| characterization of condensed tannins purified from legume forages: chromophore production, protein precipitation, and inhibitory effects on cellulose digestion. | to identify simple screening tools for selecting condensed tannin (ct)-containing forages as candidate sources for further study, ct were isolated from nine legumes, and their molecular weights (mw), chromophore production, capacity to precipitate bovine serum albumin (bsa) and fraction 1 protein (rubisco) isolated from alfalfa, and inhibition of filter paper digestion were compared. sources were as follows: leaves of sericea lespedeza (lespedeza cuneata dum.-cours.), crown vetch (coronilla vari ... | 2005 | 16132212 |
| a high-throughput method for the quantification of proanthocyanidins in forage crops and its application in assessing variation in condensed tannin content in breeding programmes for lotus corniculatus and lotus uliginosus. | lotus corniculatus and lotus uliginosus are agronomically important forage crops used in ruminant livestock production. the condensed tannin (ct) content, dry matter (dm) production, and persistence of these species are key characteristics of interest for future exploitation of these crops. here we present field data on 19 varieties of l. corniculatus, 2 varieties of l. uliginosus and, additionally, a glasshouse experiment using 6 varieties of l. corniculatus and 2 varieties of l. uliginosus. cu ... | 2008 | 18193833 |
| interference of condensed tannin in lignin analyses of dry bean and forage crops. | legumes with high concentrations of condensed tannin (pinto bean [phaseolus vulgaris l.], sainfoin [onobrychis viciifolia scop.], and big trefoil [lotus uliginosus hoff.]), were compared to a selection of forages, with low or zero condensed tannin (smooth bromegrass [ bromus inermis leyss], lotus japonicus [regel] k. larsen, and alfalfa [medicago sativa l.]), using four methods to estimate fiber or lignin. protocols were validated by using semipurified condensed tannin polymers in adulteration a ... | 2008 | 18841900 |
| ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range. | eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ... | 2009 | 19477097 |
| phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes. | lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ... | 2011 | 22092879 |
| phytochemical profile and antimicrobial properties of lotus spp. (fabaceae). | the phytochemical profile and antimicrobial activity of cultivar (cv.) extracts of lotus uliginosus (cvs. trojan and serrano), l. tenuis (cv. larrañaga) and l. corniculatus (cv. são gabriel) were investigated. the phytochemical analysis revealed tannins, coumarins and flavonoids in all extracts, with variations among cultivars, showing genotypic variability. by high performance liquid chromatographic method, the cvs. larrañaga and são gabriel showed the highest percentage of catechin and epicate ... | 2014 | 25014916 |