| mapping of fmr1, the gene implicated in fragile x-linked mental retardation, on the mouse x chromosome. | a genetic map of the cf-9 to dmd region of the mouse x chromosome has been established by typing 100 offspring from a mus musculus x mus spretus interspecific backcross for the four loci cf-9, cdr, gabra3, and dmd. the following order and genetic distances in centimorgans were determined: (cf-9)-2.4 +/- 1.7-(cdr)-2.0 +/- 1.4-(gabra3)-4.1 +/- 2.0-(dmd). six backcross offspring carrying x chromosomes with recombination events in the cdr-dmd region were identified. these recombination events were u ... | 1992 | 1572655 |
| localization of the properdin factor complement locus pfc to band a3 on the mouse x chromosome. | the locus for properdin (properdin factor complement, pfc), a plasma glycoprotein, has been mapped to band a3 of the mouse x chromosome by in situ hybridization to metaphase spreads containing an x;2 robertsonian translocation. the x-linkage of the locus has also been confirmed by analysis of mus musculus x mus spretus interspecific crosses. the xa3 localization for pfc places it in the chromosomal segment conserved between man and mouse which is known to contain at least six other homologous lo ... | 1990 | 2272504 |
| close genetic and physical linkage between the murine haemopoietic growth factor genes gm-csf and multi-csf (il3). | the two murine haemopoietic growth factors, granulocyte-macrophage colony stimulating factor (gm-csf) and multi-csf (interleukin 3) stimulate the proliferation and differentiation of an overlapping set of haemopoietic progenitor cells and are produced coordinately following activation of t lymphocytes. here we report the chromosomal location of the genes encoding these two factors. initially both genes were assigned to chromosome 11 by analysis of mouse/chinese hamster somatic cell hybrids. gene ... | 1987 | 3034600 |
| two-dimensional electrophoresis of proteins: an updated protocol and implications for a functional analysis of the genome. | the two-dimensional electrophoresis (2-de) technique developed by klose in 1975 (humangenetik 1975, 26, 211-234), independently of the technique developed by o'farrell (j. biol. chem. 1975, 250, 4007-4021), has been revised in our laboratory and an updated protocol is presented. this protocol is the result of our experience in using this method since its introduction. many modifications and suggestions found in the literature were also tested and then integrated into our original method if advan ... | 1995 | 7498127 |
| genes encoding the h,k-atpase alpha and na,k-atpase alpha 3 subunits are linked on mouse chromosome 7 and human chromosome 19. | we have used linkage analysis and fluorescence in situ hybridization to determine the chromosomal organization and location of the mouse (atp4a) and human (atp4a) genes encoding the h,k-atpase alpha subunit. linkage analysis in recombinant inbred (bxd) strains of mice localized atp4a to mouse chromosome (chr) 7. segregation of restriction fragment length polymorphisms in backcross progeny of mus musculus x mus spretus mating confirmed this assignment and indicates that atp4a and atp1a3 (gene enc ... | 1993 | 7904196 |
| effects of provirus integration in the tpl-1/ets-1 locus in moloney murine leukemia virus-induced rat t-cell lymphomas: levels of expression, polyadenylation, transcriptional initiation, and differential splicing of the ets-1 mrna. | the tpl-1 locus was defined as a genomic dna region which is targeted by provirus insertion during progression of moloney murine leukemia virus-induced rat t-cell lymphomas. using a panel of 156 (mus musculus x mus spretus) x mus musculus interspecific backcross mice, we mapped tpl-1 to mouse chromosome 9 at a distance of 1.2 +/- 0.9 centimorgans from the ets-1 proto-oncogene (s.e. bear, a. bellacosa, p.a. lazo, n.a. jenkins, n.g. copeland, c. hanson, g. levan, and p.n. tsichlis, proc. natl. aca ... | 1994 | 8139017 |
| oocytes from pachytene to dictyotene can easily be analysed in neonatal rodents. | we have investigated the dynamics of meiotic prophase i in neonatal ovaries from different wild rodent species, from a laboratory strain of mus musculus and from mus musculus x mus spretus f1 hybrids. we found that almost all stages of prophase i were regularly present in neonatal ovaries from these species and that their transcriptional activity can be assessed by [3h]-uridine incorporation, indicating that postnatal analysis of meiotic chromosomes and synapsis may be conducted as an alternativ ... | 1993 | 8156159 |
