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isolation and partial characterisation of a new virus causing acute haemorrhagic fever in zaire. 197765661
viral haemorrhagic fever in southern sudan and northern zaire. preliminary studies on the aetiological agent. 197765662
isolation of marburg-like virus from a case of haemorrhagic fever in zaire. 197765663
after marburg, ebola. 197765668
decreasing any viral infectivity in blood-smears for malarial parasite examination. 197874655
the nature and classification of viruses of man. 1979111266
ebola virus and hemorrhagic fever: andromeda strain or localized pathogen? 1979111590
[viral contamination in laboratories and hospital units].viral contamination is at least as important in hospital laboratories and wards as contamination by bacteria or microscopic fungi, but it is much more insidious and sometimes unrecognized. there are two main types: the first has a purely technical effect and only interest the virologist. this is contamination of reagents, reference strains, cell cultures, etc.., by foreign viral agents. it may be the cause of errors on diagnosis or regrettable errors of interpretation of certain experimental dat ...1975165448
biohazards and simian viruses.nonhuman primates are extensively used in laboratories as experimental animals. it is necessary, however, to realize that their employment may be dangerous to man and other species of primates, if recognition of their flora and fauna, especially viral, is not considered and if appropriate controls are not followed. several outbreaks have occurred, resulting in high mortality and morbidity of man and simian. a number of recommendations are provided which, if followed, will minimize the waste of t ...1975169837
marburg virus.marburg virus disease, which produced 20 per cent mortality when it first occured during 1967 in germany and yugoslavia, recently appeared again in south africa. the source of the first outbreak was monkeys shipped from africa; the origin of the second episode is unclear. because distribution of the virus in nature is unknown, its threat to man cannot be readily determined. differential laboratory diagnoses of hemorrhagic fevers should be encouraged in order to learn more about the epidemiology ...1976829039
[the effect of the methods for producing an experimental marburg virus infection on the characteristics of the course of the disease in green monkeys].a comparative study on the features of the pathogenesis of marburg disease after parenteral and aerosol infection of green monkeys with a virus prepared from native culture suspension and that after lyophilization was carried out. the changes in the dynamics of the clotting time, the activity of serum aminotransferases, the duration of prefebrile period and survival time were analysed in different cases. no lethality was observed in animals infected with small doses of aerosol preparations.19921441442
[a comparative study of the in-vitro interaction of the marburg virus with macrophages from different animal species].the amplification of marburg virus in primary cultures of peritoneal macrophages of animals with different sensitivity to infection with this virus, as well as the capacity of this virus to adsorb on macrophages were studied. macrophages of the animals sensitive to marburg virus were capable to support its reproduction in vitro whereas those of resistant animals were not. macrophages of the animals with intermediate sensitivity were shown to be either completely resistant to virus reproduction o ...19911785187
[an experimental study of the contact transmission of the marburg virus].experiments in guinea pigs and m. rhesus monkeys showed the possibility of contact infection of animals with marburg virus. secondary infection occurred most intensively when the monkeys were kept together but was also shown to be possible when the animals were separated but placed in the direction of the air flow from the sick monkeys as well as by "nose-to-nose" contact excluding the alimentary mode of transmission and the role of the agent excreted in the urine.19911785188
[a comparative study of the immunological indices in guinea pigs administered an inactivated marburg virus].samples of concentrated and unconcentrated preparations of marburg virus were studied. the ultrafiltration method helped increase the specific protein content and decrease the common protein content in a preparation. the resistance index increased with an increase of the specific protein content and the maximum (2.63 +/- 0.2) was reached by two immunizations on 0 and 14 days.19911803779
[the sensitivity of different experimental animals to the marburg virus]. 19911803784
[the sensitivity of different cell culture lines to the marburg virus]. 19911803785
[a comparison of the methods of fluorescent antibodies and solid-phase immunoenzyme analysis in the detection of marburg virus antibodies]. 19911796591
