antifungal activity of some marine organisms from india, against food spoilage aspergillus strains.crude aqueous methanol extracts obtained from 31 species of various marine organisms (including floral and faunal), were screened for their antifungal activity against food poisoning strains of aspergillus. seventeen species exhibited mild (+ = zone of inhibition 1-2 mm) to significant (+3 = zone of inhibition 3-5 mm) activity against one or the other strain under experiment. however, extracts of 12 species were active against all the three strains. organisms like salicornia brachiata (obligate ...199911040863
antimicrobial activity of fatty acid methyl esters of some members of chenopodiaceae.fatty acid methyl ester (fame) extracts of four halophytic plants, viz. arthrocnemum indicum, salicornia brachiata, suaeda maritima and suaeda monoica belonging to the family chenopodiaceae, were prepared and their composition was analyzed by gc-ms. the fame extracts were also screened for antibacterial and antifungal activities. the gc-ms analysis revealed the presence of more saturated fatty acids than unsaturated fatty acids. among the fatty acids analyzed, the relative percentage of lauric a ...200818669016
spectroscopy investigation on chemo-catalytic, free radical scavenging and bactericidal properties of biogenic silver nanoparticles synthesized using salicornia brachiata aqueous extract.nanosized silver have been widely used in many applications, such as catalysis, photonics, sensors, medicine etc. thus, there is an increasing need to develop high-yield, low cost, non-toxic and eco-friendly procedures for the synthesis of nanoparticles. herein, we report an efficient, green synthesis of silver nanoparticles utilizing the aqueous extract of salicornia brachiata, a tropical plant of the chenopodiaceae family. silver nanoparticles have been characterized by ultraviolet-visible spe ...201424056313
cloning and transcript analysis of type 2 metallothionein gene (sbmt-2) from extreme halophyte salicornia brachiata and its heterologous expression in e. coli.salicornia brachiata is an extreme halophyte growing luxuriantly in the coastal marshes and frequently exposed to various abiotic stresses including heavy metals. a full length type 2 metallothionein (sbmt-2) gene was isolated using race and its copy number was confirmed by southern blot analysis. transcript expression of sbmt-2 gene was analyzed by semi-quantitative rt-pcr and real time quantitative (qrt) pcr. expression of sbmt-2 gene was up-regulated concurrently with zinc, copper, salt, heat ...201222441126
brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth promoting potential.a gram-positive, aerobic, non-motile, coccoid shaped, halotolerant bacterium (jg 06 t) was isolated from roots of salicornia brachiata, an extreme halophyte. phylogenetic analysis based on 16s rrna gene sequence showed sequence similarities of 99.2% to brachybacterium paraconglomeratum, 99.0% to b. conglomeratum, and 98.2% to b. faecium. dna-dna hybridization with the close relatives brachybacterium paraconglomeratum (dsm 46341t), b. conglomeratum (dsm 10241 t), b. faecium (dsm 4810t), b. tyrofe ...201121216918
sbdreb2a, an a-2 type dreb transcription factor from extreme halophyte salicornia brachiata confers abiotic stress tolerance in escherichia coli.dehydration-responsive element binding (dreb) transcription factor plays a key role in plant stress signal transduction pathway. in this study, sbdreb2a has been isolated from the halophyte salicornia brachiata. sbdreb2a cdna is 1,062 bp long, encoding protein of 353 amino acids with an estimated molecular mass of 39.37 kda and a pi of 4.98. on the basis of multiple sequence alignment and phylogenetic analysis, sbdreb2a is classified in a-2 group of the dreb family. the genomic organization conf ...201020640426
zhihengliuella somnathii sp. nov., a halotolerant actinobacterium from the rhizosphere of a halophyte salicornia brachiata.two novel, gram-stain-positive, rod-shaped, halotolerent bacteria, strains jg 03(t) and jg 05 were isolated from the rhizosphere of salicornia brachiata, an extreme halophyte. comparative analyses of 16s rrna gene sequences showed that they were closely related to members of the genus zhihengliuella, with sequence similarities of 96.9-99.1%. the sequence similarity of strains jg 03(t )and jg 05 with each other was 99.4%. dna-dna hybridization of jg 03(t) and jg 05 with other species of the genus ...201526297009
ectopic over-expression of peroxisomal ascorbate peroxidase (sbpapx) gene confers salt stress tolerance in transgenic peanut (arachis hypogaea).peroxisomal ascorbate peroxidase gene (sbpapx) of an extreme halophyte salicornia brachiata imparts abiotic stress endurance and plays a key role in the protection against oxidative stress. the cloned sbpapx gene was transformed to local variety of peanut and about 100 transgenic plants were developed using optimized in vitro regeneration and agrobacterium mediated genetic transformation method. the t0 transgenic plants were confirmed for the gene integration; grown under controlled condition in ...201424954532
