Publications

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imp-gmp 5'-nucleotidase in reptiles: occurrence in a turtle, a tortoise and three species of snakes.imp-hydrolyzing activity, which is reactive with goose anti-pig lung imp-gmp 5'-nucleotidase (c-n-ii: ec.3.1.3.5) serum, was detected in extracts from liver, heart, kidney, spleen, stomach, skeletal muscle and lung from several species of reptiles: the values found in liver (u/mg protein) of one animal were: 4.5 for an ammono-ureotelic turtle (trionyx sinensis japonicus); 3.7 for an ureo-uricotelic tortoise (testudo elegans); 13-23 for three species of uricotelic snakes: elaphe quadrivirgata, el ...200312892757
occurrence and molecular characterization of cryptosporidium spp. in mammals and reptiles at the lisbon zoo.the presence of cryptosporidium parasites in mammals and reptiles kept at the lisbon zoo was investigated. a total of 274 stool samples were collected from 100 mammals and 29 reptiles. the species and genotype of the isolates identified by light microscopy were determined by nested pcr and sequence analysis of a fragment of the small subunit rrna gene. cryptosporidium oocysts were found in one black wildebeest (connochaetes gnou), one prairie bison (bison bison bison) and in one indian star tort ...200515986253
experimental transmission and induction of ranaviral disease in western ornate box turtles (terrapene ornata ornata) and red-eared sliders (trachemys scripta elegans).an experimental transmission study was designed to determine whether a causal relationship exists between a ranavirus (bstrv) isolated from a burmese star tortoise that died and the lesions observed in that tortoise. a pilot study was performed with 3 box turtles (terrapene ornata ornata) and 3 red-eared sliders (ress; trachemys scripta elegans) to assess their suitability in a larger study. based on the outcome of this study, ress were selected, and 2 groups of 4 ress received either an oral (p ...200717491069
ranavirus infection of free-ranging and captive box turtles and tortoises in the united states.iridoviruses of the genus ranavirus are well known for causing mass mortality events of fish and amphibians with sporadic reports of infection in reptiles. this article describes five instances of ranavirus infection in chelonians between 2003 and 2005 in georgia, florida, new york, and pennsylvania, usa. affected species included captive burmese star tortoises (geochelone platynota), a free-ranging gopher tortoise (gopherus polyphemus), free-ranging eastern box turtles (terrapene carolina carol ...200818957641
development and use of an indirect enzyme-linked immunosorbent assay for detection of iridovirus exposure in gopher tortoises (gopherus polyphemus) and eastern box turtles (terrapene carolina carolina).iridoviruses, pathogens typically associated with fish and amphibians, have recently been shown to cause acute respiratory disease in chelonians including box turtles, red-eared sliders, gopher tortoises, and burmese star tortoises. case reports of natural infections in several chelonian species in the united states have been reported, however the prevalence remains unknown in susceptible populations of free-ranging chelonians. to determine the prevalence of iridovirus exposure in free-ranging g ...201019931321
sulawesi tortoise adenovirus-1 in two impressed tortoises (manouria impressa) and a burmese star tortoise (geochelone platynota).sulawesi tortoise adenovirus-1 (stadv-1) is a newly discovered virus infecting endangered and threatened tortoises. it was initially described from a confiscated group of 105 sulawesi tortoises (indotestudo forsteni) obtained by the turtle survival alliance and distributed to five sites with available veterinary care across the united states. in a 3-yr period from the initial outbreak, one multi-species collection that rehabilitated and housed adenovirus-infected sulawesi tortoises experienced d ...201223082513
comparison of fresh and frozen fecal samples for detection of enteric salmonella from captive indian star tortoises (geochelone elegans).the use of frozen fecal samples in enrichment media to detect salmonella spp. strains was evaluated in indian star tortoises (geochelone elegans) to determine the utility of this test method for field collection. fresh fecal samples were collected from 10 captive adult indian star tortoises. each sample was split, with one portion in enrichment media at room temperature for 1 or 2 days before submission to a reference laboratory for standard enteric culture. the other was placed in the same enri ...201526056867
comparison of chemistry analytes between 2 portable, commercially available analyzers and a conventional laboratory analyzer in reptiles.advantages of handheld and small bench-top biochemical analyzers include requirements for smaller sample volume and practicality for use in the field or in practices, but little has been published on the performance of these instruments compared with standard reference methods in analysis of reptilian blood.201021198734
occurrence of camallanus trispinosus in a captive indian star tortoise (geochelone elegans).camallanoids are spirurid round worms known to occur in stomach and intestine of lower vertebrate animals such as fishes and reptiles. this paper records the occurrence of camallanus trispinosus in a captive indian star tortoise of guindy snake park, chennai, india for the first time during necropsy and identified on the basis of morphology of male and female worms, including eggs.201525698874
