| antifungal activity of crotalus durissus cumanensis venom. | the susceptibility to crotalus venom of 14 yeast and 10 mould fungal isolates was assessed. this venom was tested in a standardized well diffusion test, using 400 microg/20 microl well. the percentage of susceptibility to yeast isolates was 78.6% (> 8 mm); that for filamentous isolates was 50% (> 8 mm). | 2002 | 11856432 |
| individual venom variability in the south american rattlesnake crotalus durissus cumanensis. | crotalus durissus cumanensis snake venoms from different venezuelan regions, showed biochemical and hemostatic variations. fibrino(geno)lytic, hemorrhagic and procoagulant activities and gel-filtration chromatography and sds-page profiles were analyzed. differences were observed in fibrinolytic activity: kallikrein-like amidolytic activity was highest in venoms of santa teresa, and margarita. lagunetica and carrizales venoms showed the maximum fibrin lysis. the highest hemorrhagic activity was s ... | 2007 | 17482229 |
| alterations in the ultrastructure of cardiac autonomic nervous system triggered by crotoxin from rattlesnake (crotalus durissus cumanensis) venom. | this study explored the toxic effects of crotoxin isolated from crotalus durissus cumanensis venom on the ultrastructure of mice cardiac autonomic nervous system. mice were intravenously injected with saline (control group) and crotoxin diluted in saline venom (study group) at a dose of 0.107 mg/kg mouse body weight. samples from the inter-ventricular septum were prepared for electron microscopy after 6 h (g1), 12 h (g2), 24 h (g3) and 48 h (g4). the g1 group showed some cardiomyocyte with pleom ... | 2007 | 17616380 |
| structural and biological characterization of two crotamine isoforms iv-2 and iv-3 isolated from the crotalus durissus cumanensis venom. | in this work, we isolated the two new crotamine isoforms from the crotalus durissus cumanensis rattlesnake venom and its "in vitro" neurotoxic, myotoxic and lethality (dl(50)) intracerebroventricular (i.c.v.) effects were characterized. these proteins were named iv-2 and iv-3 and were purified by combination of two chromatographic steps on molecular exclusion chromatography on superdex 75 and reverse phase hplc (mu-bondapack c18). the molecular mass of the crotamine isoforms was 4905.96 da for i ... | 2007 | 17828447 |
| intraspecies differences in hemostatic venom activities of the south american rattlesnakes, crotalus durissus cumanensis, as revealed by a range of protease inhibitors. | crotalus durissus cumanensis is an endemic rattlesnake found in venezuela and colombia. in this study, a comparative analysis of hemorrhagic, coagulation and fibrino(geno)lytic activities in the presence or absence of protease inhibitors was performed with venoms of the same species crotalus durissus cumanensis, from seven geographical regions of venezuela (lagunetica, santa teresa, carrizales, guarenas, anzoátegui, margarita and maracay). lagunetica, carrizales and anzoátegui venoms induced hem ... | 2008 | 18685436 |
| biochemical and biological characterization of a pla2 from crotoxin complex of crotalus durissus cumanensis. | a new pla2 (cdcum6) from crotoxin complex of colombian crotalus durissus cumanensis rattlesnake was purified using molecular exclusion chromatography and rp-hplc. the molecular mass of cdcum6 was determined by sds-page approximately 14 kda and confirmed by maldi-tof (14321.98 da). the enzyme showed km 6.0 mm, vmax 3.44 nmol/min, optimum ph was 8.0 and temperature was between 30 and 45 degrees c, and it had a strict requirement of ca2+ for its activity. the n-terminal sequence of pla2 was slvqf e ... | 2009 | 19673100 |
| biological and biochemical characterization of two new pla2 isoforms cdc-9 and cdc-10 from crotalus durissus cumanensis snake venom. | this work reports the purification, biological characterization and amino acid sequence of two new basic pla(2) isoforms, cdc-9 and cdc-10, purified from the crotalus durissus cumanensis venom by one step analytical chromatography reverse phase hplc. the molecular masses of the pla(2) were 14,175+/-2.7 da for cdc-9 and 14,228+/-3.5 da for cdc-10 both deduced by primary structure and confirmed by maldi-tof. the isoforms presented an amino acid sequence of 122 amino acid residues, being cdc-9: slv ... | 2010 | 19747981 |
