| gene c2 of the monopartite geminivirus tomato yellow leaf curl virus-china encodes a pathogenicity determinant that is localized in the nucleus. | expression of the tomato yellow leaf curl virus-china (tylcv-c) c2 protein and green fluorescent protein (gfp) fused to the c2 protein (c2-gfp) in nicotiana benthamiana from a potato virus x (pvx) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. the localization of gfp fluorescence in plant cells infected with pvx/c2-gfp and in insect cells transfected with baculovirus expressing c2-gfp indicates that the ... | 2001 | 11551077 |
| tomato yellow leaf curl china virus: monopartite genome organization and agroinfection of plants. | the complete dna sequence (2734 nucleotides) of the monopartite genome of tomato yellow leaf curl china virus (tylccnv), a begomovirus transmitted by the whitefly bemisia tabaci, was determined. the circular genomic dna contains six open reading frames (orfs) encoding proteins of molecular weights >10 kda, of which two (v1 and v2) are located on the virion-sense strand and four (c1, c2, c3 and c4) on the complementary-sense strand. the orfs are comparable to those of other whitefly-transmitted b ... | 2001 | 11682126 |
| mutation of three cysteine residues in tomato yellow leaf curl virus-china c2 protein causes dysfunction in pathogenesis and posttranscriptional gene-silencing suppression. | the nuclear localized c2 protein of the monopartite begomovirus tomato yellow leaf curl virus-china (tylcv-c) contributes to viral pathogenicity. here, we have investigated tylcv-c c2 protein domains that play a role in the phenotype. alignment of the c2 protein with 67 homologues from monopartite and bipartite begomoviruses revealed that a putative zinc-finger motif c36-x1-c38-x7-c46-x6-h53-x4-h58c59 and four potential phosphorylation sites (t52, s61, y68, and s74) are highly conserved. when ex ... | 2002 | 11952122 |
| contribution of the zinc finger to zinc and dna binding by a suppressor of posttranscriptional gene silencing. | the zinc finger c(36)-x1-c(38)-x7-c(46)-x6-h(53) of the nuclearly localized c2 protein of tomato yellow leaf curl virus china is involved in pathogenicity and suppression of posttranscriptional gene silencing (ptgs). here, we demonstrate that the zinc finger is indispensable for the c2 protein to bind zinc and dna. mutation of cysteine residue c(36), c(38), or c(46) reduced the zinc and dna binding capacity of c2 protein. when expressed from potato virus x, all three mutants, c2-c(36)r, c2-c(38) ... | 2003 | 12477872 |
| characterization of dnabeta associated with begomoviruses in china and evidence for co-evolution with their cognate viral dna-a. | eighteen samples of begomoviruses isolated from tobacco, tomato and weed species in yunnan, china were found to be associated with dnabeta molecules, for which the complete nucleotide sequences were found to contain 1333-1355 nt. the 18 dnabeta molecules identified consist of three main types, each associated with a different begomovirus species: 72-99 % nucleotide identity was found within one type, but only 39-57 % identity was found between types. all the dnabeta molecules reported here and e ... | 2003 | 12533720 |
| functional characterization of the nuclear localization signal for a suppressor of posttranscriptional gene silencing. | the nucleus-localized c2 protein of tomato yellow leaf curl virus-china (tylcv-c) is an active suppressor of posttranscriptional gene silencing (ptgs). consistently, infection with tylcv-c resulted in ptgs arrest in plants. the c2 protein possesses a functional, arginine-rich nuclear localization signal within the basic amino acid-rich region (17)kvqhriakkttrrrr(31). when expressed from potato virus x, c2-rrrr(31)dvgg (in which the four consecutive arginine residues (28)rrrr(31) were replaced wi ... | 2003 | 12768021 |
| a modified viral satellite dna that suppresses gene expression in plants. | dnabeta is a type of single-stranded (ss) circular satellite dna found in association with monopartite-genome begomoviruses, such as tomato yellow leaf curl china virus isolate y10 (tylccnv-y10). y10 dnabeta is required for symptom expression in plants but depends on tylccnv-y10 genomic dna (dna-a) for replication and encapsidation. when we converted dnabeta into a gene-silencing vector (modified dnabeta (dnambeta)) by replacing its c1 open-reading frame (orf) with a multiple cloning site (mcs), ... | 2004 | 15144385 |
