| complete nucleotide sequence and genome organization of a chinese isolate of tobacco bushy top virus. | the complete nucleotide sequence of a chinese isolate of tobacco bushy top virus (tbtv), designated tbtv-ch, was determined from cdna generated from double-stranded rna extracted from diseased tobacco. the genome is 4152 nucleotides (nt) in size, contains four putative open reading frames (orfs) and untranslated regions of 10 nt and 645 nt at the 5' and 3' ends, respectively. in genome organization and in the amino acid sequence of its potential products, the rna of tbtv-ch is similar to other u ... | 2003 | 12557001 |
| [current situation and prospect of studies on genus umbravirus]. | umbraviruses are a group of imperfectly characterized plant viruses, which are distinguished from most other viruses by their genomes lack of a gene for coat protein (cp) , and as a result umbraviruses do not form conventional virus particles. umbraviruses are mechanically transmissible, and can be aphid transmitted in the persistent manner by an unrelated assistor virus, which is always a member of the family luteoviridae . in nature, each umbravirus depends for survival on one particular luteo ... | 2006 | 17302177 |
| complete nucleotide sequence and genome organization of a chinese isolate of tobacco vein distorting virus. | tobacco bushy top disease is caused by tobacco bushy top virus (tbtv, a member of the genus umbravirus) which is dependent on tobacco vein-distorting virus (tvdv) to act as a helper virus encapsidating tbtv and enabling its transmission by aphids. isometric virions from diseased tobacco plants were purified and disease symptoms were reproduced after experimental aphid transmission. the complete genome of tvdv was determined from cloned rt-pcr products derived from viral rna. it was 5,920 nucleot ... | 2010 | 20740310 |
| a novel combination of a new umbravirus, a new satellite rna and potato leafroll virus causes tobacco bushy top disease in ethiopia. | etiological studies of a recently emerged bushy top disease of tobacco in ethiopia indicated that a ~4.5-kb dsrna from infected plants represents an umbravirus, whereas a smaller band (~0.5 kb) is that of a new satellite rna. potato leafroll virus was also consistently associated with the disease. the three agents, whose experimental host ranges are restricted to members of the family solanaceae, always occurred together in field samples and are transmitted together by the aphid myzus persicae n ... | 2014 | 25119677 |
| opium poppy mosaic virus, a new umbravirus isolated from papaver somniferum in new zealand. | a novel virus, tentatively named "opium poppy mosaic virus" (opmv), was isolated from papaver somniferum (opium poppy) with leaf mosaic and mottling symptoms in auckland, new zealand, in 2006. the virus was mechanically transmitted to herbaceous plants of several species, in which it induced local and/or systemic symptoms. no virus particles were observed by electron microscopy in the diseased p. somniferum or any of the symptomatic herbaceous plants. the complete genomic sequence of 4230 nucleo ... | 2016 | 26514844 |
| phylogenetic and recombination analysis of tobacco bushy top virus in china. | during the past decade, tobacco bushy top disease, which is mainly caused by a combination of tobacco bushy top virus (tbtv) and tobacco vein-distorting virus (tvdv), underwent a sudden appearance, extreme virulence and degeneration of the epidemic in the yunnan province of china. in addition to integrative control of its aphid vector, it is of interest to examine diversity and evolution among different tbtv isolates. | 2015 | 26209518 |
| simultaneous detection of four causal agents of tobacco bushy top disease by a multiplex one-step rt-pcr. | tobacco bushy top disease is a complex disease caused by mixed infection of tobacco bushy top virus (tbtv), tobacco vein distorting virus (tvdv), satellite rna of tbtv (sat-tbtv) and tobacco vein distorting virus associate rna (tvdvarna). a one-tube multiplex reverse transcription-pcr (rt-pcr) assay was developed for simultaneous detection of the four causal agents of the disease. four pairs of specific primers based on the conserved regions of each of the four disease agents were used in the on ... | 2014 | 24833121 |