soft plastron, soft carapace with skeletal abnormality in juvenile tortoises. histopathology and isolation of a novel picornavirus from testudo graeca and geochelone elegans.a disease is described in juvenile tortoises (testudo graeca and geochelone elegans) consisting mainly of a soft carapace, soft plastron and deformed skeleton. the aim of this study was to determine histopathological lesions and the biological properties of the isolated viruses.201425323213
cryptosporidiosis caused by two distinct species in russian tortoises and a pancake tortoise.cryptosporidiosis in squamates is well documented, but there is very limited information available on cryptosporidiosis in testudines. we describe three cases of cryptosporidiosis in tortoises with associated pathology. two russian tortoises (agrionemys [testudo] horsfieldii) and a pancake tortoise (malacochersus tornieri), all from separate collections, were found dead. at necropsy, two had histological evidence of intestinal cryptosporidiosis and one had gastric cryptosporidiosis. consensus cr ...201020189312
influence of environmental humidity and dietary protein on pyramidal growth of carapaces in african spurred tortoises (geochelone sulcata).the carapaces of captive-raised tortoises (terrestrial chelonians of the zoological family testudinidae, often develop pyramidal-shaped osseous growth centrally within the horny plates. with very few exceptions (e.g. geochelone elegans, psammobates sp.), this conical growth pattern is considered to be pathologic. this very common defect is believed to be an important indicator of the quality of captive tortoise management. this study was designed to examine the effect of dietary protein level an ...200314511150
detection of mycoplasma spp., herpesviruses, topiviruses, and ferlaviruses in samples from chelonians in europe.we tested samples from 1,015 chelonians in europe for mycoplasma spp., herpesviruses, ranaviruses, picornaviruses, and ferlaviruses by pcr. mycoplasma spp. were detected in 42.1% and herpesviruses were detected in 8.0% of tested chelonians. differentiation of the herpesviruses revealed that 46.9% of the detected chelonian viruses were testudinid herpesvirus 1 (tehv-1) and 54.3% were tehv-3, including co-detections of tehv-1 and -3 in 3 tortoises. tehv-4 was detected in a leopard tortoise ( stigm ...201728754074
cultivation of multiple genera of hydrogenotrophic methanogens from different environmental niches.six genera of hydrogenotrophic methanogens, namely methanobrevibacter, methanobacterium, methanocorpusculum, methanothermobacter, methanoculleus, and methanospirillum were cultivated from diverse environmental niches like rumen, feces, gut, and sediments using by medium. we also report a putative novel genus and two novel species of methanogens isolated from termite, indian star tortoise, and green iguana.201829432849
single-incision, prefemoral bilateral oophorosalpingectomy without coelioscopy in an indian star tortoise (geochelone elegans) with follicular stasis.a female indian star tortoise (geochelone elegans) was evaluated for anorexia and suspected coelomic masses. clinical examination indicated follicular stasis. the tortoise was anesthetized and placed in dorsal left lateral recumbency for a right prefemoral approach. the ovaries and oviducts were exposed through the right prefemoral incision. the ovarian vasculature, the mesovarium, the oviduct, mesosalpinx and associated vasculature were ligated and transected. bilateral oophorosalpingectomy was ...201728781342
histochemicals mapping of beta-galactosidase in the cerebellum, medulla oblongata & spinal cord of the turtle testudo elegans. 19806777291
the effect of steroids on the serum protein fractions of the tortoise testudo elegans schoepff. 19684097810
bimodality of laying-hatching times in testudo elegans schoepff (chelonia). 196414238186
curious creatures: a multi-taxa investigation of responses to novelty in a zoo environment.the personality trait of curiosity has been shown to increase welfare in humans. if this positive welfare effect is also true for non-humans, animals with high levels of curiosity may be able to cope better with stressful situations than their conspecifics. before discoveries can be made regarding the effect of curiosity on an animal's ability to cope in their environment, a way of measuring curiosity across species in different environments must be created to standardise testing. to determine t ...201829568703
hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in sri lanka.this study was conducted to determine hematological reference ranges and morphological characteristics blood cells in healthy captive sri lankan fresh water turtle and tortoise species.201931583221
diagnosis and therapeutic management of gastrointestinal parasitism among the captive population of indian star tortoise (geochelone elegans schoepff, 1795) at zoological garden, thiruvananthapuram, kerala, india.indian star tortoise (geochelone elegans schoepff, 1795) is a terrestrial herbivorous reptile that is classified under the vulnerable species of iucn-red list. the present communication reports the successful management of gastrointestinal parasitism due to co-infection with oxyurids and ascarid nematodes in the captive population of indian star tortoise maintained at zoological garden, thiruvananthapuram, kerala, india. the diseased tortoise exhibited clinical signs like diarrhea, anorexia, and ...202032508422
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