| a dnabeta associated with tomato yellow leaf curl china virus is required for symptom induction. | we report here that all 25 isolates of tomato yellow leaf curl china virus (tylccnv) collected from tobacco, tomato, or siegesbeckia orientalis plants in different regions of yunnan province, china, were associated with dnabeta molecules. to investigate the biological role of dnabeta, full-length infectious clones of viral dna and dnabeta of tylccnv isolate y10 (tylccnv-y10) were agroinoculated into nicotiana benthamiana, nicotiana glutinosa, nicotiana. tabacum samsun (nn or nn), tomato, and pet ... | 2004 | 15564504 |
| molecular characterization of tomato-infecting begomoviruses in yunnan, china. | the importance of diseases of tomato caused by begomoviruses is increasing worldwide. here, we report that several begomoviruses are associated with tomato leaf curl disease in yunnan province, china. 14 tomato samples showing leaf curl symptoms were collected in three districts in yunnan, and they fell into four groups according to their reaction with a panel of 16 monoclonal antibodies in tas-elisa. comparison of partial dna-a sequences amplified with degenerate primers confirmed the existence ... | 2004 | 15593415 |
| expression of a begomoviral dnabeta gene in transgenic nicotiana plants induced abnormal cell division. | an increasing number of monopartite begomoviruses are being identified that a satellite molecule (dnabeta) is required to induce typical symptoms in host plants. dnabeta encodes a single gene (termed betac1) encoded in the complementary-sense. we have produced transgenic nicotiana benthamiana and n. tabacum plants expressing the betac1 gene of a dnabeta associated with tomato yellow leaf curl china virus (tylccnv), under the control of the cauliflower mosaic virus 35s promoter. transgenic plants ... | 2005 | 15633241 |
| pathogenicity and stability of a truncated dnabeta associated with tomato yellow leaf curl china virus. | dnabeta molecules are single-stranded satellite dna associated with monopartite begomoviruses (family geminiviridae). dnabeta possesses a c1 gene on the complementary strand, which has a conserved position and size. to better understand the function of c1 gene in virus infection, a c1 deletion dnabeta associated with a tomato yellow leaf curl china virus (tylccnv) isolate was constructed. co-agroinoculation with tylccnv showed the truncated dnabeta was infectious in nicotiana benthamiana and n. ... | 2005 | 15763146 |
| a begomovirus dnabeta-encoded protein binds dna, functions as a suppressor of rna silencing, and targets the cell nucleus. | our previous results demonstrated that the dnabeta satellite (y10beta) associated with tomato yellow leaf curl china virus y10 isolate (tylccnv-y10) is essential for induction of leaf curl symptoms in plants and that transgenic expression of its betac1 gene in nicotiana plants induces virus-like symptoms. in the present study, in vitro dna binding activity of the betac1 proteins of y10beta and dnabeta (y35beta) found in the tobacco curly shoot virus y35 isolate (tbcsv-y35) were studied following ... | 2005 | 16051868 |
| phloem specific promoter from a satellite associated with a dna virus. | dnabeta is a satellite molecule associated with some monopartite begomoviruses and encodes a single gene (betac1), which is highly conserved in position and size among dnabeta molecules. a 955 nt fragment of tomato yellow leaf curl china virus (tylccnv) dnabeta, upstream of the translation start site of betac1 gene was tested for its promoter activity with gus as a reporter gene. analysis of beta-glucuronidase (gus) activity following transient expression assays indicated that the 955 nt fragmen ... | 2006 | 16169625 |
| a modified viral satellite dna-based gene silencing vector is effective in association with heterologous begomoviruses. | we have previously reported effective gene silencing of a transgene and endogenous plant genes in tobacco and tomato plants using a modified viral satellite dna associated with tomato yellow leaf curl china virus (tylccnv). in this study, we constructed a similar gene silencing vector (dnadeltac12beta) based on the satellite dnabeta associated with tobacco curly shoot virus (tbcsv) by replacing its betac1 gene with a multiple cloning site. strong and stable silencing of cognate genes was achieve ... | 2006 | 16417940 |
| [genomic characterization of tomato yellow leaf curl china virus and its associated satellite dna infecting tobacco in guangxi]. | the virus isolates g102 and g103 were collected from tobacco showing leaf curl symptoms in jingxi, guangxi province. a 0.5kb fragment was obtained by pcr using primers specific to geminivirus genome. the nucleotide sequence identity of the partial dna-a sequence between the two isolates was over 99%. the complete dna-a sequence of g102 was determined to be 2728 nucleotides. comparisons showed that the dna-a of g102 had the highest sequence identity (96.5%) with that of tomato yellow leaf curl ch ... | 2006 | 16933601 |
