| ebola and marburg viruses: i. some ultrastructural differences between strains when grown in vero cells. | a strain of marburg virus and two strains of ebola virus grown in vero cells were compared by electron microscopy. the outer coat of the marburg virion appeared to be more resistant to erosion by negative staining techniques than that of the epbola strains. marburg virus commonly produced "torus" forms and short filaments; the zaire strain of ebola produced extensive branched forms and very long filaments; the sudan strain of ebola produced shorter, less branched structures but very many aberran ... | 1979 | 94087 |
| [ebola virus three years later (author's transl)]. | sporadic cases and data from serologic surveys give evidence that ebola virus is still active in northern zaïre after the first outbreak in 1976. it is also active in southern sudan where it is, from august 1979, responsible of a new epidemic focus. in addition, serological surveys demonstrate that its dispersion area comprises several other african countries. physicians practising in central africa must be aware of this fact. serological test is necessary to confirm the diagnosis. this confirma ... | 1979 | 119124 |
| ebola and marburg viruses: ii. thier development within vero cells and the extra-cellular formation of branched and torus forms. | the development of marburg virus and the sudanese and zaire strains of ebola virus in vero cells as visualized by electron microscopy is described. despite differences in timing, all three strains appear to pass through identical stages of development. initially there is a large increase in nucleolus material, and viral precursor material arranges itself in spirals and then into tubes. the cells fill with core material, which passes to the plasmalemma, which often proliferates. each virion passe ... | 1979 | 119829 |
| [new african hemorrhagic fevers]. | | 1979 | 392795 |
| measurement of antibodies to ebola virus in human sera from n. w.-zaire. | | 1979 | 395911 |
| [african hemorrhagic fever caused by ebola virus]. | | 1979 | 503930 |
| ebola virus virulence for newborn mice. | | 1979 | 536744 |
| [african hemorrhagic fever as a new problem in medicine]. | | 1979 | 573738 |
| [marburg, ebole and lassa virus infections]. | | 1979 | 574072 |
| [virological and clinical notes on recent viral epidemics in zaïre]. | | 1979 | 574073 |
| [ebola virus reproduction in cell cultures]. | ebola-zaire virus production in vero and bgm cells was studied. the cpe developed in both cell cultures. the cell monolayer destruction by 80-90% was seen at a low multiplicity of infection in 7-8 days after virus inoculation. an overlay composition was developed for virus titration using plaque assay. the plaque production was shown to be directly proportional to the virus dose. the curve of ebola virus production in vero cell culture fluid was determined. at a multiplicity of infection of 0.01 ... | 1992 | 1279896 |
| the envelope glycoprotein of ebola virus contains an immunosuppressive-like domain similar to oncogenic retroviruses. | genomic rna of a zaire strain of ebola virus was cloned, and cdna inserts specific for the glycoprotein gene were isolated and sequenced. the determined sequence has only one open reading frame encoding 318 amino acids and is part of orf-4 on the plus rna strand. the putative transcriptional stop site (3' aauucuuuuu 5') and the transcriptional start site (3' aacuacuucuaauu..5') were identified. computer-assisted comparison of the amino acid sequence of the c-terminal part of protein encoded by o ... | 1992 | 1299611 |
| combined simian hemorrhagic fever and ebola virus infection in cynomolgus monkeys. | simian hemorrhagic fever (shf) virus and a new strain of ebola virus were isolated concurrently in recently imported cynomolgus monkeys (macaca fascicularis) being maintained in a quarantine facility. ebola virus had never been isolated in the u.s. previously and was presumed to be highly pathogenic for humans. a chronology of events including measures taken to address the public health concerns is presented. the clinicopathologic features of the disease were abrupt anorexia, splenomegaly, marke ... | 1992 | 1318446 |