[the detection of the marburg virus antigen by solid-phase immunoenzyme analysis].comparative studies of two variants of the enzyme-linked immunosorbent assay (elisa) were carried out to determine the sensitivity of the detection of marburg virus antigens in vero cells. both competitive and two-antibody elisa variants detected as little as 5 ng of marburg virus antigen. the vero cell monolayer was found to produce 5-50 ng/0.05 ml of the virus-specific proteins at 6 to 8 days postinfection.19911725078
marburg virus, a filovirus: messenger rnas, gene order, and regulatory elements of the replication cycle.the genome of marburg virus (mbg), a filovirus, is 19.1 kb in length and thus the largest one found with negative-strand rna viruses. the gene order - 3' untranslated region-np-vp35-vp40-gp-vp30-vp24-l-5' untranslated region-resembles that of other non-segmented negative-strand (nns) rna viruses. six species of polyadenylated subgenomic rnas, isolated from mbg-infected cells, are complementary to the negative-strand rna genome. they can be translated in vitro into the known structural proteins n ...19921626422
detection of ebola-like viruses by immunofluorescence. 19901979412
the nucleotide sequence of the l gene of marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses.the nucleotide sequence of the l gene of marburg virus, strain musoke, has been determined. the l gene has a single long open reading frame encoding a polypeptide of 2330 amino acids (mw 267,175) that represents the viral rna-dependent rna polymerase. the putative transcription start signal (3'cuaccuauaauu 5') and the termination signal (3' uaauucuuuuu 5') of the gene could be identified. computer-assisted comparison of the l protein with l proteins of other nonsegmented negative-stranded rna vi ...19921546452
antibody prevalence against haemorrhagic fever viruses in randomized representative central african populations.between 1985 and 1987, 5,070 randomly selected persons living in 6 central african countries (cameroon, central african republic, chad, congo, equatorial guinea and gabon) were checked for serological evidence of haemorrhagic fever. rural and urban areas were studied, including ecoclimatic zones ranging from dry savana to tropical rain forest. virus-reactive antibodies were found with all antigens tested, and the global prevalence of positive sera was distributed as follows: crimean-congo haemor ...19892505350
sequence analysis of the marburg virus nucleoprotein gene: comparison to ebola virus and other non-segmented negative-strand rna viruses.the first 3000 nucleotides from the 3' end of the marburg virus (mbg) genome were determined from cdna clones produced from genomic rna and mrna. identified in the sequence was a short putative leader sequence at the extreme 3' end, followed by the complete nucleoprotein (np) gene. the 5' end of the np mrna was determined as was the polyadenylation site for the np gene. the transcriptional start (3' uucuucuuauaauu..) and termination (3' ..uaauucuuuuu) signals of the mbg np gene are very similar ...19921538192
carbohydrate structure of marburg virus glycoprotein.marburg virus was propagated in e6 cells, a cloned cell line of vero cells, in the presence of [6-3h]glucosamine. radiolabelled viral glycoprotein was digested with trypsin, and oligosaccharides were liberated by sequential treatment with endo-beta-n-acetylglucosaminidase h, peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f and o-glycosidase, by beta-elimination, and by alkaline hydrolysis. after fractionation by hplc and gel filtration, glycans were characterized chromatographically, ...19921421752
[the immunogenic properties of marburg virus proteins].immunogenic and protective properties of vp40 and np proteins of marburg virus were studied. vp40 protein was shown to have insignificant immunogenicity and np protein to be capable of protecting the animals from lethal infection by stimulation of cell-mediated immunity. no significant increase in the specific antibody level was found.19921413715
outbreake of marburg virus disease in johannesburg.the first recognised outbreak of marburg virus disease in africa, and the first since the original epidemic in west germany and yugoslavia in 1967, occurred in south africa in february 1975. the primary case was in a young australian man , who was admitted to the johannesburg hospital after having toured rhodesia. two secondary cases occurred, one being in the first patient's travelling companion, and the other in a nurse. features of the illness included high fever, myalgia, vomiting and diarrh ...1975811315
ultrastructure of ebola virus particles in human liver.electron microscopy of tissues from two necropsies carried out in the sudan on patients with ebola virus infection identified virus particles in lung and spleen, but the main concentrations of ebola particles were seen in liver sections. viral precursor proteins and cores were found in functional liver cells, often aligned in membrane-bound aggregations. complete virions, usually found only extracellularly, were mainly seen as long tubular forms, some without cores. many tubular forms had 'enlar ...1978641193