| vector-virus mutualism accelerates population increase of an invasive whitefly. | the relationships between plant viruses, their herbivore vectors and host plants can be beneficial, neutral, or antagonistic, depending on the species involved. this variation in relationships may affect the process of biological invasion and the displacement of indigenous species by invaders when the invasive and indigenous organisms occur with niche overlap but differ in the interactions. the notorious invasive b biotype of the whitefly complex bemisia tabaci entered china in the late 1990s an ... | 2007 | 17264884 |
| efficient gene silencing induction in tomato by a viral satellite dna vector. | virus-induced gene silencing (vigs) in tomato (lycopersicon esculentum mill.) is currently routinely analysed using tobacco rattle virus (trv)-based vector. we recently reported a new vector system modified from dna beta (dnam beta) of tomato yellow leaf curl china virus (tylccnv) for vigs analysis in solanaceous species including tomato. here, we describe dnam beta-induced gene silencing in tomato. we found that dnam beta-induced gene silencing was initiated from vascular tissues, and later sca ... | 2007 | 17289205 |
| genetic structure and population variability of tomato yellow leaf curl china virus. | geminiviruses have circular single-stranded dna genomes and are important pathogens in tropical and subtropical regions, but their population diversity and variability are poorly understood. here, we have investigated variations accumulating in tomato yellow leaf curl china virus (tylccnv), a geminivirus in the genus begomovirus of the family geminiviridae. the population variation was analyzed in a naturally infected tomato (solanum lycopersicom) plant and in nicotiana benthamiana and tomato pl ... | 2007 | 17376922 |
| pathogenicity of a naturally occurring recombinant dna satellite associated with tomato yellow leaf curl china virus. | recombinant dna beta molecules (recdna-abeta) comprising parts of dna a and dna beta associated with tomato yellow leaf curl china virus (tylccnv) have been identified in naturally infected tobacco plants. several examples of the recombinant dna have been cloned and characterized by sequence analysis. all are approximately half the size of tylccnv genomic dna, and all contain the betac1 gene and the a-rich region from tylccnv dna beta as well as intergenic region sequences and the 5' terminus of ... | 2008 | 18089755 |
| molecular identification and the complete nucleotide sequence of tylcv isolate from shanghai of china. | tomato yellow leaf curl virus (tylcv) belongs to the genus begomovirus, family geminiviridae. it is transmitted by the whitefly bemisia tabaci. a pair of primers was designed according to the specific sequence of the tylcv gene and used in the pcr detection of the virus in the infected tissues of tomato grown in the shanghai area of china. dna was extracted from leaves, fruits, seeds, and roots of infected tomato plants separately. the results showed that the target fragment of about 570 bp coul ... | 2008 | 18373188 |
| use of the modified viral satellite dna vector to silence mineral nutrition-related genes in plants: silencing of the tomato ferric chelate reductase gene, fro1, as an example. | virus-induced gene silencing (vigs) is potentially an attractive reverse-genetics tool for studies of plant gene function, but whether it is effective in silencing mineral nutritional-related genes in roots has not been demonstrated. here we report on an efficient vigs system that functions in tomato roots using a modified viral satellite dna (dnambeta) associated with tomato yellow leaf curl china virus (tylccnv). a cdna fragment of the ferric chelate reductase gene (frol) from tomato was inser ... | 2008 | 18785585 |
| betac1, the pathogenicity factor of tylccnv, interacts with as1 to alter leaf development and suppress selective jasmonic acid responses. | viruses induce pathogenic symptoms on plants but the molecular basis is poorly understood. here, we show that transgenic arabidopsis expressing the pathogenesis protein betac1 of tomato yellow leaf curl china virus (tylccnv), a geminivirus, can phenocopy to a large extent disease symptoms of virus-infected tobacco plants in having upward curled leaves, radialized leaves with outgrowth tissues from abaxial surfaces, and sterile flowers. these morphological changes are paralleled by a reduction in ... | 2008 | 18794352 |
