| a novel group a rotavirus g serotype: serological and genomic characterization of equine isolate fi23. | equine rotavirus fi23 was shown to be prototypic of a novel g serotype, provisionally g14, by cross-neutralization and vp7 sequence determination. although distinct, there are as few as six differing amino acid residues (92, 94, 96, 146, 147, and 221) in the vp7 antigenic regions of fi23 and g3 rotaviruses. | 1991 | 1663521 |
| serological and genomic characterization of l338, a novel equine group a rotavirus g serotype. | a group a rotavirus designated l338 was isolated from the faeces of a diarrhoeic foal and was compared to 11 standard g serotype strains of group a rotaviruses by cross-neutralization. it was clearly distinct from serotypes g1 to g11 and thus representative of a novel rotavirus g serotype tentatively designated g13. the nucleic acid sequence of the virion protein 7 (vp7) coding region was determined and the deduced amino acid sequence compared to published sequences. within vp7 regions a and b, ... | 1991 | 1851806 |
| group a rotavirus g type prevalence in two regions of hungary. | rotaviruses are a major cause of gastroenteritis in children world-wide. rotaviruses are antigenically complex, with multiple serotypes (g types). the first longitudinal study of group a rotavirus serotype (g type) distribution in hungary is reported. neutralizing monoclonal antibodies specific for g1, g2, g3, and g4 were used in an enzyme immunoassay to determine the antigenic variation of group a rotaviruses in two collections of stool specimens assembled from 1984-1992 in baranya county, sout ... | 1995 | 7503671 |
| survey of rotavirus g and p types associated with human gastroenteritis in são paulo, brazil, from 1986 to 1992. | | 1995 | 7559992 |
| typing of human group a rotavirus with alkaline phosphatase-labeled oligonucleotide probes or a monoclonal enzyme immunoassay in unfrozen stools of children with diarrhea in bangkok. | in developed countries, serotypes (or g types) have been identified in > 70% of group a rotavirus using monoclonal enzyme immunoassays (meias); however, these assays have identified < 50% of rotavirus g types from developing countries presumably because the vp7 antigens were damaged by freezing and thawing during transportation of specimens. the vp7 (g) serotypes of rotavirus in unfrozen stool collected from children with acute diarrhea in bangkok were determined using meia and compared to hybri ... | 1995 | 7714487 |
| rotavirus antigenicity is affected by the genetic context and glycosylation of vp7. | rotavirus variants resistant to neutralization were selected using monoclonal antibodies (n-mabs) raised to vp7 of rotavirus g types 2, 3, and 6. their neutralization resistance patterns and deduced vp7 amino acid sequences were obtained. variants selected by two g2-specific n-mabs from the homologous parent virus rv-5 showed single amino acid (aa) mutations in the antigenic a region. however, variants selected from reassortant virus rv-5 x sa11 (all genes from sa11 virus except that encoding vp ... | 1995 | 7747487 |
| survey of rotavirus g and p types associated with human gastroenteritis in são paulo, brazil, from 1986 to 1992. | rotavirus strains causing gastroenteritis in brazilian children were characterized by pcr-based typing assays. in addition to strains bearing the major human g and p types, large numbers of strains bearing p3 (m37-like), p6 (hcr3-like), untypeable p and g types, and complex mixtures of p and g types not previously recognized were present in the community. | 1994 | 7814514 |
| acquisition of serum isotype-specific and g type-specific antirotavirus antibodies among children in day care centers. | the acquisition of serum antirotavirus antibodies among children in day care centers was monitored through two rotavirus seasons. twenty-six children were monitored daily for diarrhea and weekly for stool rotavirus excretion through a rotavirus season of infections with serotype g1 and a successive season of infections with both g1 and g3. sera were collected before and after each rotavirus season and tested for antirotavirus iga and igg and for g type-specific blocking antibody. the prevalence ... | 1994 | 7854889 |
| [frequency of serotype g rotavirus isolated from children with diarrhea in mérida, yucatán, mexico]. | during a period of six years (1985-1990), rotavirus g serotypes were investigated in 104 fecal samples isolates according to an immune enzyme assay using specific monoclonal antibodies against serotypes 1, 2, 3, and 4 of the vp7. the serotypes were established in 65 samples (62.5%) and could not be determined in 39 samples. in the 65 classified serotypes, 7 (6.7%) were found to belong to serotype 1, 23 (22.1%) to serotype 2, 12 (11.5%) to serotype 3, and 23 (22.1%) to serotype 4. the occurrence ... | 1994 | 7973145 |
| identification of bovine and porcine rotavirus g types by pcr. | a new seminested pcr typing assay has been extended to identify the important veterinary rotavirus serotypes g5, g6, g10, and g11, as well as the rare human serotype g8. the specificity of the method was evaluated with 30 standard laboratory strains of the g1 to g6 and g8 to g11 types. rotavirus strain types g6 and g8, not previously recognized in pigs, were identified in field specimens of porcine origin. | 1994 | 8051263 |
| anti-rotavirus g type-specific and isotype-specific antibodies in children with natural rotavirus infections. | serum vp7 (g) type- and isotype-specific anti-rotavirus antibodies were assessed among children monitored longitudinally over one or two rotavirus seasons in day care centers. seventy-five pairs of blood specimens from 63 children were tested for anti-rotavirus antibodies. stool specimens were collected weekly and tested for rotavirus antigen. g typing of detected rotaviruses showed that seven outbreaks of g1 and one of g3 occurred during the two seasons. g type-specific responses to the outbrea ... | 1994 | 8158022 |
| determination of bovine rotavirus g and p serotypes by polymerase chain reaction. | among bovine rotaviruses there are two major g serotypes (g6 and g10) and three p serotypes (p1, p5, and p11, each of which is defined on the basis of the vp4 antigenic specificity of ncdv, uk and kk-3, respectively). the nucleotide sequence of a p11 gene (kk-3) was determined. the predicted kk-3 vp4 contained 772 amino-acids and showed 96% amino-acid identity with b223 vp4, an american prototype of p11 bovine rotavirus. comparative analysis of the genes determining the g and p serotypes of bovi ... | 1993 | 8232344 |
| rotavirus-specific humoral and cellular immune response after primary, symptomatic infection. | the humoral and cellular immune response after symptomatic, primary rotavirus infection was examined in 8 children < 2 years old. rotavirus-specific iga, rotavirus-specific helper t (th) cells, and neutralizing antibody responses were evaluated at the time of illness, 2-8 weeks later, and 3-5 months later. in addition, rotavirus strains associated with infection were tested by polymerase chain reaction analysis using oligonucleotide primers specific for genes 4 (p type) and 9 (g type). the absen ... | 1993 | 8388906 |
