| traditional herbal remedies used for the treatment of urinary schistosomiasis in zimbabwe. | a total of 286 traditional healers, 85% of them registered with the zimbabwe national traditional healers' association (zinatha), in five administrative provinces of zimbabwe, were interviewed to assess their knowledge about the signs and symptoms of urinary schistosomiasis. information on the names of plants used to treat schistosoma haematobium infections was solicited. haematuria was mentioned by 99% of the traditional healers to be the most obvious sign of s. haematobium infection. general b ... | 1994 | 8072305 |
| crystal structure of pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose. | the crystal structure of the man/glc-specific seed lectin from pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. the carbohydrate binding site contains a classic man/glc type specificity loop. its metal binding loop on the other hand is of the long type, different from what is observed in other man/glc-specific legume lectins. glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin a and lentil lectin. ... | 2003 | 12595543 |
| structural basis of oligomannose recognition by the pterocarpus angolensis seed lectin. | the crystal structure of a man/glc-specific lectin from the seeds of the bloodwood tree (pterocarpus angolensis), a leguminous plant from central africa, has been determined in complex with mannose and five manno-oligosaccharides. the lectin contains a classical mannose-specificity loop, but its metal-binding loop resembles that of lectins of unrelated specificity from ulex europaeus and maackia amurensis. as a consequence, the interactions with mannose in the primary binding site are conserved, ... | 2004 | 14729339 |
| studies on antibacterial, antioxidant and fibroblast growth stimulation of wound healing remedies from south africa. | aqueous and methanol extracts of urtica urens, capparis tomentosa, dicoma anomala, leonotis leonorus, xysmalobium undulatum, helichrysum foetidum, pterocarpus angolensis, terminalia sericea and gunnera perpensa, plants documented as being used for topical wound healing in the literature, were tested for antibacterial activity against staphylococcus aureus, streptococcus pyogenes, escherichia coli and pseudomonas aeruginosa. methanol and water extracts of two of these plants, terminalia sericea a ... | 2004 | 15507359 |
| crystallization and crystal manipulation of the pterocarpus angolensis seed lectin. | the man/glc-specific legume lectin from the seeds of the african bloodwood tree (pterocarpus angolensis) was crystallized in the presence of the disaccharide ligand man(alpha1-3)manme. small crystals initially appeared from a preliminary screen, but proved difficult to reproduce. the initial crystals were used to prepare microseeds, leading to a reproducible crystallization protocol. all attempts to obtain crystals directly of the ligand-free protein or of other carbohydrate complexes failed. ho ... | 2005 | 15930620 |
| structural basis for the recognition of complex-type biantennary oligosaccharides by pterocarpus angolensis lectin. | the crystal structure of pterocarpus angolensis lectin is determined in its ligand-free state, in complex with the fucosylated biantennary complex type decasaccharide na2f, and in complex with a series of smaller oligosaccharide constituents of na2f. these results together with thermodynamic binding data indicate that the complete oligosaccharide binding site of the lectin consists of five subsites allowing the specific recognition of the pentasaccharide glcnac beta(1-2)man alpha(1-3)[glcnac bet ... | 2006 | 16704415 |
| how a plant lectin recognizes high mannose oligosaccharides. | the crystal structure of pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (man) oligosaccharides ranging from man-5 to man-9. despite that several of the nine man residues of man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence manalpha(1-2)manalpha(1-6)[manalpha(1-3)]manalpha(1-. isothermal titration calorimetry titration experiments using man-5, man ... | 2007 | 17556509 |
| prevalence, pathogenesis, antibiotic susceptibility profiles, and in-vitro activity of selected medicinal plants against aeromonas isolates from stool samples of patients in the venda region of south africa. | the prevalence, pathogenic indices, such as haemolytic and haemagglutinating activities, antibiograms, and in-vitro activities of local medicinal plants against aeromonas isolates in vhembe district of limpopo province, south africa, were studied using standard microbiological methods. in total, 309 diarrhoeic stool samples were collected from patients attending five health centres in the region during december 2004-may 2005. aeromonas species were identified using the api 20e system. the haemag ... | 2007 | 18402186 |
