conventionally reared calves during a 5- to 12-day postexposure interval. by tissue titration, plaque assay, and electron microscopy, it was found that virus infection could be established in colostrum-free calves as well as in conventionally reared calves, even though sequential change ...1975164408
detection of cellular hypersensitivity among multiple sclerosis (ms) patients to 6/94 virus; a parainfluenza type 1 isolate from ms brain tissue.multiple sclerosis (ms) patients and normal subjects were tested for cellular hypersensitivity, using the leukocyte migration inhibition test lmi), to a parainfluenza-1-virus (6/94), previously isolated from brain biopsy tissue of an ms patient. the response of ms patients as a group was not greater than that of normals to 6/94, but a significant increase in the response was observed among selected patients in the early active and chronic advanced stages of the disease. ms patients, however, did ...1975164416
[cellular immunity in subacute sclerosing panencephalitis and multiple sclerosis]. 1975164635
[examination of serological tests for viral diseases of the respiratory tracts in livestock. the participation of respiratory viruses in the infections and their present epizootological assessment]. 1975164716
[isolation and identification tests for the parainfluenza-3 and bovine rhinotracheitis (ibr) viruses. the comparative value of serum neutralization, hemadsorption inhibition and immunofluorescence reactions in the diagnosis of pi-3 and ibr viral infections of calves]. 1975164717
problems on the etiological importance of pasteurella, mycoplasma and parainfluenza-3-virus in enzootic pneumonia of calves. 1975164719
a contribution to the pathogenesis of respiratory infections of complex aetiology in calves. 1975164720
parainfluenza-3 virus in cattle: mechanisms of infections and defence in the respiratory tract. 1975164721
the effect of cyclophosphamide on the defense to infection in calves. 1975164724
[vaccines and vaccination using live and inactivated viruses against pulmonary diseases in cattle]. 1975164725
pneumonia caused in healthy calves by contact exposure to coughing calves. 1975164728
[serological variants of type 1 parainfluenza viruses].a comparative study of the antigenic properties of parainfluenza type 1 viruses revealed the existence of 2 serological variants among them. the expedience of using the reference and recent years of isolates of parainfluenza type 1 viruses for serodiagnosis was demonstrated. highly immunogenic strains isolated in 1971 were selected for properation of hyperimmune sera. it is necessary for identification of new isolates of parainfluenza type 1 viruses to use antisera for strains isolated in recent ...1975164737
circulating antibodies and respiratory virus infections.most respiratory viruses infect only the respiratory mucosa and mainly the surface epithelium, and it is therefore plausible that antibody within the circulation is unable to influence them, either by preventing infection or by terminating it once it has started. however there is experimental and observational evidence that some infections are prevented by circulating antibody. this may be because circulating immunoglobulins are released onto the surface possibly because of virus cytopathic effe ...1975165114
meaning of circulating antibody titers in the infections of parainfluenza 3 virus and rs is generally accepted that secretory antibodies play a very important role in the prevention of respiratory infections, but it is also considered that the circulating antibody level is a very significant indication of the establishment of infections. in parainfluenza 3 and rs virus infections, reinfections are very common but seem to depend more or less upon circulating antibody levels. there is a correlation between circulating antibody level and infection rate in parainfluenza 3 infection i ...1975165115
contribution to the study of the parainfluenza antigens.from epidemiological data it appeared that an epidemic of parainfluenza type 1 occurred in lyons in october-november 1967. thereafter a few sporadic cases were detected. then in 1970 strains were isolated which showed a change in the hemagglutinin antigen. the persistence of antibodies in the population is shorter than that of the other parainfluenza viruses (i. e. parainfluenza 3); it is not known if reinfections with parainfluenza 1 occur since there are cross-reactions by cf test between para ...1975165116