| serotype analysis of rotaviruses from different locations in malaysia. | the distribution of rotavirus g (vp7) serotypes circulating in four locations in malaysia, representing three geographical areas, was evaluated in 341 rna-positive stool specimens obtained discontinuously between 1977 and 1988 from infants and young children under the age of five years who were hospitalized with acute gastroenteritis. a total of 306 specimens (256 stool suspensions and 50 that were adapted to growth in tissue culture) that were rotavirus positive by the confirmatory enzyme-linke ... | 1993 | 8394376 |
| amino acids involved in distinguishing between monotypes of rotavirus g serotypes 2 and 4. | neutralizing monoclonal antibodies (n-mabs) to serotype g2 and g4 rotaviruses were used to study intraserotypic variation by selection and characterization of n-mab-resistant antigenic variants and reaction of n-mabs with prototype rotavirus strains. two g2-specific n-mabs reacted with g2 rotaviruses s2, ds-1, rv-5 and rv-6 but not with 1076. sequence analysis of the gene encoding vp7 of 1076 virus showed that the differences in amino acid sequence between 1076 virus and the other g2 strains at ... | 1996 | 8627227 |
| review of g and p typing results from a global collection of rotavirus strains: implications for vaccine development. | candidate rotavirus vaccines have been prepared with reassortant strains specifically to protect against the 4 major rotavirus g serotypes (g1 -4). many studies using p (vp4) genotyping methods have indicated that, worldwide, rotavirus strains of the 4 common g serotypes are each associated with 1 p genotype: gi, g3, and g4 are associated with p[8], and g2 is associated with p[4]. in contrast, g and p genotyping of rotavirus in specimens from india revealed that a high percentage of the childhoo ... | 1996 | 8752288 |
| unusual diversity of human rotavirus g and p genotypes in india. | between april and december 1993, we determined p and g genotypes of group a rotavirus strains obtained from children admitted to diarrhea treatment centers in five indian cities. from a total of 63 rotavirus-positive specimens, we identified 10 different strains with five different g genotypes and four distinct p types by using reverse transcription-pcr. the common worldwide strains g1p8, g2p4, g3p8, and g4p8 were underrepresented among indian children (33%), whereas strains of p type 6 (g1p6, g ... | 1996 | 8789033 |
| temporal distribution of rotavirus g-serotypes in the west midlands region of the united kingdom, 1983-1994. | samples of rotavirus from cases of acute gastroenteritis, occurring in the west midlands region of the united kingdom during the winter months of 1983 to 1994, were tested by enzyme-immunoassay for g-serotype (vp7). statistically significant changes in the relative prevalence of serotypes occurred each year (p-values in chi-square tests were: p < 0.05 or p < 0.01). no consistent pattern was associated with these changes. g1, g2, g3, and g4 were the most common serotypes detected throughout the s ... | 1995 | 8838827 |
| concomitant rotavirus serotypes 1 and 4 infections in a diarrhoeic child from belém, brazil. | concomitant serotypes 1 and 4 infections were detected in a 15-month old female child with community-acquired diarrhoea which lasted 7 days and coursed with moderate dehydration. the evidence for dual rotavirus infection was offered by the following findings: a) enzyme-linked immunosorbent assay (elisa) positive reactions to both 1 and 4 serotypes; and b) extra-migrating bands at electrophoresis of rna in polyacrylamide gel (page). these results suggest that children living under poor sanitation ... | 1996 | 9216104 |
| serotyping of group a rotaviruses in egyptian neonates and infants less than 1 year old with acute diarrhea. | group a human rotavirus g serotypes were detected in stool specimens from neonates and infants with and without acute diarrhea in cairo by using monoclonal antibodies in an enzyme-linked immunosorbent assay. serotypes g1 and g4 predominated in all age groups. mixed (g1 plus g4) and nontypeable specimens represented 16.1 and 38.7% of the total number serotyped, respectively. | 1997 | 9350778 |
| rotavirus g and p types circulating in brazil: characterization by rt-pcr, probe hybridization, and sequence analysis. | we used reverse transcription-polymerase chain reaction (rt-pcr) to determine the p and g genotypes of 130 culture-adapted rotavirus strains isolated from 181 fecal specimens of children under 5 years of age from 9 states and the federal district of brazil. the 4 genotypes found most commonly worldwide were also common in brazil and p[8]g1 was the most prevalent (43%), followed by p[4]g2 (12%), p[8]g3 (6%), and p[8]g4 (6%). however, unusual types p[8]g5, p[6]g2, p[9]g1, p[9]g3, and mixed infecti ... | 1996 | 9526543 |
| detection of rotavirus types g8 and g10 among brazilian children with diarrhea. | characterization of 49 rotavirus-positive stool specimens from children with diarrhea in the state of rio de janeiro, brazil, in 1996 and 1997 revealed a great diversity of rotavirus g types. conventional types g1 and g3 accounted for 27 and 12% of the infections, respectively, whereas 60% of the infections were caused by unconventional types g5 (25%), g10 (16%), and g8 (4%) and mixed g types (16%). | 1998 | 9705422 |
| rotavirus g and p types in children from belém, northern brazil, as determined by rt-pcr: occurrence of mixed p type infections. | fifty-four group a rotavirus-positive stool samples, obtained from children aged less than three years during a longitudinal (december 1982 to march 1986) study in belém, brazil, were re-examined. the samples were tested by reverse-transcription and polymerase chain reaction to determine their g-type and p-type specificity. only 17 (32%) of these rotavirus strains could be successfully g- and p-genotyped. while 10 (59%) of the 17 strains showed single g- and p-type specificity, the remaining bel ... | 1998 | 9775575 |
| rotavirus g and p types in children with acute diarrhea in blantyre, malawi, from 1997 to 1998: predominance of novel p[6]g8 strains. | one hundred rotavirus strains detected in children with acute diarrhea in blantyre, malawi, between july 1997 and january 1998 were characterized for g (vp7) and p (vp4) types by using multiplex, heminested, reverse transcription-polymerase chain reaction. a novel p[6]g8 rotavirus strain was identified in 42% of the specimens. the remaining strains comprised p[8]g3 (20%), p[6]g3 (10%), p[4]g8 (9%), p[6]g9 (3%), p[8]g4 (2%), p[6]g4 (2%), and p[4]g3 (1%). rotavirus strains with mixed g or p types ... | 1999 | 10022804 |
| rotavirus g-type restriction, persistence, and herd type specificity in swedish cattle herds. | g-typing of rotavirus strains enables the study of molecular epidemiology and gathering of information to promote disease prevention and control. rotavirus strains in fecal specimens from neonatal calves in swedish cattle herds were therefore characterized by using g1 to -4-, g6-, g8-, and g10-specific primers in reverse transcription (rt)-pcr. fecal samples were collected from one dairy herd (herd a) for 4 consecutive years and from 41 beef and dairy herds (herd b) experiencing calf diarrhea ou ... | 1999 | 10066651 |