| efficient virus-induced gene silencing in plants using a modified geminivirus dna1 component. | virus-induced gene silencing (vigs) is currently recognized as a powerful reverse genetics tool for application in functional genomics. dna1, a satellite-like and single-stranded dna molecule associated with begomoviruses (family geminiviridae), has been shown to replicate autonomously but requires the helper virus for its dissemination. we developed a vigs vector based on the dna1 component of tobacco curly shoot virus (tbcsv), a monopartite begomovirus, by inserting a multiple cloning site bet ... | 2009 | 19175519 |
| trans-replication of, and competition between, dna beta satellites in plants inoculated with tomato yellow leaf curl china virus and tobacco curly shoot virus. | tomato yellow leaf curl china virus (tylccnv) y10 isolate (y10) and tobacco curly shoot virus (tbcsv) y35 isolate (y35), both obtained from yunnan province, were each found to be associated with a distinct species of satellite dna (dna beta). here, we demonstrate that both y10 dna beta (y10beta) and y35 dna beta (y35beta) were stably trans-replicated by the noncognate begomovirus, although the noncognate dna beta accumulated in plants at a lower level than did the cognate dna beta. in nicotiana ... | 2009 | 19453231 |
| genetic determinants of symptoms on viral dna satellites. | begomovirus-dna-beta disease complexes induce different symptom phenotypes in their hosts. to investigate the genetic determinants of the phenotypic differences, nicotiana spp. and tomato plants were inoculated with infectious clones of tobacco curly shoot virus (tbcsv)/tbcsv dna-beta (tbcsb) and tomato yellow leaf curl china virus (tylccnv)/tylccnv dna-beta (tylccnb) pseudorecombinants and showed that tylccnb induced characteristic vein-thickening and enation symptoms, while tbcsb only slightly ... | 2009 | 19542327 |
| molecular characterization and pathogenicity of tomato yellow leaf curl virus in china. | several tomato production regions in china were surveyed for tomato yellow leaf curl disease (tylcd), and 31 tomato leaf samples showing tylcd-like symptoms were collected. the partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (tylcv) was detected in shanghai, zhejiang, jiangsu shandong and hebei provinces of china. the tylcv isolates found in china share high sequence identity ([98%) and have more than 97% sequence identity with tylcvil[ il:reo] ( ... | 2009 | 19590945 |
| viral infection of tobacco plants improves performance of bemisia tabaci but more so for an invasive than for an indigenous biotype of the whitefly. | the ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. we examined the transmission of tomato yellow leaf curl china virus (tylccnv) by the invasive q biotype and the indigenous zhj2 biotype of the whitefly bemisia tabaci, a plant viral vector, as well as the influence of tylccnv-infection of plants on the performance of the two whitefly biotypes. both whitefly biotypes were able to acquire viruses from infected plants an ... | 2010 | 20043350 |
| differential roles of geminivirus rep and ac4 (c4) in the induction of necrosis in nicotiana benthamiana. | summary the replication-associated protein (rep) of two distinct begomoviruses, the bipartite african cassava mosaic virus (acmv) and the monopartite tomato yellow leaf curl virus-china (tylcv-c), elicits a reaction resembling a hypersensitive response (hr), associated with the induction of local necrosis and a systemic burst of hydrogen peroxide production, when expressed from a potato virus x vector in nicotiana benthamiana. transient expression of the acmv rep after agrobacterium infiltration ... | 2002 | 20569353 |
| tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector. | tomato yellow leaf curl disease (tylcd) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate regions of the world. here, we focus on the interactions through recombination between the different begomovirus species causing tylcd, provide an overview of the interactions with the cellular genes involved in viral replication, and highlight recent progress on the relationships between these viruses and their vector, the whitefly bemisia tabaci. t ... | 2010 | 20618703 |
| an invasive whitefly feeding on a virus-infected plant increased its egg production and realized fecundity. | plant-pathogenic begomoviruses have a complex association with their insect vectors. the interactions of begomoviruses and reproduction of their vectors are poorly understood. bemisia tabaci is known to transmit many begomoviruses, and the spread of b. tabaci, especially the b and q 'biotypes', has been accompanied by the epidemics of begomoviruses. one of these identified disease-causing agents was tomato yellow leaf curl china virus (tylccnv). | 2010 | 20676356 |
| characterization of alphasatellites associated with monopartite begomovirus/betasatellite complexes in yunnan, china. | alphasatellites are single-stranded molecules that are associated with monopartite begomovirus/betasatellite complexes. | 2010 | 20678232 |
