| rapid mapping of transposon insertion and deletion mutations in the large ti-plasmids of agrobacterium tumefaciens. | a procedure is presented, that has allowed the rapid assignment of transposon tn1 and tn7 insertion sites in the large (130 md) nopaline ti-plasmid ptic58, to specific restriction enzyme fragments. total bacterial dna is isolated from agrobacterium tumefaciens strain c58 mutants that carry a transposon in their ti-plasmid, and digested with an appropriate restriction endonuclease. the fragments are separated on an agarose gel, denatured and transferred to nitrocellulose filters. these are hybrid ... | 1979 | 231764 |
| purification and characterization of the crown gall specific enzyme nopaline synthase. | nopaline synthase of sunflower (helianthus annuus l.) crown gall tissue induced by agrobacterium tumefaciens strain c58 or t37 (nopaline utilizers) was purified to homogeneity as judged by analytical disc gel electrophoresis. the native enzyme elutes from a column of ultrogen aca 34 as a single peak with an estimated molecular weight of 158,000. the dissociated enzyme migrates on nadodso4-polyacrylamide gels as a single band with a molecular weight of 40,000. thus, the native enzyme appears to b ... | 1979 | 476084 |
| transient transfection of mammalian cells with dna of the plant-pathogenic ti-plasmid and expression of marker and resident sequences. | the large ti-plasmid from agrobacterium tumefaciens strain c58 has been used for transfection experiments with mammalian cells. in dna from tupaia baby fibroblasts ti-plasmid sequences could be identified by filter hybridization as long as four weeks after transfection including two cell passages. the hybridization signals decreased rapidly after addition of the ti-plasmid dna-coprecipitate to the cells. the signals were often not detected any more after the first day, but were visible one week ... | 1989 | 2471056 |
| characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline ti plasmid ptic58. | overlapping segments of ptic58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine a and b agrobacterium tumefaciens strain c58. all of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines a and b. transposon tn3-hoho1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines a and b and ag ... | 1988 | 2832379 |
| a spontaneous insertion in the agrocin sensitivity region of the ti-plasmid of agrobacterium tumefaciens c58. | a spontaneous agrocin-resistant mutant of agrobacterium tumefaciens strain c58 was shown to have an insertion of 1.2 kb in the agrocin-sensitivity region of ptic58. the insertion was cloned from the ti-plasmid, and a subclone containing only dna internal to the insertion was used to probe the ti-plasmid and chromosomal dna of the wild-type strain c58. the probe showed homology to chromosomal sequences but showed no homology to wild-type ptic58. homology was also detected with chromosomal sequenc ... | 1986 | 3027729 |
| physical mapping of part of the tumorigenic deoxyribonucleic acid (t-dan) region of the tumour-inducing (ti) plasmid from agrobacterium tumefaciens strain c58 [proceedings]. | | 1980 | 6245970 |
| utilization of cellobiose and other beta-d-glucosides in agrobacterium tumefaciens. | agrobacterium tumefaciens strain c58 was able to utilize carbon from cellobiose and some other beta-d-glucosides as efficiently as from glucose. beta-d-glucoside utilization was partially inducible and the induction was subject to catabolite repression by glucose, independently of the presence of cyclic amp in the medium. it was also independent of ti plasmid-encoded functions. beta-d-glucosides were hydrolysed by a single, cytoplasmic and constitutively expressed beta-glucosidase, which was act ... | 1995 | 8525065 |
| tanshinone production in ti-transformed salvia miltiorrhiza cell suspension cultures. | transformed cell cultures of salvia miltiorrhiza were established by infecting sterile plantlets with agrobacterium tumefaciens strain c58. the transformed cells in suspension formed macroscopic clumps of cell aggregates up to 2-3 cm in size rather than homogeneous cell suspensions. these transformed cells grew well in hormone-free media. it was found that the b5 mediums supported the best growth while the 6,7-v medium promoted tanshinone production in the transformed cell suspension cultures. t ... | 1997 | 9470220 |
| an efficient method for the production of transgenic plants of peanut (arachis hypogaea l.) through agrobacterium tumefaciens-mediated genetic transformation. | cotyledon explants from mature peanut seeds (arachis hypogaea l.) were optimized to obtain adventitious shoot buds with high frequencies (>90%). efficient transformation of these cotyledons by using agrobacterium tumefaciens strain c58 carrying neomycin phosphotransferase ii (nptii) and ß-glucuronidase (gus; uida), or coat protein gene of the indian peanut clump virus (ipcvcp) and nptii on binary vectors (pbi121; prokii:ipcvcp) led to the production of a large percentage (55%) of transgenic plan ... | 2000 | 11011088 |
