| molecular characterization of a ranavirus isolated from largemouth bass micropterus salmoides. | an iridovirus, isolated from largemouth bass micropterus salmoides following a die-off among adult fish and provisionally designated largemouth bass virus (lmbv), was characterized by analysis of viral protein synthesis in infected cells, viral dna restriction fragment length polymorphisms (rflp), and sequence determination of the major capsid protein and viral dna methyltransferase genes. all 3 approaches yielded results consistent with the suggestion that lmbv was a member of the genus ranavir ... | 1999 | 10494500 |
| preliminary description of lesions in juvenile largemouth bass injected with largemouth bass virus. | juvenile largemouth bass micropterus salmoides were intraperitoneally injected with largemouth bass virus (lmbv), a member of the genus ranavirus, family iridoviridae. moribund fish which had been injected with 10(6.2) tissue culture infectious doses, 50% endpoint (tcid50), were sampled 4 d after injection; other largemouth bass injected with this dose died between 3 and 5 d after injection. fish injected with 10(2.8) tcid50 of lmbv were also examined after 4 d and had lesions similar to those o ... | 2000 | 10715819 |
| first isolation of largemouth bass virus. | the first reported fish kill caused by largemouth bass virus (lmbv) occurred in 1995 in santee-cooper reservoir, south carolina, usa. subsequently, this iridovirus has been implicated in additional fish kills and has also been found in clinically healthy fish in numerous locations in the southeastern usa. we compared the virus from santee-cooper reservoir with a virus isolated in 1991 from large-mouth bass, micropterus salmoides, from lake weir, florida. restriction fragment length polymorphisms ... | 2002 | 12219979 |
| characterization of a novel ranavirus isolated from grouper epinephelus tauvina. | a large icosahedral virus was isolated from diseased grouper epinephelus tauvina. the virus grew well in several cultured fish cell lines, with stable and high infectivity after serial passages in grouper cell line (gp). the virus was sensitive to both acid and heat treatments. virus replication was inhibited by 5-iodo-2-deoxyuridine (iudr), indicative of a dna-containing genome. the virus infectivity was reduced with ether treatment, suggesting that the virus was lipid-enveloped. electron micro ... | 2003 | 12608562 |
| pcr method for detection of largemouth bass virus. | a polymerase chain reaction (pcr) method was developed for largemouth bass virus (lmbv). this iridovirus can cause a lethal disease of largemouth bass micropterus salmoides, but also subclinically infects largemouth bass and other species of fishes. oligonucleotide primers were designed to specifically amplify the major capsid protein gene of lmbv. the protocol for sample processing and pcr provided a method that was more sensitive than cell culture for detection of lmbv in fish. the specific am ... | 2003 | 12718467 |
| strain variation in an emerging iridovirus of warm-water fishes. | although iridoviruses vary widely within and among genera with respect to their host range and virulence, variation within iridovirus species has been less extensively characterized. this study explores the nature and extent of intraspecific variation within an emerging iridovirus of north american warm-water fishes, largemouth bass virus (lmbv). three lmbv isolates recovered from three distinct sources differed genetically and phenotypically. genetically, the isolates differed in the banding pa ... | 2003 | 12885900 |
| a broadly applicable method to characterize large dna viruses and adenoviruses based on the dna polymerase gene. | many viral pathogens are poorly characterized, are difficult to culture or reagents are lacking for confirmatory diagnoses. we have developed and tested a robust assay for detecting and characterizing large dna viruses and adenoviruses. the assay is based on the use of degenerate pcr to target a gene common to these viruses, the dna polymerase, and sequencing the products. | 2006 | 16608526 |
| quantitative polymerase chain reaction assay for largemouth bass virus. | the use of quantitative polymerase chain reaction (qpcr) to test for largemouth bass virus (lmbv) was evaluated during a challenge experiment in which largemouth bass micropterus salmoides were immersed in the type strain of lmbv. the real-time pcr and cell culture methods were both used to measure lmbv present in the inoculum. additional samples tested by qpcr included gill, gonad, kidney, liver, mucus, spleen, and swim bladder. a plasmid clone containing a 248-base pair (bp) fragment of the ma ... | 2007 | 18333479 |
