| polyamine analysis for chemotaxonomy of thermophilic eubacteria: polyamine distribution profiles within the orders aquificales, thermotogales, thermodesulfobacteriales, thermales, thermoanaerobacteriales, clostridiales and bacillales. | cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by hplc and gc analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. spermidine and a quaternary branched penta-amine, n4-bis(aminopropyl)norspermidine, were the major polyamine in thermocrinis, hydrogenobacter, hydrogenobaculum, aquifex, persephonella, sulfurihydrogenibium, hydrogenothermus, balnearium and thermovibrio, located in the order aquificales. thermo ... | 2004 | 15747232 |
| carbon isotopic fractionations associated with thermophilic bacteria thermotoga maritima and persephonella marina. | stable carbon isotopes can provide insight into carbon cycling pathways in natural environments. we examined carbon isotope fractionations associated with a hyperthermophilic fermentative bacterium, thermotoga maritima, and a thermophilic chemolithoautotrophic bacterium persephonella marina. in t. maritima, phospholipid fatty acids (plfa) are slightly enriched in 13c relative to biomass (epsilon = 0.1-0.8 per thousand). however, plfa and biomass are depleted in 13c relative to the substrate gluc ... | 2002 | 11966826 |
| sulfurihydrogenibium azorense, sp. nov., a thermophilic hydrogen-oxidizing microaerophile from terrestrial hot springs in the azores. | five hydrogen-oxidizing, thermophilic, strictly chemolithoautotrophic, microaerophilic strains, with similar (99-100%) 16s rrna gene sequences were isolated from terrestrial hot springs at furnas, são miguel island, azores, portugal. the strain, designated az-fu1t, was characterized. the motile, 0.9-2.0 microm rods were gram-negative and non-sporulating. the temperature growth range was from 50 to 73 degrees c (optimum at 68 degrees c). the strains grew fastest in 0.1% (w/v) nacl and at ph 6, al ... | 2004 | 14742456 |
| thermostable rnase p rnas lacking p18 identified in the aquificales. | the rnase p rna (rnpb) and protein (rnpa) genes were identified in the two aquificales sulfurihydrogenibium azorense and persephonella marina. in contrast, neither of the two genes has been found in the sequenced genome of their close relative, aquifex aeolicus. as in most bacteria, the rnpa genes of s. azorense and p. marina are preceded by the rpmh gene coding for ribosomal protein l34. this genetic region, including several genes up- and downstream of rpmh, is uniquely conserved among all thr ... | 2006 | 17005927 |
| persephonella hydrogeniphila sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium from a deep-sea hydrothermal vent chimney. | a novel thermophilic, hydrogen-oxidizing bacterium, designated 29w(t), was isolated from a deep-sea hydrothermal vent chimney sample collected from the suiyo seamount in the izu-bonin arc, japan, at a depth of 1385 m. the cells were cocci (0.9-1.0 microm in diameter) and straight rods (2.3-2.7 microm long) under static and agitated culture conditions, respectively. the new isolate was an obligate chemolithoautotroph growing by respiratory nitrate reduction with h2, forming n2 as a final product. ... | 2003 | 12807214 |
| sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer. | a polyphasic taxonomic study was performed on a novel facultatively anaerobic, hydrogen- or sulfur/thiosulfate-oxidizing, thermophilic chemolithoautotroph recently isolated from subsurface hot aquifer water in a japanese gold mine. the cells were straight to slightly curved rods, with a single polar flagellum. growth was observed at 40-70 degrees c (optimum 60-65 degrees c; 80 min doubling time) and at ph 6.4-8.8 (optimum ph 7.5). the isolate was unable to use complex organic compounds, carbohyd ... | 2003 | 12807207 |
| persephonella marina gen. nov., sp. nov. and persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents. | two thermophilic, strictly chemolithoautotrophic, microaerophilic, hydrogen-oxidizing members of the bacteria designated strain ex-h1t and strain ex-h2t were isolated from two separate deep-sea hydrothermal vent sites at 9 degrees n 104 degrees w in the pacific ocean and guaymas basin. the motile 2-4-microm-long rods were gram-negative and non-sporulating. the temperature range for growth was between 55 and 80 degrees c for ex- h1t (optimum at 73 degrees c) and 55-75 c for ex-h2t (optimum at 70 ... | 2002 | 12148650 |
