identification of new isolates of turnip mosaic virus that cluster with less common viral strains.turnip mosaic virus (tumv) was found infecting cultivated brassicas and wild and cultivated ornamental brassicaceae plants in different regions of spain. five new tumv isolates, originating from different host plant species (brassica cretica, brassica juncea, brassica napus, eruca vesicaria subsp. sativa and sisymbrium orientale), have been identified. the nucleotide sequences of the coat protein (cp) genes of the five isolates were determined. phylogenetic analysis of the cp sequences showed th ...200717347771
characterization and prediction by near-infrared reflectance of mineral composition of rocket (eruca vesicaria subsp. sativa and eruca vesicaria subsp. vesicaria).background: minerals are essential for human nutrition and must be obtained from our diet. crucifer vegetables are a good source of these nutrients. our objectives were to determine the genetic variability for mineral content and to evaluate the use of near-infrared reflectance spectroscopy (nirs) for prediction of ashes and minerals among and within the rocket species eruca vesicaria subsp. sativa and vesicaria. the minerals studied were iron (fe), copper (cu), sodium (na), potassium (k), calci ...201122052744
effect of climate change on alternaria leaf spot of rocket salad and black spot of basil under controlled environment.plant responses to elevated co2 and temperature have been much studied in recent years, but effects of climate change on pathological responses are still largely unknown. the pathosystems rocket (eruca vesicaria subsp. sativa)--alternaria leaf spot (alternaria japonica) and basil (ocimum basilicum)--black spot (colletotrichum gloeosporioides) were chosen as models to assess the potential impact of increased co2 and temperature on disease incidence and severity under controlled environment. potte ...201223878979
flagella mediate attachment of enterotoxigenic escherichia coli to fresh salad leaves.enterotoxigenic escherichia coli (etec) causes child and travelers' diarrhea and is presumed to be water- and food-borne. sporadic outbreaks were traced to consumption of contaminated fresh produce, particularly salad leaves as lettuce and parsley. importantly, the mechanism by which etec binds salad leaves is not known. in this study we investigated the ability of clinical etec isolates to adhere to eruca vesicaria (commonly known as rocket). towards this end we inoculated pieces of cut e. vesi ...201123761239
interaction of enteroaggregative escherichia coli with salad leaves.enteroaggregative escherichia coli (eaec) are important human pathogens. however, their environmental reservoir is unknown. as fresh salad leaves are increasingly recognized as an important environmental vector for human pathogens, we investigated leaf attachment capability of eaec strains. we found that binding of clinical eaec isolates to leaves from eruca vesicaria (commonly known as rocket or arugula) can be divided into high, moderate and low adherent phenotypes. using the prototype eaec st ...200923765852
effectiveness of washing procedures in reducing salmonella enterica and listeria monocytogenes on a raw leafy green vegetable (eruca vesicaria).vegetables are an important source of nutrients, but they can host a large microbial population, particularly bacteria. foodborne pathogens can contaminate raw vegetables at any stage of their production process with a potential for human infection. appropriate washing can mitigate the risk of foodborne illness consequent to vegetable consumption by reducing pathogen levels, but few data are available to assess the efficacy of different practices. in the present work, six different washing metho ...201627812356
chemical composition and biological activities of eruca vesicaria subsp. longirostris essential oils.context to date, there are no reports to validate the tunisian traditional and folklore claims of eruca vesicaria (l) cav. subsp. longirostris (brassicaceae) for the treatment of disease. objective investigation of the chemical composition antimicrobial and antioxidant activity of essential oils from eruca longirostris leaves, stems, roots and fruits. materials and methods the essential oils of e. longirostris from leaves, stems, roots and fruits were obtained after 4 h of hydrodistillation. che ...201626983675
cloning and characterization of the dehydration-responsive element-binding protein 2a gene in eruca vesicaria subsp sativa.eruca vesicaria subsp sativa is one of the most tolerant cruciferae species to drought, and dehydration-responsive element-binding protein 2a (dreb2a) is involved in responses to salinity, heat, and particularly drought. in this study, a gene encoding evdreb2a was cloned and characterized in e. vesicaria subsp sativa. the full-length evdreb2a cdna sequence contained a 388-bp 5'-untranslated region (utr), a 348-bp 3'-utr, and a 1002-bp open reading frame that encoded 334 amino acid residues. the ...201627525923
