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characterisation of wild legume nodulating bacteria (lnb) in the infra-arid zone of tunisia.we report on the isolation and the characterization of nitrogen-fixing root nodule bacteria isolated from natural legumes in a region of south tunisia corresponding to the infra-arid climatic zone. a collection of 60 new bacterial root nodule isolates were obtained from 19 legume species belonging to the genera acacia, anthyllis, argyrolobium, astragalus, calycotome, coronilla, ebenus, genista, hedysarum, hippocrepis, lathyrus, lotus, medicago, ononis. the isolates were characterised by (1) comp ...200415214644
multilocus sequence analysis of root nodule isolates from lotus arabicus (senegal), lotus creticus, argyrolobium uniflorum and medicago sativa (tunisia) and description of ensifer numidicus sp. nov. and ensifer garamanticus sp. nov.nine isolates from argyrolobium uniflorum, lotus creticus , medicago sativa (tunisia) and lotus arabicus (senegal) were analysed by multilocus sequence analysis (mlsa) of five housekeeping genes (reca, atpd, glna, glta and thrc), the 16s rrna gene and the nodulation gene noda. analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, a, b and c, ...201019656922
symbiotic diversity of ensifer meliloti strains recovered from various legume species in tunisia.ensifer meliloti (formerly sinorhizobium meliloti) was first considered as a specific microsymbiont of medicago, melilotus and trigonella. however, strains of e. meliloti were recovered from root nodules of various legume species and their symbiotic status still remains unclear. here, we further investigate the specificity of these strains. a collection of 47 e. meliloti strains isolated in tunisia from root nodules of medicago truncatula, medicago sativa, medicago ciliaris, medicago laciniata, ...200919665858
isoflavone determination in spontaneous legumes identified by dna barcodes.isoflavones have been associated with several health protective effects. in this work spontaneous legume plants were screened as putative sources of dietary isoflavones. a molecular identification of the collected species was performed throughout dna barcoding using its, rbcl, rpoc1 and matk sequences. the use of a multi-locus barcoding system complemented with basic morphological information allowed the unequivocal identification at the species level of 90% of the samples. the determination of ...201223442684
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