| rotavirus gastroenteritis in finland: burden of disease and epidemiological features. | the burden of disease attributable to childhood rotavirus infection in finland was assessed from data on hospital admissions for acute gastroenteritis and from reported virological diagnoses of rotavirus from 1985 to 1995. the mean number of hospitalizations (3584 annually in children under 5 y of age) corresponded to approximately 5.6% of the birth cohort. rotavirus was estimated to be responsible for 54% of cases; accordingly, 3% of all children in finland are hospitalized for rotavirus diarrh ... | 1999 | 10088908 |
| distribution of human rotavirus g types circulating in paris, france, during the 1997-1998 epidemic: high prevalence of type g4. | group a human rotavirus g genotypes were determined by means of reverse transcription-pcr in 170 stool specimens from children with acute diarrhea admitted to a paris children's hospital during a 1-year survey (1997 to 1998). the isolates all belonged to types g1 to g4, with type g4 predominating (60%). | 1999 | 10364621 |
| comparison of enzyme immunoassay and reverse transcriptase pcr for identification of serotype g9 rotaviruses. | while only four globally important rotavirus g serotypes (1 to 4) have been documented, many studies suggest that serotype g9 viruses may be widely distributed and more important than previously recognized. we have evaluated 10 serotype g9 rotavirus-neutralizing monoclonal antibodies (mabs) directed to vp7, which bound by direct enzyme immunoassay (eia) to p1a[8], g9 rotaviruses f45, wi61, and au32, for their ability to recognize the new delhi g9 rotavirus 116e. only one mab (mab f45:1) bound to ... | 1999 | 10488175 |
| determination of bovine rotavirus g and p serotypes in italy by pcr. | determination of the g and p serotypes of group a bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-pcr typing assay. the g6 serotype was the most prevalent, accounting for viruses in 55.7% of the samples; viruses of the g10 and g8 serotypes were found in 34.9 and 4.7% of the samples, respectively. the virus in one sample (0.7%) was not classified due to concomitant ... | 1999 | 10565900 |
| characterisation of a "european-like" serotype g8 human rotavirus isolated in australia. | an atypical human rotavirus strain, dg8, was isolated from a 13-month-old child hospitalised with acute gastro-enteritis in australia. the virus could not be serotyped by enzyme immunoassay (eia) using standard reagents specific for common group a human rotavirus g serotypes. the deduced amino acid sequence of the outer capsid glycoprotein, vp7, indicated that this strain belonged to the uncommon human serotype g8. this was confirmed by eia incorporating a g8-specific neutralising monoclonal ant ... | 2000 | 10568764 |
| vp7 and vp4 genotyping of human group a rotavirus in buenos aires, argentina. | specific and sensitive tests for the detection and typing of group a rotavirus strains are needed for a more comprehensive knowledge of the epidemiology of rotaviral infection. in this study 500 stool specimens taken from 1996 to 1998 from children with acute diarrhea in buenos aires were examined. group a rotavirus was unequivocally demonstrated in 62% of the samples tested by enzyme-linked immunosorbent assay (elisa) for detection of vp6 antigen, polyacrylamide gel electrophoresis of double-st ... | 2000 | 10618096 |
| g and p genotyping of rotavirus strains circulating in france over a three-year period: detection of g9 and p[6] strains at low frequencies. the azay group. | rotavirus g and p types from 716 children with acute diarrhea in dijon from 1995 to 1998 and throughout france during the winter of 1997-1998 were analyzed by reverse transcription-pcr. p[8],g1 predominated, followed by p[8],g4, which emerged during the last winter. g9 and p[6] strains were detected at low frequencies. | 2000 | 10747170 |
| distribution of rotavirus vp4 genotypes and vp7 serotypes among nonhospitalized and hospitalized patients with gastroenteritis and patients with nosocomially acquired gastroenteritis in austria. | to assess the potential benefits of a reassortant tetravalent rotavirus vaccine, we investigated stool specimens from children in three different groups by reverse transcription-pcr (rt-pcr) for rotavirus g and p types: (i) children not hospitalized with community-acquired rotavirus-acute gastroenteritis (rv-age), (ii) children hospitalized for rv-age, and (iii) children with nosocomially acquired rv-age. from a total of 553 samples investigated, 335 were positive by enzyme-linked immunosorbent ... | 2000 | 10790103 |
| analysis of human rotavirus g serotype in bangladesh by enzyme-linked immunosorbent assay and polymerase chain reaction. | distribution of human rotavirus g serotype was investigated by enzyme-linked immunosorbent assay (elisa) and polymerase chain reaction (pcr) with faecal specimens obtained from children with diarrhoea in bangladesh. by elisa, subgroup and g serotype were determined for 59.5% and 28.6% of group a rotavirus-positive specimens respectively. however, of the 120 specimens, the g serotype of which was not determined by elisa, serotype of the 112 specimens was typed by pcr. in total, g serotype was ass ... | 1999 | 10892493 |
| rotavirus infection in children in japan. | currently, a high morbidity of rotavirus diarrhea has been seen in children in developed and developing countries. improvement of the vaccines is necessary in order to reduce the burden of diarrhea caused by rotavirus. a survey of rotavirus infection from diarrheal stool specimens in children of seven regions in japan was conducted from 1984 to 1999. the present study discusses the survey results and reviews the national and international data of more than 23 papers and congress proceedings abou ... | 2000 | 10986883 |
| vp7 and vp4 genotypes among rotavirus strains recovered from children with gastroenteritis over a 3-year period in valencia, spain. | between september 1996 and may 1999, the incidence and distribution of the main human rotavirus g genotypes (vp7 associated: g1-g4) and p genotypes (vp4 associated: p[8], p[4], p[6] and p[9]) among children with rotavirus gastroenteritis were determined using reverse transcription and polymerase chain reaction (rt-pcr)-based genotyping methods. from a total of 145 rotavirus strains examined, we identified the g type in 131 (90.3%) and the p type in 127 (87.5%) of the samples. an overall predomin ... | 2000 | 11049091 |
| [identification of rotavirus associated to serotype g2 in yucatan, mexico]. | in the present study, rotavirus g2 serotype was identified from fecal samples of children with gastroenteritis from the city of merida, yucatan, mexico. virological diagnosis of disease was performed using polycrylamide gel electrophoresis and immunoenzymatic assay. out of 149 analyzed samples 25 (16.7%) gave positive reaction to rotavirus groups a, of these 23 (92%) were identified as serotype g2, subgroup i and electrophoretic short pattern, whereas 2 (8%) were identified as subgroups ii and e ... | 2000 | 11175585 |