[ecology of "marburgvirus" (rhabdovirus simiae)]. 19694989351
viral haemorrhagic fevers: properties and prospects for treatment and prevention. 19846091539
a consideration of the diagnosis of dangerous infectious fevers in south africa. 1978565951
modifications to indirect immunofluorescence tests on lassa, marburg, and ebola material. 19836131336
uveal involvement in marburg virus disease.the first reported case of uveal involvement in marburg virus disease is described.1977557985
[lassa fever and marburg disease]. 1978417467
[acute fever caused by marburg virus]. 1977413103
[the marburg virus group]. 1977406580
viral haemorrhagic fever antibodies in zimbabwe schoolchildren. 19826761909
[pathogenic microbiology of international communicable diseases]. 19806771443
[marburg virus disease: epidemiology, symptomatology, management and prognosis]. 19806771444
[marburg, lassa and ebola virus as cause of hemorrhagic fever]. 1978352653
early detection of antigen and estimation of virus yield in specimens from patients with marburg virus disease.autopsy specimens from patients with marburg disease having at least 10(4.5) tcid(50) of virus per gram of tissue were found to contain sufficient fluorescent antigen-positive cells to make a specific diagnosis possible in less than 3 h. liver, heart, spleen, and kidney tissues were found to contain significant amounts of virus. tissue suspensions, as well as blood or serum samples, inoculated into vero cell cultures produced virus-specific immunofluorescence within 2-5 days. at least one specim ...1978310364
international arbovirus research.international cooperation in arbovirus research became increasingly prominent shortly after it was found in the early 1940's, that antigenic relationships existed among certain viruses isolated in different areas of thw world. the interest of a number of scientists and of a private organization led to the establishment of an international information exchange and to the preparation of an international catalogue of arboviruses including certain other viruses of vertebrates. international cooperat ...1975173936
use of betapropionolactone inactivated ebola, marburg and lassa intracellular antigens in immunofluorescent antibody assay. 19827049096
[new high-risk infectious diseases]. 1978566498
ebola and marburg viruses: ii. thier development within vero cells and the extra-cellular formation of branched and torus forms.the development of marburg virus and the sudanese and zaire strains of ebola virus in vero cells as visualized by electron microscopy is described. despite differences in timing, all three strains appear to pass through identical stages of development. initially there is a large increase in nucleolus material, and viral precursor material arranges itself in spirals and then into tubes. the cells fill with core material, which passes to the plasmalemma, which often proliferates. each virion passe ...1979119829
ebola and marburg viruses: i. some ultrastructural differences between strains when grown in vero cells.a strain of marburg virus and two strains of ebola virus grown in vero cells were compared by electron microscopy. the outer coat of the marburg virion appeared to be more resistant to erosion by negative staining techniques than that of the epbola strains. marburg virus commonly produced "torus" forms and short filaments; the zaire strain of ebola produced extensive branched forms and very long filaments; the sudan strain of ebola produced shorter, less branched structures but very many aberran ...197994087
[certain pathogenetic characteristics of a disease in monkeys in infected with the marburg virus by an airborne route].time course of marburg virus (strain popp) accumulation and changes in hematological parameters were studied in aerosol infected m.rhesus monkeys. the lungs were the first organ in which the virus was detected after respiratory infection of monkeys. four days after inoculation the virus was detected in the liver, spleen, blood, and thymus. six days after inoculation the virus was present in virtually all organs and secretions. the period of fever was associated with manifest leukopenia in primat ...19957483565
[a promising method for preparative production and purification of the marburg virus].a method for the production of marburg virus in preparative amounts has been developed. it is based on sedimentation of the virus from blood plasma of infected guinea pigs by ultracentrifugation followed by purification in sucrose density gradient or gel chromatography on macroporous glass sorbents. the optimal terms of blood collection in infected animals were determined. purified virus did not lose its biological activity. concentrated virus preparations were studied by electron microscopy and ...19957483566