| in-vitro cytotoxicity and antimicrobial activities, against clinical isolates of campylobacter species and entamoeba histolytica, of local medicinal plants from the venda region, in south africa. | in the quest for alternative treatments against campylobacter jejuni and entamoeba histolytica, which are both aetiological agents of diarrhoea world-wide, the in-vitro activities against the two pathogens of extracts of 18 south african medicinal plants have recently been assessed. forty extracts from the 18 plant species were prepared and tested against 110 clinical isolates of campylobacter spp. in addition, extracts from eight of the plant species were tested against a standard strain (hm-1: ... | 2009 | 19208300 |
| stability, subunit interactions and carbohydrate-binding of the seed lectin from pterocarpus angolensis. | from 1 kg of defatted pterocarpus angolensis (mukwa tree) seed meal, 21.6 grams of an alpha,d-mannose/glucose-specific lectin can be purified on mannose-sepharose. relative affinities for several (oligo)saccharides and glycoproteins were studied by haemagglutination-inhibition. gel filtration shows that the lectin exists as a dimer above ph 5 and as a monomer below ph 3.5. this is confirmed by studies on the release of lectin subunits that were adsorbed from solution to lectin monomers immobiliz ... | 2009 | 19508209 |
| crude extracts of, and purified compounds from, pterocarpus angolensis, and the essential oil of lippia javanica: their in-vitro cytotoxicities and activities against selected bacteria and entamoeba histolytica. | in a recent study, various extracts of pterocarpus angolensis were prepared and tested against bacteria. the acetone extract was found to be the most active against all the bacteria investigated, with minimum inhibitory concentrations varying from 0.0156 mg/ml against staphylococcus aureus to 2 mg/ml against enterobacter cloacae. seven pure compounds were subsequently isolated from the ethanol extract of p. angolensis. using several chromatographic and spectroscopic methods, the structures of fi ... | 2009 | 19583913 |
| botryosphaeriaceae associated with pterocarpus angolensis (kiaat) in south africa. | there have been several recent reports of pterocarpus angolensis (kiaat) trees dying in south africa, zambia and zimbabwe, where this tree is used in traditional medicine and is a valuable source of timber for woodcarving and furniture. a survey of material from diseased p. angolensis trees in south africa yielded isolates of the botryosphaeriaceae, an important fungal family known to cause a number of tree diseases. the aim of this study was to identify these botryosphaeriaceae and to determine ... | 2010 | 21186325 |
| a lectin from platypodium elegans with unusual specificity and affinity for asymmetric complex n-glycans. | lectin activity with specificity for mannose and glucose has been detected in the seed of platypodium elegans, a legume plant from the dalbergieae tribe. the gene of platypodium elegans lectin a has been cloned, and the resulting 261-amino acid protein belongs to the legume lectin family with similarity with pterocarpus angolensis agglutinin from the same tribe. the recombinant lectin has been expressed in escherichia coli and refolded from inclusion bodies. analysis of specificity by glycan arr ... | 2012 | 22692206 |
| anti-inflammatory and mutagenic evaluation of medicinal plants used by venda people against venereal and related diseases. | inflammation is a major risk factor for various human diseases including venereal diseases, often resulting in treatment complications. plants have been traditionally used for treatment of many different diseases and have been successfully proven to be an alternative source in treatment of infectious diseases. | 2013 | 23276783 |
| ethno-veterinary control of bovine dermatophilosis and ticks in zhombe, njelele and shamrock resettlement in zimbabwe. | a structured questionnaire survey was conducted to determine the ethno-veterinary practices and other control methods used by smallholder farmers for the management of bovine dermatophilosis and ticks. a total of 153 farmers were interviewed from njelele, zhombe communal and shamrock resettlement areas. crop production contributed most to livelihoods (83.2 %) while livestock contributed 9.0 %. over 90 % of the respondents had attended school up to primary level, with 11.4 % undergoing animal hea ... | 2013 | 23054800 |