| rotavirus g and p genotypes in rural ghana. | an epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural upper eastern region of ghana during 1998. fifty isolates, randomly selected from 165 human group a rotavirus-positive samples, were g and p characterized by a reverse transcription (rt)-pcr assay using a seminested multiplex method. rotaviruses of the g3 genotype were found to be the predominant strain (78%), followed by g2 (14%) and g1 (2%). mixed i ... | 2001 | 11326029 |
| distribution of rotavirus g and p types in north and south indian children with acute diarrhoea in 1998-99. | vp7 and vp4 genotypes of 82 rotavirus strains from children with acute diarrhoea during november 1998-january 1999, in 4 indian towns, were determined by reverse-transcription pcr. overall, 68/82 (83%) could be vp7- and 52/82 (63%) vp4-typed. geographical differences in rotavirus circulation have implications for future vaccination strategies. | 2001 | 11706656 |
| bovine rotavirus g and p types and sequence analysis of the vp7 gene of two g8 bovine rotaviruses from japan. | a total of 1481 fecal specimens were collected from diarrheic calves under 1 month of age on 29 dairy and beef farms in 11 prefectures in japan during the period from 1987 to 2000. those calves and their dams were not vaccinated against rotavirus. one hundred and forty-two bovine rotaviruses were isolated on ma-104 cell cultures and detected by latex agglutination test. they were classified into 18 g6p[1] (11.2%), 53 g6p[5] (37.3%), 15 g6p[11] (10.6%), 12 g10p[5] (8.5%), 42 g10p[11] (29.6%) and ... | 2002 | 11750138 |
| changes in the prevalence of rotavirus g and p types in diarrheic calves from the kagoshima prefecture in japan. | g8 bovine group a rotavirus was the most predominant serotype in calf diarrheal fecal specimens examined between 1995 and 1996 in japan [vet. microbiol. 66 (1999) 301]. to date, no evidence that g8 was the most predominant in the typeable specimens has been observed outside japan. to investigate whether g8 continues to be as common as g6 and g10, the incidence of the main serotypes was determined in the same area (kagoshima prefecture) between 1997 and 1998 by reverse transcription-polymerase ch ... | 2002 | 11955784 |
| detection and characterization of rotavirus g and p types from children participating in a rotavirus vaccine trial in belém, brazil. | this study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine in belém, brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life. rotavirus electropherotypes, subgroups, g serotypes, g, [p] and [p], g genotypes were determined in 93.3%, 95.9%, 93.3%, 73.3%, 95.5% and 92.2% of isolates, respectively. serotypes g1, g2 and g4 were detected in 58.9%, 30% and 4.4% of the cases, respectively. rotavirus ... | 2002 | 11992160 |
| rotavirus g serotypes and p[],g genotypes identified in cases of reinfection among children participating in a trial with rhesus-human reassortant tetravalent vaccine (rrv-tv) in belém, brazil. | group a rotaviruses are the most important agents of severe diarrhea in children and infants worldwide. the aim of present study was to identify rotavirus g serotypes and p[],g genotypes in cases of reinfection among children who participated in a vaccine trial with the tetravalent rhesus-human reassortant rotavirus vaccine (rrv-tv 4 x 10(4) pfu/dose) in belém, brazil. from july 1990 to june 1992, 540 children received, at their first, third and fifth months of life, oral doses of either vaccine ... | 2002 | 12022436 |
| genetic variation of capsid protein vp7 in genotype g4 human rotavirus strains: simultaneous emergence and spread of different lineages in argentina. | rotavirus is the most-common cause of severe diarrhea in young children. complete rotavirus characterization includes determination of the antigenic type of the two outer capsid proteins, vp7 and vp4, designated g and p types, respectively. during a nationwide rotavirus surveillance study, genotype g4 frequency increased during the second year. to evaluate further the mechanism of emergence and the relationship among g4 strains, the genetic diversity of vp7 capsid protein in these samples was st ... | 2002 | 12037057 |
| survey of porcine rotavirus g and p genotype in poland and the united states using rt-pcr. | porcine rotaviruses are a common cause of gastroenteritis. several serotypes have been detected based on the two surface proteins vp4 (p-types) and vp7 (g-types). however, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. in this study, we describe a reverse transcriptase-polymerase chain reaction (rt-pcr) method for the typing of p and g types of rotavirus. this method allowed to ... | 2002 | 12449245 |
| genetic characterization of a novel, naturally occurring recombinant human g6p[6] rotavirus. | a binary classification system has been established for group a rotaviruses, with the viral capsid protein vp7 defining g types and vp4 defining p types. at least 15 g types and 21 p types have been isolated globally with various g and p combinations. most of the currently circulating human rotaviruses belong to g1p[8], g2p[4], g3p[8], and g4p[8]. we report a human rotavirus strain (b1711) with a novel genotypic vp7/vp4 combination of g6p[6]. this unique rotavirus was isolated from a 13-month-ol ... | 2003 | 12734253 |
| [use of pcr and nucleotide sequence analysis of the vp7 gene for detection of bovine rotaviruses and identification of their g serotypes]. | a vp7 gene fragment pcr protocol was developed to detect the bovine rotaviruses and to identify their g serotypes. the most widespread bovine rotaviruses of g serotypes (g6, g8 and g10) can be distinguished on the basis of the pcr fragment size, while other g serotypes can be differentiated through a comparative analysis of the vp7 gene fragment nuclcotide sequence. twenty-four bovine rotavirus field isolates were detected, and their g stereotypes were determined by using the method in question. ... | 2003 | 12800773 |
| viral agents of acute gastroenteritis in german children: prevalence and molecular diversity. | acute gastroenteritis is a major source of morbidity and mortality among young children in developed and developing countries. enteropathogenic viruses are regarded as particularly relevant causative agents. between february 2001 and january 2002, fecal specimens were obtained from german children admitted to hospital with acute gastroenteritis and examined for rotaviruses, noroviruses, enteric adenoviruses, and astroviruses using (rt-)pcr methods. of the 59% (129/217) samples positive for > or ... | 2003 | 12858413 |
| distribution of human rotaviruses, especially g9 strains, in japan from 1996 to 2000. | a 4-year (1996-2000) survey of rotavirus infection involving 2,218 diarrheal fecal specimens of children collected from five regions of japan was conducted. a total of 642 (28.9%) specimens were found to be rotavirus positive. a changed prevalence pattern of rotavirus g serotype was found with an increase of g9 and g2 and a decrease of g1, although g1 remained the prevailing serotype. serotype g9 was unexpectedly determined to be the prevailing serotype in sapporo (62.5%) and tokyo (52.9%) in 19 ... | 2003 | 14524620 |
| [molecular epidemiologic survey of rotaviruses from infants and children with diarrhea in shanghai]. | to investigate molecular epidemiologic features of rotaviruses circulating in shanghai, china. | 2004 | 14990097 |