[marburg virus: the first determined nucleotide sequence of two genes].the preparations of purified marburg virus were isolated from blood plasma of infected guinea pigs and characterized. viral rna was extracted from the virions. the cdna was synthesized on the isolated rna matrix by the reverse transcriptase with the use of dissipated priming. the obtained cdna was inserted into the plasmid pbr322 by the connector technique and the resulting recombinant plasmids were cloned in escherichia coli cells. the specific clones selected by molecular hybridization method ...19911857371
[marburg hemorrhagic fever]. 19911882631
[viral haemorrhagic fever].two outbreaks of haemorrhagic fever caused by a marburg-like virus occurred in the sudan and zaire from august to november 1976. the inital epidemiological investigations and emergency control measures were carried out by sudanese and zairian health personnel and several of them unfortunately died in the course of their duties. in view of the unusual gravity of the outbreaks and the unknown nature of the agent, assistance was provided by a who team in the sudan and a multinational team in zaire. ...1977590991
a single amino acid change in the e2 glycoprotein of venezuelan equine encephalitis virus affects replication and dissemination in aedes aegypti mosquitoes.four monoclonal antibody-resistant variants (marvs) of venezuelan equine encephalitis (vee) virus were used to study mosquito-virus interactions. in vitro experiments using an aedes albopictus cell line, c6/36, demonstrated that an amino acid change in the glycoprotein e2h epitope (marv 1a3b-7) decreased virus growth when compared with the wild-type, trinidad donkey virus, and its vaccine derivative, tc-83. the marvs replicated as efficiently as the parent virus when inoculated into aedes aegypt ...19911919525
[infections by marburg and ebola viruses: guide for their diagnosis, treatment and control]. 1978150845
antibodies to haemorrhagic fever viruses in madagascar populations.sera of 381 adult people from 5 areas in madagascar were tested by the indirect immunofluorescence method for antibodies against congo-crimean haemorrhagic fever and rift valley fever viruses (bunyaviridae), ebola (strains zaire and sudan) and marburg viruses (filoviridae), and lassa virus (arenaviridae). the highest prevalence rate was that of ebola virus (4.5%). as no haemorrhagic syndrome has been found associated with this virus, the possible presence of a less pathogenic, antigenically rela ...19892515626
hemorrhagic fever virus infections in an isolated rainforest area of central liberia. limitations of the indirect immunofluorescence slide test for antibody screening in africa.serum samples from 119 healthy individuals and 106 epilepsy patients inhabiting grand bassa county, liberia, were tested for antibodies to hemorrhagic fever viruses (hfv) by indirect immunofluorescence. e6 vero cells infected with lassa fever virus (las), rift valley fever virus (rvf), congo hemorrhagic fever virus (con), marburg virus (mbg) and the ebola (ebo) virus strains mayinga (may) and boniface (bon) were used as antigen. to obtain reproducible and specific test results sera had to be abs ...19863092415
viral hemorrhagic fever antibodies in nigerian populations.using the immunofluorescence test, a serosurvey for antibodies to five viral agents associated with hemorrhagic febrile infections was conducted with 1,677 human sera from different parts of nigeria. three hundred fifty-seven (21.3%) were positive for lassa virus antibody, while antibodies to rift valley fever virus were detected in 42 (2.5%) of the sera. testing for rift valley fever virus antibody was confirmed by plaque reduction neutralization test. antibodies to ebola and marburg viruses we ...19883128130
physicochemical properties of marburg virus: evidence for three distinct virus strains and their relationship to ebola virus.the physicochemical and antigenic properties of three groups of marburg (mbg) virus isolates, separated temporally and geographically, were compared to each other and to another member of the same family, ebola (ebo) virus. each mbg isolate contained seven virion proteins, one of which was a glycosylated surface protein. peptide mapping of glycoproteins, nucleoproteins (np) and viral structural protein (vp40) demonstrated extensive sequence conservation in the proteins of viruses isolated over a ...19883404120
[method for the simultaneous detection of immunofluorescing antibodies to the causative agents of different hemorrhagic fevers]. 19853907144
[research with the marburg, lassa and ebola viruses]. 19938059520
conservation of the 3' terminal nucleotide sequences of ebola and marburg virus.the 3' rna base sequences of several marburg (mbg) and ebola (ebo) virus isolates have been determined. a comparison of these 3' terminal noncoding sequences with those of other negative strand rna viruses suggests a unique phylogenic niche for marburg and ebola viruses. the translation initiation site and 35 n-terminal amino acids of the 3' proximal coding gene of a zaire strain of ebola virus was predicted. in addition, putative leader rna sequences preceding the first gene are discussed in te ...19863946083