| global analysis of the transcriptional response of whitefly to tomato yellow leaf curl china virus reveals the relationship of coevolved adaptations. | the begomoviruses are the largest and most economically important group of plant viruses transmitted exclusively by the whitefly bemisia tabaci in a circulative, persistent manner. the circulation of the viruses within the insect vectors involves complex interactions between virus and vector components; however, the molecular mechanisms of these interactions remain largely unknown. here we investigated the transcriptional response of the invasive b. tabaci middle east-asia minor 1 species to tom ... | 2011 | 21270146 |
| characterization of small interfering rnas derived from the geminivirus/betasatellite complex using deep sequencing. | small rna (srna)-guided rna silencing is a critical antiviral defense mechanism employed by a variety of eukaryotic organisms. although the induction of rna silencing by bipartite and monopartite begomoviruses has been described in plants, the nature of begomovirus/betasatellite complexes remains undefined. | 2011 | 21347388 |
| Suppression of methylation-mediated transcriptional gene silencing by ßC1-SAHH protein interaction during geminivirus-betasatellite infection. | DNA methylation is a fundamental epigenetic modification that regulates gene expression and represses endogenous transposons and invading DNA viruses. As a counter-defense, the geminiviruses encode proteins that inhibit methylation and transcriptional gene silencing (TGS). Some geminiviruses have acquired a betasatellite called DNA ß. This study presents evidence that suppression of methylation-mediated TGS by the sole betasatellite-encoded protein, ßC1, is crucial to the association of Tomato y ... | 2011 | 22028660 |
| molecular characterization of two sweepoviruses from china and evaluation of the infectivity of cloned splcv-js in nicotiana benthamiana. | sweepoviruses are important begomoviruses that infect ipomoea plants worldwide and cause sweet potato yield losses and cultivar decline. two sweepoviruses, sweet potato leaf curl virus-jiangsu (splcv-js) and sweet potato leaf curl china virus-zhejiang (splccnv-zj), were cloned from diseased sweet potato plants collected in the jiangsu and zhejiang provinces of china. sequence characterization and phylogenetic analysis demonstrated that both are typical monopartite begomoviruses and have close re ... | 2011 | 22179901 |
| V2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing suppressor. | The V2 protein of Tomato yellow leaf curl China virus (TYLCCNV) was identified as an RNA silencing suppressor by Agrobacterium-mediated co-infiltration. The V2 protein could inhibit local RNA silencing, systemic RNA silencing of the green fluorescent protein (GFP) gene and the spread of a systemic GFP RNA silencing signal. However, the V2 could not interfere with the cell-to-cell spread of RNA silencing. Subcellular localization assay indicated that the V2 protein was distributed in the cytoplas ... | 2011 | 21893116 |
| begomovirus-whitefly mutualism is achieved through repression of plant defences by a virus pathogenicity factor. | plant-mediated interactions between herbivorous arthropods and pathogens transmitted by herbivores are important determinants of the population dynamics of both types of organisms in the field. the role of plant defence in mediating these types of tripartite interactions have been recognized but rarely examined especially at the physiological and molecular levels. our previous work shows that a worldwide invasive whitefly can establish mutualism with the begomovirus tomato yellow leaf curl china ... | 2012 | 22269032 |
| suppression of terpenoid synthesis in plants by a virus promotes its mutualism with vectors. | vectors often perform better on plants infected with pathogens, and this promotes the spread of pathogens. however, few studies have examined how plant defensive compounds mediate such mutualistic relationships. although tobacco plants are relatively poor host plants for the whitefly bemisia tabaci, tobacco's suitability to the whitefly was substantially increased when infected by the begomovirus tomato yellow leaf curl china virus. the change in suitability was associated with induced terpenoid ... | 2012 | 23279824 |
| microrna profiling of the whitefly bemisia tabaci middle east-aisa minor i following the acquisition of tomato yellow leaf curl china virus. | the begomoviruses are the largest and most economically important group of plant viruses exclusively vectored by whitefly (bemisia tabaci) in a circulative, persistent manner. during this process, begomoviruses and whitefly vectors have developed close relationships and complex interactions. however, the molecular mechanisms underlying these interactions remain largely unknown, and the microrna profiles for viruliferous and nonviruliferous whiteflies have not been studied. | 2016 | 26837429 |
