| [regulation of nitrogen metabolism in gram-positive bacteria]. | we searched for new members of the tnra and glnr regulons controlling assimilation of nitrogen in gram-positive bacteria. we identified the regulatory signals for these transcription factors with consensuses atgtnawwwwwwwtnacat for glnr and tgtnawwwwwwwtnaca for tnra. we described the structure and found new potential members for the tnra/glnr regulons in bacillus subtilis, b. licheniformis, geobacillus kaustophilus, oceanobacillus iheyensis, for the tnra regulon in b. halodurans and for the gln ... | 2006 | 17086994 |
| paucisalibacillus globulus gen. nov., sp. nov., a gram-positive bacterium isolated from potting soil. | a gram-positive bacterium, designated b22(t), was isolated from potting soil produced in portugal. this organism is a catalase-positive, oxidase-negative, motile, spore-forming, aerobic rod that grows optimally at 37 degrees c and ph 8.0-8.5. optimal growth occurs in media containing 1 % (w/v) nacl, although the organism can grow in 0-8 % nacl. the cell wall peptidoglycan is of the a4alpha type with a cross-linkage containing d-asp. the major respiratory quinone is menaquinone 7 and the major fa ... | 2006 | 16902018 |
| ornithinibacillus gen. nov., with the species ornithinibacillus bavariensis sp. nov. and ornithinibacillus californiensis sp. nov. | a gram-positive, aerobic, rod-shaped, motile, endospore-forming bacterium was isolated from pasteurized milk from bavaria, germany. 16s rrna gene sequence similarities indicated that strain wsbc 24001(t) was most closely related to virgibacillus species (95.3-96.1 %), oceanobacillus species (95.6-95.7 %), bacillus firmus iam 12464(t) (95.5 %) and bacillus niacini ifo 15566(t) (95.2 %). however, strain wsbc 24001(t) showed the highest level of sequence similarity to an unnamed strain, mb-9(t) (97 ... | 2006 | 16738118 |
| a thermodynamic comparison of hpr proteins from extremophilic organisms. | a thermodynamic stability study of five histidine-containing phosphocarrier protein (hpr) homologues derived from organisms inhabiting diverse environments is described. these hpr homologues are from bacillus subtilis (bs), streptococcus thermophilus (st), bacillus staerothermophilus (bst), bacillus halodurans (bh), and oceanobacillus iheyensis (oi). analyses of solvent and thermal denaturation experiments provide the cardinal thermodynamic parameters, like deltag, deltah, deltas, t(m), and delt ... | 2006 | 16566582 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading: brits culture (south africa). | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. cr(vi) reduction rate up to 8 times higher than the rate in previous cultures was achieved by the brits culture under aerobic conditions. near complete cr(vi) reduction was observed in batches under initial concentrations up to 200mg cr(vi)/l after incubati ... | 2008 | 18760438 |
| asymmetrical evolution of cytochrome bd subunits. | functionally linked genes generally evolve at similar rates and the knowledge of this particular feature of genomic evolution has been used as the basis for the phylogenetic profiling method. we illustrate here an exception to this rule in the evolution of the cytochrome bd complex. this is a two-component oxidase complex, with the subunits i and ii known to be widely present in bacteria. the subunits within the cytochrome bd complex are under the same evolutionary pressure and most likely behav ... | 2006 | 16474982 |
| virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of virgibacillus picturae to the genus oceanobacillus as oceanobacillus picturae comb. nov. with emended descriptions. | a novel virgibacillus strain, bh30097t, was isolated from a salt field near taean-gun on the yellow sea in korea. cells were gram-positive rods and bore ellipsoidal endospores in terminal positions. the optimum ph and temperature for growth of this organism were ph 7 and 25 degrees c, respectively. the main respiratory quinone was mk-7 and the major cellular fatty acids were anteiso-c(15 : 0) and iso-c(16 : 0). analysis based on 16s rrna gene sequence data revealed that the isolate formed an evo ... | 2006 | 16403894 |
| identification and characterization of salt-inducible polypeptide in paenibacillus sp., a moderately halophilic bacterium. | in response to salt, paenibacillus sp. strain xii expresses a 21.4 kda polypeptide. n-terminal sequencing and sequence homology analysis indicate homology between the n-terminal sequence of the polypeptide and a segment of the n-terminus of the spore coat associated protein cotn of oceanobacillus iheyensis, an extremely halotolerant bacteria of the deep-sea. | 2005 | 16384799 |