| [nosocomial gastroenteritis and asymptomatic rotavirus and astrovirus infection in hospitalized children]. | nosocomial gastroenteritis is frequent in pediatric hospital wards. between 20% and 50% of gastroenteritis cases caused by rotavirus and astrovirus are of nosocomial origin. | 2004 | 15033111 |
| diversity of group a human rotavirus types circulating over a 4-year period in madrid, spain. | the incidence and distribution of human rotavirus g types among children under 5 years old with acute gastroenteritis were determined over a 4-year period (1998 to 2002) by using monoclonal antibodies and reverse transcription-pcr methods. rotavirus was detected in 1,155 (31%) of 3,760 specimens tested. rotavirus was studied in every month of the 48-month survey period. rotavirus activity occurred mainly (51%) in the typically cooler months in spain (november to february). the age distribution o ... | 2004 | 15071013 |
| epidemiology of rotavirus diarrhoea in iranian children. | human rotavirus is the most important cause of severe diarrhoea in infants and young children worldwide. we describe the aetiology of viral diarrhoea and the characteristics of rotavirus infection in shahrekord, iran. two hundred and fifty nine children <5 years old admitted to hajar hospital, 245 children with acute diarrhoea attending primary health centres in shahrekord, and 114 children hospitalised for elective surgery were selected from october 2001 to august 2002. stool samples were scree ... | 2004 | 15122809 |
| g and p genotypes of rotavirus circulating among children with diarrhea in the colombian northern coast. | a study on the prevalence of rotavirus g and p genotypes was carried out based on 253 stool specimens obtained from children living in the colombia northern coast region who were less than 3-years-old and who suffered from acute diarrhea. a previous study had detected the presence of rotavirus a in 90 (36.5%) of the 246 samples tested by enzyme immunoassay (eia), and these strains were investigated in the present study. of these, 50 strains yielded an rna electropherotype, most of which (80.0%) ... | 2004 | 15248159 |
| global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. | a safe and effective rotavirus vaccine is urgently needed, particularly in developing countries. critical to vaccine development and implementation is a knowledge base concerning the epidemiology of rotavirus g and p serotypes/genotypes throughout the world. the temporal and geographical distribution of human rotavirus g and p types was reviewed by analysing a total of 45571 strains collected globally from 124 studies reported from 52 countries on five continents published between 1989 and 2004. ... | 2005 | 15484186 |
| changing epidemiology of rotavirus g-types circulating in hong kong, china. | group a rotaviruses are the most common cause of severe diarrhoeal disease in young children worldwide. the development of a vaccine is advocated by the world health organization. obtaining local baseline information regarding rotavirus strain variation is important to ensure matching of circulating and vaccine strains. the current study was undertaken to determine the epidemiology of rotavirus g-types in hong kong in anticipation of a vaccination program. from 2001 to 2002 over a period of one ... | 2005 | 15543567 |
| determination of bovine rotavirus g genotypes in kashmir, india. | rotavirus ribonucleic acid (rna) was extracted from ten faecal samples of diarrhoeic calves positive for group a rotavirus by enzyme-linked immunosorbent assay (elisa). a portion of the extracted rna was run in polyacrylamide gel to determine the presence of rotaviral rna and the rest subjected to reverse transcriptase-polymerase chain reaction (rt-pcr) to generate the near full length vp7 gene. only six samples yielded the desired product. the amplified products were subjected to g-typing by pc ... | 2004 | 15861888 |
| changing distribution of group a rotavirus g-types and genetic analysis of g9 circulating in japan. | a total of 1,797 fecal specimens from infants and children with acute gastroenteritis in japan from july 2000 to june 2003 were tested for group a rotavirus by elisa, rt-pcr, rna-page and latex agglutination methods. of these, 439 were found to be positive for group a rotavirus and this presented 24.4%. in 2000-2001, g1 was the most prevalent (45.5%) followed by g2 (32.5%), g3 (12.3%), g9 (5.9%) and g4 (2.6%). however, g2 was found predominant with 40% in the following year (2001-2002). interest ... | 2006 | 16086098 |
| emergence of g3 and g9 rotavirus and increased incidence of mixed infections in the southern region of ireland 2001-2004. | two hundred and thirty fecal specimens were collected from children (up to 5 years of age) admitted with suspected rotaviral gastroenteritis at four irish hospitals (cork university hospital, mercy hospital, cork, waterford regional hospital, and kerry general hospital) in the southern region of ireland, between 2001 and 2004. following laboratory confirmation of the aetiological agent, the rotavirus g-type was determined in all positive samples by reverse transcriptase-polymerase chain reaction ... | 2005 | 16254970 |
| human rotavirus serotype g9, são paulo, brazil, 1996-2003. | a total of 3,101 fecal specimens were collected during an 8-year survey for rotavirus infection in são paulo, brazil. group a rotavirus was detected in 774 (25.0%) specimens. of these, 431 strains (55.7%) were analyzed for g and p types by reverse transcription-polymerase chain reaction; g1 was the predominant serotype (68.2%), followed by g9 (17.2%), g4 (6.3%), g2 (1.2%), g3 (0.7%), mixed infection (1.8%), and untypeable (4.6%). both rotavirus g and p types could be established in 332 strains ( ... | 2006 | 16707053 |
| detection of group a human rotavirus g9 genotype circulating in córdoba, argentina, as early as 1980. | the incidence of human rotavirus g types was determined over a 25-year period (1979-2003) by using reverse transcription-pcr (rt-pcr) to examine 519 stool specimens found to be positive for rotavirus by enzyme linked immunosorbent assay (elisa) or polyacrylamide gel electrophoresis (page). these stool samples were obtained from children under 3 years old who had been treated for acute diarrhea at public hospitals in córdoba, argentina. the present study describes the continued circulation of the ... | 2006 | 16789026 |
| shifts of rotavirus g and p types in nicaragua--2001-2003. | the present study reports the diversity of rotavirus strains circulating in león, nicaragua during three years. there was a shift of g and p genotypes with increment of one specific genotype during the second most important peak of diarrhea occurring in the beginning of every year. | 2006 | 17072136 |
| g and p genotyping of human rotavirus isolated in a university hospital in korea: implications for nosocomial infections. | to characterize rotavirus g and p genotypes circulating among infants and young children hospitalized with severe diarrhea in a university hospital in gyeonggi province, korea, and to examine any association of the genotypes and nosocomial infections, we genotyped 103 isolates of rotavirus by multiplex rt-pcr. in july 2001-june 2002, we found that globally common strains constituted 64.2% (g2p[4] 28.3%, g3p[8] 28.3%, g4p[8] 5.7%, and g1p[8] 1.9%), and the uncommon strain, g4p[6], constituted 26. ... | 2006 | 17179673 |
