| burkholderia anthina sp. nov. and burkholderia pyrrocinia, two additional burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. | nineteen burkholderia cepacia-like isolates of human and environmental origin could not be assigned to one of the seven currently established genomovars using recently developed molecular diagnostic tools for b. cepacia complex bacteria. various genotypic and phenotypic characteristics were examined. the results of this polyphasic study allowed classification of the 19 isolates as an eighth b. cepacia complex genomovar (burkholderia anthina sp. nov.) and to design tools for its identification in ... | 2002 | 12052570 |
| distribution and genomic location of active insertion sequences in the burkholderia cepacia complex. | this study aimed firstly to establish the distribution and copy number within the burkholderia cepacia complex of three insertion sequences (is402, is407 and is1416) that possess the ability to activate transcription and hence influence gene expression. a second aim was to map the genomic insertion sites of one of the active insertion sequences (is407) to establish putative links between insertion site and downstream gene activation. the resulting data revealed that all three insertion sequences ... | 2006 | 16388024 |
| matrix-assisted laser desorption ionisation-time-of of-flight mass spectrometry of intact cells allows rapid identification of burkholderia cepacia complex. | the present study examined the potential of intact-cell matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry (maldi-tof ms) for a rapid identification of burkholderia cepacia complex (bcc) bacteria using an applied biosystems 4700 proteomics analyser. two software packages were used to analyse mass profiles based on densitometric curves and peak positions. the 75 strains examined, represented the nine established bcc species and some commonly misidentified species, closel ... | 2008 | 18627778 |
| the structure of the o-specific polysaccharide from the lipopolysaccharide of burkholderia anthina. | the pathogenic mechanisms of gram-negative infection in cystic fibrosis are only just beginning to be explored at molecular level. several virulence factors have been defined, one of the most important is the lipopolysaccharide molecule. in order to fully understand the mechanisms of bacterial infection and host recognition a full structure/activity study of lipopolysaccharide is needed. in the present paper, we define the complete structure of the o-specific polysaccharide from the lipopolysacc ... | 2009 | 19589504 |
| identification of burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody. | the genus burkholderia includes many bacteria that cause serious human infections. as is the case with other gram-negative bacteria, burkholderia species produce lps, which is an abundant component of the bacterial cell surface. burkholderia cepacia complex (bcc) bacteria (which include at least 17 separate species) produce lps structures that are quite different. in an attempt to determine the degree of lps epitope variation among bcc species, a mab was produced, designated 5d8, specific for th ... | 2010 | 19729457 |
| igneous phosphate rock solubilization by biofilm-forming mycorrhizobacteria and hyphobacteria associated with rhizoglomus irregulare daom 197198. | biofilm formation on abiotic and biotic surfaces was studied with two hyphobacteria, strongly attached to the surface of the arbuscular mycorrhizal fungus (amf) rhizoglomus irregulare (ri) daom 197198 and two mycorrhizobacteria, loosely attached to the roots of different mycorrhizal plants. when the sparingly soluble igneous phosphate rock (pr) from quebec, or when the chemical hydroxyapatite were used as sole phosphorus (p) source, hyphobacteria rhizobium miluonense rm3 and burkholderia anthina ... | 2016 | 27541158 |
| synthesis of a trisaccharide repeating unit of the o-antigen from burkholderia anthina and its dimer. | a trisaccharide repeating unit of the o-antigen from burkholderia anthina, α-l-rha-(1→2)-α-l-rha-(1→2)-β-d-gal-o(ch2)3nh2 (1), and its dimer, α-l-rha-(1→2)-α-l-rha-(1→2)-α-d-gal-(1→3)-α-l-rha-(1→2)-α-l-rha-(1→2)-β-d-gal-o(ch2)3nh2 (2), were synthesized via a highly convergent and efficient assembly strategy. sequential glycosylation of galactosyl acceptor 6 with rhamnosyl thioglycoside 7, followed by condensation of the resulting disaccharide acceptor 9 with rhamnosyl imidate donor 10, gave the ... | 2016 | 27085165 |
| efficient synthesis of o-antigen fragments expressed by burkholderia anthina by modular synthesis approach. | to facilitate mapping of the interaction region of the o-chain of the lipopolysaccharide from burkholderia anthina and of a lipopolysaccharide-specific monoclonal antibody, trisaccharide propyl α-l-rhamnopyranosyl-(1 → 2)-α-d-galactopyranosyl-(1 → 3)-α-l-rhamnopyranoside (27) and hexasaccharide propyl α-l-rhamnopyranosyl-(1 → 2)-α-d-galactopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 2)-α-d-galactopyranosyl-(1 → 3)-α-l-rhamnopyranoside (33) were synthesized. these oligo ... | 2015 | 25665786 |
| unraveling the interaction between the lps o-antigen of burkholderia anthina and the 5d8 monoclonal antibody by using a multidisciplinary chemical approach, with synthesis, nmr, and molecular modeling methods. | the interaction between the o-chain from the lipopolysaccharide from burkholderia anthina and a lipopolysaccharide-specific monoclonal antibody (5d8) has been studied at high resolution by nmr spectroscopy. in particular, the 5d8-bound epitope of the saccharide entity has been unraveled by a combination of saturation transfer difference (std) and transferred noesy (tr-noesy) experiments performed on the 5d8/polysaccharide complex. to dissect the fine details of the molecular recognition events, ... | 2013 | 23873779 |