cryopreservation of lipid-rich seeds: effect of moisture content and cooling rate on germination.the effect of fast and slow cooling on germination of seeds from two brassicaceae species (eruca vesicaria (l.) cav., brassica napus l. var. oleifera (moench) dc cv. bingo) and cypselas from three compositae species (onopordum nervosum boiss., onopordum acanthium l., helianthus annuus l. cv. viky) at different moisture contents was studied. seed lipid content (dry weight basis) ranged from 15% (o. nervosum) to 41% (h. annuus). for each species, seeds with four moisture contents were cryopreserve ...201311788852
cloning and characterization of the nicotianamine synthase gene in eruca vesicaria subsp sativa.nicotianamine (na) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. na synthesis is catalyzed by the enzyme nicotianamine synthase (nas). eruca vesicaria subsp sativa is highly tolerant to ni, pb, and zn. in this study, a gene encoding evnas was cloned and characterized in e. vesicaria subsp sativa. the full-length evnas cdna sequence contained a ...201526782459
a comparative study of flavonoid compounds, vitamin c, and antioxidant properties of baby leaf brassicaceae species.a comparative study of antioxidant compounds, flavonoids and vitamin c, and also antioxidant activity was carried out in four species of brassicaceae vegetables used for salads: watercress ( nasturtium officinale r. br.), mizuna [ brassica rapa l. subsp. nipposinica (l.h. bailey) haneltand], wild rocket [ diplotaxis tenuifolia (l.) dc.], and salad rocket [ eruca vesicaria (l.) cav.]. the characterization of individual phenolic compounds by hplc-dad-ms/ms-esi in watercress and mizuna completes th ...200818321050
ethnomedicinal plant use value in the lakki marwat district of pakistan.medicinal plants are regional treasures for the treatment of many ailments. the present research investigated and documented knowledge of indigenous commonly used medicinal plants, including traditional names, preparations and uses, in the lakki marwat district of pakistan. the information gathered was statistically analyzed using the icf method to establish baseline data for more comprehensive investigations of bioactive compounds of indigenous medicinal plants.201425448507
in vivo biological activity of rocket extracts (eruca vesicaria subsp. sativa (miller) thell) and sulforaphane.eruca is thought to be an excellent source of antioxidants like phenolic compounds, carotenoids, glucosinolates and their degradation products, such as isothiocyanates. sulforaphane is one of the most potent indirect antioxidants of eruca isolated until the date. in this work we investigate: (i) the safety and dna protective activity of eruca extracts and sulforaphane (under and without oxidative stress) in drosophila melanogaster; and (ii) the influence on d. melanogaster life span treated with ...201222369966
volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration.the nature and kinetics of reactions in dry seeds determines how long the seeds survive. we used gas chromatography to assay volatile organic compounds (vocs) emitted from seeds of three unrelated species as a means to non-invasively probe chemical changes during very dry, dry, and humid storage (seeds were dried to 5.5, 33, and 75% relative humidity at room temperature). vocs emitted from seeds stored in humid conditions reflected fermentation-type reactions, with methanol and ethanol being pre ...201626956506
identification and quantification of glucosinolate and flavonol compounds in rocket salad (eruca sativa, eruca vesicaria and diplotaxis tenuifolia) by lc-ms: highlighting the potential for improving nutritional value of rocket crops.liquid chromatography mass spectrometry (lc-ms) was used to obtain glucosinolate and flavonol content for 35 rocket accessions and commercial varieties. 13 glucosinolates and 11 flavonol compounds were identified. semi-quantitative methods were used to estimate concentrations of both groups of compounds. minor glucosinolate composition was found to be different between accessions; concentrations varied significantly. flavonols showed differentiation between genera, with diplotaxis accumulating q ...201525442630
effect of water content and temperature on seed longevity of seven brassicaceae species after 5 years of storage.maximising seed longevity is crucial for genetic resource preservation and longevity of orthodox seeds is determined by environmental conditions (water content and temperature). the effect of water content (down to 0.01 g·h₂o·g(-1) ) on seed viability was studied at different temperatures for a 5-year storage period in taxonomically related species. seeds of seven brassicaceae species (brassica repanda, eruca vesicaria, malcolmia littorea, moricandia arvensis, rorippa nasturtium-aquaticum, sinap ...201524804799
