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sex determination in 58 bird species and evaluation of chd gene as a universal molecular marker in bird sexing.the aim of this research was to test the chd gene (chromo helicase dna-binding gene) as a universal molecular marker for sexing birds of relatively distant species. the chd gene corresponds to the aim because of its high degree of conservation and different lengths in z and w chromosomes due to different intron sizes. dna was isolated from feathers and the amplification of the chd gene was performed with the following sets of polymerase chain reaction (pcr) primers: 2550f/2718r and p2/p8. sex de ...201522553188
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