| viral susceptibility, transfection and growth of spb--a fish neural progenitor cell line from the brain of snubnose pompano, trachinotus blochii (lacépède). | this study investigates the susceptibilities of the spb cell line to fish viruses including giant seaperch iridovirus (gsiv-k1), red sea bream iridovirus (rsiv-ku), grouper nervous necrosis virus (gnnv-k1), chum salmon reovirus (csv) and eel herpesvirus (hva). gsiv-k1, rsiv-ku and csv replicated well in spb cells, with a significant cytopathic effect and virus production. however, the cells were hva and gnnv refractory. to examine the ability of spb cells to stably express foreign protein, expre ... | 2013 | 23305502 |
| giant seaperch iridovirus infection upregulates bas and bak expression, leading to apoptotic death of fish cells. | the giant seaperch iridovirus (gsiv) induces host cell apoptosis by a poorly-understood process. in this study, gsiv is shown to upregulate the pro-apoptotic death genes bax and bak at the middle replication stage, and factors in the grouper fin cell line (gf-1) are shown to modulate this process. studying the mechanism of cell death, we found that upregulated, de novo-synthesized bax and bak proteins formed heterodimers. this up-regulation process correlated with mitochondrial membrane potentia ... | 2015 | 26067170 |
| giant seaperch iridovirus (gsiv) induces mitochondria-mediated cell death that is suppressed by bongkrekic acid and cycloheximide in a fish cell line. | giant seaperch iridovirus (gsiv) induces cell death by an unknown mechanism. we postulated that this mechanism involves mitochondria-mediated cell death. cell viability assays revealed a steady increase in dead grouper fin cells (gf-1) after gsiv infection, from 11% at 2 days post-infection (dpi) to 67% at 5 dpi. annexin v/pi staining revealed gsiv infection induced apoptosis in a steadily increasing fraction of cells, from 4% at 1 dpi to 29% at 5 dpi. furthermore, post-apoptotic necrosis was ap ... | 2016 | 26548846 |
| gsiv serine/threonine kinase can induce apoptotic cell death via p53 and pro-apoptotic gene bax upregulation in fish cells. | previous studies have shown that gsiv induces apoptotic cell death through upregulation of the pro-apoptotic genes bax and bak in grouper fin cells (gf-1 cells). however, the role of viral genome-encoded protein(s) in this death process remains unknown. in this study, we demonstrated that the giant seaperch iridovirus (gsiv) genome encoded a serine/threonine kinase (st kinase) protein, and induced apoptotic cell death via a p53-mediated bax upregulation approach and a downregulation of bcl-2 in ... | 2016 | 26833308 |