| elevated temperature differentially affects virulence, virb protein accumulation, and t-pilus formation in different agrobacterium tumefaciens and agrobacterium vitis strains. | that gene transfer to plant cells is a temperature-sensitive process has been known for more than 50 years. previous work indicated that this sensitivity results from the inability to assemble a functional t pilus required for t-dna and protein transfer to recipient cells. the studies reported here extend these observations and more clearly define the molecular basis of this assembly and transfer defect. t-pilus assembly and virulence protein accumulation were monitored in agrobacterium tumefaci ... | 2001 | 11698374 |
| evidence for high activity of xylem parenchyma and ray cells in the interface of host stem and agrobacterium tumefaciens-induced tumours of ricinus communis. | rapidly developing tumours at hypocotyls of ricinus communis, induced by agrobacterium tumefaciens strain c58, were characterized by strong differentiation of vascular bundles and their functional connection to the host bundles. the stem/tumour interface showed increased xylem, with numerous vessels accompanied by multiseriate unlignified rays. to know how nutrients efficiently accumulate in the tumour sink tissue, cell electropotentials (e(m)) in cross-sections were mapped. the measured cells w ... | 2002 | 11971925 |
| elucidation of the o-chain structure from the lipopolysaccharide of agrobacterium tumefaciens strain c58. | a linear homopolysaccharide built of 3-alpha-l-6dtalp residues, randomly acetylated at position c-4, is described for the o-specific polysaccharide of agrobacterium tumefaciens strain c58. this structure, determined by spectroscopical and chemical methods, is strictly correlated to that of rhizobium loti strain nzp2213, which differs for the degree and the position of o-acetylation. | 2003 | 12932373 |
| reexamining the role of the accessory plasmid patc58 in the virulence of agrobacterium tumefaciens strain c58. | isogenic strains of agrobacterium tumefaciens carrying ptic58, patc58, or both were constructed and assayed semiquantitatively and quantitatively for virulence and vir gene expression to study the effect of the large 542-kb accessory plasmid, patc58, on virulence. earlier studies indicate that the att (attachment) genes of a. tumefaciens are crucial in the ability of this soil phytopathogen to infect susceptible host plants. mutations in many att genes, notably attr and attd, rendered the strain ... | 2003 | 14551325 |
| full structural characterization of the lipid a components from the agrobacterium tumefaciens strain c58 lipopolysaccharide fraction. | for the first time, the complete structure of the lipid a from the lipopolysaccharide of an agrobacterium species is here reported. in particular, the structure of the lipid a from a. tumefaciens strain c58, a soil pathogen bacterium strictly related to rhizobiaceae, was determined. the structural study, carried out by chemical analysis, mass spectrometry, and nuclear magnetic resonance spectroscopy, revealed that lipid a fraction consisted of a mixture of species all sharing the bis-phosphoryla ... | 2004 | 15159382 |
| thermophilic, reversible gamma-resorcylate decarboxylase from rhizobium sp. strain mtp-10005: purification, molecular characterization, and expression. | we found the occurrence of thermophilic reversible gamma-resorcylate decarboxylase (gamma-rdc) in the cell extract of a bacterium isolated from natural water, rhizobium sp. strain mtp-10005, and purified the enzyme to homogeneity. the molecular mass of the enzyme was determined to be about 151 kda by gel filtration, and that of the subunit was 37.5 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; in other words, the enzyme was a homotetramer. the enzyme was induced specifically ... | 2004 | 15466039 |
| agrobacterium tumefaciens-mediated transformation of embryogenic tissue and transgenic plant regeneration in chamaecyparis obtusa sieb. et zucc. | a genetic transformation procedure for chamaecyparis obtusa was developed after co-cultivation of embryogenic tissues with disarmed agrobacterium tumefaciens strain c58/pmp90, which harbours the sgfp (synthetic green fluorescent protein) visual reporter and nptii (neomycin phoshotransferase ii) selectable marker genes. the highest transformation frequency was 22.5 independent transformed lines per dish (250 mg embryogenic tissue) following selection on kanamycin medium. transgenic plantlets were ... | 2004 | 15761663 |
| a biosystem for alginate metabolism in agrobacterium tumefaciens strain c58: molecular identification of atu3025 as an exotype family pl-15 alginate lyase. | the gram-negative bacterium sphingomonas sp. strain a1 (strain a1) has a peculiar biosystem to directly import and depolymerize a macromolecule, alginate, which is encoded by a cluster of genes on the genome. we identified five clustered orfs homologous to some genes of the strain a1 cluster in the genome of agrobacterium tumefaciens strain c58 (strain c58). these orfs are atu3021, atu3022, atu3023, and atu3024, encoding a putative sugar abc transporter system and atu3025, which encodes a putati ... | 2006 | 16545947 |