[complement-fixing antibodies in man after infection with "marburgvirus" (rhabdovirus simiae)]. 19695393201
maridi haemorrhgic fever: a new viral disease (author's transl).a new viral disease (maridi haemorrhagic fever) occurred in the south sudan in 1976. it was obviously identical with an epidemic which occurred at the same time in zaire. the virus is morpologically closely similar to the marburg virus. during the maridi epemic 124 of 238 patients died (52%). characteristic symptoms were fever and headache (100%), diarrhoea (83%), retrosternal pain (82%), vomiting (68%), haemorrhages (62%), morbilliform or vesicular rash (52%). at post-mortem there were changes ...197721783
inactivating lassa and marburg/ebola viruses. 19826123862
lack of cross reactivity of rhabdovirus antibodies with marburg and ebola antigens in the indirect immunofluorescent antibody test. 19826179483
antibodies against haemorrhagic fever viruses in kenya populations.human sera from lodwar (77 sera), nzoia (841 sera), masinga (251 sera), laisamis (174 sera) and the malindi/kilifi area (556 sera) in kenya were tested by indirect immunofluorescence for antibodies against marburg, ebola (zaire and sudan strains), congo haemorrhagic fever, rift valley fever and lassa viruses. antibodies against ebola virus, particularly the zaire strain, were detected in all regions and were, over-all, more abundant than antibodies against the other antigens. ebola and marburg a ...19836419422
physicochemical inactivation of lassa, ebola, and marburg viruses and effect on clinical laboratory analyses.clinical specimens from patients infected with lassa, ebola, or marburg virus may present a serious biohazard to laboratory workers. we have examined the effects of heat, alteration of ph, and gamma radiation on these viruses in human blood and on the electrolytes, enzymes, and coagulation factors measured in laboratory tests that are important in the care of an infected patient. heating serum at 60 degrees c for 1 h reduced high titers of these viruses to noninfectious levels without altering t ...19846490832
[mechanisms of protective immune response in models of marburg fever in monkeys].the parameters of nonspecific immunity (interferon, tumor necrosis factor, natural killers), of spontaneous and mitogen-induced lymphocyte proliferation, of specific immunity (antibodies to marburg virus, antigen-stimulated proliferation) were found to change in the course of experimental marburg fever in immune and intact animals. the named factors were shown to influence the course of the disease; their role in the lethal outcome of the infection was demonstrated.19957676670
marburg and ebola virus antibodies in kenyan primates. 19816113374
glycosylation and oligomerization of the spike protein of marburg virus.the oligosaccharide side chains of the glycoprotein of marburg virus (mw 170,000) have been analyzed by determining their sensitivity to enzymatic degradation and their reactivity with lectins. it was found that they consist of n- and o-glycans. studies employing chemical cross-linking showed that the glycoprotein is present as a homotrimer in the viral envelope.19912024471
preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys.a method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to lassa, marburg, and ebola viruses. the technique uses mixtures of specifically infected vero cells placed on teflon-templated microscopy slides. it was found to be as sensitive as the use of monovalent antigens for detection and quantitation of antibodies to these highly hazardous human pathogens.19817031084
inactivation of lassa, marburg, and ebola viruses by gamma irradiation.because of the cumbersome conditions experienced in a maximum containment laboratory, methods for inactivating highly pathogenic viruses were investigated. the infectivity of lassa, marburg, and ebola viruses was inactivated without altering the immunological activity after radiation with co60 gamma rays. at 4 degrees c, lassa virus was the most difficult to inactivate with a rate of 5.3 x 10(-6) log 50% tissue culture infective dose per rad of co60 radiation, as compared with 6.8 x 10(-6) log 5 ...19827153317
haemorrhagic fever in gabon. i. incidence of lassa, ebola and marburg viruses in haut-ogooué.a serological enquiry aimed at determining the incidence of infection with lassa, ebola and marburg viruses was conducted on the human population of the region of haut-ogooué (gabon) and on primates. the results, obtained by the indirect immunofluorescence technique, showed that more than 6% of the human population had had contact with ebola virus but no antibodies against marburg or lassa viruses were found. most sera reacted to an ebola antigen from a zairian strain, but showed little or no re ...19827164137