| d-amino acid dipeptide production utilizing d-alanine-d-alanine ligases with novel substrate specificity. | d-alanine-d-alanine ligase (ddl) is an important enzyme in the synthesis of bacterial peptidoglycan. the genes encoding ddls from escherichia coli k12 (ecddlb), oceanobacillus iheyensis jcm 11309 (oiddl), synechocystis sp. pcc 6803 (ssddl) and thermotoga maritima atcc 43589 (tmddl), the genomic dna sequences of which have been determined, were cloned and the substrate specificities of these recombinant ddls were investigated. although oiddl had a high substrate specificity for d-alanine; ecddlb, ... | 2005 | 16233841 |
| oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (oncorhynchus mykiss), and emended description of the genus oceanobacillus. | a halotolerant, obligately alkaliphilic bacterium, r-2(t), was isolated from the skin of a rainbow trout (oncorhynchus mykiss), a freshwater fish. the strain is gram-positive, ferments several carbohydrates, is rod-shaped and motile by peritrichous flagella and produces ellipsoidal spores. the isolate grows at ph 9-10 but not at ph 7-8. this micro-organism grows in 0-22% (w/v) nacl at ph 10. its major cellular fatty acids are iso-c(15:0), anteiso-c(15:0) and anteiso-c(17:0), the major isoprenoid ... | 2005 | 16014475 |
| [phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the naozhou island in zhanjiang, china]. | to understand the diversity of cultivable bacteria isolated from a sea anemone collected from the coastal water of the naozbou island in the leizhou bay on south china sea. | 2009 | 19445182 |
| halotolerant aerobic heterotrophic bacteria from the great salt plains of oklahoma. | the salt plains national wildlife refuge (spnwr) near cherokee, oklahoma, contains a barren salt flat where permian brine rises to the surface and evaporates under dry conditions to leave a crust of white salt. rainfall events dissolve the salt crust and create ephemeral streams and ponds. the rapidly changing salinity and high surface temperatures, salinity, and uv exposure make this an extreme environment. the salt plains microbial observatory (spmo) examined the soil microbial community of th ... | 2004 | 15696379 |
| identification and distribution of new insertion sequences in the genome of the extremely halotolerant and alkaliphilic oceanobacillus iheyensis hte831. | six kinds of new insertion sequences (iss), is667 to is672, a group ii intron (oi.int), and an incomplete transposon (tn852loi) were identified in the 3,630,528-bp genome of the extremely halotolerant and alkaliphilic oceanobacillus iheyensis hte831. of 19 iss identified in the hte831 genome, 7 were truncated, indicating the occurrence of internal rearrangement of the genome. all iss except is669 generated a 4- to 8-bp duplication of the target site sequence, and these iss carried 23- to 28-bp i ... | 2004 | 15500249 |
| comparative genomics of the methionine metabolism in gram-positive bacteria: a variety of regulatory systems. | regulation of the methionine biosynthesis and transport genes in bacteria is rather diverse and involves two rna-level regulatory systems and at least three dna-level systems. in particular, the methionine metabolism in gram-positive bacteria was known to be controlled by the s-box and t-box mechanisms, both acting on the level of premature termination of transcription. using comparative analysis of genes, operons and regulatory elements, we described the methionine metabolic pathway and the met ... | 2004 | 15215334 |
| horizontal transfer of drug-resistant aminoacyl-transfer-rna synthetases of anthrax and gram-positive pathogens. | the screening of new antibiotics against several bacterial strains often reveals unexpected occurrences of natural drug resistance. two examples of this involve specific inhibitors of staphylococcus aureus isoleucyl-transfer-rna synthetase 1 (ilers1) and, more recently, streptococcus pneumoniae methionyl-trna synthetase 1 (metrs1). in both cases, resistance is due to the presence of a second gene that encodes another synthetase (ilers2 or metrs2). here, we show that both s. pneumoniae metrs2 and ... | 2003 | 12792655 |
