| characterization of cypovirus isolates from tropical and temperate indian saturniidae silkworms. | cytoplasmic polyhedrosis virus (cypovirus, cpv) isolates from larvae of saturniidae silkworms antheraea mylitta, antheraea proylei, and antheraea assamensis were obtained and characterized. scanning electron microscopic (sem) studies revealed the presence of hexahedral (rhombic dodecahedron) polyhedral bodies of various size (1-4 microns) in individual hosts while transmission electron microscopic (tem) studies of isolated virions showed their uniform (icosahedral) shape and size (approximately ... | 2000 | 11332278 |
| molecular cloning and characterization of antheraea mylitta cytoplasmic polyhedrosis virus genome segment 9. | genome segment 9 of the 11-segment rna genomes of three cytoplasmic polyhedrosis virus (cpv) isolates from antheraea mylitta (amcpv), antheraea assamensis (aacpv) and antheraea proylei (apcpv) were converted to cdna, cloned and sequenced. in each case, this genome segment consists of 1473 nucleotides with one long orf of 1035 bp and encodes a protein of 345 amino acids, termed nsp38, with a molecular mass of 38 kda. secondary structure prediction showed the presence of nine alpha-helices in the ... | 2002 | 12029164 |
| molecular cloning and characterization of antheraea mylitta cytoplasmic polyhedrosis virus polyhedrin gene and its variant forms. | the segments 10 (s10) of the 11 double stranded rna genomes from antheraea mylitta cytoplasmic polyhedrosis virus (amcpv) encoding a novel polyhedrin polypeptide was converted to cdna, cloned, and sequenced. three cdna clones consisting of 1502 (amcpv10-1), 1120 (amcpv10-2), and 1415 (amcpv10-3) nucleotides encoding polyhedrin of 254, 339, and 319 amino acids with molecular masses of 29, 39, and 37 kda, respectively, were obtained, and verified by northern analysis. these clones showed 70-94% se ... | 2005 | 15907799 |
| molecular cloning, sequence analysis and expression of genome segment 7 (s7) of antheraea mylitta cypovirus (amcpv) that encodes a viral structural protein. | the genome segment 7 (s7) of the 11 double stranded rna genomes from antheraea mylitta cypovirus (amcpv) was converted to cdna, cloned and sequenced. the nucleotide sequence showed that segment 7 consisted of 1789 nucleotides with an orf of 530 amino acids and could encode a protein of approximately 61 kda, termed p61. the 5' terminal sequence, agtaat and the 3' terminal sequence, agagc of the plus strand was found to be the same as genome segment 10 of amcpv encoding polyhedrin. no sequence sim ... | 2007 | 17253125 |
| genome segment 6 of antheraea mylitta cypovirus encodes a structural protein with atpase activity. | the genome segment 6 (s6) of the 11 double stranded rna genomes from antheraea mylitta cypovirus was converted into cdna, cloned and sequenced. s6 consisted of 1944 nucleotides with an orf of 607 amino acids and could encode a protein of 68 kda, termed p68. motif scan and molecular docking analysis of p68 showed the presence of two cystathionine beta synthase (cbs) domains and atp binding sites. the orf of amcpv s6 was expressed in e. coli as his-tag fusion protein and polyclonal antibody was ra ... | 2008 | 18486179 |
| molecular characterization of genome segment 2 encoding rna dependent rna polymerase of antheraea mylitta cytoplasmic polyhedrosis virus. | genome segment 2 (s2) from antheraea mylitta cypovirus (amcpv) was converted into cdna, cloned and sequenced. s2 consisted of 3798 nucleotides with a long orf encoding a 1116 amino acid long protein (123 kda). blast and phylogenetic analysis showed 29% sequence identity and close relatedness of amcpv s2 with rna dependent rna polymerase (rdrp) of other insect cypoviruses, suggesting a common origin of all insect cypoviruses. the orf of s2 was expressed as 123 kda soluble his-tagged fusion protei ... | 2010 | 20488502 |
| molecular characterization of genome segments 1 and 3 encoding two capsid proteins of antheraea mylitta cytoplasmic polyhedrosis virus. | antheraea mylitta cytoplasmic polyhedrosis virus (amcpv), a cypovirus of reoviridae family, infects indian non-mulberry silkworm, antheraea mylitta, and contains 11 segmented double stranded rna (s1-s11) in its genome. some of its genome segments (s2 and s6-s11) have been previously characterized but genome segments encoding viral capsid have not been characterized. | 2010 | 20684765 |