| sequence analysis of the marburg virus nucleoprotein gene: comparison to ebola virus and other non-segmented negative-strand rna viruses. | the first 3000 nucleotides from the 3' end of the marburg virus (mbg) genome were determined from cdna clones produced from genomic rna and mrna. identified in the sequence was a short putative leader sequence at the extreme 3' end, followed by the complete nucleoprotein (np) gene. the 5' end of the np mrna was determined as was the polyadenylation site for the np gene. the transcriptional start (3' uucuucuuauaauu..) and termination (3' ..uaauucuuuuu) signals of the mbg np gene are very similar ... | 1992 | 1538192 |
| enzyme immunosorbent assay for ebola virus antigens in tissues of infected primates. | a sandwich enzyme immunosorbent assay (eia) using a mixture of mouse monoclonal antibodies for antigen capture and polyclonal hyperimmune rabbit anti-ebola virus serum for antigen detection was developed and evaluated on the tissues of monkeys naturally or experimentally infected with strains of ebola viruses. when compared with virus isolation, the antigen detection eia was both sensitive and specific: 44 of 45 (97.7%) liver homogenates and 38 of 41 (92.7%) spleen homogenates that were culture ... | 1992 | 1572982 |
| outbreak of fatal illness among captive macaques in the philippines caused by an ebola-related filovirus. | following the detection of an ebola-like virus in cynomolgus macaques recently imported into the united states from the philippines, studies were initiated to document transmission at export facilities located in the latter country. at one export facility, 52.8% of 161 monkeys that died over a 2.5-month period were shown to be infected with this virus using an enzyme-linked immunosorbent assay to detect antigen in liver homogenates. a case fatality rate of 82.4% was documented for the infected m ... | 1992 | 1621890 |
| seroepidemiological study of filovirus related to ebola in the philippines. | | 1991 | 1671441 |
| virus zoonoses and their potential for contamination of cell cultures. | silent virus infections of laboratory animals present a human health hazard, from direct exposure and from contamination of biological products for human use. here we report two recent examples. in 1989, an outbreak of lymphocytic choriomeningitis virus (lcmv) infections was recognized among workers at a cancer research center after an animal caretaker developed viral meningitis. investigation revealed that multiple tumor cell lines at the facility were infected with lcmv, as were research anima ... | 1991 | 1794619 |
| sequence of the major nucleocapsid protein gene of pneumonia virus of mice: sequence comparisons suggest structural homology between nucleocapsid proteins of pneumoviruses, paramyxoviruses, rhabdoviruses and filoviruses. | the complete nucleotide sequence of gene 3 of pneumonia virus of mice has been determined, and the 5' end of the mrna mapped using a modification of the polymerase chain reaction technique. the gene contains a single open reading frame, beginning with a 5'-proximal aug initiation codon, encoding a polypeptide with a predicted mr of 43141. expression of the gene 3 protein in escherichia coli and in vitro showed that it reacted with virus-specific antiserum and comigrated with the major nucleocaps ... | 1991 | 1848602 |
| preliminary report: isolation of ebola virus from monkeys imported to usa. | an epizootic caused by an ebola-related filovirus and by simian haemorrhagic fever virus began among cynomolgus monkeys in a us quarantine facility after introduction of monkeys from the philippines. this incident, the first in which a filovirus has been isolated from non-human primates without deliberate infection, raises the possibility that cynomolgus monkeys could be a reservoir of ebola virus infection. | 1990 | 1968529 |
| detection of ebola-like viruses by immunofluorescence. | | 1990 | 1979412 |
| rapid identification of ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy. | recent filoviral outbreaks in animal primates have raised public awareness of the potential for filoviruses to become a public health concern; methods that efficiently identify these viruses are therefore of high priority. an indirect immunoelectron microscopy method, which uses homologous guinea pig polyclonal antiserum, successfully identified ebola-related (reston) virus particles in serum and tissue culture fluid specimens with infectivity titres of 300 plaque forming units (pfu) per ml or m ... | 1991 | 2066435 |