| genome sequence of oceanobacillus iheyensis isolated from the iheya ridge and its unexpected adaptive capabilities to extreme environments. | oceanobacillus iheyensis hte831 is an alkaliphilic and extremely halotolerant bacillus-related species isolated from deep-sea sediment. we present here the complete genome sequence of hte831 along with analyses of genes required for adaptation to highly alkaline and saline environments. the genome consists of 3.6 mb, encoding many proteins potentially associated with roles in regulation of intracellular osmotic pressure and ph homeostasis. the candidate genes involved in alkaliphily were determi ... | 2002 | 12235376 |
| crystal structure of a self-spliced group ii intron. | group ii introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. here we report the crystal structure of an intact, self-spliced group ii intron from oceanobacillus iheyensis at 3.1 angstrom resolution. an extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain v. the bulge of domain v adopts an unusual helical structure t ... | 2008 | 18388288 |
| cgas: a comparative genome annotation system. | recent advances in genome sequencing technology and algorithms have made it possible to determine the sequence of a whole genome quickly in a cost-effective manner. as a result, there are more than 200 completely sequenced genomes. however, annotation of a genome is still a challenging task. one of the most effective methods to annotate a newly sequenced genome is to compare it with well-annotated and closely related genomes using computational tools and databases. comparing genomes requires use ... | 2007 | 17993671 |
| salirhabdus euzebyi gen. nov., sp. nov., a gram-positive, halotolerant bacterium isolated from a sea salt evaporation pond. | a low-g+c, gram-positive bacterium, designated cvs-14(t), was recovered from a sea salt evaporation pond on the island of sal in the cape verde archipelago. this organism was catalase- and oxidase-positive. cells were motile, spore-forming aerobic rods, with an optimum growth temperature of about 35-40 degrees c and optimum ph between 7.0 and 8.5. optimal growth occurred in media containing 4-6 % (w/v) nacl, although the organism was able to grow in medium without added nacl and in medium contai ... | 2007 | 17625195 |
| [diversity of culturable bacteria associated with the sea urchin hemicentrotus pulcherrimus from naozhou island]. | to investigate the diversity of culturable bacteria isolated from the sea urchin hemicentrotus pulcherrimus collected from a tidal flat of naozhou island (20 degrees 52'n - 20 degrees 56'n 110 degrees 33'e - 110 degrees 38'e), leizhou bay, south china sea, china. | 2009 | 20112668 |
| pelagibacillus goriensis gen. nov., sp. nov., a moderately halotolerant bacterium isolated from coastal water off the east coast of korea. | a gram-positive, moderately halotolerant bacterium, designated cl-gr16(t), was isolated from coastal water off the east coast of korea. the strain was strictly aerobic, rod-shaped, motile by means of peritrichous flagella and produced ellipsoidal spores. phylogenetic analysis based on 16s rrna gene sequences revealed that the isolate represented an independent lineage within bacillus rrna group 1, showing 93.6-94.6 % similarity with respect to the genus ornithinibacillus, 94.0 % with respect to ... | 2007 | 17625193 |
| oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. | a halotolerant, facultatively alkaliphilic bacterium, designated cl-mp28t, was isolated from the surface of a sediment core sample collected at a depth of 2247 m in the ulleung basin of the east sea, korea. phylogenetic analysis of the 16s rrna gene sequence of strain cl-mp28t revealed an affiliation with the genus oceanobacillus. the sequence similarities between the isolate and type strains of members of the genus oceanobacillus were in the range 95.0-96.0 %, indicating that strain cl-mp28t re ... | 2007 | 17267988 |
| oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. | chironomids (diptera; chironomidae) are the most abundant insects in freshwater aquatic habitats. females of the genus chironomus lay egg masses containing hundreds of eggs embedded in a gelatinous matrix. a bacterial strain, designated t3944d(t), was isolated from a chironomid egg mass sampled from a waste-stabilization pond in northern israel and was found to be gram-positive, motile by peritrichous flagella, endospore-forming, halotolerant and facultatively alkaliphilic. comparative 16s rrna ... | 2007 | 17267960 |