| new insights into the man-mouse comparative map of the x chromosome. | two conserved loci, dxhx674h and dxhx679h, which map to xp11.22-xp11.21 on the human x chromosome short arm, have been positioned between the loci for proteolipid protein (plp) and the e1a subunit of pyruvate dehydrogenase (pdha1) in the distal region of the mouse x chromosome using mus musculus x mus spretus interspecific backcrosses. these data, together with previous comparative mapping studies on another conserved locus (dxf34) and the locus that encodes the erythroid transcription factor (g ... | 1994 | 8188251 |
| partial inversion of gene order within a homologous segment on the x chromosome. | the locus for the erthyroid transcription factor, gata1, has been positioned in the small interval between dxs255 and timp on the proximal short arm of the human x chromosome (chr) by use of a partial human cdna clone and a well-characterized somatic cell hybrid panel. analysis of selected recombinants from 108 mus musculus x mus spretus backcross progeny with the same clone confirmed that the homologous murine locus (gf-1) lies between otc and the centromere of the mouse x chr. these data imply ... | 1993 | 8431636 |
| high-resolution comparative mapping of the proximal region of the mouse x chromosome. | the murine homologues of the loci for mcleod syndrome (xk), dent's disease (cicn5), and synaptophysin (syp) have been mapped to the proximal region of the mouse x chromosome and positioned with respect to other conserved loci in this region using a total of 948 progeny from two separate mus musculus x mus spretus backcrosses. in the mouse, the order of loci and evolutionary breakpoints (eb) has been established as centromere-(dxwas70, dxhxf34h)-eb-clcn5-(syp, dxmit55, dxmit26)-tfe3-gata1-eb-xk-c ... | 1995 | 8530041 |
| the rapidly evolving pem homeobox gene and agtr2, ant2, and lamp2 are closely linked in the proximal region of the mouse x chromosome. | the pem gene encodes a homeodomain-containing protein expressed in reproductive tissue that may function as a transcription factor regulating spermatogenesis and sperm maturation. we have mapped the pem gene to the proximal end of the mouse x chromosome, placing it within the hprt region. based on the mapping of pem and other loci in three separate mus musculus x mus spretus backcross panels, we established the order of markers within this segment of the hprt region as: agtr2-pem-ant2-dxmit50-la ... | 1997 | 9344676 |
| an integrated genetic and man-mouse comparative map of the dxhxs674-pdha1 region of the mouse x chromosome. | the genes for ocular albinisim type 1 (oa1) and the xenopus laevis-like apical protein (apxl) map between amelogenin (amelx) and the pseudoautosomal boundary in the distal region of the human x chromosome short arm. the mouse homologues, oa1 and apxl, have recently been shown to lie proximal to their expected locations on the mouse x chromosome, but their positions with respect to critical gene loci in the vicinity have not been defined. by analyzing recombination events from (mus musculus x mus ... | 1998 | 9503026 |
| identification of stk6/stk15 as a candidate low-penetrance tumor-susceptibility gene in mouse and human. | linkage analysis and haplotype mapping in interspecific mouse crosses (mus musculus x mus spretus) identified the gene encoding aurora2 (stk6 in mouse and stk15 in human) as a candidate skin tumor susceptibility gene. the stk6 allele inherited from the susceptible m. musculus parent was overexpressed in normal cells and preferentially amplified in tumor cells from f(1) hybrid mice. we identified a common genetic variant in stk15 (resulting in the amino acid substitution f31i) that is preferentia ... | 2003 | 12881723 |
| the histone code regulating expression of the imprinted mouse igf2r gene. | the mouse igf-ii receptor (igf2r) and its antisense transcript air are reciprocally imprinted in most normal tissues. several mechanisms have been hypothesized to explain igf2r-air imprinting, including igf2r silencing by air, and transcriptional repression of igf2r-air by two differentially methylated regions (dmr1 and dmr2). we employed mus musculus x mus spretus interspecific mice and chromatin immunoprecipitation (chip) to investigate allele-specific histone modifications in the two dmrs. we ... | 2003 | 12975326 |
| global survey of escape from x inactivation by rna-sequencing in mouse. | x inactivation equalizes the dosage of gene expression between the sexes, but some genes escape silencing and are thus expressed from both alleles in females. to survey x inactivation and escape in mouse, we performed rna sequencing in mus musculus x mus spretus cells with complete skewing of x inactivation, relying on expression of single nucleotide polymorphisms to discriminate allelic origin. thirteen of 393 (3.3%) mouse genes had significant expression from the inactive x, including eight no ... | 2010 | 20363980 |