inactivated marburg virus elicits a nonprotective immune response in rhesus monkeys.in the present work the kinetics of some indices of immunity (tumor necrosis factor (tnf), interferon (ifn), natural killer cells (nk), lymphocyte proliferation activity, virus-specific antibodies, cd4/cd8 ratio) in response to marburg virus infection in macaca mulatta were studied. the different kinetics of immunological parameters for animals which survived marburg virus infection and for animals which died after infection are shown. a comparison of the indices of ifn, tnf and spontaneous lymp ...19968717394
characterization of a new marburg virus isolated from a 1987 fatal case in kenya.in 1987, an isolated case of fatal marburg disease was recognized during routine clinical haemorrhagic fever virus surveillance conducted in kenya. this report describes the isolation and partial characterization of the new marburg virus (strain ravn) isolated from this case. the ravn isolate was indistinguishable from reference marburg virus strains by cross-neutralization testing. virus particles and aggregates of marburg nucleocapsid matrix in ravn-infected vero cells, were visualized by immu ...19968800792
[antibodies against the marburg virus among human populations in the southeastern central african republic].a serological survey was carried out in the south-east of the central african republic in 1979 to investigate the incidence of marburg virus in the human population. serum samples were tested using the indirect immunofluorescence method on infected and inactivated vero cells (green monkey kidney) on slides prepared by the center for disease control, atlanta, u.s.a. out of 499 samples, 7 were positive. two of them showed a high antibody titre of 1/64. both patients were retested 10 months later a ...19816786769
[clinical-virusological characteristics of disease in guinea pigs, infected by the marburg virus aerogenically].marburg virus (strain popp) was found to accumulate in various organs of guinea pigs after aerogenic infection. at the initial stage of the infection primary multiplication of the virus took place in the lungs. the presence of the virus in bronchopulmonary washings 2-3 days after infection, leukopenia and hyperthermia 4 days after infection are the earliest virological and clinical signs of disease in aerogenically infected guinea pigs.19957676673
virologic investigation of a case of suspected haemorrhagic fever.after travelling in subsaharan africa, an area known for sporadic cases of marburg virus infection, a young swedish man presented with a classical picture of severe viral haemorrhagic fever complicated by disseminated intravascular coagulation and septicaemia. serum samples examined by electron microscopy revealed particles of a size compatible with filovirions. indirect fluorescent antibody tests indicated transient seroconversion to marburg virus. in lymphocyte transformation assays of cells i ...19947709077
[marburg virus and mononuclear phagocytes: study of interactions].interactions of marburg virus and mononuclear phagocytes of guinea pigs were studied using a complex of virologic methods and electron microscopy. active reproduction of marburg virus in peritoneal macrophages both in vitro and in vivo was revealed. the study showed that mononuclear phagocytes were the first target cells in guinea pigs intraperitoneally infected with marburg virus which provided virus dissemination into interstitial tissue of viscera. analysis of the results indicates an importa ...19947716905
update: management of patients with suspected viral hemorrhagic fever--united states.in 1988, cdc published guidelines for managing patients with suspected viral hemorrhagic fever (vhf) (1). pending a comprehensive review of the 1988 guidelines, this notice provides interim recommendations that update the 1988 guidelines for healthcare settings in the united states. this update applies to four viruses that cause syndromes of vhf: lassa, marburg, ebola, and congo-crimean hemorrhagic fever viruses; although the risk and/or mode of nosocomial transmission differs for each of these ...19957783731
[effectiveness of virus-specific proteins in immunogenesis during experimental marburg fever].the protective properties of early virus-specific proteins (evsp) of marburg virus have been studied. immunoglobulins to evsp in combination with immunoglobulins to uv-inactivated marburg virus prevented lethal infection in marburg virus infected guinea pigs. more than 80% of guinea pigs immunized by mixture of evsp and uv-inactivated marburg virus survived after challenge with 25 ld50 of marburg virus. the explanation of this phenomenon and its further utilization are discussed.19968967067
marburg, ebola and rift valley fever virus antibodies in east african primates.sera from 464 primates held at four institutes in kenya were tested by indirect immunofluorescence for the presence of antibodies against marburg, ebola, congo haemorrhagic fever, rift valley fever and lassa viruses. four of 136 vervet monkeys were positive for marburg virus antibodies and three of 184 baboons had antibodies against ebola virus. one baboon was positive for marburg virus antibodies. two vervet monkeys, three baboons and one grivet monkey (of 56 tested) had antibodies against rift ...19826810518
