| isolation of microorganisms for biological detoxification of lignocellulosic hydrolysates. | acid pretreatment of lignocellulosic biomass releases furan and phenolic compounds, which are toxic to microorganisms used for subsequent fermentation. in this study, we isolated new microorganisms for depletion of inhibitors in lignocellulosic acid hydrolysates. a sequential enrichment strategy was used to isolate microorganisms from soil. selection was carried out in a defined mineral medium containing a mixture of ferulic acid (5 mm), 5-hydroxymethylfurfural (5-hmf, 15 mm), and furfural (20 m ... | 2004 | 12908085 |
| bioabatement to remove inhibitors from biomass-derived sugar hydrolysates. | bioabatement is a potential method to remove inhibitory compounds from lignocellulose hydrolysates that could be incorporated into a scheme for fermentation of ethanol from cellulose. coniochaeta ligniaria nrrl30616, an ascomycete that metabolizes furfural and 5-hydroxymethylfurfural, is a unique strain that may be useful for detoxifying biomass sugars. nrrl30616 and 23 related fungal strains were screened for the ability to metabolize furans and grow in dilute-acid hydrolysate of corn stover. n ... | 2005 | 15917615 |
| removal of phytotoxic compounds from torrefied grass fibres by plant-beneficial microorganisms. | we aimed to select microorganisms colonizing torrefied grass fibres (tgf) and simultaneously reducing the phytotoxicity which appeared after heat treatment of the fibres. eighty-eight bacterial strains and one fungus, previously isolated from a sequential enrichment experiment on torrefied fibres and extracts, were tested separately for their capacity to decrease phytotoxicity. eleven of the bacterial strains and the fungus significantly reduced phytotoxicity. these organisms were checked for th ... | 2008 | 18537835 |
| interactions of plant-beneficial bacteria with the ascomycete coniochaeta ligniaria. | to assess the interactions between coniochaeta ligniaria f/tgf15 obtained from torrefied grass fibers (tgf) and selected bacteria from the same substrate. | 2009 | 19298515 |
| microbial enrichment of a novel growing substrate and its effect on plant growth. | the quality of torrefied grass fibers (tgf) as a new potting soil ingredient was tested in a greenhouse experiment. tgf was colonized with previously selected microorganisms. four colonization treatments were compared: (1) no inoculants, (2) the fungus coniochaeta ligniaria f/tgf15 alone, (3) the fungus followed by inoculation with two selected bacteria, and (4) the fungus with seven selected bacteria. cultivation-based and dna-based methods, i.e., pcr-dgge and box-pcr, were applied to assess th ... | 2009 | 19387721 |
| fermentation of bioenergy crops into ethanol using biological abatement for removal of inhibitors. | biological abatement was used to condition dilute acid-pretreated hydrolysates of three perennial herbaceous crops that are potential bioenergy feedstocks: switchgrass, reed canarygrass, and alfalfa stems. fungal isolate coniochaeta ligniaria was inoculated into the hydrolysates to metabolize and remove inhibitory compounds prior to yeast fermentation of glucose. switchgrass, reed canarygrass, and alfalfa stem samples were pretreated with dilute acid at 10% w/w biomass loading and subjected to b ... | 2010 | 20510606 |
| biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review. | one of the major challenges faced in commercial production of lignocellulosic bioethanol is the inhibitory compounds generated during the thermo-chemical pre-treatment step of biomass. these inhibitory compounds are toxic to fermenting micro-organisms. the ethanol yield and productivity obtained during fermentation of lignocellulosic hydrolysates is decreased due to the presence of inhibiting compounds, such as weak acids, furans and phenolic compounds formed or released during thermo-chemical p ... | 2011 | 20513164 |
| transformation and electrophoretic karyotyping of coniochaeta ligniaria nrrl30616. | coniochaeta ligniaria nrrl30616 is an ascomycete that grows with yeast-like appearance in liquid culture. the strain has potential utility for conversion of fibrous biomass to fuels or chemicals. furans and other inhibitory compounds in lignocellulosic biomass are metabolized by nrrl30616, facilitating subsequent microbial fermentation of biomass sugars. this study undertook initial characterization of the genetic system of c. ligniaria nrrl30616. transformation using hygromycin as a dominant se ... | 2011 | 21222124 |
| comparison of separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes for ethanol production from wheat straw by recombinant escherichia coli strain fbr5. | ethanol production by recombinant escherichia coli strain fbr5 from dilute acid pretreated wheat straw (ws) by separate hydrolysis and fermentation (shf) and simultaneous saccharification and fermentation (ssf) was studied. the yield of total sugars from dilute acid (0.5% h(2)so(4)) pretreated (160 °c, 10 min) and enzymatically saccharified (ph 5.0, 45 °c, 72 h) ws (86 g/l) was 50.0 ± 1.4 g/l. the hydrolyzate contained 1,184 ± 19 mg furfural and 161 ± 1 mg hydroxymethyl furfural per liter. the r ... | 2011 | 21968655 |
| hydrothermal pretreatment of sugarcane bagasse using response surface methodology improves digestibility and ethanol production by ssf. | sugarcane bagasse was characterized as a feedstock for the production of ethanol using hydrothermal pretreatment. reaction temperature and time were varied between 160 and 200°c and 5-20 min, respectively, using a response surface experimental design. the liquid fraction was analyzed for soluble carbohydrates and furan aldehydes. the solid fraction was analyzed for structural carbohydrates and klason lignin. pretreatment conditions were evaluated based on enzymatic extraction of glucose and xylo ... | 2011 | 22080307 |