| update: ebola-related filovirus infection in nonhuman primates and interim guidelines for handling nonhuman primates during transit and quarantine. | | 1990 | 2104655 |
| update: filovirus infection in animal handlers. | | 1990 | 2107388 |
| update: filovirus infections among persons with occupational exposure to nonhuman primates. | | 1990 | 2109172 |
| update: evidence of filovirus infection in an animal caretaker in a research/service facility. | | 1990 | 2109176 |
| update: filovirus infection associated with contact with nonhuman primates or their tissues. | | 1990 | 2112686 |
| emerging viruses, emerging threat. | | 1990 | 2153314 |
| import rules threaten research on primates. | | 1990 | 2160732 |
| ebola virus. | | 1990 | 2167118 |
| use of immunoelectron microscopy to show ebola virus during the 1989 united states epizootic. | a filovirus, serologically related to ebola virus, was detected by "post-embedment" immunoelectron microscopical examination of ma-104 cells. these had been infected by inoculation with serum samples obtained during the 1989 epizootic in cynomolgus monkeys (macaca fascicularis), imported from the philippines and maintained at reston, virginia, usa, a primate holding facility. the immunoelectron microscopy method, when used in conjunction with standard transmission electron microscopy (tem) of in ... | 1990 | 2229429 |
| filovirus infection in newly imported monkeys. | | 1990 | 2237399 |
| not enough monkey business. | | 1990 | 2237402 |
| ebola virus infection in imported primates--united states. | in late november 1989, ebola virus was isolated from cynomolgus monkeys (macaca fascicularis) imported into the united states from the philippines via amsterdam and new york. during quarantine in a primate facility in virginia, numerous macaques died, some with findings consistent with simian hemorrhagic fever (shf). the us army medical research institute of infectious diseases tested 10 animals and, from 3, isolated shf from tissues and serum; however, 5 other animals of the 10 tested were posi ... | 1990 | 2302743 |
| monkey imports may be curtailed in us. | | 1990 | 2314464 |
| us shuts down monkey trade. | | 1990 | 2320102 |
| imported monkey puzzle. | | 1990 | 2321012 |
| antibody prevalence against haemorrhagic fever viruses in randomized representative central african populations. | between 1985 and 1987, 5,070 randomly selected persons living in 6 central african countries (cameroon, central african republic, chad, congo, equatorial guinea and gabon) were checked for serological evidence of haemorrhagic fever. rural and urban areas were studied, including ecoclimatic zones ranging from dry savana to tropical rain forest. virus-reactive antibodies were found with all antigens tested, and the global prevalence of positive sera was distributed as follows: crimean-congo haemor ... | 1989 | 2505350 |
| ebola virus infection in imported primates--virginia, 1989. | | 1989 | 2511410 |
| antibodies to haemorrhagic fever viruses in madagascar populations. | sera of 381 adult people from 5 areas in madagascar were tested by the indirect immunofluorescence method for antibodies against congo-crimean haemorrhagic fever and rift valley fever viruses (bunyaviridae), ebola (strains zaire and sudan) and marburg viruses (filoviridae), and lassa virus (arenaviridae). the highest prevalence rate was that of ebola virus (4.5%). as no haemorrhagic syndrome has been found associated with this virus, the possible presence of a less pathogenic, antigenically rela ... | 1989 | 2515626 |
| epidemiology of hemorrhagic fever viruses. | twelve distinct viruses associated with hemorrhagic fever in humans are classified among four families: arenaviridae, which includes lassa, junin, and machupo viruses; bunyaviridae, which includes rift valley fever, crimean-congo hemorrhagic fever, and hantaan viruses; filoviridae, which includes marburg and ebola viruses; and flaviviridae, which includes yellow fever, dengue, kyasanur forest disease, and omsk viruses. most hemorrhagic fever viruses are zoonoses, with the possible exception of t ... | 1989 | 2546247 |