complete nucleotide sequences of marburg virus genes 5 and 6 encoding vp30 and vp24 proteins.nucleotide sequences of the genes 5 and 6 of the marburg virus, popp strain, were determined. orfs encoding polypeptides vp30 (281 a.a., mw 32,640) and vp24 (253 a.a., mw 28,621) were found. the putative transcription start and stop signals for viral rna-dependent rna polymerase were revealed for both genes. overlapping of genes 5 and 6 was shown. the deduced amino acid sequences of vp30 and vp24 proteins displayed significant homology with the analogous proteins of another filovirus, the ebola ...19957773195
[the penetration of the marburg virus into eukaryotic cells].the early stages of infection of vero-e6 cell culture with marburg virus, a member of filovirus family, highly pathogenic for man, were studied. virus multiplication was completely or significantly inhibited by lysosomotropic agents (lta) of two types: weak base (ammonium chloride) and ionophore monensin. the level of the inhibiting effect was proportional to lta concentration and was maximal when the drug was introduced into the culture medium before virus inoculation. complete inhibition of ma ...19938059526
the asialoglycoprotein receptor is a potential liver-specific receptor for marburg virus.the liver is one of the main target organs of marburg virus (mbg), a filovirus causing severe haemorrhagic fever with a high fatality rate in humans and non-human primates. mbg grown in certain cells does not contain neuraminic acid, but has terminal galactose on its surface glycoprotein. this observation indicated that the asialoglycoprotein receptor (asgp-r) of hepatocytes may serve as a receptor for mbg in the liver. binding studies revealed that the attachment of mbg to asgp-r-expressing hep ...19957844558
[a case of a laboratory infection with marburg fever]. 19947941853
sequence analysis of the ebola virus genome: organization, genetic elements, and comparison with the genome of marburg virus.sequence analysis of the second through the sixth genes of the ebola virus (ebo) genome indicates that it is organized similarly to rhabdoviruses and paramyxoviruses and is virtually the same as marburg virus (mbg). in vitro translation experiments and predicted amino acid sequence comparisons showed that the order of the ebo genes is: 3'-np-vp35-vp40-gp-vp30-vp24-l. the transcriptional start and stop (polyadenylation) signals are conserved and all contain the sequence 3'-uaauu. three base inter ...19938237108
[the immunity indices of animals immunized with the inactivated marburg virus after infection with homologous virus].the data on changes in the parameters of specific and nonspecific immunity after challenge of the immunized animals with marburg virus are presented. mediators of the immune response: tumor necrosis factor, interferon, were shown to play different roles in the development of the disease and "protection" of animals. the survival rate of the immunized animals after the challenge with marburg virus was determined by the intensity and level of the immune response after challenge rather than by the l ...19948160440
the nucleoprotein of marburg virus is phosphorylated.the nucleoprotein (np) of marburg virus (mbg), a filovirus, is encoded by the gene closest to the 3' end of the non-segmented negative-strand rna genome. sequence comparison has indicated that np is the functional equivalent to the nucleoproteins of paramyxoviruses and rhabdoviruses. expression of recombinant np in two eukaryotic systems using vaccinia virus and baculovirus (vectors psc11 and pacymb1, respectively) and analysis of mbg-specific proteins have demonstrated that the np of mbg is pho ...19948151297
replication of marburg virus in human endothelial cells. a possible mechanism for the development of viral hemorrhagic disease.marburg and ebola virus, members of the family filoviridae, cause a severe hemorrhagic disease in humans and primates. the disease is characterized as a pantropic virus infection often resulting in a fulminating shock associated with hemorrhage, and death. all known histological and pathophysiological parameters of the disease are not sufficient to explain the devastating symptoms. previous studies suggested a nonspecific destruction of the endothelium as a possible mechanism. concerning the imp ...19938473483
antibody to ebola virus in guinea pigs: tandala, zaire.a case-control study was conducted to investigate the findings of antibody to ebola virus in the serum of a guinea pig from tandala, zaire. case households, defined by the possession of one or more guinea pigs, were compared to neighboring households without guinea pigs. seven (5.1%) of 138 samples of human sera and 36 (26%) of 138 samples of guinea pig sera had antibody to ebola virus. there was no clustering of seropositivity among humans or guinea pigs within households, nor was there any ass ...19826750007