| multidimensional analysis of the insoluble sub-proteome of oceanobacillus iheyensis hte831, an alkaliphilic and halotolerant deep-sea bacterium isolated from the iheya ridge. | we report the first proteomic analysis of the insoluble sub-proteome of the alkaliphilic and halotolerant deep-sea bacterium oceanobacillus iheyensis hte831. a multidimensional gel-based and gel-free analysis was utilised and a total of 4352 peptides were initially identified by automated ms/ms identification software. automated curation of this list using provalt reduced our peptide list to 467 uniquely identified peptides that resulted in the positive identification of 153 proteins. these iden ... | 2007 | 17146838 |
| oceanobacillus caeni sp. nov., isolated from a bacillus-dominated wastewater treatment system in korea. | a gram-positive, rod-shaped, spore-forming bacterium, strain s-11t, was isolated from the activated sludge of a bacillus-dominated wastewater treatment system in south korea and was characterized using a polyphasic approach in order to determine its taxonomic position. cells (0.5-0.6 x 2.0-2.2 microm) were motile by means of a single subpolar flagellum. they bore ellipsoidal endospores that lay in a central position in swollen sporangia. phylogenetic analysis based on 16s rrna gene sequences rev ... | 2008 | 18450698 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading. | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. complete cr(vi) reduction in aerobic cultures was achieved at a high concentration of 200 mg/l after incubation for only 65 hours. under anaerobic conditions up to 150 mg, cr(vi)/l was completely removed after incubating for 130 to 155 hours, still higher t ... | 2008 | 18701792 |
| oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in campania (italy), and emended description of oceanobacillus oncorhynchi. | a halophilic, alkalitolerant bacterium, strain 20agt, was isolated from an algal mat collected from a sulfurous spring located in santa maria incaldana (mondragone, campania region, southern italy). the isolate is gram-positive, ferments several carbohydrates and has motile, rod-shaped cells that do not sporulate. the isolate grows at ph 6.5-9.5 and in 5-20 % nacl. on the basis of 16s rrna gene sequence similarity, the strain was shown to belong to the genus oceanobacillus; strain 20agt showed 9 ... | 2006 | 16585699 |
| screening and isolation of halophilic bacteria producing extracellular hydrolyses from howz soltan lake, iran. | screening of bacteria from different areas of howz soltan playa, a hypersaline lake in the central desert zone of iran, led to the isolation of 231 moderately halophilic bacteria, which were able to grow optimally in media with 5-15% of salt, and 49 extremely halophilic microorganisms that required 20-25% of salt for optimal growth. these isolates produced a great variety of extracellular hydrolytic enzymes. a total of 195, 177, 100, 95, 92, 68, 65, 33, and 28 strains produced lipases, amylases, ... | 2009 | 19037673 |
| a novel enzymatic method for measuring mizoribine 5'-monophosphate levels in serum. | inosine 5'-monophosphate dehydrogenases (impdh) from oceanobacillus iheyensis and bacillus subtilis were biochemically characterized with the aim of establishing a mizoribine 5'-monophosphate (mzr-p) assay. mzr-p is the active metabolite of mizoribine, which has been successfully used as an immunosuppressive agent. a sensitive method for measuring the mzr-p concentration in serum, based on its ability to inhibit impdh, was developed. the method was applied to an automatic clinical analyzer, enab ... | 2008 | 19111650 |
| bacterial diversity associated with ornithogenic soil of the ross sea region, antarctica. | in the ross sea region of antarctica, ornithogenic soils form on land under adélie penguin rookeries. compared with mineral soils of the ross sea region, ornithogenic soils are generally high in microbial biomass, organic carbon, and total nitrogen and phosphorus, with high electrical conductivity and large variations in ph. the objective of this study was to assess the bacterial composition of ornithogenic soils from cape hallett and cape bird in the ross sea region using culture-independent me ... | 2009 | 19190698 |
| characterization of endogenous pyridoxal 5'-phosphate-dependent alanine racemase from bacillus pseudofirmus of4. | an open reading frame of 1100 bp in the partially sequenced genome sequence of alkaliphilic bacillus pseudofirmus of4 was identified as a putative alanine racemase gene (dadx(of4)), which was cloned and expressed in escherichia coli bl21 (de3). the encoded protein dadx(of4) was purified to homogeneity by his(6)-tag affinity column, gel filtration and ion-exchange chromatography. the amino acid sequence has highest identity with the known alanine racemase from oceanobacillus iheyensis hte831 (48% ... | 2009 | 19269582 |