| structural and pharmacological characterization of the crotamine isoforms iii-4 (myx4_crocu) and iii-7 (myx7_crocu) isolated from the crotalus durissus cumanensis venom. | two major crotamine isoforms (iii-4 and iii-7) were obtained combining two chromatographic steps on molecular exclusion chromatography (sephadex g-75) and ion-exchange column (protein pack sp 5pw) of the rattlesnake crotalus durissus cumanensis venom. the "in vivo" myotoxic effect of the venom, its "in vitro" cytotoxicity in myoblasts and myotubes (c2c12) and the neurotoxic and edema-forming activity were characterized. the molecular masses of the crotamine isoforms were 4907.94 da (iii-4) and 4 ... | 2010 | 20206199 |
| k+(atp) channels-independent analgesic action of crotalus durissus cumanensis venom. | the effect was investigated of the k+ channel blocker, glibenclamide, on the ability of crotalus durissus cumanensis venom (cdcm) to promote peripheral antinociception. this was measured by formalin-induced nociception in male swiss mice. cdcm (200 and 300 microg/kg) produced an antinociceptive effect during phase 2 in the formalin test. the effect of cdcm (200 microg/kg) was unaffected by the atp-sensitive k+ channel blocker glibenclamide (2 mg/kg). these results suggest that cdcm is effective ... | 2010 | 20734950 |
| cloning and characterization of an antibacterial l-amino acid oxidase from crotalus durissus cumanensis venom. | an l-amino acid oxidase (laao) from crotalus durissus cumanensis venom (cdclaao) was purified to homogeneity using a combination of size-exclusion and ion exchange chromatographies. cdclaao is a monomeric protein exhibiting an apparent molecular mass of 55 kda and a calculated pi of 8. its complete 498-amino-acid sequence was deduced through cdna and protein sequencing. the enzyme oxidized l-leu with k(m) and a v(max) of 9.23 μm and 0.46 μm/min respectively, and exhibited kcat and a kcat/k(m) of ... | 2012 | 23287728 |
| toxoid preparation from the venom of crotalus durissus cumanensis (south american rattle snake). | a new technique is described for the preparation of crotalus venom toxoid. this method preserves the immunogenicity but eliminates the toxic effects. all the animals vaccinated with crotalus venom toxoid survived when they were injected with raw venom. | 2013 | 3820358 |
| antiplasmodial effect of the venom of crotalus durissus cumanensis, crotoxin complex and crotoxin b. | the antiplasmodial activity of phospholipases a(2) (pla(2)) isolated from different animals has been studied. we explored the in vitro anti plasmodium falciparum effect of a fraction containing crotoxin, crotoxin b and whole venom of the rattlesnake crotalus durissus cumanensis. fraction ii (crotoxin complex) was obtained by size exclusion chromatography, whereas crotoxin b was purified by rp-hplc. the whole venom is active against the parasite at concentrations of 0.17±0.03 μg/ml, fraction ii a ... | 2012 | 22884508 |
| the biflavonoid morelloflavone inhibits the enzymatic and biological activities of a snake venom phospholipase a2. | the biflavonoid morelloflavone has been reported as inhibitor of secretory pla2s (phospholipases a2 from human synovial and bee venom sources); however, its capacity to interact and inhibit snake venom pla2 activities has not been described. in this work we tested the inhibitory ability of morelloflavone on the enzymatic, anticoagulant, myotoxic and edema-inducing activities of a pla2 isolated from crotalus durissus cumanensis venom. the biflavonoid displayed ic50 values of 0.48 mm (95% confiden ... | 2014 | 24995575 |
| biochemical and biological characterization of two serine proteinases from colombian crotalus durissus cumanensis snake venom. | two clotting serine proteinases, named cdc si and cdc sii, were isolated and characterized for the first time from colombian crotalus durissus cumanensis snake venom. the enzymes were purified using two chromatographic steps: molecular exclusion on sephacryl s-200 and rp-hplc on c8 column. the molecular masses of the proteins, determined by maldi-tof mass spectrometry, were 28,561.4 and 28,799.2 da for cdc si and cdc sii, respectively. the aim of the present study was to evaluate enzymatic, coag ... | 2013 | 23178323 |
| inhibitory effect of pinostrobin from renealmia alpinia, on the enzymatic and biological activities of a pla2. | pinostrobin is a flavanone isolated from renealmia alpinia, a plant used in folk medicine to treat snakebites. we tested the inhibitory ability of pinostrobin on the enzymatic, anticoagulant, myotoxic and edema-inducing activities of a pla2 isolated from crotalus durissus cumanensis venom. the compound displayed ic50 values of 1.76mm and 1.85mm (95% confidence intervals: 1.34-2.18 and 1.21-2.45) on the pla2 enzymatic activity, when either aggregated or monodispersed substrates were used, respect ... | 2016 | 27109758 |