| diversity of viruses associated with acute gastroenteritis in children hospitalized with diarrhea in ho chi minh city, vietnam. | a molecular epidemiological study on common diarrheal viruses was conducted in ho chi minh city, vietnam between october 2002 and september 2003. fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. those samples were screened for groups a, b, and c rotavirus, adenovirus, genogroups i and ii norovirus (nov), sapovirus (sav), and human astrovirus (hastv) by rt-multiplex pcr, and the positive specimens were characterized further by elisa, nested pcr, or sequenc ... | 2007 | 17385670 |
| rotavirus genotypes in slovenia: unexpected detection of g8p[8] and g12p[8] genotypes. | a rotavirus surveillance study was undertaken in slovenia from december 2005 to march 2006. stool samples from 114 children hospitalized with acute viral gastroenteritis were collected from two main slovenian hospitals. these confirmed rotavirus-positive samples were selected for a rotavirus g and p genotype prevalence study. six untypable strains of genotype g were further analyzed with sequencing of the vp7, vp8*, and nsp4 genes. the findings of the study were that the g1 genotype was the most ... | 2007 | 17387749 |
| sequence analysis of the vp7 gene of human rotavirus g1 isolated in japan, china, thailand, and vietnam in the context of changing distribution of rotavirus g-types. | over the last decade, rotavirus g1 has represented the most common genotype worldwide. since 2000, the prevalence of rotavirus g1 has decreased in some countries such as japan and china. to monitor the trend of the vp7 encoding gene of rotavirus g1, we performed a sequence analysis of 74 g1 rotavirus strains isolated in japan, china, thailand, and vietnam during the period from 2002 to 2005. the phylogenetic tree showed that all of the studied g1 strains from the four countries clustered into li ... | 2007 | 17516525 |
| amino acid substitutions in the vp7 protein of human rotavirus g3 isolated in china, russia, thailand, and vietnam during 2001-2004. | the distribution of rotavirus g-types in the world appears to be changing, especially with the emergence of g3 and g9 in many countries. sequence analysis of the vp7 gene was performed on the 27 human g3 rotavirus strains isolated in china, russia, thailand, and vietnam during 2001-2004. all the strains studied were clustered into the same branch of the phylogenetic tree. the comparison of the g3 deduced amino acid sequences between the studied chinese strains and the strains circulating in chin ... | 2007 | 17705183 |
| changing pattern of rotavirus g genotype distribution in chiang mai, thailand from 2002 to 2004: decline of g9 and reemergence of g1 and g2. | group a rotaviruses are the most common cause of acute viral diarrhea in humans and animals throughout the world. previous surveillance studies of group a rotaviruses in thailand indicated that the dominant types of rotaviruses were changing from time to time. during 2000 and 2001, the g9 rotavirus emerged as the most prevalent genotype, with an exceptionally high frequency (91.6%) in chiang mai, thailand. in the year 2002-2004, group a rotavirus was detected in 98 out of 263 (37.3%) fecal speci ... | 2007 | 17854032 |
| development of a microtiter plate hybridization-based pcr-enzyme-linked immunosorbent assay for identification of clinically relevant human group a rotavirus g and p genotypes. | a microtiter plate hybridization-based pcr-enzyme-linked immunosorbent assay (pcr-elisa) has been used for the detection and identification of a variety of microorganisms. here, we report the development of a pcr-elisa for the identification of clinically relevant human rotavirus vp7 (g1 to g6, g8 to g10, and g12) and vp4 (p[4], p[6], p[8], p[9], and p[14]) genotypes. the g and p types of reference human and animal rotavirus strains for which specific probes were available were correctly identif ... | 2008 | 18057127 |
| [comparison of clinical characteristics of patients with rotavirus gastroenteritis relative to the infecting rotavirus g-p genotype.]. | group a rotavirus is a major cause of severe diarrhea in children throughout the world. for the proper management of rotavirus infections, it will be helpful to know their clinical characteristics according to the g and p genotypes of the infecting virus. | 2006 | 18156706 |
| efficacy of a pentavalent rotavirus vaccine in reducing rotavirus-associated health care utilization across three regions (11 countries). | to evaluate the effect of a human-bovine reassortant pentavalent rotavirus vaccine (prv) on health care encounters in nearly 70 000 subjects randomized in three regions - europe, the united states, and latin america/the caribbean - in the rotavirus efficacy and safety trial (rest). | 2007 | 18162243 |
| molecular characterization of bovine rotavirus strains circulating in northern italy, 2003-2005. | a total of 232 stools collected from calves with rotavirus infection in herds located in northern italy from 2003 to 2005 was investigated. determination of the rotavirus g and p types was carried out using nested rt-pcr. g6 was the most prevalent genotype, accounting for 78.5% of samples, g10 accounted for 9.9% of samples and viruses of g8 type were found in 4.7% of samples. in 3% of samples, viruses were not classified due to concomitant infection with more g type strains, whereas viruses in 3 ... | 2008 | 18191347 |
| molecular epidemiology of rotavirus diarrhea among children in saudi arabia: first detection of g9 and g12 strains. | in anticipation of rotavirus vaccine introduction in saudi arabia, this study was undertaken to determine the distribution of the g and p genotypes of rotaviruses in order to examine whether there was any emerging serotype or unusual strain circulating in children in saudi arabia. of 984 stool specimens collected between 17 april 2004 and 16 april 2005, rotavirus was detected by an enzyme-linked immunosorbent assay in 187 (19%) diarrheal children less than 5 years of age. of these, 160 (86%) wer ... | 2008 | 18234870 |
| emergence of g12 rotavirus strains in delhi, india, in 2000 to 2007. | the prospect that rotavirus diarrhea in children may soon be prevented by vaccines has placed a new priority on understanding the diversity of rotavirus strains and the mechanism by which these strains evolve over time. we have characterized a total of 465 rotavirus strains collected in north india from 2000 to 2007 for g and p types by reverse transcription-pcr and sequencing. the novel g12 rotavirus strains recently detected in other countries were first detected in india in 2001 and have emer ... | 2008 | 18272705 |
| use of polymerase chain reaction for the differentiation of group a bovine rotavirus g6, g8, and g10 genotypes in the north island of new zealand. | to determine the presence of group a rotavirus g6, g8, and g10 genotypes in calves in the north island of new zealand. | 2008 | 18836501 |
