| salmonella or smithella? | | 1997 | 9237744 |
| characterization of the anaerobic propionate-degrading syntrophs smithella propionica gen. nov., sp. nov. and syntrophobacter wolinii. | a strain of anaerobic, syntrophic, propionate-oxidizing bacteria, strain lypt (= ocm 661t; t = type strain), was isolated and proposed as representative of a new genus and new species, smithella propionica gen. nov., sp. nov. the strain was enriched from an anaerobic digestor and isolated. initial isolation was as a monoxenic propionate-degrading co-culture containing methanospirillum hungateii jf-1t as an h2- and formate-using partner. later, an axenic culture was obtained by using crotonate as ... | 1999 | 10319475 |
| pathway of propionate oxidation by a syntrophic culture of smithella propionica and methanospirillum hungatei. | the pathway of propionate conversion in a syntrophic coculture of smithella propionica and methanospirillum hungatei jf1 was investigated by (13)c-nmr spectroscopy. cocultures produced acetate and butyrate from propionate. [3-(13)c]propionate was converted to [2-(13)c]acetate, with no [1-(13)c]acetate formed. butyrate from [3-(13)c]propionate was labeled at the c2 and c4 positions in a ratio of about 1:1.5. double-labeled propionate (2,3-(13)c) yielded not only double-labeled acetate but also si ... | 2001 | 11282636 |
| stable-isotope probing of microorganisms thriving at thermodynamic limits: syntrophic propionate oxidation in flooded soil. | propionate is an important intermediate of the degradation of organic matter in many anoxic environments. in methanogenic environments, due to thermodynamic constraints, the oxidation of propionate requires syntrophic cooperation of propionate-fermenting proton-reducing bacteria and h(2)-consuming methanogens. we have identified here microorganisms that were active in syntrophic propionate oxidation in anoxic paddy soil by rrna-based stable-isotope probing (sip). after 7 weeks of incubation with ... | 2004 | 15466514 |
| fatty acid-oxidizing consortia along a nutrient gradient in the florida everglades. | the florida everglades is one of the largest freshwater marshes in north america and has been subject to eutrophication for decades. a gradient in p concentrations extends for several kilometers into the interior of the northern regions of the marsh, and the structure and function of soil microbial communities vary along the gradient. in this study, stable isotope probing was employed to investigate the fate of carbon from the fermentation products propionate and butyrate in soils from three sit ... | 2006 | 16597937 |
| phylogenetic and functional diversity of propionate-oxidizing bacteria in an anaerobic digester sludge. | the phylogenetic and functional diversity of syntrophic propionate-oxidizing bacteria (pob) present in an anaerobic digester was investigated by microautoradiography combined with fluorescent in situ hybridization (mar-fish) that can directly link 16s rrna phylogeny with in situ metabolic function. the syntrophic pob community in the anaerobic digester sludge consisted of at least four phylogenetic groups: syntrophobacter, uncultured short rod smithella (smithella sp. sr), uncultured long rod sm ... | 2007 | 17262205 |
| functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester. | functional bacteria and archaea community structures of a full-scale anaerobic sludge digester were investigated by using a full-cycle 16s rrna approach followed by microautoradiography (mar)-fluorescent in situ hybridization (fish) technique and micromanipulation. fish analysis with a comprehensive set of 16s and 23s rrna-targeted oligonucleotide probes based on 16s rrna clone libraries revealed that the gram-positive bacteria represented by probe hgc69a-hybridized actinobacteria (8.5+/-1.4% of ... | 2007 | 17291558 |
| carbon and sulfur cycling by microbial communities in a gypsum-treated oil sands tailings pond. | oil sands tailings ponds receive and store the solid and liquid waste from bitumen extraction and are managed to promote solids densification and water recycling. the ponds are highly stratified due to increasing solids content as a function of depth but can be impacted by tailings addition and removal and by convection due to microbial gas production. we characterized the microbial communities in relation to microbial activities as a function of depth in an active tailings pond routinely treate ... | 2010 | 21128661 |
| the quantitative significance of syntrophaceae and syntrophic partnerships in methanogenic degradation of crude oil alkanes. | libraries of 16s rrna genes cloned from methanogenic oil degrading microcosms amended with north sea crude oil and inoculated with estuarine sediment indicated that bacteria from the genera smithella (deltaproteobacteria, syntrophaceace) and marinobacter sp. (gammaproteobacteria) were enriched during degradation. growth yields and doubling times (36 days for both smithella and marinobacter) were determined using qpcr and quantitative data on alkanes, which were the predominant hydrocarbons degra ... | 2011 | 21914097 |