| plasmid-dependent temperature-sensitive phase in crown gall tumorigenesis. | two methods have been used to show that the temperature-sensitive (ts) genes for plasmid maintenance located on ptic58 are responsible for the presence of a specific 37 degrees c ts period during crown gall tumorigenesis. tumors induced by agrobacterium tumefaciens strain c58 become resistant to 32 degrees c by 96 hr after infection, indicating that the "inception phase" of crown gall is complete at that time. however, c58 tumors remain sensitive to 37 degrees c until 168 hr after infection. whe ... | 1980 | 16592822 |
| characteristics of teratomas regenerated in vitro from octopine-type crown gall. | crown galls induced by infection of tobacco plants with agrobacterium tumefaciens strain c58-cl(ptib6s3) were excised and cultured in vitro. after about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. leaf explants from shoots of tumor-line t5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. detached leaves from t5 shoots also synthesized octopine. these result ... | 1982 | 16662180 |
| cell wall invertase in tobacco crown gall cells : enzyme properties and regulation by auxin. | the cell wall invertase from an agrobacterium tumefaciens-transformed nicotiana tabacum cell line (sr1-c58) was purified. the heterogeneously glycosylated enzyme has the following properties: m(r) 63,000, ph optimum at 4.7, k(m sucrose) 0.6 millimolar (at ph 4.7), pl 9.5. enzyme activity is inhibited by micromolar concentrations of hgcl(2) but is insensitive to h(2)o(2), n-ethylmaleimide and dithiothreitol. upon transfer of transformed cells from the stationary phase to fresh medium, a cyclohexi ... | 1990 | 16667892 |
| crystallization and preliminary x-ray analysis of an exotype alginate lyase atu3025 from agrobacterium tumefaciens strain c58, a member of polysaccharide lyase family 15. | almost all alginate lyases depolymerize alginate in an endolytical fashion via a beta-elimination reaction. the alginate lyase atu3025 from agrobacterium tumefaciens strain c58, consisting of 776 amino-acid residues, is a novel exotype alginate lyase classified into polysaccharide lyase family 15. the enzyme was crystallized at 293 k by sitting-drop vapour diffusion with polyethylene glycol 4000 as a precipitant. preliminary x-ray analysis showed that the atu3025 crystal belonged to space group ... | 2006 | 16682783 |
| coupling two mercury resistance genes in eastern cottonwood enhances the processing of organomercury. | eastern cottonwood (populus deltoides bartr. ex marsh.) trees were engineered to express mera (mercuric ion reductase) and merb (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. earlier studies with arabidopsis thaliana and nicotiana tabacum showed that this gene combination resulted in more efficient detoxification of organomercurial compounds than did merb alone, but neither species is optimal for long-term field applications. leaf dis ... | 2007 | 17309680 |
| opine-based agrobacterium competitiveness: dual expression control of the agrocinopine catabolism (acc) operon by agrocinopines and phosphate levels. | agrobacterium tumefaciens strain c58 can transform plant cells to produce and secrete the sugar-phosphate conjugate opines agrocinopines a and b. the bacterium then moves in response to the opines and utilizes them as exclusive sources of carbon, energy, and phosphate via the functions encoded by the acc operon. these privileged opine-involved activities contribute to the formation of agrobacterial niches in the environment. we found that the expression of the acc operon is induced by agrocinopi ... | 2008 | 18344359 |
| regeneration of transgenic cryptomeria japonica d. don after agrobacterium tumefaciens-mediated transformation of embryogenic tissue. | a genetic transformation procedure for cryptomeria japonica was developed after co-cultivation of embryogenic tissues with the disarmed agrobacterium tumefaciens strain c58/pmp90, which harbours the visual reporter gene sgfp and two selectable marker genes, hpt and nptii. we were able to generate eight and three independent transgenic lines per gram of embryogenic tissue after selection on hygromycin and kanamycin medium, respectively. transgenic plants were regenerated through somatic embryogen ... | 2008 | 18542965 |
| cloning and characterization of uronate dehydrogenases from two pseudomonads and agrobacterium tumefaciens strain c58. | uronate dehydrogenase has been cloned from pseudomonas syringae pv. tomato strain dc3000, pseudomonas putida kt2440, and agrobacterium tumefaciens strain c58. the genes were identified by using a novel complementation assay employing an escherichia coli mutant incapable of consuming glucuronate as the sole carbon source but capable of growth on glucarate. a shotgun library of p. syringae was screened in the mutant e. coli by growing transformed cells on minimal medium containing glucuronic acid. ... | 2009 | 19060141 |