| complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1. | pineapple mealybug wilt-associated virus-1 (pmwav-1; family closteroviridae, genus ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. in this study, the complete genome of pmwav-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family. the genome encoded seven open reading frames (orfs) and was unusual for an ampelovirus due to the lack of an intergenic region between the rdrp and p6 orfs, an orf encoding a relatively ... | 2008 | 18283409 |
| genome organization and phylogenetic relationship of pineapple mealybug wilt associated virus-3 with family closteroviridae members. | the nucleotide sequence of pineapple mealybug wilt associated virus-3 (pmwav-3) (closteroviridae: ampelovirus), spanning seven open reading frames (orfs) and the untranslatable region of the 3' end was determined. based on the amino acid identities with orthologous orfs of pmwav-1 (54%-73%) and pmwav-2 (13%-35%), we propose pmwav-3 is a new species in the pmwav complex. pmwav-3 lacks an intergenic region between orf1b and orf2, encodes a relatively small, 28.8 kda, coat protein, and lacks a coat ... | 2009 | 19225875 |
| genomic analysis of grapevine leafroll associated virus-5 and related viruses. | the grapevine leafroll-associated viruses (glravs) (closteroviridae) represent an emerging threat to world grape production. one group of glravs within the genus ampelovirus, the glrav-4-like viruses (glrav-4lvs), contains a fragmented collection of seven viruses only two of which (glrav-pr and glracv) are fully sequenced. here in reporting the sequence of glrav-5, a member of glrav-4lvs, we identify genomic elements common to the glrav-4lv group. exclusive properties include a highly conserved ... | 2011 | 21893115 |
| analysis of pineapple mealybug wilt associated virus -1 and -2 for potential rna silencing suppressors and pathogenicity factors. | higher plants use rna silencing to defend against viral infections. as a counter defense, plant viruses have evolved proteins that suppress rna silencing. mealybug wilt of pineapple (mwp), an important disease of pineapple, has been associated with at least three distinct viruses, pineapple mealybug wilt associated virus -1, -2, and -3 (pmwav-1, -2, and -3). selected open reading frames (orfs) of pmwav-1 and pmwav-2 were screened for their local and systemic suppressor activities in agrobacteriu ... | 2015 | 25751306 |
| complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus ampelovirus. | a new grapevine leafroll-associated virus isolate (glrav-pr) from greek grapevines was recently reported. this virus, along with the genetically related glrav-4, -5, -6 and -9, form a separate diverse lineage within the genus ampelovirus. in this paper, the complete nucleotide sequence of glrav-pr was determined, making it the first fully sequenced virus of this lineage. its genome is 13,696 nt long and contains seven open reading frames, which potentially encode a 253-kda polyprotein containing ... | 2009 | 19115034 |
| development of an immunomagnetic capture-reverse transcriptase-pcr assay for three pineapple ampeloviruses. | a semi-automated, immunomagnetic capture-reverse transcription pcr (imc-rt-pcr) assay for the detection of three pineapple-infecting ampeloviruses, pineapple mealybug wilt-associated virus-1, -2 and -3, is described. the assay was equivalent in sensitivity but more rapid than conventional immunocapture rt-pcr. the assay can be used either as a one- or two-step rt-pcr and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf ti ... | 2009 | 18996414 |
| closterovirus infection and mealybug exposure are necessary for the development of mealybug wilt of pineapple disease. | abstract the roles of pineapple mealybug wilt-associated viruses (pmwavs) and mealybug (dysmicoccus spp.) feeding in the etiology of mealybug wilt of pineapple (mwp) were evaluated. container-grown pineapple (ananas comosus) plants from five commercially grown hawaiian proprietary selections and a field study utilizing a randomized complete block design were used to test four treatments for induction of mwp: pmwav-1-free and pmwav-1-infected plants maintained mealybug-free, and pmwav-1-free and ... | 2002 | 18944017 |