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quorum sensing-controlled evr regulates a conserved cryptic pigment biosynthetic cluster and a novel phenomycin-like locus in the plant pathogen, pectobacterium carotovorum.pectobacterium carotovorum scri193 is a phytopathogenic gram-negative bacterium. in this study, we have identified a novel cryptic pigment biosynthetic locus in p. carotovorum scri193 which we have called the pectobacterium orange pigment (pop) cluster. the pop cluster is flanked by two trna genes and contains genes that encode non-ribosomal peptide synthases and polyketide synthase and produces a negatively charged polar orange pigment. orange pigment production is activated when an adjacent tr ...201020192973
when genome-based approach meets the "old but good": revealing genes involved in the antibacterial activity of pseudomonas sp. p482 against soft rot pathogens.dickeya solani and pectobacterium carotovorum subsp. brasiliense are recently established species of bacterial plant pathogens causing black leg and soft rot of many vegetables and ornamental plants. pseudomonas sp. strain p482 inhibits the growth of these pathogens, a desired trait considering the limited measures to combat these diseases. in this study, we determined the genetic background of the antibacterial activity of p482, and established the phylogenetic position of this strain. pseudomo ...201627303376
genetic diversity of pectobacterium carotovorum subsp. brasiliensis isolated in korea.the plant pathogenic bacterial genus pectobacteirum consists of heterogeneous strains. the p. carotovorum species is a complex strain showing divergent characteristics, and a new subspecies named p. carotovorum subsp. brasiliensis has been identified recently. in this paper, we re-identified the p. carotovorum subsp. brasiliensis isolates from those classified under the subspecies carotovorum and newly isolated p. carotovorum subsp. brasiliensis strains. all isolates were able to produce plant c ...201425288994
draft genome sequences for canadian isolates of pectobacterium carotovorum subsp. brasiliense with weak virulence on potato.pectobacterium carotovurum subsp. brasiliense causes soft rot and blackleg diseases on potato. here, we report the draft genome sequences of three weakly virulent p. carotovurum subsp. brasiliense strains isolated in canada. analysis of these genome sequences will help to pinpoint differences in virulence among p. carotovurum subsp. brasiliense strains from tropical/subtropical and temperate regions, such as canada and united states. a small number of key factors for adaptation to this bacterium ...201525858837
rna-seq profiling reveals defense responses in a tolerant potato cultivar to stem infection by pectobacterium carotovorum ssp. brasiliense.pectobacterium carotovorum subsp. brasiliense is a member of the soft rot enterobacteriaceae (sre) family that causes tuber soft rot and blackleg diseases of stems in potato plants. currently, there are no effective chemical strategies for the control of members of the sre. thus, an understanding of the inducible defense responses in stems of potato plants is important, particularly during colonization of the vascular system. here, time-course rna-sequencing analysis was used to compare expresse ...201628066465
draft genome of a south african strain of pectobacterium carotovorum subsp. brasiliense.the draft genome of pectobacterium carotovorum subsp. brasiliense (pcb) which causes blackleg of potato was submitted to the ncbi and released with reference number nz_lgrf00000000.1. the estimated genome size based on the draft genome assembly is 4,820,279bp from 33 contigs ranging in length from 444 to 1,660,019 nucleotides. the genome annotation showed 4250 putative genes, 4114 cds and 43 pseudo-genes. three complete rrna gene species were detected: nine 5s, one 16s and one 23s. other partial ...201627939377
genome-wide identification of potato long intergenic noncoding rnas responsive to pectobacterium carotovorum subspecies brasiliense infection.long noncoding rnas (lncrnas) represent a class of rna molecules that are implicated in regulation of gene expression in both mammals and plants. while much progress has been made in determining the biological functions of lncrnas in mammals, the functional roles of lncrnas in plants are still poorly understood. specifically, the roles of long intergenic nocoding rnas (lincrnas) in plant defence responses are yet to be fully explored.201627515663