| a viral satellite dna vector-induced transcriptional gene silencing via dna methylation of gene promoter in nicotiana benthamiana. | virus-induced gene silencing (vigs) has been widely used for plant functional genomics study at the post-transcriptional level using various dna or rna viral vectors. however, while virus-induced transcriptional gene silencing (vitgs) via dna methylation of gene promoter was achieved using several plant rna viral vectors, it has not yet been done using a satellite dna viral vector. in this study, a viral satellite dna associated with tomato yellow leaf curl china virus (tylccnv), which has been ... | 2016 | 27422476 |
| autophagy functions as an antiviral mechanism against geminiviruses in plants. | autophagy is an evolutionarily conserved process that recycles damaged or unwanted cellular components, and has been linked to plant immunity. however, how autophagy contributes to plant immunity is unknown. here we reported that the plant autophagic machinery targets the virulence factor βc1 of cotton leaf curl multan virus (clcumuv) for degradation through its interaction with the key autophagy protein atg8. a v32a mutation in βc1 abolished its interaction with nbatg8f, and virus carrying βc1( ... | 2017 | 28244873 |
| molecular identification of tobacco leaf curl disease in sichuan province of china. | tobacco leaf curl disease (tlcd) is caused by begomoviruses in geminiviridae, and infected plants exhibit leaf thickening, downward leaf curling, vein swelling as well as stunting symptoms. it is one of the economically important diseases in tropical and subtropical tobacco-growing areas. seven monopartite begomoviruses have been identified causing tlcd in china. | 2016 | 26738931 |
| tobacco ring e3 ligase ntrfp1 mediates ubiquitination and proteasomal degradation of a geminivirus-encoded βc1. | the βc1 protein encoded by the tomato yellow leaf curl china virus-associated betasatellite functions as a pathogenicity determinant. to better understand the molecular basis whereby βc1 functions in pathogenicity, a yeast two-hybrid screen of a tobacco cdna library was carried out using βc1 as the bait. the screen revealed that βc1 interacts with a tobacco ring-finger protein designated ntrfp1, which was further confirmed by the bimolecular fluorescence complementation and co-immunoprecipitatio ... | 2016 | 27018391 |
| agroinfection of sweet potato by vacuum infiltration of an infectious sweepovirus. | sweepovirus is an important monopartite begomovirus that infects plants of the genus ipomoea worldwide. development of artificial infection methods for sweepovirus using agroinoculation is a highly efficient means of studying infectivity in sweet potato. unlike other begomoviruses, it has proven difficult to infect sweet potato plants with sweepoviruses using infectious clones. a novel sweepovirus, called sweet potato leaf curl virus-jiangsu (splcv-js), was recently identified in china. in addit ... | 2014 | 24903591 |
| a novel dna motif contributes to selective replication of a geminivirus-associated betasatellite by a helper virus-encoded replication-related protein. | rolling-circle replication of single-stranded genomes of plant geminiviruses is initiated by sequence-specific dna binding of the viral replication-related protein (rep) to its cognate genome at the replication origin. monopartite begomovirus-associated betasatellites can be trans replicated by both cognate and some noncognate helper viruses, but the molecular basis of replication promiscuity of betasatellites remains uncharacterized. earlier studies showed that when tomato yellow leaf curl chin ... | 2015 | 26656709 |
| a viral satellite dna vector (tylccnv) for functional analysis of mirnas and sirnas in plants. | with experimental and bioinformatical methods, numerous small rnas, including micrornas (mirnas) and short interfering rnas (sirnas), have been found in plants, and they play vital roles in various biological regulation processes. however, most of these small rnas remain to be functionally characterized. until now, only several viral vectors were developed to overexpress mirnas with limited application in plants. in this study, we report a new small rna overexpression system via viral satellite ... | 2017 | 28228536 |
