Publications

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ecology of phlebotomine sand flies (diptera: psychodidae) in a focus of leishmania (viannia) braziliensis in northeastern colombia.the phlebotomine sand fly fauna of two coffee plantations in a leishmania-endemic area of norte de santander, colombia was studied. regular insect collections using a variety of methods were made for three and a half years. information was obtained on diurnal resting sites, host range and seasonal abundance for 17 species, of which five (lutzomyia spinicrassa, lu. serrana, lu. shannoni, lu. ovallesi and lu. gomezi) were far more numerous than the others, anthropophilic and present throughout the ...19921343648
cutaneous leishmaniasis in tachira state, venezuela.cutaneous and mucocutaneous leishmaniases are widely spread in the mountainous andean regions of south america. in venezuela, these regions consist of the coffee-growing states of trujillo, merida and tachira. entomological and parasitological investigations in three geographically different climatic zones (lomas bajas, delicias and la grita) in tachira state have shown a predominance of the sandfly species lutzomyia spinicrassa (verrucarum group) and two leishmania species, leishmania mexicana ...19948192512
characterization of cell cultures derived from lutzomyia spinicrassa (diptera: psychodidae) and their susceptibility to infection with leishmania (viannia) braziliensis.the sand fly lutzomyia spinicrassa (morales, osorno-mesa, osorno & de hoyos, 1969) is a vector of leishmania (viannia) braziliensis, an etiological agent of cutaneous leishmaniasis in colombia. the present article describes, for the first time, the morphological, karyotypical, and isozymatic characteristics of cell cultures derived from l. spinicrassa embryonic tissues as well as the interaction of l. braziliensis with these cell cultures.200516319783
isolation of leishmania (viannia) braziliensis from lutzomyia spinicrassa (species group verrucarum) morales osorno mesa, osorno and hoyos 1969, in the venezuelan andean region.natural infection with leishmania spp. in phlebotomine sandflies was searched for during a longitudinal study carried out from july 1997 to july 1998 in the village catarnica, municipality independencia, táchira state. this hamlet is an old endemic focus of cutaneous leishmaniasis in the venezuelan andean region, which lies close to the colombian border at 1,300 m a.s.l., in an agricultural area mainly used for cultivating coffee. phlebotomine sandflies were collected using shannon traps placed ...200616605063
peritrophic matrix of phlebotomus duboscqi and its kinetics during leishmania major development.light microscopy of native preparations, histology, and electron microscopy have revealed that phlebotomus duboscqi belongs to a class of sand fly species with prompt development of the peritrophic matrix (pm). secretion of electron-lucent fibrils, presumably chitin, starts immediately after the ingestion of a blood meal and, about 6 h later, is followed by secretion of amorphous electron-dense components, presumably proteins and glycoproteins. the pm matures in less than 12 h and consists of a ...200919471970
identification of six new world leishmania species through the implementation of a high-resolution melting (hrm) genotyping assay.leishmaniases are tropical zoonotic diseases, caused by parasites from the genus leishmania. new world (nw) species are related to sylvatic cycles although urbanization processes have been reported in some south american countries such as colombia. this eco-epidemiological complexity imposes a challenge to the detection of circulating parasite species, not only related to human cases but also infecting vectors and reservoirs. currently, no harmonized methods have been deployed to discriminate th ...201425394418
life tables and reproductive parameters of lutzomyia spinicrassa (diptera: psychodidae) under laboratory conditions.lutzomyia spinicrassa is a vector of leishmania braziliensis in colombia. this sand fly has a broad geographical distribution in colombia and venezuela and it is found mainly in coffee plantations. baseline biological growth data of l. spinicrassa were obtained under experimental laboratory conditions. the development time from egg to adult ranged from 59 to 121 days, with 12.74 weeks in average. based on cohorts of 100 females, horizontal life table was constructed. the following predictive par ...200415558171
[new findings on the geographic distribution of the verrucarum group (diptera: psychodidae) in colombia].the incrimination of sand flies belonging to verrucarum species group in the leishmaniasis transmission underscores the need for a detailed information on the geographical distribution of these species. the current listing adds 34 new records that extend significantly the knowledge of the geographical distribution of the verrucarum group in colombia. the most important new records pertain to lutzomyia spinicrassa in the tropical dry forest of the atlantic coast, lutzomyia ovallesi in the orinoco ...200314582337
[karyotypic comparison of five species of lutzomyia (diptera: psychodidae) of the series townsendi and the verrucarum group in colombia].cytogenetic characteristics were established for five native species of phlebotomine sand flies (lutzomyia, series townsendi, verrucarum group): lutzomyia longiflocosa, lutzomyia townsendi, lutzomyia spinicrassa, lutzomyia torvida and lutzomyia youngi. karyotypes and chromosomal morphometry were compared. using the squash technique, brain tissues from late 4th instar larvae provided the necessary mitotic chromosomes. chromosomal measurements were made on the following chromosomal characteristics ...200212596447
peritrophic envelopes of lutzomyia spinicrassa (diptera: psychodidae).the secretion, morphology, and chemical composition of the peritrophic envelope were studied in the phlebotomine sand fly, lutzomyia spinicrassa morales, osornomesa, osorno & hoyos, a suspected vector of leishmania braziliensis in colombia and venezuela. viewed under light microscopy, the envelope matured rapidly and could be dissected from the blood bolus as early as 12 h and until 36 h after feeding; subsequently it began to degrade. the envelope was initially a closed sac around the blood mea ...19957473627
vectors of leishmaniases in venezuela.information on the vectors of leishmaniases in venezuela is reviewed and updated. lutzomyia longipalpis is considered the vector of visceral leishmaniasis. lutzomyia ovallesi of the northcentral area is the only human feeding species from which parasites have been isolated and typed as leishmania braziliensis. in the andean region there is strong evidence that lu. youngi is the vector of cutaneous leishmaniasis but the parasites require further identification. lutzomyia spinicrassa may be respon ...19911841212
establishment and characterization of an embryonic cell line from sarconesiopsis magellanica.sarconesiopsis magellanica (le guillou) (diptera: calliphoridae) is a necrophagous fly that is important in both human and veterinary medicines. this insect has been registered in colombia as a biological indicator in estimating post-mortem interval. insect cell cultures are an important biotechnological tool for basic and applied studies, and cell cultures derived from s. magellanica embryonic tissues are described in this study. s. magellanica embryonated eggs were taken for tissue explants. t ...024766352
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