draft genome sequence of a virulent pectobacterium carotovorum subsp. brasiliense isolate causing soft rot of cucumber.pectobacterium carotovorum subsp. brasiliense causes soft rot and blackleg diseases on potatoes, ornamentals, and other crops of economic importance. here, we report a draft genome sequence of a highly virulent p. carotovorum subsp. brasiliense strain, pcbhpi01, isolated from a cucumber in south africa.201626744374
the use of fluorescent reporter protein tagging to study the interaction between root-knot nematodes and soft rot enterobacteriaceae.the study of plant parasitic nematodes such as meloidogyne spp. and their interactions with phytopathogenic bacteria remains underexplored. one of the challenges towards establishing such interactions is the dependence on symptom development as a measure of interaction. in this study, mcherry was employed as a reporter protein to investigate the interaction between the soft rot enterobacteriaceae (sre) pectobacterium carotovorum subsp. brasiliensis (pcb) and root-knot nematode (m. incognita). pe ...201323347004
[identification of soft rot pathogens on chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing].this study aimed to identify soft rot pathogens of chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing.201526939453
colonization patterns of an mcherry-tagged pectobacterium carotovorum subsp. brasiliense strain in potato plants.pectobacterium carotovorum subsp. brasiliense is a newly identified member of the potato soft rot enterobacteriaceae. the pathogenesis of this pathogen is still poorly understood. in this study, an mcherry-p. carotovorum subsp. brasiliense-tagged strain was generated to study p. carotovorum subsp. brasiliense-potato plant interactions. prior to use, the tagged strain was evaluated for in vitro growth, plasmid stability, and virulence on potato tubers and shown to be similar to the wild type. fou ...201323758294
taxonomic relatedness between pectobacterium carotovorum subsp. carotovorum, pectobacterium carotovorum subsp. odoriferum and pectobacterium carotovorum subsp. brasiliense subsp. nov.pectobacterium carotovorum is a heterogeneous species consisting of two named subspecies, p. carotovorum subsp. carotovorum and p. carotovorum subsp. odoriferum. a third subspecies, p. carotovorum subsp. brasiliense, was previously proposed. the study aimed to confirm the subspecies status and validate the proposed name of p. carotovorum subsp. brasiliense using a novel and standard microbial taxonomy.201222747943
plant growth and resistance promoted by streptomyces spp. in tomato.plant growth promoting rhizobacteria (pgpr) represent an alternative to improve plant growth and yield as well as to act as agents of biocontrol. this study characterized isolates of streptomyces spp. (stm) as pgpr, determined the antagonism of these isolates against pectobacterium carotovorum subsp. brasiliensis (pcb), evaluated the ability of stm on promoting growth and modulating the defense-related metabolism of tomato plants, and the potential of stm isolates on reducing soft rot disease in ...201728756346
influence of the ferric uptake regulator (fur) protein on pathogenicity in pectobacterium carotovorum subsp. brasiliense.iron is an important nutrient for the survival and growth of many organisms. in order to survive, iron uptake from the environment must be strictly regulated and maintained to avoid iron toxicity. the ferric uptake regulator protein (fur) regulates genes involved in iron homeostasis in many bacteria, including phytopathogens. however, to date, the role played by fur in the biology of pectobacterium carotovorum subsp. brasiliense (pcb1692), an important pathogen of potatoes, has not yet been stud ...201728545065
transcriptome-wide identification and characterization of potato circular rnas in response to pectobacterium carotovorum subspecies brasiliense infection.little information about the roles of circular rnas (circrnas) during potato-pectobacterium carotovorum subsp. brasiliense (pcb) interaction is currently available. in this study, we conducted the systematic identification of circrnas from time series samples of potato cultivars valor (susceptible) and bp1 (disease tolerant) infected by pcb. a total of 2098 circrnas were detected and about half (931, 44.38%) were intergenic circrnas. and differential expression analysis detected 429 significantl ...201729280973
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