| oceanobacillus soja sp. nov. isolated from soy sauce production equipment in japan. | a gram-positive, spore-forming, motile rod-shaped bacterium, designated strain y27t, was isolated from the bottom of a mold fermenter used in the process of soy sauce production. phylogenetic analysis of the 16s rrna gene sequence from this strain placed it within the genus oceanobacillus, and further sequence analysis revealed that this strain has a sequence similarity of 95.0-98.7% to other known species of oceanobacillus. the dna-dna relatedness between strain y27t and related type strains of ... | 2009 | 19590150 |
| oceanobacillus kapialis sp. nov., from fermented shrimp paste in thailand. | a gram-positive, rod-shaped, strictly aerobic, spore-forming, moderately halophilic bacterium, designated strain ssk2-2t, was isolated from fermented shrimp paste (ka-pi) produced in thailand. it contained mk-7 as the predominant menaquinone and meso-diaminopimelic acid in the cell-wall peptidoglycan. the isolate grew at 8-43 degrees c, ph 6-9 and in 0.5-24% (w/v) nacl (optimum, 6-14% nacl). the major cellular fatty acids were anteiso-c15:0 and anteiso-c17:0. phosphatidylglycerol and diphosphati ... | 2009 | 19620352 |
| computation-facilitated assignment of the function in the enolase superfamily: a regiochemically distinct galactarate dehydratase from oceanobacillus iheyensis . | the structure of an uncharacterized member of the enolase superfamily from oceanobacillus iheyensis (gi 23100298, img locus tag ob2843, pdb entry 2oqy ) was determined by the new york sgx research center for structural genomics (nysgxrc). the structure contained two mg(2+) ions located 10.4 a from one another, with one located in the canonical position in the (beta/alpha)(7)beta-barrel domain (although the ligand at the end of the fifth beta-strand is his, unprecedented in structurally character ... | 2009 | 19883118 |
| oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the iheya ridge. | an extremely halotolerant and alkaliphilic bacterium was isolated previously from deep-sea sediment collected at a depth of 1050 m on the iheya ridge. the strain, designated hte831 (jcm 11309, dsm 14371), was gram-positive, strictly aerobic, rod-shaped, motile by peritrichous flagella, and spore-forming. strain hte831 grew at salinities of 0-21% (w/v) nacl at ph 7.5 and 0-18% at ph 9.5. the optimum concentration of nacl for growth was 3% at both ph 7.5 and 9.5. the g+c content of its dna was 35. ... | 2001 | 11750818 |
| an antibiotic-resistance enzyme from a deep-sea bacterium. | we describe herein a highly proficient class a beta-lactamase oih-1 from the bacterium oceanobacillus iheyensis, whose habitat is the sediment at a depth of 1050 m in the pacific ocean. the oih-1 structure was solved by molecular replacement and refined at 1.25 a resolution. oih-1 has evolved to be an extremely halotolerant beta-lactamase capable of hydrolyzing its substrates in the presence of nacl at saturating concentration. not only is this the most highly halotolerant bacterial enzyme struc ... | 2010 | 20000704 |
| [search of streptomycin-resistant bacteria in creek water and application of maldi-tof ms to grouping of the isolated bacteria]. | a search of streptomycin-resistant bacteria was carried out using ten creek water samples collected in saga prefecture by spreading the sample water on an r2a medium containing 10 microg/ml of streptomycin. it was clarified that such streptomycin-resistant bacteria as bacillus, novosphigobium, sphingopyxis and oceanobacillus were distributed in the creek water. further, 60% of the isolates didn't form an inhibitory zone by the application of 700 microg/ml streptomycin solution in the cup method ... | 2008 | 18350749 |
| oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern. | a gram-stain-variable, motile, moderately halophilic bacterial strain, chl-21(t), was isolated from a marine solar saltern and its taxonomic position was investigated using a polyphasic approach. optimal growth of strain chl-21(t) occurred at 30-37 °c, at ph 7.0-7.5 and in the presence of 5-10 % (w/v) nacl. in phylogenetic trees based on 16s rrna gene sequences, strain chl-21(t) fell within the cluster comprising members of the genera oceanobacillus, ornithinibacillus and paucisalibacillus. stra ... | 2010 | 20061491 |