an animal model for studying infection and immunity to and attenuation of human parainfluenza viruses.neonatal ferrets were found to be highly via the intranasal route of inoculation to infection with wild type isolates of parainfluenza viruses. wild type infection consistently resulted in deaths of these animals in 48 to 72 hours. autopsy and histopathological findings in lung tissue were consistent with those of viral infection. virus could consistently be isolated from lung tissues. using wild green monkey kidney grown viruses and selected passage levels of egg adapted viruses, virus attenuat ...1975165121
etiological relationship between several respiratory viruses and acute respiratory diseases in 1973 in the gdr.the role of some important respiratory viruses (influenza a, b, parainfluenza, adeno and mycoplasma) in morbidity due to acute respiratory diseases during 1973 in the gdr is analysed on account of serological data (cfr) obtained with paired sera (a -5 percent of all cases comprising all age groups) and virus isolations. the serological surveillance provides an important base for evaluating the significance of respiratory agents for the development of the morbidity due to ard.1975165122
immune response of cattle to intranasally or parenterally administered parainfluenza type 3 virus vaccines.the principal significance of pi-3 virus in the bovine respiratory disease complex would seem to be its role in predisposing the respiratory tract to bacterial infection by (5) disrupting the integrity of the superficial mucosa and (3) producing a more favourable milieu for bacterial growth. attention has therefore been given to establishing local immunity to preclude infection of superficial cells of the mucosa. criteria used to evaluate the degree of such immunity are (5) presence of specific ...1975165124
local and systemic response after simultaneous intranasal inoculation of temperature-sensitive mutants of parainfluenza 3, ibr and bovine adenovirus 3.triple seronegative calves were exposed by the nasal route to three (ts) mutants of bovine respiratory viruses (pi3, ibr, adeno3). after a single exposure, they responded with significant levels of serum antibodies to the three viruses. nasal antibodies were demonstrated for pi3 and adenovirus antigens. the failure to demonstrate nasal antibodies to ibr may be due to lack of sensitivity of the procedure used. when reexposed six weeks later, calves had sharp increases in levels of serum antibodie ...1975165126
thymus-dependent sensitizing processes involved in the induction of cns disease in mice by parainfluenza type 1 virus. 1975165240
[characteristics of clinical course of para-influenza infection in the newborns and infants of the first 3 months of life]. 1975165455
comparison of three methods for sampling the bovine upper respiratory tract for viruses.a modified naso-pharyngeal swab with nylon bristles had certain advantages for sampling the bovine upper respiratory tract for viruses. the titre of parainfluenza virus type 3 recovered was greatest when this type of swab was used compared with two other methods of sampling.1975165573
virus antibody levels in serum specimens from patients with optic neuritis and from matched controls;.virus antibody levels in serum specimens taken in acute and convalescent phases from 77 patients with optic neuritits were tested by measles hamagglutination inhibition (hi), measles hemolysis inhibition (hli), rubella hi, parainfluenza-1 hi, epstein-barr immunofluorescence (if), and against 11 other viruses and mycoplasma pneumoniae with the complement fixation (cf) technique. the virus antibody levels were indicated to be usually very stable, and a fourfold change in virus antibody levels was ...1975165652
virus antibody levels in the cerebrospinal fluid from patients with optic neuritis.virus antibody levels were studied in the cerebrospinal fluid (csf) of 58 patients with optic neuritis and 58 control patients with no indication of multiple sclerosis (ms) or infectious disorders of the central nervous system (cns). the specimens were tested against three different structural components of measles virus with measles hemagglutination inhibition (hi), measles hemolysis inhibition (hli) and gel precipitation (gp) tests. measles antibodies occurred in 62 per cent of csf specimens f ...1975165653
the sudden infant death syndrome and epidemic viral disease.a study was done to investigate the relationship between the sudden infant death syndrome (sids) and epidemic respiratory viral disease among hospitalized children under 18 months of age. during the 42 month-period of this study, there were 778 sudden infant deaths in chicago and 3244 hospital admissions of children under 18 months for respiratory disease. four outbreaks of respiratory syncytial (rs) virus infections, three outbreaks of influenza a virus infections, and several small clusters of ...1975165718