cytotoxic and genotoxic effects of metal(oid)s bioactivated in rocket leaves (eruca vesicaria subsp. sativa miller).rocket is an important source of essential elements. however, it may also accumulate toxic elements such as metal(oids). the objectives of the present work were (i) to study the uptake of arsenic, lead, cadmium and zinc in rocket grown in contaminated soils, (ii) to establish the genotoxic and cytotoxic activities of this vegetable material, and (iii) to study the modulator role of the glucosinolate and metal contents in the genotoxic/cytotoxic activities. lead, cadmium and zinc leaf concentrati ...201324161580
effect of compost and manure amendments on zinc soil speciation, plant content, and translocation in an artificially contaminated soil.the addition of organic matter in soil can modify the bioavailability of heavy metals. a greenhouse pot experiment was carried out using an edible plant species eruca vesicaria l. cavalieri grown on an artificially contaminated soil with zn (665 mg kg(-1)). in this study, the effect of compost at 20 t ha(-1) (c20) and at 60 t ha(-1) (c60), manure at 10 t ha(-1) (m10) and at 30 t ha(-1) (m30), and chemical fertilizers (npk) on zn fate in a soil-plant system was evaluated. at the end of the experi ...201323292226
rocket salad (diplotaxis and eruca spp.) sensory analysis and relation with glucosinolate and phenolic content.salad crops of the brassicaceae family, such as diplotaxis tenuifolia and eruca vesicaria, commonly referred to as 'rocket salads', have attracted considerable interest as culinary vegetables because of their strong flavour and their content of putative health-promoting compounds. among such compounds, glucosinolates and phenolics are well-known phytochemicals with an important role also in determining the characteristic flavour of these species. in this study, to identify potentially high-value ...201121725983
identification of species in tribe brassiceae by dot-blot hybridization using species-specific its1 probes.simple, reliable methods for identification of species are required for management of many species and lines in a plant gene bank. species-specific probes were designed from published sequences of the its1 region in rdna of 16 species in brassica and its related genera, and used as probes for dot-blot hybridization with plant genomic dna. all the probes detected species-specific signals at dot-blots of genomic dnas of the 16 species in brassica, diplotaxis, eruca, and raphanus. signals of the br ...201020683723
zinc distribution and speciation within rocket plants (eruca vesicaria l. cavalieri) grown on a polluted soil amended with compost as determined by xrf microtomography and micro-xanes.zinc distribution and speciation within different organs (root, petiole, and leaf) of the edible plant eruca vesicaria l. cavalieri were determined using synchrotron microbeam x-ray techniques (xrf microtomography and mu-xanes) for plants grown in polluted soil with or without compost amendment. data on soil derived from different extraction procedures and using mu-xanes analyses on rhizospheric soil indicated that compost amendment did not significantly influence the zn speciation and availabil ...200818410113
identification of new flavonoid glycosides and flavonoid profiles to characterize rocket leafy salads (eruca vesicaria and diplotaxis tenuifolia)."rocket" is a collective name used to term some species within the eruca and diplotaxis genera, whose leaves are characterized by a more or less pungent taste. different approaches have been carried out to differentiate both genera that have similar leaf morphologies. following our research in flavonoid profiling of the brassicaceae family using high-performance liquid chromatography/ultraviolet-diode array detection/electrospray ionization mass spectroemtry, we have investigated eruca vesicaria ...200717300153
biofumigant compounds released by field pennycress (thlaspi arvense) seedmeal.defatted field pennycress (thlaspi arvense l.) seedmeal was found to completely inhibit seedling germination/emergence when added to a sandy loam soil containing wheat (triticum aestivum l.) and arugula [eruca vesicaria (l.) cav. subsp. sativa (mill.) thell.] seeds at levels of 1.0% w/w or higher. covering the pots with petri dishes containing the soil-seedmeal mixture decreased germination of both species at the lowest application rate (0.5% w/w), suggesting that the some of the phytotoxins wer ...200515839488
direct antioxidant activity of purified glucoerucin, the dietary secondary metabolite contained in rocket (eruca sativa mill.) seeds and sprouts.rocket (eruca sativa mill. or eruca vesicaria l.) is widely distributed all over the world and is usually consumed fresh (leafs or sprouts) for its typical spicy taste. nevertheless, it is mentioned in traditional pharmacopoeia and ancient literature for several therapeutic properties, and it does contain a number of health promoting agents including carotenoids, vitamin c, fibers, flavonoids, and glucosinolates (gls). the latter phytochemicals have recently gained attention as being the precurs ...200515796582
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