| neuromuscular effects of venoms and crotoxin-like proteins from crotalus durissus ruruima and crotalus durissus cumanensis. | a myographic study was performed to compare the neuromuscular effects of venoms and crotoxin-like proteins from crotalus durissus ruruima and crotalus durissus cumanensis in mice phrenic-diaphragm preparation. it was concluded that both venoms present neurotoxic activity as a consequence of their crotoxin content. furthermore, crotoxin from c.d. cumanensis is more potent than that from c.d. ruruima venom. at the concentration range in which both venoms express neurotoxic activity, only c.d. cuma ... | 2015 | 25598498 |
| inhibition of venom serine proteinase and metalloproteinase activities by renealmia alpinia (zingiberaceae) extracts: comparison of wild and in vitro propagated plants. | the plant renealmia alpinia has been used in folk medicine to treat snakebites in the northwest region of colombia. in addition, it has been shown to neutralize edema-forming, hemorrhagic, lethal, and defibrin(ogen)ating activities of bothrops asper venom. in this work, extracts of renealmia alpinia obtained by micropropagation (in vitro) and from specimens collected in the wild were tested and compared in their capacity to inhibit enzymatic and toxic activities of a snake venom metalloproteinas ... | 2013 | 23916793 |
| snake venomics and antivenomics of bothrops colombiensis, a medically important pitviper of the bothrops atrox-asper complex endemic to venezuela: contributing to its taxonomy and snakebite management. | the taxonomic status of the medically important pitviper of the bothrops atrox-asper complex endemic to venezuela, which has been classified as bothrops colombiensis, remains incertae cedis. to help resolving this question, the venom proteome of b. colombiensis was characterized by reverse-phase hplc fractionation followed by analysis of each chromatographic fraction by sds-page, n-terminal sequencing, maldi-tof mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of ... | 2009 | 19457355 |
| anti-platelet effect of cumanastatin 1, a disintegrin isolated from venom of south american crotalus rattlesnake. | disintegrins have been previously described in the venom of several snake families inhibiting signal transduction, cell-cell interactions, and cell-matrix interactions and may have therapeutic potential in heart attacks, thrombotic diseases, and cancers. this investigation describes the first disintegrin isolated from south american crotalus venom (venezuelan rattlesnake crotalus durissus cumanensis), which inhibits platelet adhesion to matrix proteins. c. d. cumanensis crude venom was first sep ... | 2009 | 18835011 |
| on the unsual hemorrhagic and necrotic activities caused by the rattlesnake (crotalus durissus cumanensis) in a venezuelan patient. | the hemorrhagic, necrotic and edematous effects observed in a 23-year-old patient from lagunetica, los teques, state of miranda, venezuela, that was bitten by a common venezuelan rattlesnake (crotalus durissus cumanensi), were described. the patient was treated with polyvalente serum, antibiotics and autograft. this finding allows to suggest that the poison of some venezuelan common rattlesnakes has a systemic effect on the skeletal muscle and on capillaries that generate edema, hemorragic pheno ... | 2009 | 15849951 |
| fractionation of crotalus durissus cumanensis venom by gel filtration. | | 1976 | 982480 |
| enzymatic activities and other characteristics of crotalus durissus cumanensis venom. | | 1974 | 4376285 |
| neuromuscular effect of venoms from adults and juveniles of crotalus durissus cumanensis (humboldt, 1811) from guajira, colombia. | a toxinological study was performed to compare the neuromuscular effect of venom from adult and juvenile specimens from crotalus durissus cumanensis from guajira, colombia. both venoms exhibited neurotoxic activity in chick biventer cervicis nerve-muscle preparation. in addition, venom from juveniles was faster than adults to produce a neuromuscular blockade. in the contrary to the venom from juvenile's, at high doses, adult's venom affected the ach and kcl contractures, indicating a myotoxic ef ... | 2017 | 28978413 |