| a survey to determine the presence and distribution of largemouth bass virus in wild freshwater bass in new york state. | during 2004 and 2005 a survey was conducted to investigate the presence and geographic distribution of largemouth bass virus (lmbv) in new york state. this iridovirus is widely distributed across the eastern united states; however, it had not previously been reported in new york state. two hundred and eighty-three wild largemouth bass micropterus salmoides and 8 smallmouth bass m. dolomieu were collected from 37 locations across the state. no clinical signs of lmbv or mortalities attributable to ... | 2008 | 18942592 |
| largemouth bass virus in texas: distribution and management issues. | in response to fish kills at prominent fishing sites for largemouth bass micropterus salmoides, such as lake fork and sam rayburn reservoir, the texas parks and wildlife department began a systematic evaluation of state waters for the presence of largemouth bass virus (lmbv). the survey comprised 49 water bodies and 13 river basins, and a total of 2,876 adult bass were collected by electrofishing and angling during the summer and fall of 2000. the virus was initially detected by means of cell cu ... | 2009 | 19485124 |
| the effects of largemouth bass virus on a quality largemouth bass population in arkansas. | a 22.4-ha impoundment experienced an outbreak of largemouth bass (micropterus salmoides) virus (lmbv) disease in the summer of 2006. all dead or dying largemouth bass observed throughout the entire event were recorded and removed. in this study, we estimated mortality and examined size distribution, condition, and biomass following the outbreak. boat-mounted electrofishing was used to collect largemouth bass for a mark-recapture population estimate and other population metrics. fish samples were ... | 2009 | 19617487 |
| potential for largemouth bass virus to associate with and gain protection from bacterial biofilms. | quantitative pcr (qpcr) was used to investigate whether largemouth bass virus (lmbv) can exist within biofilms. suspended lmbv was partitioned into either laboratory-grown pseudomonas fluorescens biofilms or pond-grown, mixed-population biofilms. biofilm-entrapped lmbv retained infectivity when tested on epithelioma papillosum cyprini tissue culture cells. the lmbv associated with p. fluorescens biofilms were resistant to disinfection by sodium hypochlorite and an iodine-based compound (betadine ... | 2010 | 20848884 |
| mortality of centrarchid fishes in the potomac drainage: survey results and overview of potential contributing factors. | skin lesions and spring mortality events of smallmouth bass micropterus dolomieu and selected other species were first noted in the south branch of the potomac river in 2002. since that year morbidity and mortality have also been observed in the shenandoah and monocacy rivers. despite much research, no single pathogen, parasite, or chemical cause for the lesions and mortality has been identified. numerous parasites, most commonly trematode metacercariae and myxozoans; the bacterial pathogens aer ... | 2010 | 21192549 |
| major capsid protein gene sequence analysis of the santee-cooper ranaviruses dfv, gv6, and lmbv. | the santee-cooper ranaviruses doctor fish virus (dfv), guppy virus 6 (gv6), and largemouth bass virus (lmbv) are members of the genus ranavirus within the family iridoviridae. the major capsid protein (mcp) is a main structural protein of iridoviruses and supports the differentiation and classification of ranaviruses. presently the complete sequence of the mcp gene is known for most ranaviruses with the exception of the santee-cooper ranaviruses. in the present study, the complete nucleotide seq ... | 2011 | 22132498 |
| differential autophagic effects triggered by five different vertebrate iridoviruses in a common, highly permissive mandarinfish fry (mff-1) cell model. | autophagy of five vertebrate iridoviruses, including one megalocytivirus (infectious spleen and kidney necrosis virus, isknv) and four ranaviruses (chinese giant salamander iridovirus, cgsiv; tiger frog virus, tfv; grouper iridovirus, giv; and largemouth bass virus, lmbv) were investigated in a common, highly permissive mandarinfish fry (mff-1) cell model. the results showed marked autophagosome formation in giv- and lmbv-infected cells but not in isknv-, cgsiv- and tfv-infected mff-1 cells. str ... | 2016 | 26748344 |
| outbreaks of ulcerative disease associated with ranavirus infection in barcoo grunter, scortum barcoo (mcculloch & waite). | in 2013, an outbreak of ulcerative disease associated with ranavirus infection occurred in barcoo grunter, scortum barcoo (mcculloch & waite), farms in thailand. affected fish exhibited extensive haemorrhage and ulceration on skin and muscle. microscopically, the widespread haemorrhagic ulceration and necrosis were noted in gill, spleen and kidney with the presence of intracytoplasmic eosinophilic inclusion bodies. when healthy barcoo grunter were experimentally challenged via intraperitoneal an ... | 2017 | 28111768 |