| adventitious viral agents in biological products. | the objectives of tests for extraneous agents will be discussed in the light of quality requirements for biological products published over the last twenty years, current developments of novel production methods and products, and past and current virological findings. | 1989 | 2547677 |
| the nucleoprotein gene of ebola virus: cloning, sequencing, and in vitro expression. | genomic and messenger rnas of a zaire strain of ebola virus were cloned, and inserts specific for the nucleoprotein gene were isolated and sequenced. the nucleoprotein gene is located proximal to the 3' end of the genome and is preceeded by a putative leader sequence. the gene begins with the transcriptional start site sequence 3'-uacuccuucuaauu..., and ends with the polyadenylation site sequence 3'-... uaauucuuuuuu. the predicted coding region is 2217 bases in length and encodes a protein that ... | 1989 | 2718390 |
| firsthand clinical observations of hemorrhagic manifestations in ebola hemorrhagic fever in zaire. | about 5 weeks after the beginning of the outbreak of ebola virus fever in yambuku, zaire, several acute cases of the disease were observed. all of those affected had the following common signs and symptoms: sudden onset of high fever, with chills, headache, myalgia, anorexia, nausea, abdominal pain, sore throat, expressionless face, and profound prostration. in some cases, on around the fifth day of the acute phase, the appearance of an exanthematous rash on the trunk announced the hemorrhagic m ... | 1989 | 2749110 |
| seasonal variation in antibodies against ebola virus in kenyan fever patients. | | 1986 | 2871413 |
| identification and analysis of ebola virus messenger rna. | six messenger rna species of ebola virus were identified in infected vero e6 cells. virion rna hybridizes to each of the mrnas, confirming that ebola virus possesses a negative-stranded rna genome. the mrnas are monocristronic transcripts, are synthesized in the presence of actinomycin d, and are polyadenylated. in vitro translation of mrna preparations results in the synthesis of five authentic viral proteins and a putative unglycosylated form of the glycoprotein, demonstrated by immunoprecipit ... | 1987 | 2881398 |
| [hemorrhagic fever viruses. principal epidemiologic aspects]. | | 1987 | 3038354 |
| [serological study of the virus responsible for hemorrhagic fever in an urban population of cameroon]. | a sero-epidemiological study of hemorrhagic fever viruses in a urban population of cameroon. the authors report the results of a sero-epidemiological survey undertaken in a urban population of cameroon and concerning congo, rift (rvf), lassa, ebola, marburg and yellow fever viruses. on 375 human sera tested, 1.06% show antibodies against rvf virus and 1.87% are positive for anti-ebola antibodies thus yielding evidence that these two viruses are present in this area of cameroon. 33.75% have antib ... | 1988 | 3064937 |
| hemorrhagic fever virus infections in an isolated rainforest area of central liberia. limitations of the indirect immunofluorescence slide test for antibody screening in africa. | serum samples from 119 healthy individuals and 106 epilepsy patients inhabiting grand bassa county, liberia, were tested for antibodies to hemorrhagic fever viruses (hfv) by indirect immunofluorescence. e6 vero cells infected with lassa fever virus (las), rift valley fever virus (rvf), congo hemorrhagic fever virus (con), marburg virus (mbg) and the ebola (ebo) virus strains mayinga (may) and boniface (bon) were used as antigen. to obtain reproducible and specific test results sera had to be abs ... | 1986 | 3092415 |
| management of patients with suspected viral hemorrhagic fever. | | 1988 | 3126390 |
| viral hemorrhagic fever antibodies in nigerian populations. | using the immunofluorescence test, a serosurvey for antibodies to five viral agents associated with hemorrhagic febrile infections was conducted with 1,677 human sera from different parts of nigeria. three hundred fifty-seven (21.3%) were positive for lassa virus antibody, while antibodies to rift valley fever virus were detected in 42 (2.5%) of the sera. testing for rift valley fever virus antibody was confirmed by plaque reduction neutralization test. antibodies to ebola and marburg viruses we ... | 1988 | 3128130 |