| alkaliphilic and halophilic hydrocarbon-utilizing bacteria from kuwaiti coasts of the arabian gulf. | green animate materials from the intertidal zone of the arabian gulf coast accommodated more alkaliphilic and halophilic bacteria than inanimate materials. the alkaliphilic oil-utilizing bacteria, as identified by their 16s ribonucleic acid sequences, belonged to the following genera arranged in decreasing frequences: marinobacter, micrococcus, dietzia, bacillus, oceanobacillus, and citricoccus. the halophilic oil-utilizing bacteria belonged to the genera: marinobacter, georgenia, microbacterium ... | 2007 | 17710391 |
| the tertiary structure of group ii introns: implications for biological function and evolution. | group ii introns are some of the largest ribozymes in nature, and they are a major source of information about rna assembly and tertiary structural organization. these introns are of biological significance because they are self-splicing mobile elements that have migrated into diverse genomes and played a major role in the genomic organization and metabolism of most life forms. the tertiary structure of group ii introns has been the subject of many phylogenetic, genetic, biochemical and biophysi ... | 2010 | 20446804 |
| oceanobacillus manasiensis sp. nov., a moderately halophilic bacterium isolated from the salt lakes of xinjiang, china. | three gram reaction positive, rod-shaped, moderately motile halophilic bacterial strains, designated yd3-56(t), yd16, and yh29, were isolated from the sediments of manasi and aiding salt lakes in the xinjiang region of china, respectively. the strains grew optimally at 30-37 degrees c, ph 8-11, in the presence of 5-10% (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences revealed that the strains were closely related to members of the genus oceanobacillus, exhibiting 99.1-99.2% sim ... | 2010 | 20571948 |
| isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments. | in assessing the bacterial populations present in spacecraft assembly, spacecraft test, and launch preparation facilities, extremophilic bacteria (requiring severe conditions for growth) and extremotolerant bacteria (tolerant to extreme conditions) were isolated. several cultivation approaches were employed to select for and identify bacteria that not only survive the nutrient-limiting conditions of clean room environments but can also withstand even more inhospitable environmental stresses. due ... | 2007 | 17308177 |
| bacterial community characterization and dynamics of indigo fermentation. | indigo fermentation has been traditionally performed for dyeing textiles in japan. limited information is available on the microbiota involved and the succession of the bacterial community structure during indigo reduction. we investigated the bacterial community structure associated with indigo fermentation using denaturing gradient gel electrophoresis and clone library analyses of a pcr-amplified 16s rrna gene in the early phase of fermentation carried out in our laboratory. a marked substitut ... | 2010 | 20695891 |
| oceanobacillus kimchii sp. nov. isolated from a traditional korean fermented food. | a moderate halophile, strain x50(t), was isolated from mustard kimchi, a traditional korean fermented food. the organism grew under conditions ranging from 0-15.0% (w/v) nacl (optimum: 3.0%), ph 7.0-10.0 (optimum: ph 9.0) and 15-45°c (optimum: 37°c). the morphological, physiological, and biochemical features and the 16s rrna gene sequences of strain x50(t) were characterized. colonies of the isolate were creamcolored and the cells were rod-shaped. phylogenetic analysis based on the 16s rrna gene ... | 2010 | 21221947 |
| alternative splicing by participation of the group ii intron orf in extremely halotolerant and alkaliphilic oceanobacillus iheyensis. | group ii introns inserted into genes often undergo splicing at unexpected sites, and participate in the transcription of host genes. we identified five copies of a group ii intron, designated oi.int, in the genome of an extremely halotolerant and alkaliphilic bacillus, oceanobacillus iheyensis. the oi.int4 differs from the oi.int3 at four bases. the ligated exons of the oi.int4 could not be detected by rt-pcr assays in vivo or in vitro although group ii introns can generally self-splice in vitro ... | 2011 | 21487203 |