serologic profile of children in a mexican highland community: prevalence of complement-fixing antibodies to mycoplasma pneumoniae, respiratory syncytial virus and parainfluenza viruses.the study investigated the prevalence of antibodies to five leading agents of childhood respiratory disease in the county of huixquilucan, mexico. tests of sera from a random sample of children between 3 months and 18 years of age confirmed serologically the presence of mycoplasma pneumoniae, respiratory syncytial (rs) virus and parainfluenza 1, 2 and 3 viruses in this relatively isolated highland community. highest overall antibody frequency of 64.2% was seen for parainfluenza 3, and antibody t ...1975165720
respiratory virus infections in children hospitalized in montreal, 1968 - 1972. 1975165872
[a contribution of the problems of participation of parainfluenza viruses type 1, 2 and 3 in the etiology of sporadic acute respiratory affections in preschool children and in the adult age (author's transl)]. 1975165905
cytotoxicity of alveolar macrophages for virus-infected cells. 1975166319
[research on substances with antiviral activity. ii. derivatives of n-substituted 2-chloro-3-formylindoles].thiosemicarbazones and amidinohydrazones of n-substituted 2-chloroindole-3-aldehydes were synthesized and investigated for antiviral activity against rna virus (parainfluenza type 3, ha-i/cr-8 stock) and dna virus (vaccinia virus, hid stock). the thiosemicarbazones of 1-(o,m,p,-chlorobenzoyl)-2-chloroindole-3-aldehydes were more active than methisazone against vaccinia virus.1975166871
virus isolation and titration at 33 degrees and 37 degrees c.various prototype viruses and original specimens were comparatively titrated in cell cultures at 33 degrees and 37 degrees c. higher titers at 37 degrees were consistently obtained with adenoviruses; for other viruses (enteroviruses, herpesvirus hominis, vaccinia virus, parainfluenza viruses) the titers were mostly identical at either temperature. original specimens and prototye strains showed the same behavior. the habit to cultivate viruses from throat swabs at 33 degrees c is unsatisfactory f ...1975167268
prevalence of ibr-bvd-pi3 infections. 1975167273
[viruses in the etiology of diseases of the upper respiratory tracts]. 1975167497
epidemiology of herpesvirus and respiratory virus infections. part2. clinical findings. 1975168128
a comparison of immunologic response to intranasal and intramuscular parainfluenza-3 live virus vaccines in beef calves challenged experimentally in the feedlot.feeder calves were vaccinated with modified live virus (mlv) bovine parainfluenza-3 (pi-3) vaccines by the nasal and parenteral routes. thirty days later they were challenged with the sf-4 strain of virus. serum hemagglutination-inhibition (hi) antibodies and neutralizing antibodies in nasal secretions were compared with unvaccinated control calves. both vaccines produced satisfactory circulating antibodies, but intranasal vaccination produced higher levels of antibodies in nasal secretions. aft ...1975168620
the nasal secretion and serum antibody response of lambs following vaccination and aerosol challenge with parainfluenza 3 virus.the nasal and serum neutralising antibody responses of lambs was compared following vaccination with live or inactivated parainfluenza 3 (pi 3) virus by either intramuscular (im) or intranasal (in) routes. nasal antibody was only detected following inoculation of live virus in or inactivated virus in freund's complete adjuvant (fca) im. immunity to aerosol challenge, as assessed by viral shedding from the nose, was conferred by (1) live virus administered by either route, (2) two im inoculations ...1975168628
the seattle virus watch. vi. observations of infections with and illness due to parainfluenza, mumps and respiratory syncytial viruses and mycoplasma pneumoniae.seattle virus watch families were observed, 1965-1969, for infections with paramyxoviruses and m. pneumoniae by agent isolation and antibody assay of serial sera. infection rates, based on serology, exceeded those in tecumseh where families contained fewer young children. rates per 100 person-years were 44.4 for parainfluenzavirus, 21.6 for respiratory syncytial (rs) virus and 12.3 for m. pneumoniae. preschool children experienced the highest rates for rs and parainfluenza-viruses but, for the l ...1975168766