| physicochemical properties of marburg virus: evidence for three distinct virus strains and their relationship to ebola virus. | the physicochemical and antigenic properties of three groups of marburg (mbg) virus isolates, separated temporally and geographically, were compared to each other and to another member of the same family, ebola (ebo) virus. each mbg isolate contained seven virion proteins, one of which was a glycosylated surface protein. peptide mapping of glycoproteins, nucleoproteins (np) and viral structural protein (vp40) demonstrated extensive sequence conservation in the proteins of viruses isolated over a ... | 1988 | 3404120 |
| pathophysiology of shock and haemorrhage in viral haemorrhagic fevers. | | 1987 | 3433168 |
| [clinico-epidemiologic and laboratory research on hemorrhagic fevers in guinea]. | in 1982-1983, were reported the cases of haemorrhagic fevers among populations living in the madina-ula district of guinea. clinico-epidemiological and serological studies (experimental studies) reveal into presumption of ebola and lassa fever viruses significance in the etiology of the disease outbreaks. antibodies to ebola virus were recognized in 19% from total number of sweating reconvalescent patients with the same clinical features, in order to 8% in healthy local populations. antibodies t ... | 1987 | 3440310 |
| in-vitro infection of chronic lymphocytic leukemia cells by epstein-barr virus (ebv). | we sought to determine the potential of infecting lymphoid cells from patients with chronic leukemia (cll) with epstein-barr virus (ebv) by testing for ebv receptors (ebvr) by flow cytometry, assessing for infectability of these cells by culturing with b95-8-derived virus, and staining for eb nuclear-associated antigens (ebna) at various times post-infection. ebvr were present on 54-91% of lymphoid cells in seven cases of cll and on 46% of prolymphocytic leukemia cells. dynamic changes regarding ... | 1986 | 3512923 |
| [indirect immunoenzyme method for the laboratory diagnosis of lassa and ebola hemorrhagic fevers]. | conditions for performing solid-phase indirect enzyme-immunoassay (speia) for the detection of lassa and ebola virus antigens and antibodies to them using horseradish peroxidase-labeled antispecific globulins were developed. the method is highly sensitive, specific, and reproducible. by this method, antigens of lassa and ebola viruses could be detected in tissue culture fluid of the infected cell cultures and in animal organ suspensions. detection of antibodies to lassa and ebola viruses in huma ... | 1986 | 3524001 |
| viral haemorrhagic fevers. | | 1986 | 3760594 |
| [method for the simultaneous detection of immunofluorescing antibodies to the causative agents of different hemorrhagic fevers]. | | 1985 | 3907144 |
| conservation of the 3' terminal nucleotide sequences of ebola and marburg virus. | the 3' rna base sequences of several marburg (mbg) and ebola (ebo) virus isolates have been determined. a comparison of these 3' terminal noncoding sequences with those of other negative strand rna viruses suggests a unique phylogenic niche for marburg and ebola viruses. the translation initiation site and 35 n-terminal amino acids of the 3' proximal coding gene of a zaire strain of ebola virus was predicted. in addition, putative leader rna sequences preceding the first gene are discussed in te ... | 1986 | 3946083 |
| pathophysiology of shock and hemorrhage in a fulminating viral infection (ebola). | eleven rhesus monkeys were monitored intensively during experimental infection with ebola virus. prominent neutrophilia with left shift and lymphopenia were the earliest abnormalities and were statistically significant by day 4 (p less than .02 and p less than .01, respectively). by day 4 falls in platelet counts were not statistically significant, whereas in vitro platelet aggregation was markedly depressed, progressing rapidly to complete failure by the time of maximum illness. intraplatelet p ... | 1985 | 4045253 |