| heterologous expression of the oceanobacillus iheyensis sigw and its anti-protein rsiw in bacillus subtilis. | pairs of the ecf sigma factor and its anti-sigma factor, sigw and rsiw, of bacillus-related species that inhabit extreme environments were heterologously expressed in b. subtilis. all the rsiws, membrane proteins, failed to fill their function of repressing cognate sigw activity, despite their close structural similarities. particularly, uncontrolled expression of oceanobacillus iheyensis oisigw due to abortive oirsiw was harmful to b. subtilis. analysis of revertants of this growth defect and s ... | 2011 | 21597191 |
| recognition of greater diversity of bacillus species and related bacteria in human faeces. | in a study looking at culturable aerobic actinobacteria associated with the human gastrointestinal tract, the vast majority of isolates obtained from dried human faeces belonged to the genus bacillus and related bacteria. a total of 124 isolates were recovered from the faeces of 10 healthy adult donors. 16s rrna gene sequence analyses showed the majority belonged to the families bacillaceae (n=81) and paenibacillaceae (n=3), with bacillus species isolated from all donors. isolates tentatively id ... | 2011 | 22041546 |
| molecular detection of oceanobacillus iheyensis in sand of brazilian beaches. | | 2008 | 19029772 |
| antifouling activities against colonizer marine bacteria of extracts from marine invertebrates collected in the colombian caribbean sea and on the brazilian coast (santa catarina). | the growth inhibition of 12 native marine bacteria isolated from aplysina sponge surfaces, the shell of a bivalve, and phytagel immersed for 48 h in sea water were used as indicator of the antifouling activity of the extracts of 39 marine organisms (octocorals, sponges, algae, and zoanthid) collected in the colombian caribbean sea and on the brazilian coast (santa catarina). gram-negative bacteria represented 75% of the isolates; identified strains belonged to oceanobacillus iheyensis, ochrobact ... | 2011 | 22191218 |
| purification, crystallization and preliminary x-ray analysis of the beta-lactamase oih-1 from oceanobacillus iheyensis. | bacterial resistance to the beta-lactam family of antibiotics is primarily the result of the deactivation of the drugs by beta-lactamase enzymes. the gene encoding the proficient beta-lactamase oih-1 from the alkaliphilic and halotolerant gram-positive bacterium oceanobacillus iheyensis has been cloned and the mature wild-type protein (comprising 274 amino-acid residues) has been expressed in escherichia coli and subsequently purified to homogeneity. oih-1 crystallized in two crystal forms both ... | 2009 | 19478436 |
| oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor. | a gram-stain-positive, neutrophilic, rod-shaped bacterium, strain a1g(t), was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. the isolate grew in the presence of 0-17.0 % (w/v) nacl and at ph 6.0-9.0; optimum growth was observed in the presence of 3.0-5.0 % (w/v) nacl and at ph 7.0. strain a1g(t) was motile, formed cream-coloured colonies, was catalase- and oxidase-positive and was able to hydrolyse aesculin, tween 40 and tween 60. che ... | 2010 | 19946056 |
| tertiary architecture of the oceanobacillus iheyensis group ii intron. | group ii introns are large ribozymes that act as self-splicing and retrotransposable rna molecules. they are of great interest because of their potential evolutionary relationship to the eukaryotic spliceosome, their continued influence on the organization of many genomes in bacteria and eukaryotes, and their potential utility as tools for gene therapy and biotechnology. one of the most interesting features of group ii introns is their relative lack of nucleobase conservation and covariation, wh ... | 2010 | 19952115 |
| comparative analysis of enzymatic stability and amino acid sequences of thermostable alkaline proteases from two haloalkaliphilic bacteria isolated from coastal region of gujarat, india. | thermostable alkaline proteases from two haloalkaliphilic bacteria, oceanobacillus iheyensis o.m.a(1)8 (eu680961) and haloalkaliphilic bacterium o.m.e(1)2 (eu680960) were studied for enzymatic properties and amino acid sequences in comparative manner. the bacteria were isolated from salt enriched soil located in okha, coastal gujarat, india. the unique aspect of the study was that alkaline protease from haloalkaliphilic bacterium o.m.a(1)8 optimally catalyzed the reaction over a wide range of te ... | 2011 | 21510974 |