| diversity, biological roles and biosynthetic pathways for sugar-glycerate containing compatible solutes in bacteria and archaea. | a decade ago the compatible solutes mannosylglycerate (mg) and glucosylglycerate (gg) were considered to be rare in nature. apart from two species of thermophilic bacteria, thermus thermophilus and rhodothermus marinus, and a restricted group of hyperthermophilic archaea, the thermococcales, mg had only been identified in a few red algae. glucosylglycerate was considered to be even rarer and had only been detected as an insignificant solute in two halophilic microorganisms, a cyanobacterium, as ... | 2010 | 21176052 |
| purification and characterization of the oxygen-thermostable hydrogenase from the aerobic hyperthermophilic archaeon aeropyrum camini. | aeropyrum camini that was isolated from a deep-sea hydrothermal vent chimney, possessed two hydrogenases (161 and 85 kda) in its soluble fraction. the 85-kda hydrogenase was purified to homogeneity using several chromatography columns. the specific activities of the purified hydrogenase were: 14.8 micromol methyl viologen(ox)/mg/min for hydrogen oxidation, and 14.6 micromol methyl viologen(red)/mg/min for proton reduction. the oxygen stabilities of hydrogenases that were purified from a. camini ... | 2009 | 19716518 |
| complete and draft genome sequences of six members of the aquificales. | the aquificales are widespread in marine and terrestrial hydrothermal environments. here, we report the complete and draft genome sequences of six new members of the aquificales: two marine species, persephonella marina strain ex-h1 and hydrogenivirga strain 128-5-r1 (from the east pacific rise, 9 degrees 50.3'n, 104 degrees 17.5'w, and the eastern lau spreading center, 176 degrees 11.5'w, 20 degrees 45.8's, respectively), and four terrestrial isolates, sulfurihydrogenibium azorense strain az-fu ... | 2009 | 19136599 |
| culture dependent and independent analyses of 16s rrna and atp citrate lyase genes: a comparison of microbial communities from different black smoker chimneys on the mid-atlantic ridge. | the bacterial and archaeal communities of three deep-sea hydrothermal vent systems located on the mid-atlantic ridge (mar; rainbow, logatchev and broken spur) were investigated using an integrated culture-dependent and independent approach. comparative molecular phylogenetic analyses, using the 16s rrna gene and the deduced amino acid sequences of the alpha and beta subunits of the atp citrate lyase encoding genes were carried out on natural microbial communities, on an enrichment culture obtain ... | 2008 | 18523725 |
| single-step pathway for synthesis of glucosylglycerate in persephonella marina. | a single-step pathway for the synthesis of the compatible solute glucosylglycerate (gg) is proposed based on the activity of a recombinant glucosylglycerate synthase (ggs) from persephonella marina. the corresponding gene encoded a putative glycosyltransferase that was part of an operon-like structure which also contained the genes for glucosyl-3-phosphoglycerate synthase (gpgs) and glucosyl-3-phosphoglycerate phosphatase (gpgp), the enzymes that lead to the synthesis of gg through the formation ... | 2007 | 17369297 |
| glucosylglycerate biosynthesis in the deepest lineage of the bacteria: characterization of the thermophilic proteins gpgs and gpgp from persephonella marina. | the pathway for the synthesis of glucosylglycerate (gg) in the thermophilic bacterium persephonella marina is proposed based on the activities of recombinant glucosyl-3-phosphoglycerate (gpg) synthase (gpgs) and glucosyl-3-phosphoglycerate phosphatase (gpgp). the sequences of gpgs and gpgp from the cold-adapted bacterium methanococcoides burtonii were used to identify the homologues in the genome of p. marina, which were separately cloned and overexpressed as his-tagged proteins in escherichia c ... | 2007 | 17189358 |