| itraq analysis of the tobacco leaf proteome reveals that rna-directed dna methylation (rddm) has important roles in defense against geminivirus-betasatellite infection. | geminiviruses have caused serious losses in crop production. to investigate the mechanisms underlying host defenses against geminiviruses, an isobaric tags for relative and absolute quantification (itraq)-based quantitative proteomic approach was used to explore the expression profiles of proteins in nicotiana benthamiana (n. benthamiana) leaves in response to tomato yellow leaf curl china virus (tylccnv) with its betasatellite (tylccnb) at an early phase. in total, 4155 proteins were identified ... | 2017 | 27989946 |
| rna interference of an antimicrobial peptide, btdef, reduces tomato yellow leaf curl china virus accumulation in the whitefly bemisia tabaci. | the whitefly bemisia tabaci (gennadius) is considered one of the main pests for agriculture. one important problem with the whitefly is its notorious status as a vector for plant viruses, primarily begomoviruses. we have previously identified a defensin-like antimicrobial peptide, btdef, from the whitefly b. tabaci meam1. however, the function of btdef in the immune system of the insect vector and begomovirus transmission has yet to be explored. | 2016 | 27804213 |
| determinants of disease phenotype differences caused by closely-related isolates of begomovirus betasatellites inoculated with the same species of helper virus. | tomato yellow leaf curl china virus (tylccnv) is a monopartite begomovirus associated with different betasatellites. in this study, we investigate two different isolates of tomato yellow leaf curl china betasatellite (tylccnb) to determine what features of the viral genome are required for induction of characteristic phenotypic differences between closely-related betasatellite. when co-agroinoculated with tylccnv into nicotiana spp. and tomato plants, tylccnb-y25 induced only leaf curling on all ... | 2015 | 26389936 |
| differential profiles of direct and indirect modification of vector feeding behaviour by a plant virus. | plant viruses interact with their insect vectors directly and indirectly via host plants, and this tripartite interaction may produce fitness benefits to both the vectors and the viruses. our previous studies show that the middle east-asia minor 1 (meam1) species of the whitefly bemisia tabaci complex improved its performance on tobacco plants infected by the tomato yellow leaf curl china virus (tylccnv), which it transmits, although virus infection of the whitefly per se reduced its performance ... | 2015 | 25567524 |
| virulence factors of geminivirus interact with myc2 to subvert plant resistance and promote vector performance. | a pathogen may cause infected plants to promote the performance of its transmitting vector, which accelerates the spread of the pathogen. this positive effect of a pathogen on its vector via their shared host plant is termed indirect mutualism. for example, terpene biosynthesis is suppressed in begomovirus-infected plants, leading to reduced plant resistance and enhanced performance of the whiteflies (bemisia tabaci) that transmit these viruses. although begomovirus-whitefly mutualism has been k ... | 2014 | 25490915 |
| specific cells in the primary salivary glands of the whitefly bemisia tabaci control retention and transmission of begomoviruses. | the majority of plant viruses are vectored by arthropods via persistent-circulative or noncirculative transmission. previous studies have shown that specific binding sites for noncirculative viruses reside within the stylet or foregut of insect vectors, whereas the transmission mechanisms of circulative viruses remain ambiguous. here we report the critical roles of whitefly primary salivary glands (psgs) in the circulative transmission of two begomoviruses. the middle east asia minor 1 (meam1) s ... | 2014 | 25210181 |
| a diverse set of mirnas responsive to begomovirus-associated betasatellite in nicotiana benthamiana. | roles of micrornas (mirnas) and short interfering rnas (sirnas) in biotic stress responses, e.g., viral infection, have been demonstrated in plants by many studies. tomato yellow leaf curl china virus (tylccnv) is a monopartite begomovirus that can systemically infect solanaceae plants, and induces leaf curling, yellowing and enation symptoms when co-inoculated with a betasatellite (tylccnb). the released genome sequence of nicotiana benthamiana provides an opportunity to identify mirnas and sir ... | 2014 | 24618068 |
| molecular characterization of soluble and membrane-bound trehalases of the whitefly, bemisia tabaci. | trehalases (tres) have been demonstrated to be the key enzymes that are involved in various trehalose-associated physiological processes in insects. however, little attention has been devoted to the tres in the whitefly, bemisia tabaci. in this study, a soluble tre (bttre-1) and a membrane-bound tre (bttre-2) were cloned in the invasive cryptic species middle east-asia minor 1 (meam1) of the whitefly b. tabaci complex. alignment of deduced amino acids sequences of both bttres revealed that they ... | 2014 | 24610752 |