clinical and immunologic responses of calves with colostrally acquired maternal antibody against parainfluenza-3 virus to homologous viral infection.exposure of colostrum-deprived calves and calves with colostrally acquired maternal antibody to aerosols of parainfluenza-3 (pi-3) virus resulted in signs of infection, leukopenia, and shedding of virus from the nasal passages. however, infection was not as severe in calves with colostrally acquired maternal antibody as it was in colostrum-deprived calves which did not have antibody to pi-3 virus before they were exposed. all calves responded immunologically to pi-3 virus, as indicated by resist ...1975168795
antibody levels to parainfluenza, rubella, measles, and influenza a virus in children with polymyositis.the cf and hi antibody titers to rubella and measles viruses, the cf titers to influenxa a, and the hi titers to parainfluenza 1, 2, and 3 were carried out on the sera of 20 patients with childhood polymyositis and their matched controls. the titers for measles, parainfluenxa 1, and influenza a were slightly higher for patients than for controls. the control group had antibody titers to rubella and parainfluenza 2 and 3 higher than or similar to those of patients. strong patterns or significant ...1975168906
in vitro and in vivo effect of 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide (ribavirin) on types 1 and 3 parainfulenza virus infections.1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide (ribavirin) had significant in vitro activity against type 1 parainfluenza (sendai) and type 3 parainogenic (ha-1) viruses. activity was manifested as inhibition of both viral cytopathogenic effect and of recoverable virus or viral hemagglutinin titer. the minimum sendai virus inhibitory concentration was determined to be approximately 3.2 mug/ml. previous studies had determined the minimum concentration inhibiting ha-1 virus was approximately ...1975169109
antibodies to parainfluenza 3 virus in cattle, sheep and goats from northern nigeria. 1975169621
effect of 2-deoxy-d-glucose and glucosamine on the growth and functions of respiratory syncytial and parainfluenza 3 viruses. 1975169626
[damaging effect of 2 simian viruses on mammalian cell chromosomes].a green monkey myxovirus (sv5) causes chromosome breaks in embryonal diploid fibroblasts of man, cotton rats, striped hairy-footed hamster, and in cells of one heteroploid line of newborn lamb kidney. adenovirus sv15 is mutagenic for all cultures under study (diploid strains of human and cotton rat embryo fibroblasts and three continuous kidney cell lines from newborn lambs). the mutagenic activity of these viruses may be manifested without their undergoing a complete cycle of replication.1975169630
serologic studies of acute respiratory infections in military personnel.the advantages, disadvantages, and uses of serological epidemiology are discussed in relation to acute respiratory infections in military personnel. the prevalence of antibody reflects both current and past experience with respiratory agents and is a measure of susceptinility. incidence data calculated by testing two serial serum samples, on entry and discharge from the service, has indicated high influenza and mycoplasma pneumoniae rates in south american recruits and low rates of adenovirus ...1975169640
[mitosis and viral infection in cell cultures. i. the effect of cytolytic viruses on the mitotic regime of monolayer cell cultures]. 1975169645
[virological studies on insemination bulls in hessia. 2; studies on antibodies against bvd and pi-3 virus]. 1975169805
viral susceptibility of a cell line derived from the pig oviduct.seventeen of 24 rna viruses and eight of nine dna viruses replicated in a cell line derived from a pig fallopian tube. the following rna viruses grew poorly in it: the virus of transmissible gastroenteritis of pig and the swine-influenza, sendai and bovine para-influenza type 3 viruses. among other rna viruses an untyped swine para-myxovirus and some picornaviruses, rhabdoviruses and togaviruses attained high titers and produced an extensive cytopathic effect. among the dna viruses a porcine ade ...1975169971
methods for the detection of viruses in bovine evaluation of selected commonly used procedures for the recovery of endogenous viral contaminants in bovine serum was undertaken. low speen centrifugation (25,000 x g) was found to be efficient for the recovery of bovine herpesvirus type 1 (bhv-1) and parainfluenza virus type 3(pi-3) in bovine serum. decreased infectivity titers were obtained when parainfluenza virus type 3, and to a lesser extent bovine herpes virus type 1, were concentrated using high speed centrifugation (100,000 x g) for ...1975170306