introgression of the sbasr-1 gene cloned from a halophyte salicornia brachiate enhances salinity and drought endurance in transgenic groundnut (arachis hypogaea)and acts as a transcription factor [corrected].the sbasr-1 gene, cloned from a halophyte salicornia brachiata, encodes a plant-specific hydrophilic and stress responsive protein. the genome of s. brachiata has two paralogs of the sbasr-1 gene (2549 bp), which is comprised of a single intron of 1611 bp, the largest intron of the  abscisic acid stress ripening [asr] gene family yet reported. in silico analysis of the 843-bp putative promoter revealed the presence of aba, biotic stress, dehydration, phytohormone, salinity, and sugar responsive ...201526158616
correction: introgression of the sbasr-1 gene cloned from a halophyte salicornia brachiata enhances salinity and drought endurance in transgenic groundnut (arachis hypogaea) and acts as a transcription factor. 201526252387
a snare-like superfamily protein sbslsp from the halophyte salicornia brachiata confers salt and drought tolerance by maintaining membrane stability, k(+)/na(+) ratio, and antioxidant machinery.about 1000 salt-responsive ests were identified from an extreme halophyte salicornia brachiata. among these, a novel salt-inducible gene sbslsp (salicornia brachiata snare-like superfamily protein), showed up-regulation upon salinity and dehydration stress. the presence of cis-regulatory motifs related to abiotic stress in the putative promoter region supports our finding that sbslsp gene is inducible by abiotic stress. the sbslsp protein showed a high sequence identity to hypothetical/uncharact ...201627313584
molecular characterization of an myb transcription factor from a succulent halophyte involved in stress tolerance.abiotic stresses like drought, salinity and extreme temperature significantly affect crop productivity. plants respond at molecular, cellular and physiological levels for management of stress tolerance. functional and regulatory genes play a major role in controlling these abiotic stresses through an intricate network of transcriptional machinery. transcription factors are potential tools for manipulating stress tolerance since they control a large number of downstream genes. in the present stud ...201525986050
a novel salt-inducible gene sbsi-1 from salicornia brachiata confers salt and desiccation tolerance in e. coli.salicornia brachiata is one of the extreme salt tolerant plants and grows luxuriantly in coastal areas. previously we have reported isolation and characterization of ests from s. brachiata with large number of unknown gene sequences. reverse northern analysis showed upregulation and downregulation of few unknown genes in response to salinity. some of these unknown genes were made full length and their functional analysis is being tested. in this study, we have selected a novel unknown salt induc ...201221655957
heterologous expression of an uncharacterized universal stress protein gene (sbusp) from the extreme halophyte, salicornia brachiata, which confers salt and osmotic tolerance to e. coli.salicornia brachiata is an extreme halophyte considered to be a rich source of stress responsive genes and an est database revealed that 30% of its genes are uncharacterized. in order to ascertain its function, a gene (sal-e-56) of unknown function was made full length using race, cloned and characterized. the full length gene (873 bp; accession no. kf164282) contained an open reading frame (orf) of 486 bp encoding for a protein that belongs to the universal stress protein (usp) family that was ...201424291028
a novel gene sbsi-2 encoding nuclear protein from a halophyte confers abiotic stress tolerance in e. coli and tobacco.salicornia brachiata is an extreme halophyte that grows luxuriantly in coastal marshes. previously, we have reported isolation and characterization of ests from salicornia with large number of novel/unknown salt-responsive gene sequences. in this study, we have selected a novel salt-inducible gene sbsi-2 (salicornia brachiata salt-inducible-2) for functional characterization. bioinformatics analysis revealed that sbsi-2 protein has predicted nuclear localization signals and a strong protein-prot ...201424999628
expression of sbgstu (tau class glutathione s-transferase) gene isolated from salicornia brachiata in tobacco for salt tolerance.tau class glutathione transferases (gstu) genes are plant specific, induced by different abiotic stress, and important for protecting plants against oxidative damage. gst gene was isolated using 5' race from an extreme halophyte salicornia brachiata, cloned, sequenced and its protein structure was predicted. transcript profiling of sbgst gene expression was studied under different abiotic stress conditions and plant growth regulator treatments, viz. salt, cold, drought, aba and salicylic acid, w ...201021136169
cloning and characterization of the salicornia brachiata na(+)/h(+) antiporter gene sbnhx1 and its expression by abiotic stress.salinity causes multifarious adverse effects to plants. plants response to salt stress involves numerous processes that function in coordination to alleviate both cellular hyperosmolarity and ion disequilibrium. a na(+)/h(+) antiporter nhx1 gene has been isolated from a halophytic plant salicornia brachiata in this study. predicted amino acid sequence similarity, protein topology and the presence of functional domains conserved in sbnhx1 classify it as a plant vacuolar nhx gene. the sbnhx1 cdna ...201120853145
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