filovirus-induced endothelial leakage triggered by infected monocytes/macrophages.the pathogenetic mechanisms underlying hemorrhagic fevers are not fully understood, but hemorrhage, activation of coagulation, and shock suggest vascular instability. here, we demonstrate that marburg virus (mbg), a filovirus causing a severe form of hemorrhagic fever in humans, replicates in human monocytes/macrophages, resulting in cytolytic infection and release of infectious virus particles. replication also led to intracellular budding and accumulation of viral particles in vacuoles, thus p ...19968642644
[survival of marburg virus infectivity on contaminated surfaces and in aerosols].marburg virus was shown to survive for up to 4-5 days on contaminated surfaces. in aerosol it was not stable, the specific rate of its inactivation being 0.05 min-1. this brought the authors to a conclusion that a relatively close contact is needed for virus transmission from man to man, although the possibility of aerosol transmission of the infection may be appreciably increased in case of the hemorrhagic syndrome with a high level of viremia.19968669144
[disinfecting action of chloramine b on marburg virus]. 19968669148
the complete nucleotide sequence of the popp (1967) strain of marburg virus: a comparison with the musoke (1980) strain.the nucleotide sequence of genomic rna of marburg virus strain popp was determined. strain popp was isolated in 1967 during the first filoviral outbreak. the virus was purified from blood of infected guinea pigs in which it had been maintained. the length of the determined sequence was 19112 nucleotides. amino acid sequences of seven known virion proteins were deduced. nucleotide and amino acid sequences were compared with those of strain musoke of marburg virus isolated in 1980 in kenya and pur ...19957487490
marburg virus vaccines based upon alphavirus replicons protect guinea pigs and nonhuman primates.marburg virus (mbgv), for which no vaccines or treatments currently exist, causes an acute hemorrhagic fever with a high mortality rate in humans. we previously showed that immunization with either killed mbgv or a glycoprotein (gp) subunit prevented lethal infection in guinea pigs. in the studies reported here, an rna replicon, based upon venezuelan equine encephalitis (vee) virus, was used as a vaccine vector; the vee structural genes were replaced by genes for mbgv gp, nucleoprotein (np), vp4 ...19989813200
[theoretical approaches to the evaluation of antiviral drug effectiveness].it is suggested that (a) ld50 is the measure of the efficacy of antiviral drugs: (b) a disease is characterized by the "barrier" type of a pathological process when in pathogenesis there exists an event whose manifestation largely determines the outcome of a disease (recovery/severe form with a fatal outcome). if the event consists in the penetration of a pathogen into a susceptible cell and the consequent reproduction of an antigen, the value ld50 for man is expressed as the same parameter for ...19937510180
co- and posttranslational modifications and functions of marburg virus proteins. 19999893376
the marburg virus outbreak of 1967 and subsequent episodes. 19999893378
termini of all mrna species of marburg virus: sequence and secondary structure.the 3' and 5' ends of marburg virus (mbg)-specific mrna species have been determined using reverse transcription-pcr, rapid amplification of cdna ends, or the reverse ligation-mediated pcr procedure after removal of cap structures with tobacco acid pyrophosphatase. the polyadenylation sites of all mbg-specific mrnas were strictly conserved and corresponded to the predicted transcriptional stop signals of genomic rna. determination of the 5' ends of the mrna species showed that mrna synthesis sta ...19968806574
differentiation of filoviruses by electron microscopy.cultured monolayers of ma-104, vero 76, sw-13, and dbs-frhl-2 cells were infected with marburg (mbg), ebola-sudan (ebo-s), ebola-zaire (ebo-z), and ebola-reston (ebo-r) viruses (filoviridae, filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these potential human pathogens. replication of each filovirus was seen in all cell systems employed. filoviral particles appeared to enter host cells by endocytosis. filoviruses showed a simi ...19958837880
filovirus activity among selected ethnic groups inhabiting the tropical forest of equatorial africa.seroepidemiological surveys were conducted to determine the frequency and distribution of filovirus activity among selected ethnic groups inhabiting the tropical forests of the central african republic. 427 serum specimens were collected from hunter-gatherers and subsistence farmers living in forest environs in the lobaye district south of the river lobaye and west of the river oubangui. striking serological evidence for filovirus activity was found in both populations. ebola virus appears to be ...19938266403
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