| descriptive analysis of ebola virus proteins. | the virion proteins of two strains of ebola virus were compared by sds-polyacrylamide gel electrophoresis (page) and radioimmunoprecipitation (rip). seven virion proteins were described; an l (180k), gp (125k), np (104k), vp40 (40k), vp35 (35k), vp30 (30k), and vp24 (24k). the rnp complex of the virus contained the l, the np, and vp30, with vp35 in loose association with them. the gp was the major spike protein, with vp40 and vp24 making up the remaining protein content of the multilayered envel ... | 1985 | 4060597 |
| ultrastructural pathology of experimental ebola haemorrhagic fever virus infection. | the organs of monkeys infected with ebola haemorrhagic fever were examined by light and electron microscopy during the acute stage of the disease. the virus caused focal coagulative necrosis in the liver, spleen, kidney, lung and testis and widespread mild vascular damage. in the brain there was intense congestion, with erythrocyte 'sludging', but no inflammatory reaction. there was significant injury to the microvasculature in all organs. virus replicated in endothelial cytoplasm causing focal ... | 1985 | 4067737 |
| viral haemorrhagic fevers: properties and prospects for treatment and prevention. | | 1984 | 6091539 |
| inactivated vaccine for ebola virus efficacious in guineapig model. | | 1980 | 6108462 |
| marburg and ebola virus antibodies in kenyan primates. | | 1981 | 6113374 |
| marburg-virus disease in kenya. | | 1982 | 6122054 |
| inactivating lassa and marburg/ebola viruses. | | 1982 | 6123862 |
| viral hemorrhagic fevers with hepatic involvement: pathologic aspects with clinical correlations. | | 1982 | 6125993 |
| modifications to indirect immunofluorescence tests on lassa, marburg, and ebola material. | | 1983 | 6131336 |
| haematological and biochemical monitoring of ebola infection in rhesus monkeys: implications for patient management. | patients with severe viral infections such as lassa or ebola may be denied adequate laboratory investigations because of justifiable fears among laboratory staff. this study in monkeys was designed to provide comprehensive haematological and biochemical monitoring in a contained environment during all stages of ebola infection. marked neutrophilia, depletion of lymphocytes, and early failure of platelet aggregation preceded a consumption coagulopathy with a microangiopathic haemolytic anaemia, t ... | 1983 | 6138602 |
| [developments and trends in virology]. | | 1980 | 6154883 |
| a comparative study of strains of ebola virus isolated from southern sudan and northern zaire in 1976. | during the 1976 ebola virus outbreak in sudan, the investigations team gained the impression that fewer haemorrhagic manifestations and few fatalities occurred during the later stages of the epidemic after the virus had undergone several generations in man. this impression was also noted in guinea pigs experimentally infected with sudanese and zairean strains of ebola virus. the virulence of the sudanese isolates was less intense than isolates emanating from zaire. similar findings were seen in ... | 1980 | 6165800 |
| lack of cross reactivity of rhabdovirus antibodies with marburg and ebola antigens in the indirect immunofluorescent antibody test. | | 1982 | 6179483 |
| exotic viral diseases. | marburg virus disease, lassa fever, monkeypox, and ebola virus diseases of humans have all been recognized since 1967. these are examples of some of the exotic virus diseases which through importation may present a potential public health problem in the united states. some of these viruses are also highly hazardous to laboratory and medical personnel. this paper is a review of the general characteristics, the epidemiology, and laboratory diagnosis of the exotic viruses which have been described ... | 1980 | 6246685 |
| nosocomial viral infections: iii. guidelines for prevention and control of exanthematous viruses, gastroenteritis viruses, picornaviruses, and uncommonly seen viruses. | this communication is the third in a four-part series on nosocomial viral infections from the strong memorial hospital. this third article discusses guidelines for prevention and control of exanthematous viruses, gastroenteritis, viruses, adenoviruses and the picornaviruses other than rhinoviruses. several uncommonly seen viruses, such as the virus of creutzfeldt-jakob disease and marburg, ebola, and lassa fever viruses, also are reviewed briefly. | 1981 | 6260699 |
