| sequence analysis of genome segments s4 and s8 of mal de río cuarto virus (mrcv): evidence that the virus should be a separate fijivirus species. | this is the first sequence-based characterization of mal de río cuarto virus (mrcv), currently classified as a variant of maize rough dwarf virus (mrdv) and exclusively found in south america. we sequenced and analyzed genome segments s4 and s8. mrcv s4 coded for a putative 131.67 kda protein while mrcv s8 coded for a putative 68.26 kda protein containing an atp/gtp-binding motif. the 5' and 3' ends of mrcv segments, were 5'aaguuuuu3' and 5'cagcunnnguc3', respectively. prediction of secondary st ... | 2002 | 12209310 |
| sequence and phylogenetic analysis of genome segments s1, s2, s3 and s6 of mal de río cuarto virus, a newly accepted fijivirus species. | mal de río cuarto virus (mrcv) is a newly described species of the genus fijivirus, family reoviridae. the nucleotide sequence of four mrcv genome segments was determined. mrcv s1, s2, s3 and s6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kda, respectively. mrcv s1 encodes a basic protein that contains conserved rna-dependent rna polymerase motifs, and is homologous to rice black streaked dwarf virus (rbsdv), fiji disease virus (fdv) and nilaparvata lugens reovirus (nlrv) pol ... | 2003 | 12606083 |
| sequence analysis of genome segments s5 and s10 of mal de rio cuarto virus (fijivirus, reoviridae). | mal de rio cuarto virus (mrcv) was recently described as a new species of the genus fijivirus, family reoviridae. the nucleotide sequence of two mrcv genome segments was determined. mrcv s5 and s10 were predicted to encode proteins of 106.9 and 63.5 kda respectively. the protein coded by mrcv s5 had 62.8% and 35.7% identity to fijiviruses rbsdv s5 and fdv s5 coded proteins, and contained a rarely reported type-1 c-terminal peroxisomal targeting signal. the protein coded by mrcv s10 had identity ... | 2005 | 15747051 |
| sequencing of the bicistronic genome segments s7 and s9 of mal de río cuarto virus (fijivirus, reoviridae) completes the genome of this virus. | the nucleotide sequences of genomic segments s7 and s9 of mal de río cuarto virus (mrcv, fijivirus group ii) have been determined, thus completing the entire genome sequence of the virus. these segments showed a non-overlapping bicistronic structure, as in other members of the genus. mrcv s7 orf-1 had a length of 1086 bp and encoded a 41.5 kda putative polypeptide, whereas mrcv s7 orf-2 had a length of 930 bp and encoded a 36.8 kda putative polypeptide. proteins of 39 and 20.5 kda were predicted ... | 2007 | 17115303 |
| molecular diversity of ecologically distinct mal de río cuarto virus isolates based on restriction fragment length polymorphism (rflps) and genome sequence analysis of segments 1, 7, 9 and 10. | viruses of the species mal de río cuarto virus (genus fijivirus, family reoviridae) cause significant economic losses in maize in argentina. genetic changes in the virus genome leading to better adaptation to diverse ecological conditions were postulated that would account for the increasing mrcv variability. the genomic differences between mrcv isolates from four ecologically different areas (río cuarto, rc; pergamino, p; jesús maría, jm; and tafí del valle, tv) were studied. rt-pcr-amplified f ... | 2007 | 17370108 |
| a black-streaked dwarf disease on rice in china is caused by a novel fijivirus. | an isolate of a plant reovirus causing severe stunting and dark leaf symptoms on rice from guangdong, china, was similar in virion morphology and serologically related to rice black-streaked dwarf virus (rbsdv). the electrophoretic profiles of genome segments of the two viruses in agarose or polyacrylamide gel were indistinguishable. the four genome segments of the new isolate corresponding to rbsdv s7-s10 were amplified by ligation rt-pcr and sequenced. the size and organization of each genome ... | 2008 | 18820828 |
| mal de río cuarto virus (mrcv) genomic segment s3 codes for the major core capsid protein. | mal de río cuarto virus (mrcv) is a newly described species of the genus fijivirus, family reoviridae. compared with other plant-infecting genus of the family, the function and localization of mrcv and other fijivirus proteins are poorly understood. through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kda. the protein encoded by mrcv s3 genomic segment was expressed as a fusion protein in escherichia coli, ... | 2009 | 19280330 |
| wheat antixenosis, antibiosis, and tolerance to infestation by delphacodes kuscheli (hemiptera: delphacidae), a vector of "mal de río cuarto" in argentina. | "mal de rio cuarto", is the most important virus disease of corn, zea mays l., in argentina. it is caused by the mal de rio cuarto virus (family reoviridae, genus fijivirus. mrcv), which is a persistent virus transmitted by delphacodes kuscheli (fennah 1955) (hemiptera: delphacidae). because corn is not a natural host of d. kuscheli, it has little protection from this pest. in contrast, wheat, triticum aestivum l., is one of the main hosts of this vector and a reservoir of mrcv. the aim of this ... | 2009 | 19886444 |
| novel calicivirus identified in rabbits, michigan, usa. | we report a disease outbreak in a michigan rabbitry of a rabbit calicivirus distinct from the foreign animal disease agent, rabbit hemorrhagic disease virus (rhdv). the novel virus has been designated michigan rabbit calicivirus (mrcv). caliciviruses of the lagovirus genus other than rhdv have not been described in us rabbit populations. the case-fatality rate was 32.5% (65/200). clinical signs included hemorrhage and sudden death, with hepatic necrosis. analysis of viral rna sequence from >95% ... | 2009 | 19961675 |