physicochemical and serological characteristics of respiratory virus fluorescein-isothiocyanate conjugates for fluorescent-antibody diagnosis.fluorescein-isothiocyanate (fitc) conjugates were prepared by improved methods from standard reference antisera to influenza a and b, mumps, parainfluenza 1, 2, 3, and 4, herpesvirus, respiratory syncytial virus, and adenovirus hexon. the antisera, prepared in a variety of animals, were fractionated three times with selected optimal concentrations of ammonium sulfate and yielded gamma globulins of adequate purity for conjugation with fitc. conjugates containing optimal fluorescein-to-protein rat ...1975170315
measurement of parainfluenza-3 virus antibody by the single radial hemolysis technique.a single radial hemolysis assay for parainfluenza-3 virus antibody is described as a simple, sensitive, accurate, and precise alternative to hemagglutination inhibition and serum neutralization tests. a highly significant correlation exists between single radial hemolysis zone areas and hemagglutination inhibition titers of both serum and nasal secretion samples. antibody conversions equivalent to as little as a twofold rise in hemagglutination inhibition titer are readily and reliably detected ...1975170317
serological survey of parainfluenza and parotitis in the population of czechoslovakia.a serological survey was carried out to determine the prevalence and age distribution of hi antibodies against m. parainfluenzae type 1, 2, 3 and 4a and against m. parotitis in the population on from 7 regions of the czech socialist republic. while the distribution of antibodies to m. parainfluenzae is similar in all regions under study, considerable regional differences have been found in the occurence of antibodies against parotitis virus.1975170333
a review of routine tests for respiratory viruses in hospital inpatients.during the period june 1967 to may 1972, viral tests were performed upon 1195 inpatients aged 12 and over in the brompton hospital. the overall diagnostic rate was 21-5%, comparing 9-3% by isolation and 14-9% by complement fixation (6-5% by four-fold rises in titre in paired sera, 8-4% by titres larger than or equal to 160 in single sera). only 42% of all patients had an acute respiratory illness within one week prior to admission, which seriously curtailed the chances of isolating viruses and o ...1975170701
the response of normal and iron anemic calves to nasal infection with an attenuated strain of parainfluenza-3 virus. 1975170811
immunoglobulins and their relative neutralizing efficiency in cattle immunized with infectious bovine rhinotracheitis--parainfluenza-3 (ibr-pi-3) virus vaccine.the localization of anti-ibr and anti-pi-3 activity in the serum and nasal secretory immunoglobulins following intranasal immunization of cattle with a mixed vaccine (ibr-pi-3, mlv, tco) was studied and was found to reside in the nasal secretory iga, serum igm and igg fractions. the computation of their relative virus neutralizing efficiencies from kinetic data revealed their order of neutralizing efficiencies to be igm greater than iga greather than igg.1975170885
[muscle cell structure in polymyositis].electron microscopic investigations of muscles from patients with chronic polymyositis demonstrated presence of cytoplasmatic, tubular structures and extracellular virus-like particles. their size, morphology and location suggest multiplication of viruses from the group of parainfluenza viruses within the muscle cell in "infected" cultures of human fetal muscles. beside viruses belonging to the parainfluenza group presence of viruses from the herpes group was demonstrated. the latter belong to l ...1975171596
immune response and the b blood group locus in chickens.chickens of three blood group genotypes, b1b1, b1b2 and b1b19, were compared in their ability to produce antibodies. the b locus is the major histocompatibility locus in this species. homozygous b1 pullets had significantly higher adult mortality than did the b1 heterozygotes. in two separate experiments the b1b1 females produced significantly fewer antibodies to salmonella pullorum than did the b1 heterozygotes. also the b1b1 pullets responded with lower antibody titers following immunization w ...1975172405
virologic studies in rheumatoid arthritis.comparison of virus antibody titers in jra and matched controls failed to implicate rubella, measles, parainfluenza type 1 or epstein-barr viruses. neither rna viruses or mycoplasma were detected in rheumatoid synovial cell cultures by 3h-uridine incorporation. mycoplasma were not isolated from rheumatoid synovial membranes or cell cultures using the large specimen-broth culture procedure. the balance of available evidence, including our own, suggests that mycoplasma are not involved in ra. viru ...1975173010
[clinico-morphological comparisons in para-influenza]. 1975173026
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