| involvement of the pi3k and erk signaling pathways in largemouth bass virus-induced apoptosis and viral replication. | increased reports demonstrated that largemouth bass, micropterus salmoides in natural and artificial environments were always suffered from an emerging iridovirus disease, largemouth bass virus (lmbv). however, the underlying mechanism of lmbv pathogenesis remained largely unknown. here, we investigated the cell signaling events involved in virus induced cell death and viral replication in vitro. we found that lmbv infection in epithelioma papulosum cyprini (epc) cells induced typical apoptosis, ... | 2014 | 25260912 |
| development and characterization of a largemouth bass cell line. | abstract the development and characterization of a new cell line, derived from the ovary of largemouth bass micropterus salmoides, is described. gonad tissue was collected from largemouth bass that were electrofished from oneida lake, new york. the tissue was processed and grown in culture flasks at approximately 22°c for more than 118 passages during an 8-year period from 2004 to 2011. the identity of these cells as largemouth bass origin was confirmed by sequencing a portion of the cytochrome ... | 2014 | 25229492 |
| isolation and characterization of a ranavirus from koi, cyprinus carpio l., experiencing mass mortalities in india. | we investigated mass mortalities of koi, cyprinus carpio linnaeus, 1758, experienced in south indian fish farms by virus isolation, electron microscopy, pcr detection, sequencing of capsid protein gene and transmission studies. samples of moribund koi brought to the laboratory suffered continuous mortality exhibiting swimming abnormalities, intermittent surfacing and skin darkening. irido-like virus was isolated from the infected fish in the indigenous snakehead kidney cell line (snkd2a). icosah ... | 2015 | 24720625 |
| identification of largemouth bass virus in the introduced northern snakehead inhabiting the chesapeake bay watershed. | the northern snakehead channa argus is an introduced species that now inhabits the chesapeake bay. during a preliminary survey for introduced pathogens possibly harbored by these fish in virginia waters, a filterable agent was isolated from five specimens that produced cytopathic effects in bf-2 cells. based on pcr amplification and partial sequencing of the major capsid protein (mcp), dna polymerase (dnapol), and dna methyltransferase (mtase) genes, the isolates were identified as largemouth ba ... | 2013 | 23895368 |
| occurrence of a lethal ranavirus in hybrid mandarin (siniperca scherzeri×siniperca chuatsi) in guangdong, south china. | a novel ranavirus with features similar to largemouth bass virus (lmbv) was isolated and then characterized from a natural mass mortality of adult hybrid mandarin (siniperca scherzeri×siniperca chuatsi). the isolated lmbv-like iridovirus was designated as mandarin ranavirus (mrv)-gd1301. the results of artificial infection showed that mrv-gd1301 was highly virulent to hybrid mandarin juveniles, and 100% mortality was observed within 5days after infection via intraperitoneal injection. moribund f ... | 2017 | 28619157 |
| characterization of a virulent ranavirus isolated from marine ornamental fish in india. | a viral agent implicated in the mortality of marine ornamental "similar damselfish" (pomacentrus similis allen, 1991) was isolated and characterized. the virus grew well in marine and freshwater fish cell lines from seabass and snakehead. the virus was sensitive to chloroform, acidic ph (3.0) and heat treatment at 56 °c. biochemical characterisation indicated that the virus had double stranded dna genome. transmission electron microscopic analysis of ultrathin sections of infected cell pellets s ... | 2017 | 29291228 |
| rapid diagnosis of largemouth bass ranavirus in fish samples using the loop-mediated isothermal amplification method. | largemouth bass ranavirus (lmbv) has been recognized as the causative pathogen responsible for infectious skin ulcerative syndrome in cultured largemouth bass in china. a fast and simple lmbv detection method is urgently needed. here, a loop-mediated isothermal amplification (lamp) assay was established for the detection of this virus using primers targeting the major capsid protein gene of lmbv. the amplification conditions were optimized; the assay was specific for the diagnosis of lmbv, as th ... | 2020 | 32268179 |