| immunodetection and subcellular localization of mal de río cuarto virus p9-1 protein in infected plant and insect host cells. | mal de río cuarto virus (mrcv), a member of the genus fijivirus, family reoviridae, has a genome consisting of 10 dsrna segments. the segment 9 (s9) possesses two non-overlapping open reading frames (orf-1 and orf-2) encoding two putative proteins, mrcv p9-1 and mrcv p9-2, both of unknown function. the mrcv s9 orf-1 was rt-pcr amplified, expressed in pet-15b vector, and the recombinant protein produced was used to raise an antiserum in rabbit. western blot with the specific mrcv p9-1 antiserum d ... | 2010 | 20419342 |
| functional and biochemical properties of mal de río cuarto virus (fijivirus, reoviridae) p9-1 viroplasm protein show further similarities to animal reovirus counterparts. | mal de río cuarto virus (mrcv) is a plant virus of the genus fijivirus within the family reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. other members of the family replicate in viral inclusion bodies (vibs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. in this study, the protein coded by the first orf of mrcv segment s9 (p9-1) was shown to establish cytoplasmic inclusion ... | 2010 | 20600394 |
| reference gene selection for gene expression studies using rt-qpcr in virus-infected planthoppers. | planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. the analysis of gene expression in persistently infected planthoppers might unveil the molecular basis of viral transmission. quantitative real-time rt-pcr (rt-qpcr) is currently the most accurate and sensitive method used for quantitative gene expression analysis. in order to normalize the resulting quantitative data, reference genes with constant expression ... | 2011 | 21679431 |
| development of a novel set of gateway-compatible vectors for live imaging in insect cells. | insect genomics is a growing area of research. to exploit fully the genomic data that are being generated, high-throughput systems for the functional characterization of insect proteins and their interactomes are required. in this work, a gateway-compatible vector set for expression of fluorescent fusion proteins in insect cells was developed. the vector set was designed to express a protein of interest fused to any of four different fluorescent proteins [green fluorescent protein (gfp), cyan fl ... | 2011 | 22093064 |
| wolbachia occurrence in planthopper (hemiptera: delphacidae) vectors of cereal viruses in argentina. | maize (zea mays l.) and wheat (triticum aestivum l.) are the most important cereal crops for the argentinean economy and are affected by several diseases. different planthopper species transmit causal agents of some of those diseases, including mal de río cuarto virus, barley yellow striate mosaic virus, and the recently proposed maize yellow striate virus. many planthopper species are sap feeders and therefore are expected to host bacteria providing essential nutrients lacking in the diet. prev ... | 2015 | 26470291 |
| interaction of mal de río cuarto virus (fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay. | mal de río cuarto virus (mrcv) is a member of the fijivirus genus, within the reoviridae family, that replicates and assembles in cytoplasmic inclusion bodies called viroplasms. in this study, we investigated interactions between ten mrcv proteins by yeast two-hybrid (y2h) assays and identified interactions of non-structural proteins p6/p6, p9-2/p9-2 and p6/p9-1. p9-1 and p6 are the major and minor components of the viroplasms respectively, whereas p9-2 is an n-glycosylated membrane protein of u ... | 2017 | 28087398 |
| a molecular framework for the identification of planthopper vectors (hemiptera: delphacidae) of central argentina. | planthoppers are important worldwide crop pests as well as vectors of numerous diseases. different species transmit mal de río cuarto virus, which causes the most economically important corn disease in central argentina. epidemiological studies rely on the accurate identification of the species present in the field. presently, morphological identification of planthoppers requires taxonomic expertise and there are no taxonomic keys for females and nymphs. nevertheless, no molecular protocols are ... | 2015 | 26353811 |
| identification and fine-mapping of a qtl, qmrdd1, that confers recessive resistance to maize rough dwarf disease. | maize rough dwarf disease (mrdd) is a devastating viral disease that results in considerable yield losses worldwide. three major strains of virus cause mrdd, including maize rough dwarf virus in europe, mal de río cuarto virus in south america, and rice black-streaked dwarf virus in east asia. these viral pathogens belong to the genus fijivirus in the family reoviridae. resistance against mrdd is a complex trait that involves a number of quantitative trait loci (qtl). the primary approach used t ... | 2013 | 24079304 |
| in vivo subcellular localization of mal de río cuarto virus (mrcv) non-structural proteins in insect cells reveals their putative functions. | the in vivo subcellular localization of mal de río cuarto virus (mrcv, fijivirus, reoviridae) non-structural proteins fused to gfp was analyzed by confocal microscopy. p5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its pdz binding tkf motif, suggesting that p5-1 interacts with cellular pdz proteins. p5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic c-termini. p7-1 and p7-2 also entered the nucleus and therefore, along ... | 2012 | 22608534 |
| mal de río cuarto virus infection triggers the production of distinctive viral-derived sirna profiles in wheat and its planthopper vector. | plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. this study aims to comparatively assess alterations of small rna (srna) populations in both hosts upon virus infection. for this purpose, we characterized the srna profiles of wheat and planthopper vectors infected by mal de río cuarto virus (mrcv, fijivirus, reoviridae) and quantified virus genome segments by quantitative reverse transcription pcr we pr ... | 2017 | 28539933 |