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blood clinical biochemistry and packed cell volume of the chinese three-striped box turtle, cuora trifasciata (reptilia: geoemydidae).reference intervals for blood biochemical analytes and packed cell volume are useful to veterinarians and conservation programs for monitoring the health of threatened species. populations of the critically endangered chinese three-striped box turtle, cuora trifasciata (bell, 1825), are heavily depleted in the wild because of overharvesting; and many individuals are now maintained in captive breeding programs. reference intervals of biochemical analytes and packed cell volume were established fo ...201425000682
separation and nanoencapsulation of antitumor peptides from chinese three-striped box turtle (cuora trifasciata).chinese three-striped box turtle (cuora trifasciata), as a freshwater turtle, is used as a tonic food. the purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. the results demonstrated that two fractions t1 and t2 exhibited good inhibition on hepg-2 and mcf-7 cancer cells, with an inhibition of 70.65-89.1%, at 500 μg/ml. subsequently, three peptides were identified from t1 and t2, including rgvkgpr (t1-1), klg ...201627292913
isolation and characterization of antiproliferative peptides from chinese three-striped box turtle (cuora trifasciata).in this study, the whole proteins from a chinese three-striped box turtle (cuora trifasciata) were extracted and hydrolyzed using three proteases (alcalase, papain, and protamex). by orthogonal experiments, the optimal hydrolysis conditions for producing peptides with the highest cancer cells growth inhibition activity were determined. such as, the maximum inhibition on mcf-7 cancer cells (92.37% at 1 mg/ml) was achieved by papain hydrolysis (ph 8, 37 °c, enzyme-to-substrate ratio (e/s) 1.5%), a ...201727957760
complete mitochondrial genome of cuora trifasciata (chinese three-striped box turtle), and a comparative analysis with other box turtles.cuora trifasciata has become one of the most critically endangered species in the world. the complete mitochondrial genome of c. trifasciata (chinese three-striped box turtle) was determined in this study. its mitochondrial genome is a 16,575-bp-long circular molecule that consists of 37 genes that are typically found in other vertebrates. and the basic characteristics of the c. trifasciata mitochondrial genome were also determined. moreover, a comparison of c. trifasciata with cuora cyclornata, ...201525445281
use of cystoscopy to visualize morphological alteration of the liver in a posthatchling turtle ( cuora trifasciata ).a 55 g, 7 mo old chinese three-striped box turtle ( cuora trifasciata ) was presented with a 2 wk history of lethargy and anorexia. the owner attempted various antibiotic treatments without clinical improvement. a cystoscopic evaluation of the coelom was performed. the liver appeared dark red-to-brown presenting multifocal irregularly shaped white-to-tan and roughly round areas of discoloration on the capsular surface. an adult keeled box turtle (pyxidea mouhotii), which died in the same facilit ...201627008324
the complete mitochondrial genome of the critically endangered vietnamese three-striped box turtle (testudines: geoemydidae).the complete mitochondrial genome of the vietnamese three-striped box turtle (cuora cyclornata) was first determined in this study. it was a circular molecule of 16,594 bp in length, consisting of 37 genes typically found in other vertebrates. the at content of the overall base composition of the whole mitogenome was 60.39%, while the control region was 70.23%. two etas and 4 csbs were identified, while a remarkable feature was found in the control region: a large number of (ttattata)10 direct t ...201524409888
molecular phylogeny of the critically endangered indochinese box turtle (cuora galbinifrons).the indochinese box turtle cuora galbinifrons is a polytypic, critically endangered species from vietnam, laos, and hainan island, china. we analyze up to 1790bp of mitochondrial dna under maximum parsimony and maximum likelihood criteria to test if the five historically recognized subspecies represent evolutionary lineages, and to elucidate the relationship of c. galbinifrons to other cuora. c. galbinifrons is composed of three major mitochondrial dna clades corresponding to the three subspecie ...200415019617
intervention on immunodeficiency mice and structural identification of enzymatic peptides from mauremys mutica and cuora trifasciata.mauremys mutica (asian yellow pond turtle, ypt) and cuora trifasciata (chinese three-striped box turtle, tbt) are traditional chinese medicine. they possess many biological characteristics, such as immune-enhancement, anti-inflammatory, anti-cancer effects. they have been used as folk anti-cancer drugs in central and southern china for a long time. however, there was no reports of comparing the immune-enhancement effect of ypt and tbt, nor of identifying the structures of ypt peptides and tbt pe ...201931042594
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