| unexpected substitution of dominant rotavirus g genotypes in french hospitalized children over five consecutive seasons. | the study was designed to evaluate the circulation of group a rotaviruses in french hospitalized children, and to detect unusual strains. this prospective study was conducted from 2001 to 2006 in children consulting for acute diarrhea at the pediatric emergency department in three french university hospitals. the rotaviruses were detected by rapid test and genotyped by rt-pcr on the basis of their outer capsid proteins vp4 (p-type) and vp7 (g-type). the stools from 757 children were analyzed. g1 ... | 2009 | 18855026 |
| diversity of human rotavirus g and p genotypes in panama, costa rica, and the dominican republic. | in this study 2,089 fecal samples from patients with gastroenteritis were analyzed from different hospitals in panama, costa rica, and the dominican republic during the period comprised between december 2002 and july 2003. one hundred samples per country from the positives to the enzyme-linked immunosorbent assay (elisa) kit were analyzed by reverse transcription-polymerase chain reaction (rt-pcr) to determine the g and p genotypes: in panama, costa rica, and dominican republic the combinations ... | 2008 | 19052306 |
| rotavirus vp7-gene selection during coinfections in caco-2 cells. | a previous rotavirus epidemiological survey in córdoba, argentina, revealed an unusually high frequency of mixed g-type infections (41.5%). the genotype distribution of those mixed infections showed that the most prevalent g-type combinations were g1+g4 (65.0%), g1+g2 (12.5%), g2+g4 (3.1%) and g1+g9 (2.5%). in the present study we analyzed the competitive growth in caco-2 cell cultures of strains from the most frequent rotavirus g-type coinfections in order to explain some aspect of the dynamic ... | 2009 | 19084615 |
| rotavirus vaccine rix4414 (rotarix): a review of its use in the prevention of rotavirus gastroenteritis. | rotavirus vaccine rix4414 is an oral vaccine composed of a monovalent, live, attenuated, human rotavirus strain of g1p[8] type. rix4414 vaccination in infants aged 6-17 weeks at enrolment provided protection against rotavirus gastroenteritis (rvge) of any severity and high-level protection against severe rvge requiring hospitalization in large, randomized clinical trials conducted in a wide range of geographic regions. protective efficacy was evident over the period (2 months) between the first ... | 2009 | 19127963 |
| genomic diversity of group a rotavirus strains in patients aged 1-36 months admitted for acute watery diarrhoea in northern india: a hospital-based study. | the diversity and capacity of human rotaviruses for rapid evolution and genetic reassortment suggests that rotavirus vaccine should be designed to provide heterotypic protection. the objective of the present study was to provide information on the circulating genotypes of rotavirus and allied baseline epidemiology in lucknow, india. in a cross-sectional study, the prevalence of rotavirus g-p types was studied in patients aged 1-36 months with acute watery diarrhoea. various sociodemographic, env ... | 2010 | 19456832 |
| a long-term survey on the distribution of the human rotavirus g type in thailand. | the distribution of the g type of human rotavirus was surveyed in thailand between july 1993 and june 2007. a significant yearly change in the distribution of the g type distribution was found. from 1993-1994 to 1998-1999, the g1 type was the most dominant. in 1999-2000, g9 began to appear at a high frequency. in 2000-2001, 2001-2002, and 2002-2003, g9 was very common. in 2003-2004, g1 became the most prevalent type again, and since then it has been detected at the highest frequency. g12 strains ... | 2010 | 19950228 |
| molecular characterization of group a human rotavirus among hospitalized children and adults in bangladesh: finding of emerging g12 strain. | between july 2004 and june 2006, a total of 1438 fecal samples were collected from hospitalized children and adults with diarrhea at an infectious disease hospital (sk hospital) in mymensingh, bangladesh. the diversity of rotavirus strains were investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. group a rotavirus was detected in 171 of 1438(11.9%) specimens: 13.3%(72/543) from children 6 months to </=15 years of age and 11.0%(99/895) from i ... | 2010 | 20046166 |
| molecular epidemiology of rotaviruses in bulgaria: annual shift of the predominant genotype. | rotavirus molecular epidemiology investigations provide important information about the incidence of rotavirus diseases and rotavirus strains in circulation in the prevaccine era. the purpose of this investigation was to study the burden of rotavirus disease, rotavirus strain diversity, and epidemiology specificities of rotavirus infections in bulgaria. a total of 3,130 stools collected between 2005 and 2008 were tested by immune enzyme tests. g-p genotype identification of rotavirus strains wer ... | 2010 | 20217165 |
| rotavirus gastroenteritis and strain diversity in saudi arabia. current status and future prospects. | to determine the epidemiological profile and strain diversity in the light of recent published studies to provide an updated comprehensive picture of the significance of the effect of this virus in saudi arabia. | 2010 | 20231932 |
| ovine rotavirus strain llr-85-based bovine rotavirus candidate vaccines: construction, characterization and immunogenicity evaluation. | group a bovine rotaviruses (brvs) are the most important cause of diarrheal diseases in neonatal calves and cause significant morbidity and mortality in the young animals, and epidemiologic surveillance of bovine rotavirus g genotypes conducted in various cattle populations throughout the world has shown that approximately 90% of the bovine rotavirus isolates belong to g6 and g10. based on the modified jennerian approach to immunization, we constructed and characterized a reassortant rotavirus s ... | 2010 | 20488633 |
| distribution of human rotavirus g and p genotypes in a hospital setting from northern india. | rotavirus gastroenteritis is a major cause of severe dehydrating diarrhea in children worldwide. rotavirus g and p genotyping is essential for epidemiological surveillance and for better formulation of candidate rotavirus vaccines. out of 862 diarrheal stool samples collected from hospitalized children aged < 2 years during february 2005 - march 2007, 318 (36.9%) were positive for rotavirus by elisa. g and p genotyping was performed on 100 randomly selected positive samples using a seminested mu ... | 2010 | 21073035 |
| a hospital based study on inter- and intragenotypic diversity of human rotavirus a vp4 and vp7 gene segments, germany. | efforts to reduce the impact of group a rotaviruses on human morbidity and mortality rely on oral immunisation with live attenuated or recombinant vaccines. a major challenge in immunisation is the vast inter- and intragenotypic diversity accomplished by circulating rotaviruses. | 2010 | 21074491 |
