| opitutus terrae gen. nov., sp. nov., to accommodate novel strains of the division 'verrucomicrobia' isolated from rice paddy soil. | three strains of obligately anaerobic bacteria were isolated from rice paddy soil microcosms. comparative analysis of the 16s rrna genes showed that these novel isolates have identical gene sequences and are members of the division 'verrucomicrobia'. the novel strains are phenotypically and phylogenetically distinct from species described previously. one strain, pb90-1t, was characterized in more detail. the cells are cocci and are motile by means of a flagellum. catalase and oxidase activities ... | 2001 | 11760935 |
| propionate formation by opitutus terrae in pure culture and in mixed culture with a hydrogenotrophic methanogen and implications for carbon fluxes in anoxic rice paddy soil. | propionate-forming bacteria seem to be abundant in anoxic rice paddy soil, but biogeochemical investigations show that propionate is not a correspondingly important intermediate in carbon flux in this system. mixed cultures of opitutus terrae strain pb90-1, a representative propionate-producing bacterium from rice paddy soil, and the hydrogenotrophic methanospirillum hungatei strain sk maintained hydrogen partial pressures similar to those in the soil. the associated shift away from propionate f ... | 2002 | 11916740 |
| puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of puniceicoccaceae fam. nov., puniceicoccales ord. nov., opitutaceae fam. nov., opitutales ord. nov. and opitutae classis nov. in the phylum 'verrucomicrobia'. | a gram-negative, chemoheterotrophic, facultatively anaerobic coccus, designated imcc1545(t), was isolated from the digestive tract of a marine clamworm, periserrula leucophryna, inhabiting a tidal flat of the yellow sea. cells of strain imcc1545(t) are non-motile, dividing by binary fission. the predominant fatty acids are anteiso-c(15 : 0) and c(18 : 0). the respiratory quinone is menaquinone-7 and the dna g+c content is 52.1 mol%. phylogenetic analyses based on 16s rrna gene sequences using th ... | 2007 | 17329779 |
| development of a 16s rrna-targeted probe set for verrucomicrobia and its application for fluorescence in situ hybridization in a humic lake. | members of the highly diverse bacterial phylum verrucomicrobia are globally distributed in various terrestrial and aquatic habitats. they are key players in soils, but little is known about their role in aquatic systems. here, we report on the design and evaluation of a 16s rrna-targeted probe set for the identification of verrucomicrobia and of clades within this phylum. subsequently, the probe set was applied to a study concerning the seasonal abundance of verrucomicrobia in waters of the humi ... | 2010 | 20226613 |
| genome sequence of the verrucomicrobium opitutus terrae pb90-1, an abundant inhabitant of rice paddy soil ecosystems. | bacteria of the deeply branching phylum verrucomicrobia are rarely cultured yet commonly detected in metagenomic libraries from aquatic, terrestrial, and intestinal environments. we have sequenced the genome of opitutus terrae pb90-1, a fermentative anaerobe within this phylum, isolated from rice paddy soil and capable of propionate production from plant-derived polysaccharides. | 2011 | 21398538 |
| Genomic and Physiological Characterization of the Verrucomicrobia Isolate Diplosphaera colotermitum gen. nov., sp. nov. Reveals Microaerophily and Nitrogen Fixation Genes. | Previously we reported the cultivation of novel verrucomicrobia, including strain TAV2 (93% 16S rRNA gene identity to its nearest cultivated representative, Opitutus terreae PB90-1) from the gut of the termite Reticulitermes flavipes. To gain better insight into the Verrucomicrobia as a whole and understand the role of verrucomicrobia within the termite gut ecosystem, we analyzed a draft genome and undertook a physiological characterization of TAV2. Strain TAV2 is an autochthonous member of the ... | 2011 | 22194293 |