| [epidemic hemorrhagic fevers]. | | 1983 | 6310907 |
| development of an immunofluorescence focus assay for ebola virus. | a 48-h indirect immunofluorescence focus assay for the quantitation of ebola virus was developed, utilizing hela-229 cell monolayers. the dose dependency and the sensitivity of this assay as compared with conventional assays are reported. this indirect immunofluorescence focus assay can be used as a rapid, quantitative test for the detection of ebola virus, an agent from africa known to cause hemorrhagic fever. | 1983 | 6352735 |
| ebola virus disease in southern sudan: hospital dissemination and intrafamilial spread. | between 31 july and 6 october 1979, 34 cases of ebola virus disease (22 of which were fatal) occurred among five families in a rural district of southern sudan; the disease was introduced into four of the families from a local hospital. chains of secondary spread within the family units, accounting for 29 cases resulted from direct physical contact with an infected person. among all persons with such contact in the family setting, those who provided nursing care had a 5.1-fold increased risk of ... | 1983 | 6370486 |
| antibodies against haemorrhagic fever viruses in kenya populations. | human sera from lodwar (77 sera), nzoia (841 sera), masinga (251 sera), laisamis (174 sera) and the malindi/kilifi area (556 sera) in kenya were tested by indirect immunofluorescence for antibodies against marburg, ebola (zaire and sudan strains), congo haemorrhagic fever, rift valley fever and lassa viruses. antibodies against ebola virus, particularly the zaire strain, were detected in all regions and were, over-all, more abundant than antibodies against the other antigens. ebola and marburg a ... | 1983 | 6419422 |
| physicochemical inactivation of lassa, ebola, and marburg viruses and effect on clinical laboratory analyses. | clinical specimens from patients infected with lassa, ebola, or marburg virus may present a serious biohazard to laboratory workers. we have examined the effects of heat, alteration of ph, and gamma radiation on these viruses in human blood and on the electrolytes, enzymes, and coagulation factors measured in laboratory tests that are important in the care of an infected patient. heating serum at 60 degrees c for 1 h reduced high titers of these viruses to noninfectious levels without altering t ... | 1984 | 6490832 |
| radiography in a secure isolation unit. | | 1984 | 6538049 |
| [the epidemic of hemorrhagic fever in zaire (august-november 1976) and its implications]. | | 1983 | 6613302 |
| ebola virus infection in man: a serological and epidemiological survey in the cameroons. | the presence of antibodies to ebola virus among 1,517 apparently healthy persons in five regions of the cameroons was tested using indirect immunofluorescence. a positive rate of 9.7% was found, confirming that the virus circulates in the absence of clinical cases. highest rates were found among pygmies, young adults, and rain forest farmers. | 1983 | 6650749 |
| a probable case of ebola virus haemorrhagic fever in kenya. | | 1983 | 6671431 |
| viral haemorrhagic fever surveillance in kenya, 1980-1981. | following two cases of marburg virus disease in kenya in 1980, viral haemorrhagic fever surveillance was undertaken in western kenya. over a 21-month period investigations, including virus isolation attempts, patient and contact serology, visits to areas where suspected cases occurred, interviewing family members and neighbours of suspected cases and following up any additional illnesses in these areas, were carried out. during the study two cases were found that were likely to have been ebola h ... | 1983 | 6684336 |
| antibody to ebola virus in guinea pigs: tandala, zaire. | a case-control study was conducted to investigate the findings of antibody to ebola virus in the serum of a guinea pig from tandala, zaire. case households, defined by the possession of one or more guinea pigs, were compared to neighboring households without guinea pigs. seven (5.1%) of 138 samples of human sera and 36 (26%) of 138 samples of guinea pig sera had antibody to ebola virus. there was no clustering of seropositivity among humans or guinea pigs within households, nor was there any ass ... | 1982 | 6750007 |