| recurrent rotavirus diarrhoea outbreaks in a stud farm, in italy. | a total of 47 stool samples were collected at the same stud farm from young foals with rotavirus diarrhoea and from their stud mares. illness involved foals during three consecutive winter seasons. infection in the farm appeared firstly in january-february 2008. after vanishing in the warm seasons, cases reappeared in march 2009 and 2010. determination of the rotavirus g- and p-types was carried out using nested rt-pcr in samples collected in 2009 and 2010. a total of 19 of 47 samples resulted p ... | 2010 | 21129862 |
| increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time rt-pcr assay. | the real-time taqman rt-pcr assay (pang et al., 2004) did not detect 14 clinical samples with rotavirus g2 genotype. three to five nucleotides (nt) were found to be mismatched between the published forward primer when compared to g2p[4], g2p[8], g3p[4], g9p[4], g8 and g12 sequences. an additional forward primer was designed and included in a modified assay to test the 14 clinical samples and 12 samples with known rotavirus g and p genotypes. the modified assay has improved significantly the sens ... | 2010 | 21185331 |
| instability of human rotavirus g genotypes circulating in a rural area of bangladesh. | a total of 280 fecal specimens from patients with acute gastroenteritis attending one rural hospital (dharmapasha health complex, sunamgonj) in bangladesh from august 2004 to may 2006 were tested for rotavirus by polyacrylamide gel electrophoresis (page). the diversity of rotavirus was investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. the electrophoretic patterns of dsrna of rotavirus showed 9 different migrations (6 long and 3 short) by ... | 2011 | 21240155 |
| [annual incidence of human group a rotavirus g-serotypes detected in a japanese university hospital between 2003 and 2007]. | group a rotavirus g-serotyping by polymerase chain reaction (pcr) using university hospital subject samples in september 2003 to august 2004, september 2004 to august 2005, september 2005 to august 2006, and september 2006 to august 2007 showed the most common serotypes g1 and g3, detected in 27 and 33 subjects, compared to 4 subjects in whom serotype g4 was detected. between 2003 and 2004, serotypes g1 accounted for 50% and g3 for 38%, contrasting with serotype g3 at 79% between 2004 and 2005, ... | 2011 | 21404601 |
| genotype diversity of group a rotavirus strains in children with acute diarrhea in urban burkina faso, 2008-2010. | in this study, the diversity of g and p genotypes of rotavirus strains in burkinabe children were examined. between november 2008 and february 2010, 447 stool samples were collected from children <5 years of age with acute diarrhea visiting hospital in ouagadougou. group a rotavirus was previously detected in 151/447 (33.8%) of the samples tested by an immunochromatographic test and these samples were now tested further for rotavirus g and p genotypes by rt-pcr. of these, the rotavirus type gene ... | 2011 | 21678452 |
| [Detection and genotyping of rotavirus in diarrhea infant feces in Chengdu]. | To study the genotype of rotavirus and the genetic variations of the major neutralization antigen VP4 of group A rotavirus in fecal samples from infants with diarrhea in Chengdu, Sichuan province, China. | 2011 | 21866637 |
| determination of bovine rotavirus g serotype by polymerase chain reaction. | a total of 113 diarrheic samples comprising of 68 buffalo calves and 45 cow calves were screened by rna-page for the detection of presence of rotavirus. rna-page analysis of these samples revealed 11 (9.73%) was found positive for rotavirus. out of 68 faecal samples of buffalo calves tested for viral gastroenteritis, 8 (11.76%) were found positive for rotavirus. similarly, out of 45 faecal samples of cattle calves tested for viral gastroenteritis, 3 (6.66%) was found positive for rotavirus. rota ... | 2011 | 21892766 |
| co-circulation of g1, g2 and g9 rotaviruses in hospitalized patients in bangladesh during 2006-2009. | rotavirus strain diversity in bangladesh has been explored since 1985 and as seen in other parts of the world, rotaviruses have shown tremendous strain diversity overtime. rotavirus antigen was detected in stool specimens using a solid-phase sandwich-type enzyme immunoassay. a multiplex reverse transcription polymerase chain reaction (rt-pcr) was performed for rotavirus g and p genotypes. this current study was carried out between 2006 and 2009 during which time 1,607 (23%) of 7,058 fecal specim ... | 2011 | 21860255 |
| burden of rotavirus gastroenteritis and distribution of rotavirus strains in asia: a systematic review. | background: rotavirus is the leading cause of severe diarrhea in children worldwide. we systematically reviewed the burden of rotavirus gastroenteritis (rvge) and distribution of rotavirus strains in asia. methods: we searched medline, embase and the world health organization (who) website for the term "rotavirus" and the name of each country. we included studies that were conducted in children between 2000 and 2011 and that examined the epidemiology, health and/or economic burden of rvge, and g ... | 2011 | 22212128 |
| rapid genotyping of human rotavirus using sybr green real-time reverse transcription-polymerase chain reaction with melting curve analysis. | to develop a real-time reverse transcription-polymerase chain reaction (rt-pcr) assay to genotype rotavirus (g and p) in alberta from january 2012 to june 2013. | 0 | 26568918 |
| tandem truncated rotavirus vp8* subunit protein with t cell epitope as non-replicating parenteral vaccine is highly immunogenic. | the two currently available live oral rotavirus vaccines, rotarix(®) and rotateq(®), are highly efficacious in the developed countries. however, the efficacy of such vaccines in resource deprived countries in africa and southeast asia is low. we reported previously that a bacterially-expressed rotavirus p2-p[8] δvp8* subunit vaccine candidate administered intramuscularly elicited high-titers of neutralizing antibodies in guinea pigs and mice and significantly shortened the duration of diarrhea i ... | 2015 | 26091081 |
| assessment and evaluation of an integrated hybrid anaerobic-aerobic sewage treatment system for the removal of enteric viruses. | the capability of a cost-effective and a small size decentralized pilot wastewater treatment plant (wwtp) to remove enteric viruses such as rotavirus, norovirus genogroup i (ggi), norovirus genogroup ii (ggii), hepatitis e virus (hev), and adenovirus was studied. this pilot plant is an integrated hybrid anaerobic/aerobic setup which consisted of anaerobic sludge blanket (uasb), biological aerated filter (baf), and inclined plate settler (ips). both the uasb and baf are packed with a non-woven po ... | 2017 | 28197973 |
| [surveillance for viral diarrhea in sentinel hospitals in henan province, 2013-2015]. | objective: to investigate the infection status of human rotavirus, calicivirus, astrovirus and enteric adenovirus in children aged <5 years in disease surveillance areas in henan province from 2013 to 2015. methods: a total of 880 stool samples were collected from four sentinel hospitals and group a rotavirus was detected by elisa and group a rotavirus g/p genotyping was performed with nested multiplex rt-pcr, while rotavirus (group b, c), calicivirus and astrovirus were detected by two-step mul ... | 2016 | 27765132 |