| feeding on a begomovirus-infected plant enhances fecundity via increased expression of an insulin-like peptide in the whitefly, meam1. | the middle east-minor 1 cryptic species (meam1), bemisia tabaci (gennadius) is a globally invasive pest. it spreads widely due to its high fecundity and mutualistic interactions with the virus they vector. feeding on virus (tomato yellow leaf curl china virus, tylccnv)-infected host plants improves their fecundity, however, the key factor regulating the signaling transduction in reproduction of whitefly remains to be identified. here, we cloned a full length cdna encoding an insulin-like peptide ... | 2014 | 24532462 |
| suppression of rna silencing by a plant dna virus satellite requires a host calmodulin-like protein to repress rdr6 expression. | in plants, rna silencing plays a key role in antiviral defense. to counteract host defense, plant viruses encode viral suppressors of rna silencing (vsrs) that target different effector molecules in the rna silencing pathway. evidence has shown that plants also encode endogenous suppressors of rna silencing (esrs) that function in proper regulation of rna silencing. the possibility that these cellular proteins can be subverted by viruses to thwart host defense is intriguing but has not been full ... | 2014 | 24516387 |
| detoxification activity and energy cost is attenuated in whiteflies feeding on tomato yellow leaf curl china virus-infected tobacco plants. | the begomovirus tomato yellow leaf curl china virus (tylccnv) can benefit its vector, the whitefly bemisia tabaci, through suppressing the defences of their shared host plants. however, the mechanisms of this vector-virus mutualism remain largely unknown on the insect side of the interaction. here, we compared the transcriptional profiles of female adult whiteflies of b. tabaci middle east-asia minor 1 feeding on tylccnv-free and tylccnv-infected tobacco plants using the next-generation sequenci ... | 2013 | 23889516 |
| a recombinant begomovirus resulting from exchange of the c4 gene. | a begomovirus isolated from malvastrum coromandelianum and tomato originating from yunnan province (china) was shown to be representative of a new begomovirus species, for which the name tomato leaf curl yunnan virus (tlcynv) is proposed. tlcynv has high levels of sequence identity to tomato yellow leaf curl china virus (tylccnv) across the whole genome, except for sequences encompassing the c4 gene. agrobacterium-mediated inoculation showed tlcynv to be highly infectious to a range of plant spe ... | 2013 | 23720217 |
| effects of the virus satellite gene βc1 on host plant defense signaling and volatile emission. | tomato yellow leaf curl china virus spreads together with its invasive vector, the silverleaf whitefly b biotype, which exhibits higher growth rates on infected plants. previous studies indicate that the virus satellite gene βc1 accounts for the visible symptoms of infection and inhibits the constitutive expression of jasmonic acid (ja)--a phytohormone involved in plant defense against whiteflies--and of some ja-regulated genes. here we present new details of the effects of on plant signaling an ... | 2013 | 23299332 |
| enhanced vitellogenesis in a whitefly via feeding on a begomovirus-infected plant. | the meam1 (b biotype) bemisia tabaci (gennadius) is one of the most widespread and damaging whitefly cryptic species. our previous studies discovered that the meam1 whitefly indirectly benefits from interactions with the tomato yellow leaf curl china virus (tylccnv) via accelerated ovarian development and increased fecundity. however, the physiological mechanism of begomoviruse-infected plants acting on the reproduction of the insect vector was unknown. | 2012 | 22937062 |
| mimic phosphorylation of a βc1 protein encoded by tylccnb impairs its functions as a viral suppressor of rna silencing and a symptom determinant. | phosphorylation of the βc1 protein encoded by the betasatellite of tomato yellow leaf curl china virus (tylccnb-βc1) by snf1-related protein kinase 1 (snrk1) plays a critical role in defense of host plants against geminivirus infection in nicotiana benthamiana however, how phosphorylation of tylccnb-βc1 impacts its pathogenic functions during viral infection remains elusive. in this study, we identified two additional tyrosine residues in tylccnb-βc1 that are phosphorylated by snrk1. the effects ... | 2017 | 28539450 |