| viral haemorrhagic fever antibodies in zimbabwe schoolchildren. | | 1982 | 6761909 |
| the nasty viruses--lassa, marburg, and ebola. | | 1980 | 6768412 |
| exotic infectious diseases: marburg/ebola/haemorrhagic fevers. | | 1980 | 6770413 |
| [pathogenic microbiology of international communicable diseases]. | | 1980 | 6771443 |
| marburg, ebola and rift valley fever virus antibodies in east african primates. | sera from 464 primates held at four institutes in kenya were tested by indirect immunofluorescence for the presence of antibodies against marburg, ebola, congo haemorrhagic fever, rift valley fever and lassa viruses. four of 136 vervet monkeys were positive for marburg virus antibodies and three of 184 baboons had antibodies against ebola virus. one baboon was positive for marburg virus antibodies. two vervet monkeys, three baboons and one grivet monkey (of 56 tested) had antibodies against rift ... | 1982 | 6810518 |
| biologic differences between strains of ebola virus from zaire and sudan. | | 1983 | 6827142 |
| antigenic analysis of strains of ebola virus: identification of two ebola virus serotypes. | a sensitive radioimmunoassay has been adapted for ebola virus antigens and antibodies to them. it uses 125i-labeled staphylococcal protein a and a specially designed filter manifold. the assay is applicable to the sera of humans and to a wide range of animal sera. virus isolates from two discrete outbreaks of ebola hemorrhagic fever that occurred in 1976 were shown by this assay to be antigenically distinct. this lack of identity was further confirmed by cross-absorbing antisera to each isolate ... | 1983 | 6827143 |
| evidence for two subtypes of ebola virus based on oligonucleotide mapping of rna. | ebola viruses isolated during outbreaks of acute hemorrhagic fever in africa from 1976 to 1979 were examined by t1 oligonucleotide mapping of virion rna. two ebola virus subtypes distinguishable by their oligonucleotide patterns were involved in the outbreaks of the disease during this three-year period. the first type was isolated in zaire in 1976 and again in 1977; the second type caused outbreaks in sudan in 1976 and again in 1979. oligonucleotide patterns of the two groups of ebola viruses ( ... | 1983 | 6827144 |
| comparative analysis of the structural polypeptides of ebola viruses from sudan and zaire. | polypeptide structural analyses were performed by tryptic peptide mapping to assess the relationship between isolates of ebola virus obtained in sudan and zaire. the results of these analyses indicate (1) that the sudan and zaire isolates are unique viral agents, (2) that multiple isolates within each group bear close resemblance to one another, (3) that suggestive evidence exists of conservation of homologous structure in the vp-2 (virion protein no. 2) nucleocapsid proteins of these viruses, a ... | 1983 | 6827145 |
| changing patterns of communicable disease in england and wales. part i--newly recognised diseases. | | 1980 | 7000261 |
| [preliminary note on the presence of antibodies to ebola virus in the human population in the eastern part of the central african republic]. | the authors reported a preliminary serological survey on ebola virus infection in central african republic. they have tested 499 sera samples by using indirect immunofluorescent technique. the positivity with ebola antigen was 3,4% (17 cases). it has been found a high antibody titre (greater than or equal to 1/64) with 3 sera, which reflects a possible recent contact with ebola virus. | 1980 | 7014009 |
| plaque assay for ebola virus. | a plaque assay for ebola virus is reported. the procedure has real potential for future research, although it is less sensitive than indirect fluorescent-antibody and mouse inoculation tests. | 1981 | 7014628 |
| ecology of ebola virus: a first clue? | | 1981 | 7017023 |
| preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys. | a method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to lassa, marburg, and ebola viruses. the technique uses mixtures of specifically infected vero cells placed on teflon-templated microscopy slides. it was found to be as sensitive as the use of monovalent antigens for detection and quantitation of antibodies to these highly hazardous human pathogens. | 1981 | 7031084 |