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cdna cloning of the housefly pigment-dispersing factor (pdf) precursor protein and its peptide comparison among the insect circadian neuropeptides.pigment-dispersing factor (pdf), an 18-amino acid neuropeptide, is a principal circadian neurotransmitter for the circadian rhythms of the locomotor activity in flies. recently, two completely different types of pdf precursor were clarified; that of the cricket gryllus bimaculatus and that of the last-summer cicada meimuna opalifera. the g. bimaculatus pdf precursor is extraordinarily short and comprises a nuclear localization signal (nls), while the m. opalifera pdf precursor is of ordinary len ...200414994986
a circadian neuropeptide, pigment-dispersing factor-pdf, in the last-summer cicada meimuna opalifera: cdna cloning and immunocytochemistry.pigment-dispersing factor (pdf), an 18-amino acid neuropeptide, is a principal circadian neuromodulator functioning downstream of the insect brain's circadian clock, modulating daily rhythms of locomotor activity. recently, we found that pdf precursors of the cricket gryllus bimaculatus comprise a nuclear localization signal (nls). moreover, the nuclear localization of pdf immunoreactivity and the translocation of gfp-fused pdf precursor into the nucleus have both been demonstrated. these sugges ...200212193798
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